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Protein

Endo-1,6-beta-D-glucanase BGN16.3

Gene
N/A
Organism
Trichoderma harzianum (Hypocrea lixii)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has highest activity on the linear beta-1,6-glucan pustulan. Lower activity against yeast glucan and laminarin (beta-1,3-glucans with beta-1,6-branches). No activity on colloidal chitin, pachyman, starch, cellulose, nigeran, dextran or gentobiose.1 Publication

Catalytic activityi

Random hydrolysis of (1->6)-linkages in (1->6)-beta-D-glucans.1 Publication

Kineticsi

    1. Vmax=390 µmol/min/mg enzyme1 Publication

    pH dependencei

    Optimum pH is 5.0. At least 20% of maximum activity is retained between pH 4.0 and 7.0.1 Publication

    Temperature dependencei

    Optimum temperature is 50 degrees Celsius. After 30 min incubation at 50 degrees Celsius in the absence of substrate 50% of activity is lost.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei243 – 2431Proton donorBy similarity
    Active sitei339 – 3391NucleophileBy similarity

    GO - Molecular functioni

    GO - Biological processi

    • polysaccharide metabolic process Source: UniProtKB
    • sphingolipid metabolic process Source: InterPro
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    SABIO-RKQ8J0I9.

    Protein family/group databases

    CAZyiGH30. Glycoside Hydrolase Family 30.
    mycoCLAPiBGN30P_TRIHA.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endo-1,6-beta-D-glucanase BGN16.3 (EC:3.2.1.75)
    Alternative name(s):
    Beta-1,6-glucanase BGN16.3
    Glucan endo-1,6-beta-glucosidase BGN16.3
    OrganismiTrichoderma harzianum (Hypocrea lixii)
    Taxonomic identifieri5544 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesHypocreaceaeTrichoderma

    Subcellular locationi

    GO - Cellular componenti

    • extracellular region Source: UniProtKB
    • extracellular space Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 28281 PublicationAdd
    BLAST
    Chaini29 – 490462Endo-1,6-beta-D-glucanase BGN16.31 PublicationPRO_0000042853Add
    BLAST

    Expressioni

    Inductioni

    By fungal cell walls.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ8J0I9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi195 – 2028Poly-AsnSequence Analysis

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 30 family.Sequence Analysis

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR013780. Glyco_hydro_13_b.
    IPR001139. Glyco_hydro_30.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR11069. PTHR11069. 1 hit.
    PfamiPF02055. Glyco_hydro_30. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8J0I9-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRYALIASML GQAAISVAMP SEPAHSPRAA GAQAYASNQA GNYKLTSIAA
    60 70 80 90 100
    PVQGNGSPGP STWNLSIDDT SSGYKQKIVG FGAAVTDATV SAFNELSAST
    110 120 130 140 150
    LSQLLDELMT GAGASFSLMR HTIGASDLSG DPAYTYDDNG GNADPGMTGF
    160 170 180 190 200
    NLGDRGTAMA TMLAQMKGLN SNLQIFGSPW SAPGWMKLNN AIDGNTNNNN
    210 220 230 240 250
    LNDGYLTNNG AQYSAAFAQY FVKYIQAFES HGATINAITL QNEPLNSQAG
    260 270 280 290 300
    YPTMYMFSYE QGDLIQNYVA PALKAAGLST KIWAYDHNTD QPDFPEQVMG
    310 320 330 340 350
    IAADDVSAVA WHCYATNLDW TVLTNFHNSY PNTDQYMTEC WTPSTGAWNQ
    360 370 380 390 400
    AASFTMGPLQ NWARGVAAWT LGTTAQDGPH LSSGGCGTCT GLVTINNGQY
    410 420 430 440 450
    TFQTAYYMMA QFSKFMPVGA TVLSGTGSYT YSGSGGVQSV ASLNPDGTRT
    460 470 480 490
    VVIENTFGND IYIHLSTSSG QEWSGNVPTN SVTTWVLPAV
    Length:490
    Mass (Da):51,890
    Last modified:March 1, 2003 - v1
    Checksum:i5900F8B8D4B6E9F7
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ243823 mRNA. Translation: CAC80492.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ243823 mRNA. Translation: CAC80492.1.

    3D structure databases

    ProteinModelPortaliQ8J0I9.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein family/group databases

    CAZyiGH30. Glycoside Hydrolase Family 30.
    mycoCLAPiBGN30P_TRIHA.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Enzyme and pathway databases

    SABIO-RKQ8J0I9.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR013780. Glyco_hydro_13_b.
    IPR001139. Glyco_hydro_30.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR11069. PTHR11069. 1 hit.
    PfamiPF02055. Glyco_hydro_30. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    1. Montero M.
      Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ATCC 48131 / CBS 354.33 / CECT 2413 / VTT D-80150.
    2. "BGN16.3, a novel acidic beta-1,6-glucanase from mycoparasitic fungus Trichoderma harzianum CECT 2413."
      Montero M., Sanz L., Rey M., Monte E., Llobell A.
      FEBS J. 272:3441-3448(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 29-42 AND 168-180, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, INDUCTION.
      Strain: ATCC 48131 / CBS 354.33 / CECT 2413 / VTT D-80150.

    Entry informationi

    Entry nameiGUN16_TRIHA
    AccessioniPrimary (citable) accession number: Q8J0I9
    Secondary accession number(s): P84511
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: March 1, 2003
    Last modified: January 7, 2015
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.