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Q8J0I9

- GUN16_TRIHA

UniProt

Q8J0I9 - GUN16_TRIHA

Protein

Endo-1,6-beta-D-glucanase BGN16.3

Gene
N/A
Organism
Trichoderma harzianum (Hypocrea lixii)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Has highest activity on the linear beta-1,6-glucan pustulan. Lower activity against yeast glucan and laminarin (beta-1,3-glucans with beta-1,6-branches). No activity on colloidal chitin, pachyman, starch, cellulose, nigeran, dextran or gentobiose.1 Publication

    Catalytic activityi

    Random hydrolysis of (1->6)-linkages in (1->6)-beta-D-glucans.1 Publication

    Kineticsi

      Vmax=390 µmol/min/mg enzyme1 Publication

      pH dependencei

      Optimum pH is 5.0. At least 20% of maximum activity is retained between pH 4.0 and 7.0.1 Publication

      Temperature dependencei

      Optimum temperature is 50 degrees Celsius. After 30 min incubation at 50 degrees Celsius in the absence of substrate 50% of activity is lost.1 Publication

      Sites

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Active sitei243 – 2431Proton donorBy similarity
      Active sitei339 – 3391NucleophileBy similarity

      GO - Molecular functioni

      1. glucan endo-1,6-beta-glucosidase activity Source: UniProtKB-EC
      2. glucosylceramidase activity Source: InterPro
      3. hydrolase activity, acting on glycosyl bonds Source: UniProtKB

      GO - Biological processi

      1. polysaccharide metabolic process Source: UniProtKB
      2. sphingolipid metabolic process Source: InterPro

      Keywords - Molecular functioni

      Glycosidase, Hydrolase

      Enzyme and pathway databases

      SABIO-RKQ8J0I9.

      Protein family/group databases

      CAZyiGH30. Glycoside Hydrolase Family 30.
      mycoCLAPiBGN30P_TRIHA.

      Names & Taxonomyi

      Protein namesi
      Recommended name:
      Endo-1,6-beta-D-glucanase BGN16.3 (EC:3.2.1.75)
      Alternative name(s):
      Beta-1,6-glucanase BGN16.3
      Glucan endo-1,6-beta-glucosidase BGN16.3
      OrganismiTrichoderma harzianum (Hypocrea lixii)
      Taxonomic identifieri5544 [NCBI]
      Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesHypocreaceaeTrichoderma

      Subcellular locationi

      Secretedextracellular space 1 Publication

      GO - Cellular componenti

      1. extracellular region Source: UniProtKB
      2. extracellular space Source: UniProtKB-SubCell

      Keywords - Cellular componenti

      Secreted

      PTM / Processingi

      Molecule processing

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Signal peptidei1 – 28281 PublicationAdd
      BLAST
      Chaini29 – 490462Endo-1,6-beta-D-glucanase BGN16.31 PublicationPRO_0000042853Add
      BLAST

      Expressioni

      Inductioni

      By fungal cell walls.1 Publication

      Structurei

      3D structure databases

      ProteinModelPortaliQ8J0I9.
      ModBaseiSearch...
      MobiDBiSearch...

      Family & Domainsi

      Compositional bias

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Compositional biasi195 – 2028Poly-AsnSequence Analysis

      Sequence similaritiesi

      Belongs to the glycosyl hydrolase 30 family.Sequence Analysis

      Keywords - Domaini

      Signal

      Family and domain databases

      Gene3Di2.60.40.1180. 1 hit.
      3.20.20.80. 1 hit.
      InterProiIPR013780. Glyco_hydro_13_b.
      IPR001139. Glyco_hydro_30.
      IPR013781. Glyco_hydro_catalytic_dom.
      IPR017853. Glycoside_hydrolase_SF.
      [Graphical view]
      PANTHERiPTHR11069. PTHR11069. 1 hit.
      PfamiPF02055. Glyco_hydro_30. 1 hit.
      [Graphical view]
      SUPFAMiSSF51445. SSF51445. 1 hit.

      Sequencei

      Sequence statusi: Complete.

      Sequence processingi: The displayed sequence is further processed into a mature form.

