Q8J232 (ACEA_MAGO7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate lyase
Short name=ICL
Short name=Isocitrase
Short name=Isocitratase
EC=4.1.3.1

Gene names

Name:	ICL1
ORF Names:	MGG_04895

Organism

Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae) [Complete proteome]

Taxonomic identifier

242507 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Sordariomycetidae › Magnaporthales › Magnaporthaceae › Magnaporthe › Magnaporthe oryzae

Protein attributes

Sequence length	547 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Isocitrate = succinate + glyoxylate.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.
Subunit structure	Homotetramer By similarity.
Subcellular location	Peroxisome By similarity.
Sequence similarities	Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Peroxisome
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	isocitrate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 547	547	Isocitrate lyase		PRO_0000068793
Sites
	Active site	215	1	By similarity
Natural variations
	Natural variant	428	1	Q → L in strain: Y34.

Sequences

	Sequence		Length	Mass (Da)
	Q8J232 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 0BF3F9921E04D1D0	FASTA	547	61,001
	10 20 30 40 50 60 MASKNMVNPA VEPSMEDDLF AREVAEVKQW WSDPRWRYTK RPFTAEQIVS KRGNLKIEYP 70 80 90 100 110 120 SNAQSKKLWK ILEGRFQKRD ASYTYGCLEP TMVTQMAKYL DTVYVSGWQS SSTASSSDEP 130 140 150 160 170 180 GPDLADYPYT TVPNKVSHLF MAQLFHDRKQ RHERLSAPKS ERSKLQNIDY LRPIIADADT 190 200 210 220 230 240 GHGGLTAVMK LTKLFIEKGA AGIHIEDQAP GTKKCGHMAG KVLVPISEHI NRLVAIRAQA 250 260 270 280 290 300 DIMGVDLLAI ARTDAEAATL ITTSIDPRDH AFILGCTNPS LQPLADLMNT AEQSGKTGDQ 310 320 330 340 350 360 LQAIEDEWMA KANLKRFDDA VVDVINSSSS IRNPKDVAAK YLQAAKGKSN REARAIASSL 370 380 390 400 410 420 GVPEIFFDWD SPRTREGYFR IKGGCDCAIN RAIAYAPYAD AIWMESKLPD YEQAKEFAEG 430 440 450 460 470 480 VHAVYPEQKL AYNLSPSFNW KTAMPRDEQE TYIRRLAGLG YCWQFITLAG LHTTALISDR 490 500 510 520 530 540 FARAYSEVGM RAYGELVQEP EMELGVDVVK HQKWSGATYV DELQKMVTGG VSSTAAMGKG VTEDQFH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The glyoxylate cycle is required for temporal regulation of virulence by the plant pathogenic fungus Magnaporthe grisea." Wang Z.Y., Thornton C.R., Kershaw M.J., Debao L., Talbot N.J. Mol. Microbiol. 47:1601-1612(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Guyane 11.
[2]	Dong H.T., Peng Y.L., Chen B.S., Li Y.Z., Li D.B. Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Y34.
[3]	"The genome sequence of the rice blast fungus Magnaporthe grisea." Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K., Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D., Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S., Soanes D.M. , Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M., Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E., Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W. Nature 434:980-986(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 70-15 / ATCC MYA-4617 / FGSC 8958.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF540383 Genomic DNA. Translation: AAN28719.1. AY849617 mRNA. Translation: AAX07638.1. CM001233 Genomic DNA. Translation: EHA52574.1.
RefSeq	XP_003712381.1. XM_003712333.1.
3D structure databases
ProteinModelPortal	Q8J232.
SMR	Q8J232. Positions 16-530.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	MGG_04895T0; MGG_04895T0; MGG_04895.
GeneID	2675603.
KEGG	mgr:MGG_04895.
Phylogenomic databases
eggNOG	COG2224.
KO	K01637.
OrthoDB	EOG4P5PJP.
Enzyme and pathway databases
UniPathway	UPA00703; UER00719.
Family and domain databases
Gene3D	3.20.20.60. 2 hits.
InterPro	IPR006254. Isocitrate_lyase. IPR000918. Isocitrate_lyase/Pmutase. IPR018523. Isocitrate_lyase_ph_CS. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
PANTHER	PTHR21631:SF3. PTHR21631:SF3. 1 hit.
Pfam	PF00463. ICL. 1 hit. [Graphical view]
PIRSF	PIRSF001362. Isocit_lyase. 1 hit.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01346. isocit_lyase. 1 hit.
PROSITE	PS00161. ISOCITRATE_LYASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL1075264.

Entry information

Entry name

ACEA_MAGO7

Accession

Primary (citable) accession number: Q8J232
Secondary accession number(s): A4QU17, G4N2R9, Q5EN15

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	March 1, 2003
Last modified:	April 3, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program