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Q8J0N6

- ALF2_PARBA

UniProt

Q8J0N6 - ALF2_PARBA

Protein

Fructose-bisphosphate aldolase 2

Gene

FBA2

Organism
Paracoccidioides lutzii (strain ATCC MYA-826 / Pb01) (Paracoccidioides brasiliensis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 2 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

    Catalytic activityi

    D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

    Cofactori

    Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei61 – 611Glyceraldehyde 3-phosphateBy similarity
    Active sitei109 – 1091Proton donorBy similarity
    Metal bindingi110 – 1101Zinc 1; catalyticBy similarity
    Metal bindingi144 – 1441Zinc 2By similarity
    Metal bindingi174 – 1741Zinc 2By similarity
    Metal bindingi226 – 2261Zinc 1; catalyticBy similarity
    Binding sitei227 – 2271Dihydroxyacetone phosphate; via amide nitrogenBy similarity
    Metal bindingi264 – 2641Zinc 1; catalyticBy similarity

    GO - Molecular functioni

    1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00183.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructose-bisphosphate aldolase 2 (EC:4.1.2.13)
    Short name:
    FBP aldolase 2
    Short name:
    FBPA 2
    Alternative name(s):
    Fructose-1,6-bisphosphate aldolase 2
    Gene namesi
    Name:FBA2
    OrganismiParacoccidioides lutzii (strain ATCC MYA-826 / Pb01) (Paracoccidioides brasiliensis)
    Taxonomic identifieri502779 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeOnygenalesmitosporic OnygenalesParacoccidioides

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 363362Fructose-bisphosphate aldolase 2PRO_0000178760Add
    BLAST

    Expressioni

    Inductioni

    Only detected in the mycelia phase, but not in yeast cells.1 Publication

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ8J0N6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni265 – 2673Dihydroxyacetone phosphate bindingBy similarity

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR006411. Fruct_bisP_bact.
    IPR000771. Ketose_bisP_aldolase_II.
    [Graphical view]
    PfamiPF01116. F_bP_aldolase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
    TIGRFAMsiTIGR00167. cbbA. 1 hit.
    TIGR01520. FruBisAldo_II_A. 1 hit.
    PROSITEiPS00602. ALDOLASE_CLASS_II_1. 2 hits.
    PS00806. ALDOLASE_CLASS_II_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8J0N6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGVKDLLSRK TGVIVGDDVL RLFQHAQENV FAIPAIMVTS SSTGSALEAA    50
    RDKSPIVLQT SNGAAYFAGK GKGVSNDGQS VAGSIAIAAA HYIRSQAPAY 100
    GIPVVLHTDH CAKKLLPWLD GMLDADECYS KLHNEPLFSS HMIDLSEESV 150
    EWNIETTAKY LKRAAPMKQW LEMEIGITGG EEDGVNNESV DNNSLYTQPE 200
    DIYTIYKTLS AISPYFSIAA GFGNVHGVYR GDIALRPLLR HLHNAKYDEQ 250
    LKCLQDPLGF FVFHGGSGSS HQPATSITSE FAAKGRVDLE KVAYLPGTSS 300
    LVIAPKDYLM SPYGIPVVLH TDHCKYFDPR VQIREGLKHM SARVQEAKDD 350
    FNDSNQAKKL LPW 363
    Length:363
    Mass (Da):39,770
    Last modified:October 1, 2003 - v2
    Checksum:i929C731386F46D76
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY172327 mRNA. Translation: AAN85569.2.
    AY581212 Genomic DNA. Translation: AAS99115.2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY172327 mRNA. Translation: AAN85569.2 .
    AY581212 Genomic DNA. Translation: AAS99115.2 .

    3D structure databases

    ProteinModelPortali Q8J0N6.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00183 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR006411. Fruct_bisP_bact.
    IPR000771. Ketose_bisP_aldolase_II.
    [Graphical view ]
    Pfami PF01116. F_bP_aldolase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001359. F_bP_aldolase_II. 1 hit.
    TIGRFAMsi TIGR00167. cbbA. 1 hit.
    TIGR01520. FruBisAldo_II_A. 1 hit.
    PROSITEi PS00602. ALDOLASE_CLASS_II_1. 2 hits.
    PS00806. ALDOLASE_CLASS_II_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Paracoccidioides brasiliensis presents two different cDNAs encoding homologues of the fructose 1,6-biphosphate aldolase: protein isolation, cloning of the cDNAs and genes, structural, phylogenetic, and expression analysis."
      Carneiro L.C., de Faria F.P., Felipe M.S.S., Pereira M., de Almeida Soares C.M.
      Fungal Genet. Biol. 42:51-60(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], INDUCTION.
      Strain: ATCC MYA-826 / Pb01.

    Entry informationi

    Entry nameiALF2_PARBA
    AccessioniPrimary (citable) accession number: Q8J0N6
    Secondary accession number(s): Q6PT81
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2004
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 52 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Caution

    The DNA coding for this protein is not found in the complete genome of strain ATCC MYA-826 / Pb01.Curated

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3