Reviewed, UniProtKB/Swiss-Prot Q8JG64 (PDIA3_CHICK)

Names and origin

Protein names

Recommended name:
    Protein disulfide-isomerase A3
    EC=5.3.4.1
Alternative name(s):
    ERp57
    Glucose-regulated thiol oxidoreductase 58 kDa protein

Gene names

Name:	PDIA3
Synonyms:	ERP57, GRP58

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Catalyzes the rearrangement of -S-S- bonds in proteins.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 2 thioredoxin domains.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Redox-active center Repeat Signal
Molecular function	Isomerase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein disulfide isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 505	481	Protein disulfide-isomerase A3		PRO_5000088911
Regions
	Domain	25 – 131	107	Thioredoxin 1
	Domain	341 – 483	143	Thioredoxin 2
	Motif	502 – 505	4	Prevents secretion from ER By similarity
Sites
	Active site	55	1	Nucleophile By similarity
	Active site	58	1	Nucleophile By similarity
	Active site	404	1	Nucleophile By similarity
	Active site	407	1	Nucleophile By similarity
	Site	56	1	Contributes to redox potential value By similarity
	Site	57	1	Contributes to redox potential value By similarity
	Site	117	1	Lowers pKa of C-terminal Cys of first active site By similarity
	Site	405	1	Contributes to redox potential value By similarity
	Site	406	1	Contributes to redox potential value By similarity
	Site	469	1	Lowers pKa of C-terminal Cys of second active site By similarity
Amino acid modifications
	Disulfide bond	55 ↔ 58		Redox-active By similarity
	Disulfide bond	83 ↔ 90		By similarity
	Disulfide bond	404 ↔ 407		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8JG64-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 996509BBDC2D000A	FASTA	505	56,182
	10 20 30 40 50 60 MSVPRPSRAA LLLLVPLLAL SAGASDVVEL SDADFESGLA ERPGLVLVEF FAPWCGHCKR 70 80 90 100 110 120 LAPEYEAAAT RLKGIVPLVK VDCTANSNTC NKYGVSGYPT LKIFRDGEES GTYDGPRTAD 130 140 150 160 170 180 GIVSHLKKQA GPASVALSSV ADFEKFIGDK DASVVGFFRD ASGDAYSEFM KAANNLRDNY 190 200 210 220 230 240 RFAHTSEEQL VQKYEEDGEG VVLYRPSRLA NKFEDSTVKY TEDKITSAKI KKFIQENIFG 250 260 270 280 290 300 ICPHMTEDNK DLIQGKDLLV AYYDVDYEKN AKGSNYWRNR VMMIAKKFLD AGHKLSFAVA 310 320 330 340 350 360 SRKTFGHELS EFGLDNSVGE APVVAIRTAK GDKFVMQEEF SRDGKALERF LQDYFDGNLK 370 380 390 400 410 420 KYLKSEPVPE NNDGPVKVVV AENFDEIVNA EDKDVLIEFY APWCGHCKNL EPKYKELGEK 430 440 450 460 470 480 LSKDPNIVIA KMDATANDVP SPYEVRGFPT IYFAPAGKKQ SPKKYEGGRE VSDFISYLKR 490 500 EATSTPVLQE EDKAKKSKKK AKEDL

References

[1]	"The amino-acid sequence of chicken homologue of ERp57." Gao B. Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: White leghorn. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY122886 mRNA. Translation: AAM82759.1.
IPI	IPI00575271.
RefSeq	NP_989441.1.
UniGene	Gga.3802
3D structure databases
HSSP	HSSP built from PDB template 1MEK based on UniProtKB P07237.
SMR	Q8JG64. Positions 25-135.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8JG64.
Proteomic databases
PRIDE	Q8JG64.
Genome annotation databases
Ensembl	ENSGALT00000013589; ENSGALP00000013574; ENSGALG00000008348; Gallus gallus. [Genome view]
GeneID	373899.
KEGG	gga:373899.
Organism-specific databases
CTD	373899.
Phylogenomic databases
HOGENOM	Q8JG64.
HOVERGEN	Q8JG64.
OMA	EGITLFR.
Enzyme and pathway databases
BRENDA	5.3.4.1. 4.
Family and domain databases
InterPro	IPR005788. Disulphide_isomerase. IPR005792. Prot_disulphide_isomerase. IPR017936. Thioredoxin-like. IPR006662. Thioredoxin-like_subdom. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 2 hits.
Pfam	PF00085. Thioredoxin. 2 hits. [Graphical view]
PRINTS	PR00421. THIOREDOXIN.
TIGRFAMs	TIGR01130. ER_PDI_fam. 1 hit. TIGR01126. pdi_dom. 2 hits.
PROSITE	PS00194. THIOREDOXIN_1. 2 hits. PS51352. THIOREDOXIN_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDIA3_CHICK

Accession

Primary (citable) accession number: Q8JG64

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	October 1, 2002
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)