Reviewed, UniProtKB/Swiss-Prot Q8J0U0 (TRXB_PNEJI)

Names and origin

Protein names

Recommended name:
    Thioredoxin reductase
    EC=1.8.1.9

Gene names

Name:

TRR1

Organism

Pneumocystis jiroveci

Taxonomic identifier

42068 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Pneumocystidomycetes › Pneumocystidaceae › Pneumocystis

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Component of the thioredoxin-thioredoxin reductase system which may be involved in biosynthesis of penicillins and cephalosporins and may be important in determining the thiol-disulfide redox balance.
Catalytic activity	Thioredoxin + NADP+ = thioredoxin disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW removal of superoxide radicals Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro thioredoxin-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Thioredoxin reductase		PRO_0000166767
Regions
	Nucleotide binding	32 – 44	13	FAD By similarity
	Nucleotide binding	286 – 295	10	FAD By similarity
Amino acid modifications
	Disulfide bond	140 ↔ 143		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8J0U0-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 6A5D230F87BF5777	FASTA	327	35,608
	10 20 30 40 50 60 MHSKVIIIGS GPAGHTAAIY LARAELKPVL YEGMLANGIA AGGQLTTTTD VENYPGFPDG 70 80 90 100 110 120 IMGPLLMEKF REQSVKYGTC IITETVSKLD LSKRPFKYWC ESDENTCHTA DVVVMATGAY 130 140 150 160 170 180 ARRLHIPGEE TYWQRGISAC AVCDGAAPIF RGKCLSVVGG GDSAAEESLF LTRYATKVYL 190 200 210 220 230 240 LVRRDKLRAS AVMAKRLLNH PKIEVIWNTV VLRSLGDGHL LNRLEIKNVK TQVVSELHVS 250 260 270 280 290 300 GLFYAIGHEP ATALVRGQVE CDDNGYIITK NGGPETNIKG FFAAGDVQDK KWRQAVTSAG 310 320 SGCMAGLAAE RLLAEEEEMK NIEKNKK

References

[1]	"Characterization of thioredoxin reductase genes (trr1) from Pneumocystis carinii and Pneumocystis jiroveci." Kutty G.K., Huang S.N., Kovacs J.A. Gene 310:175-183(2003) [PubMed: 12801645] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY130996 Genomic DNA. Translation: AAN12366.1. AF532986 mRNA. Translation: AAP72145.1.
3D structure databases
HSSP	HSSP built from PDB template 1VDC based on UniProtKB Q39243.
ModBase	Search...
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR008255. Pyr_nucl-diS_OxRdtase_2_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR000103. Pyridine_nuc-diS_OxRdtase_2. IPR005982. Thioredox_Rdtase. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00469. PNDRDTASEII.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01292. TRX_reduct. 1 hit.
PROSITE	PS00573. PYRIDINE_REDOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRXB_PNEJI

Accession

Primary (citable) accession number: Q8J0U0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 9, 2004
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)