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Protein

Protein disulfide-isomerase A3

Gene

PDIA3

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei55 – 551NucleophileBy similarity
Sitei56 – 561Contributes to redox potential valueBy similarity
Sitei57 – 571Contributes to redox potential valueBy similarity
Active sitei58 – 581NucleophileBy similarity
Sitei117 – 1171Lowers pKa of C-terminal Cys of first active siteBy similarity
Active sitei404 – 4041NucleophileBy similarity
Sitei405 – 4051Contributes to redox potential valueBy similarity
Sitei406 – 4061Contributes to redox potential valueBy similarity
Active sitei407 – 4071NucleophileBy similarity
Sitei469 – 4691Lowers pKa of C-terminal Cys of second active siteBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

ReactomeiR-GGA-901042. Calnexin/calreticulin cycle.
R-GGA-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase A3 (EC:5.3.4.1)
Alternative name(s):
Endoplasmic reticulum resident protein 57
Short name:
ER protein 57
Short name:
ERp57
Glucose-regulated thiol oxidoreductase 58 kDa protein
Gene namesi
Name:PDIA3
Synonyms:ERP57, GRP58
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 10

Subcellular locationi

  • Endoplasmic reticulum By similarity
  • Endoplasmic reticulum lumen By similarity
  • Melanosome By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 505481Protein disulfide-isomerase A3PRO_5000088911Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi55 ↔ 58Redox-activePROSITE-ProRule annotation
Disulfide bondi83 ↔ 90By similarity
Disulfide bondi404 ↔ 407Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ8JG64.
PRIDEiQ8JG64.

Interactioni

Protein-protein interaction databases

BioGridi674949. 1 interaction.
STRINGi9031.ENSGALP00000013574.

Structurei

3D structure databases

ProteinModelPortaliQ8JG64.
SMRiQ8JG64. Positions 25-486.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 131107Thioredoxin 1PROSITE-ProRule annotationAdd
BLAST
Domaini341 – 483143Thioredoxin 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi502 – 5054Prevents secretion from ERBy similarity

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated
Contains 2 thioredoxin domains.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0190. Eukaryota.
COG0526. LUCA.
GeneTreeiENSGT00760000119201.
HOGENOMiHOG000162459.
HOVERGENiHBG005920.
InParanoidiQ8JG64.
KOiK08056.
OMAiQINFAIA.
OrthoDBiEOG7VHSX1.
PhylomeDBiQ8JG64.
TreeFamiTF106382.

Family and domain databases

Gene3Di3.40.30.10. 3 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 1 hit.
PROSITEiPS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8JG64-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVPRPSRAA LLLLVPLLAL SAGASDVVEL SDADFESGLA ERPGLVLVEF
60 70 80 90 100
FAPWCGHCKR LAPEYEAAAT RLKGIVPLVK VDCTANSNTC NKYGVSGYPT
110 120 130 140 150
LKIFRDGEES GTYDGPRTAD GIVSHLKKQA GPASVALSSV ADFEKFIGDK
160 170 180 190 200
DASVVGFFRD ASGDAYSEFM KAANNLRDNY RFAHTSEEQL VQKYEEDGEG
210 220 230 240 250
VVLYRPSRLA NKFEDSTVKY TEDKITSAKI KKFIQENIFG ICPHMTEDNK
260 270 280 290 300
DLIQGKDLLV AYYDVDYEKN AKGSNYWRNR VMMIAKKFLD AGHKLSFAVA
310 320 330 340 350
SRKTFGHELS EFGLDNSVGE APVVAIRTAK GDKFVMQEEF SRDGKALERF
360 370 380 390 400
LQDYFDGNLK KYLKSEPVPE NNDGPVKVVV AENFDEIVNA EDKDVLIEFY
410 420 430 440 450
APWCGHCKNL EPKYKELGEK LSKDPNIVIA KMDATANDVP SPYEVRGFPT
460 470 480 490 500
IYFAPAGKKQ SPKKYEGGRE VSDFISYLKR EATSTPVLQE EDKAKKSKKK

AKEDL
Length:505
Mass (Da):56,182
Last modified:October 1, 2002 - v1
Checksum:i996509BBDC2D000A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY122886 mRNA. Translation: AAM82759.1.
RefSeqiNP_989441.1. NM_204110.3.
UniGeneiGga.3802.

Genome annotation databases

EnsembliENSGALT00000013589; ENSGALP00000013574; ENSGALG00000008348.
GeneIDi373899.
KEGGigga:373899.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY122886 mRNA. Translation: AAM82759.1.
RefSeqiNP_989441.1. NM_204110.3.
UniGeneiGga.3802.

3D structure databases

ProteinModelPortaliQ8JG64.
SMRiQ8JG64. Positions 25-486.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi674949. 1 interaction.
STRINGi9031.ENSGALP00000013574.

Proteomic databases

PaxDbiQ8JG64.
PRIDEiQ8JG64.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000013589; ENSGALP00000013574; ENSGALG00000008348.
GeneIDi373899.
KEGGigga:373899.

Organism-specific databases

CTDi2923.

Phylogenomic databases

eggNOGiKOG0190. Eukaryota.
COG0526. LUCA.
GeneTreeiENSGT00760000119201.
HOGENOMiHOG000162459.
HOVERGENiHBG005920.
InParanoidiQ8JG64.
KOiK08056.
OMAiQINFAIA.
OrthoDBiEOG7VHSX1.
PhylomeDBiQ8JG64.
TreeFamiTF106382.

Enzyme and pathway databases

ReactomeiR-GGA-901042. Calnexin/calreticulin cycle.
R-GGA-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Miscellaneous databases

PROiQ8JG64.

Family and domain databases

Gene3Di3.40.30.10. 3 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 1 hit.
PROSITEiPS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The amino-acid sequence of chicken homologue of ERp57."
    Gao B.
    Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: White leghorn.
    Tissue: Liver.

Entry informationi

Entry nameiPDIA3_CHICK
AccessioniPrimary (citable) accession number: Q8JG64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: October 1, 2002
Last modified: June 8, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.