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Protein

Thrombospondin-4-B

Gene

thbs4b

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions and may be involved in various processes including cellular proliferation, migration, adhesion and attachment. May play a role in ER stress response (By similarity).By similarity

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • heparin binding Source: UniProtKB

GO - Biological processi

  • cell adhesion Source: UniProtKB-KW
  • positive regulation of cell division Source: UniProtKB-KW
  • response to unfolded protein Source: UniProtKB-KW
  • tendon development Source: ZFIN
Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Mitogen

Keywords - Biological processi

Cell adhesion, Unfolded protein response

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombospondin-4-B
Short name:
Thbs4-B
Gene namesi
Name:thbs4b
Synonyms:thbs4, tsp4
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)Imported
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-020708-4. thbs4b.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Extracellular matrix, Sarcoplasmic reticulum, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000003585523 – 949Thrombospondin-4-BAdd BLAST927

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi261InterchainPROSITE-ProRule annotationBy similarity
Disulfide bondi264InterchainPROSITE-ProRule annotationBy similarity
Disulfide bondi276 ↔ 287PROSITE-ProRule annotationBy similarity
Disulfide bondi281 ↔ 296PROSITE-ProRule annotationBy similarity
Disulfide bondi299 ↔ 310PROSITE-ProRule annotationBy similarity
Disulfide bondi316 ↔ 327PROSITE-ProRule annotationBy similarity
Disulfide bondi321 ↔ 336PROSITE-ProRule annotationBy similarity
Disulfide bondi339 ↔ 363PROSITE-ProRule annotationBy similarity
Disulfide bondi369 ↔ 383PROSITE-ProRule annotationBy similarity
Disulfide bondi377 ↔ 392PROSITE-ProRule annotationBy similarity
Disulfide bondi395 ↔ 407PROSITE-ProRule annotationBy similarity
Disulfide bondi413 ↔ 427PROSITE-ProRule annotationBy similarity
Disulfide bondi421 ↔ 437PROSITE-ProRule annotationBy similarity
Disulfide bondi439 ↔ 450PROSITE-ProRule annotationBy similarity
Disulfide bondi466 ↔ 471PROSITE-ProRule annotationBy similarity
Disulfide bondi476 ↔ 496PROSITE-ProRule annotationBy similarity
Disulfide bondi512 ↔ 532PROSITE-ProRule annotationBy similarity
Disulfide bondi535 ↔ 555PROSITE-ProRule annotationBy similarity
Disulfide bondi571 ↔ 591PROSITE-ProRule annotationBy similarity
Disulfide bondi594 ↔ 614PROSITE-ProRule annotationBy similarity
Glycosylationi601N-linked (GlcNAc...)Curated1
Disulfide bondi632 ↔ 652PROSITE-ProRule annotationBy similarity
Glycosylationi637N-linked (GlcNAc...)Curated1
Disulfide bondi672 ↔ 692PROSITE-ProRule annotationBy similarity
Disulfide bondi708 ↔ 929PROSITE-ProRule annotationBy similarity
Glycosylationi930N-linked (GlcNAc...)Curated1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8JGW0.
PRIDEiQ8JGW0.

Expressioni

Developmental stagei

Expressed both maternally and zygotically. Present at the gastrulation and post-segmentation stages.1 Publication

Interactioni

Subunit structurei

Homotrimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000093348.

Structurei

3D structure databases

ProteinModelPortaliQ8JGW0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 198Laminin G-likeAdd BLAST176
Domaini312 – 349EGF-like 1; calcium-bindingPROSITE-ProRule annotationAdd BLAST38
Domaini365 – 408EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST44
Domaini409 – 451EGF-like 3PROSITE-ProRule annotationAdd BLAST43
Repeati452 – 484TSP type-3 1Add BLAST33
Repeati485 – 520TSP type-3 2Add BLAST36
Repeati521 – 543TSP type-3 3Add BLAST23
Repeati544 – 579TSP type-3 4Add BLAST36
Repeati580 – 602TSP type-3 5Add BLAST23
Repeati603 – 640TSP type-3 6Add BLAST38
Repeati641 – 680TSP type-3 7Add BLAST40
Repeati681 – 716TSP type-3 8Add BLAST36
Domaini720 – 934TSP C-terminalPROSITE-ProRule annotationAdd BLAST215

