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Q8JFZ2

- GSTP1_XENLA

UniProt

Q8JFZ2 - GSTP1_XENLA

Protein

Glutathione S-transferase P 1

Gene

gstp1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Highly active towards 1-chloro-2,4-dinitrobenzene and organic isothiocyanates, but shows no detectable activity towards 1,2-dichloro-4-nitrobenzene, p-nitrobenzylchloride, trans-4-phenyl-3-buten-2-one (tPBO) and ethacrynic acid. May be associated with cellular proliferation.1 Publication1 Publication

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei8 – 81GlutathioneBy similarity
    Binding sitei14 – 141GlutathioneBy similarity
    Binding sitei39 – 391GlutathioneBy similarity
    Binding sitei47 – 471GlutathioneBy similarity

    GO - Molecular functioni

    1. glutathione transferase activity Source: UniProtKB

    GO - Biological processi

    1. glutathione metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Transferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase P 1 (EC:2.5.1.18)
    Alternative name(s):
    GST class-pi
    Short name:
    GST-Pi
    XlGSTP1-1
    Gene namesi
    Name:gstp1
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-5846294. gstp1.

    Subcellular locationi

    Cytoplasm By similarity. Mitochondrion By similarity. Nucleus By similarity
    Note: The 83 N-terminal amino acids function as un uncleaved transit peptide, and arginine residues within it are crucial for mitochondrial localization.By similarity

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi111 – 1111F → Y: Reduction in activity and altered substrate specificity; inactive towards 4-nitroquinoline-1-oxide, active towards ethacrynic acid and tPBO. 1 Publication
    Mutagenesisi208 – 2081P → G or H: Reduction in activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 212212Glutathione S-transferase P 1PRO_0000185910Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed only in embryos. Not expressed in liver, lung, heart, kidney and ovary.1 Publication

    Developmental stagei

    Detected in embryos at stages 11-15, but not detected in unfertilized eggs.1 Publication

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ8JFZ2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 8382GST N-terminalAdd
    BLAST
    Domaini85 – 206122GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni54 – 552Glutathione bindingBy similarity
    Regioni67 – 682Glutathione bindingBy similarity

    Sequence similaritiesi

    Belongs to the GST superfamily. Pi family.Sequence Analysis
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    HOVERGENiHBG108324.
    KOiK00799.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR003082. GST_pi.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view]
    PRINTSiPR01268. GSTRNSFRASEP.
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8JFZ2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPGYVLTYFP VRGRAEPIRL LLADQGISWK EDEVQIPDWF SGKDARKKEA    50
    VFGQLPQFQD GDYVLYQSNS ILRYLGNKHG LTGANDEERG HIDMVNDGVE 100
    DLRQKYGRLI FFEYETGKDK YLKELPSQLD FFERILSKNA NGSKFVVGQK 150
    ISFADYNLLD ILQCHLDLCS KSLSAYPLLT AYVERLVARP KISEYLKSDA 200
    RNKRPITPKH KK 212
    Length:212
    Mass (Da):24,428
    Last modified:October 1, 2002 - v1
    Checksum:iE5BF0166C08CC5BA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti203 – 2031K → R in AAH68854. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ489617 mRNA. Translation: CAD33920.1.
    BC068854 mRNA. Translation: AAH68854.1.
    RefSeqiNP_001082252.1. NM_001088783.1.
    UniGeneiXl.54920.

    Genome annotation databases

    GeneIDi398321.
    KEGGixla:398321.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ489617 mRNA. Translation: CAD33920.1 .
    BC068854 mRNA. Translation: AAH68854.1 .
    RefSeqi NP_001082252.1. NM_001088783.1.
    UniGenei Xl.54920.

    3D structure databases

    ProteinModelPortali Q8JFZ2.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 398321.
    KEGGi xla:398321.

    Organism-specific databases

    CTDi 2950.
    Xenbasei XB-GENE-5846294. gstp1.

    Phylogenomic databases

    HOVERGENi HBG108324.
    KOi K00799.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR003082. GST_pi.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view ]
    PRINTSi PR01268. GSTRNSFRASEP.
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel amphibian Pi-class glutathione transferase isoenzyme from Xenopus laevis: importance of phenylalanine 111 in the H-site."
      De Luca A., Favaloro B., Carletti E., Sacchetta P., Di Ilio C.
      Biochem. J. 373:539-545(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME ACTIVITY, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF PHE-111 AND PRO-208.
      Tissue: Liver tumor1 Publication.
    2. NIH - Xenopus Gene Collection (XGC) project
      Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 18-212.
      Tissue: EmbryoImported.

    Entry informationi

    Entry nameiGSTP1_XENLA
    AccessioniPrimary (citable) accession number: Q8JFZ2
    Secondary accession number(s): Q6NTV0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3