Reviewed, UniProtKB/Swiss-Prot Q8J308 (ALF1_PYRKO)

Names and origin

Protein names

Recommended name:
    Fructose-bisphosphate aldolase class 1
    EC=4.1.2.13
Alternative name(s):
    Fructose-biphosphate aldolase class I
      Short name=FBP aldolase
    Tk-fba

Gene names

Name:	fba
Ordered Locus Names:	TK0989

Organism

Pyrococcus kodakaraensis (Thermococcus kodakaraensis) [Complete proteome] [HAMAP]

Taxonomic identifier

311400 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Enzyme regulation	Activated by citrate By similarity.
Subunit structure	Homooctamer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the deoC/fbaB aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	Fructose-bisphosphate aldolase class 1		PRO_0000138951
Sites
	Active site	191	1	Schiff-base intermediate with dihydroxyacetone-P By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8J308-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 9A95B90F50324B79	FASTA	281	31,240
	10 20 30 40 50 60 MDAYQSVGIR RRLKRFFRRD GRALIFAMDH GFEHGPTDFE PVWEHVNPRI IIRKVVRAGV 70 80 90 100 110 120 DGVMMLPGIV RMAGDELKPD TGLMIKLTSK TELRPKEEQL LQSQLGYVED AIKLGADAIA 130 140 150 160 170 180 ATVYWGSPQE DVMMRQFAEI ASYAHDLGFP VVQFAYPRGP YINEKYGKKE DYRVVMYGAR 190 200 210 220 230 240 AAAETGADMI KTYWTGSKET FAKVVDAAAG VPVLLSGGAK TDNPVDFLKV VWDVIEAGGA 250 260 270 280 GAVVGRNIFQ RENPEPMIKA LIRVIHRNED PEEAAKAEGL I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Fructose-1,6-bisphosphate aldolase from Thermococcus kodakaraensis KOD1." Imanaka H., Fukui T., Atomi H., Imanaka T. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: KOD1.
[2]	"Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes." Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T. Genome Res. 15:352-363(2005) [PubMed: 15710748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KOD1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB083709 Genomic DNA. Translation: BAC21177.1. AP006878 Genomic DNA. Translation: BAD85178.1.
RefSeq	YP_183402.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8J308.
Genome annotation databases
GeneID	3233691.
GenomeReviews	Gene locus TK0989 in contig AP006878_GR.
KEGG	tko:TK0989.
NMPDR	fig|69014.3.peg.631.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05005.
HOGENOM	HBG553580.
OMA	VRHGHRG.
Enzyme and pathway databases
BioCyc	TKOD69014:TK0989-MONOMER.
BRENDA	4.1.2.13. 192130.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR002915. DeoC/AroFGH_arch. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01791. DeoC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALF1_PYRKO

Accession

Primary (citable) accession number: Q8J308

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	March 1, 2003
Last modified:	January 19, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)