Sequence similaritiesi

Belongs to the thrombospondin family.Curated
Contains 3 EGF-like domains.PROSITE-ProRule annotation
Contains 1 laminin G-like domain.Curated
Contains 1 TSP C-terminal (TSPC) domain.PROSITE-ProRule annotation
Contains 8 TSP type-3 repeats.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IFQQ. Eukaryota.
ENOG410XQKE. LUCA.
HOGENOMiHOG000007542.
HOVERGENiHBG000636.
InParanoidiQ8JGW0.
KOiK04659.
PhylomeDBiQ8JGW0.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR018097. EGF_Ca-bd_CS.
IPR001791. Laminin_G.
IPR024665. Thbs/COMP_coiled-coil.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
[Graphical view]
PfamiPF11598. COMP. 1 hit.
PF07645. EGF_CA. 2 hits.
PF02412. TSP_3. 6 hits.
PF05735. TSP_C. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 4 hits.
SM00179. EGF_CA. 2 hits.
SM00210. TSPN. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 3 hits.
PS01187. EGF_CA. 2 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8JGW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGTMHLLTA VSLILMLSSA NAESTVYNLL TSPDCLPDLL HGGLAEQGVT
60 70 80 90 100
ELYILTTFRI QPGTGNTIFS LYNPRDNSKY FEFSVFGKAN KAILRYLRRD
110 120 130 140 150
GRMSAVTFNK LNLADGEKHR LLFHLKGLEV GHPGGFPHSQ GALPVPGVEL
160 170 180 190 200
HLDCRLVETL RDLPAVFNGL NNHQAVELKT MQGKAQEGLE ELKLAYGDSV
210 220 230 240 250
ENVASLQDCH TQSDSVQALG LNTKQLTTQM LELTKVINEL KDVLIQQVKE
260 270 280 290 300
TSFLRNTISE CQACGLSGAE VVKPKCAPGV CFRDDMCIET AEGVECGPCP
310 320 330 340 350
DGYTGDGYSC DDVDECQFNP CFPGVRCVNM APGFRCEACP LGFTGKPLEG
360 370 380 390 400
VGVAYAQTHK QVCDDIDECK GPDNGGCTAN SICVNSVGSY QCGRCKTGFT
410 420 430 440 450
GDQIRGCKPE KSCGNRLQNP CDPNAQCTEE RDGTITCQCG IGWAGNGYLC
460 470 480 490 500
GKDTDIDGYP DERLRCRDPT CRKDNCVTVP NSGQEDADGD GKGDACDPDA
510 520 530 540 550
DGDGILNEQD NCWLTPNINQ QNSDKDSHGD ACDNCVRVDN PDQRDTDSDG
560 570 580 590 600
LGDACDDDMD GDGLKNFLDN CQRVKNRDQL DRDGDGVGDA CDSCPDIPNP
610 620 630 640 650
NQSDIDNDLV GDSCDTNQDS DGDGHQDSKD NCPMVINSSQ LDTDKDGIGD
660 670 680 690 700
ECDDDDDNDG IPDSLPPGPD NCRLVPNPEQ IDDNNDGVGD ICESDFDQDK
710 720 730 740 750
VIDRIDNCPE NAEITLTDFR AYQTVVLDPE GDAQIDPNWV VLNQGMEIVQ
760 770 780 790 800
TMNSDPGLAV GYTAFSGVDF EGTFHVNTVT DDDYAGFIFG YQDSSSFYVV
810 820 830 840 850
MWKQTEQTYW QATPFRAVAE PGIQLKAVKS KTGPGEHLRN SLWHTGDTND
860 870 880 890 900
QVRLLWKDPR NVGWKDKVSY RWYLQHRPQV GYIRVRFYEG TELVADSGVT
910 920 930 940
IDTTMRGGRL GVFCFSQENI IWSNLKYRCN DTIPEDFQEF STQHGMDPL
Length:949
Mass (Da):103,991
Last modified:October 1, 2002 - v1
Checksum:iBBC9203814CA99CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY081009 mRNA. Translation: AAL82733.1.
RefSeqiNP_775333.1. NM_173226.1.
UniGeneiDr.76718.

Genome annotation databases

GeneIDi252850.
KEGGidre:252850.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY081009 mRNA. Translation: AAL82733.1.
RefSeqiNP_775333.1. NM_173226.1.
UniGeneiDr.76718.

3D structure databases

ProteinModelPortaliQ8JGW0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000093348.

Proteomic databases

PaxDbiQ8JGW0.
PRIDEiQ8JGW0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi252850.
KEGGidre:252850.

Organism-specific databases

CTDi252850.
ZFINiZDB-GENE-020708-4. thbs4b.

Phylogenomic databases

eggNOGiENOG410IFQQ. Eukaryota.
ENOG410XQKE. LUCA.
HOGENOMiHOG000007542.
HOVERGENiHBG000636.
InParanoidiQ8JGW0.
KOiK04659.
PhylomeDBiQ8JGW0.

Miscellaneous databases

PROiQ8JGW0.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
4.10.1080.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR018097. EGF_Ca-bd_CS.
IPR001791. Laminin_G.
IPR024665. Thbs/COMP_coiled-coil.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
[Graphical view]
PfamiPF11598. COMP. 1 hit.
PF07645. EGF_CA. 2 hits.
PF02412. TSP_3. 6 hits.
PF05735. TSP_C. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 4 hits.
SM00179. EGF_CA. 2 hits.
SM00210. TSPN. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 3 hits.
PS01187. EGF_CA. 2 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTSP4B_DANRE
AccessioniPrimary (citable) accession number: Q8JGW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: October 1, 2002
Last modified: October 5, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.