      Q8J0I9-1 [UniParc]FASTAAdd to Basket

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      MRYALIASML GQAAISVAMP SEPAHSPRAA GAQAYASNQA GNYKLTSIAA    50
      PVQGNGSPGP STWNLSIDDT SSGYKQKIVG FGAAVTDATV SAFNELSAST 100
      LSQLLDELMT GAGASFSLMR HTIGASDLSG DPAYTYDDNG GNADPGMTGF 150
      NLGDRGTAMA TMLAQMKGLN SNLQIFGSPW SAPGWMKLNN AIDGNTNNNN 200
      LNDGYLTNNG AQYSAAFAQY FVKYIQAFES HGATINAITL QNEPLNSQAG 250
      YPTMYMFSYE QGDLIQNYVA PALKAAGLST KIWAYDHNTD QPDFPEQVMG 300
      IAADDVSAVA WHCYATNLDW TVLTNFHNSY PNTDQYMTEC WTPSTGAWNQ 350
      AASFTMGPLQ NWARGVAAWT LGTTAQDGPH LSSGGCGTCT GLVTINNGQY 400
      TFQTAYYMMA QFSKFMPVGA TVLSGTGSYT YSGSGGVQSV ASLNPDGTRT 450
      VVIENTFGND IYIHLSTSSG QEWSGNVPTN SVTTWVLPAV 490
      Length:490
      Mass (Da):51,890
      Last modified:March 1, 2003 - v1
      Checksum:i5900F8B8D4B6E9F7
      GO

      Sequence databases

      Select the link destinations:
      EMBL
      GenBank
      DDBJ
      Links Updated
      AJ243823 mRNA. Translation: CAC80492.1.

      Cross-referencesi

      Sequence databases

      Select the link destinations:
      EMBL
      GenBank
      DDBJ
      Links Updated
      AJ243823 mRNA. Translation: CAC80492.1 .

      3D structure databases

      ProteinModelPortali Q8J0I9.
      ModBasei Search...
      MobiDBi Search...

      Protein family/group databases

      CAZyi GH30. Glycoside Hydrolase Family 30.
      mycoCLAPi BGN30P_TRIHA.

      Protocols and materials databases

      Structural Biology Knowledgebase Search...

      Enzyme and pathway databases

      SABIO-RK Q8J0I9.

      Family and domain databases

      Gene3Di 2.60.40.1180. 1 hit.
      3.20.20.80. 1 hit.
      InterProi IPR013780. Glyco_hydro_13_b.
      IPR001139. Glyco_hydro_30.
      IPR013781. Glyco_hydro_catalytic_dom.
      IPR017853. Glycoside_hydrolase_SF.
      [Graphical view ]
      PANTHERi PTHR11069. PTHR11069. 1 hit.
      Pfami PF02055. Glyco_hydro_30. 1 hit.
      [Graphical view ]
      SUPFAMi SSF51445. SSF51445. 1 hit.
      ProtoNeti Search...

      Publicationsi

      1. Montero M.
        Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
        Cited for: NUCLEOTIDE SEQUENCE [MRNA].
        Strain: ATCC 48131 / CBS 354.33 / CECT 2413 / VTT D-80150.
      2. "BGN16.3, a novel acidic beta-1,6-glucanase from mycoparasitic fungus Trichoderma harzianum CECT 2413."
        Montero M., Sanz L., Rey M., Monte E., Llobell A.
        FEBS J. 272:3441-3448(2005) [PubMed] [Europe PMC] [Abstract]
        Cited for: PROTEIN SEQUENCE OF 29-42 AND 168-180, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, INDUCTION.
        Strain: ATCC 48131 / CBS 354.33 / CECT 2413 / VTT D-80150.

      Entry informationi

      Entry nameiGUN16_TRIHA
      AccessioniPrimary (citable) accession number: Q8J0I9
      Secondary accession number(s): P84511
      Entry historyi
      Integrated into UniProtKB/Swiss-Prot: November 22, 2005
      Last sequence update: March 1, 2003
      Last modified: October 1, 2014
      This is version 47 of the entry and version 1 of the sequence. [Complete history]
      Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programFungal Protein Annotation Program

      Miscellaneousi

      Keywords - Technical termi

      Direct protein sequencing

      Documents

      1. Glycosyl hydrolases
        Classification of glycosyl hydrolase families and list of entries
      2. SIMILARITY comments
        Index of protein domains and families

      External Data

      Dasty 3