Q8J308 (ALF1_PYRKO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fructose-bisphosphate aldolase class 1
EC=4.1.2.13

Alternative name(s):
Fructose-bisphosphate aldolase class I
Short name=FBP aldolase
Tk-fba

Gene names

Name:	fba
Ordered Locus Names:	TK0989

Organism

Pyrococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Thermococcus kodakaraensis (strain KOD1)) [Reference proteome] [HAMAP]

Taxonomic identifier

69014 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus › Thermococcus kodakarensis

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Enzyme regulation	Activated by citrate By similarity.
Subunit structure	Homooctamer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the DeoC/FbaB aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	Fructose-bisphosphate aldolase class 1		PRO_0000138951
Sites
	Active site	191	1	Schiff-base intermediate with dihydroxyacetone-P By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8J308 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 9A95B90F50324B79	FASTA	281	31,240
	10 20 30 40 50 60 MDAYQSVGIR RRLKRFFRRD GRALIFAMDH GFEHGPTDFE PVWEHVNPRI IIRKVVRAGV 70 80 90 100 110 120 DGVMMLPGIV RMAGDELKPD TGLMIKLTSK TELRPKEEQL LQSQLGYVED AIKLGADAIA 130 140 150 160 170 180 ATVYWGSPQE DVMMRQFAEI ASYAHDLGFP VVQFAYPRGP YINEKYGKKE DYRVVMYGAR 190 200 210 220 230 240 AAAETGADMI KTYWTGSKET FAKVVDAAAG VPVLLSGGAK TDNPVDFLKV VWDVIEAGGA 250 260 270 280 GAVVGRNIFQ RENPEPMIKA LIRVIHRNED PEEAAKAEGL I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Fructose-1,6-bisphosphate aldolase from Thermococcus kodakaraensis KOD1." Imanaka H., Fukui T., Atomi H., Imanaka T. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC BAA-918 / JCM 12380 / KOD1.
[2]	"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T. Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB083709 Genomic DNA. Translation: BAC21177.1. AP006878 Genomic DNA. Translation: BAD85178.1.
RefSeq	YP_183402.1. NC_006624.1.
3D structure databases
ProteinModelPortal	Q8J308.
ModBase	Search...
Protein-protein interaction databases
STRING	69014.TK0989.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAD85178; BAD85178; TK0989.
GeneID	3233691.
KEGG	tko:TK0989.
Phylogenomic databases
eggNOG	COG1830.
HOGENOM	HOG000224790.
KO	K11645.
OMA	RVIHRNE.
ProtClustDB	PRK07226.
Enzyme and pathway databases
SABIO-RK	Q8J308.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR002915. DeoC/FbaB/lacD_aldolase. [Graphical view]
Pfam	PF01791. DeoC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALF1_PYRKO

Accession

Primary (citable) accession number: Q8J308

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program