SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8J0I9

- GUN16_TRIHA

UniProt

Q8J0I9 - GUN16_TRIHA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Endo-1,6-beta-D-glucanase BGN16.3
Gene
N/A
Organism
Trichoderma harzianum (Hypocrea lixii)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Has highest activity on the linear beta-1,6-glucan pustulan. Lower activity against yeast glucan and laminarin (beta-1,3-glucans with beta-1,6-branches). No activity on colloidal chitin, pachyman, starch, cellulose, nigeran, dextran or gentobiose.1 Publication

Catalytic activityi

Random hydrolysis of (1->6)-linkages in (1->6)-beta-D-glucans.1 Publication

Kineticsi

    Vmax=390 µmol/min/mg enzyme1 Publication

    pH dependencei

    Optimum pH is 5.0. At least 20% of maximum activity is retained between pH 4.0 and 7.0.1 Publication

    Temperature dependencei

    Optimum temperature is 50 degrees Celsius. After 30 min incubation at 50 degrees Celsius in the absence of substrate 50% of activity is lost.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei243 – 2431Proton donor By similarityBy similarity
    Active sitei339 – 3391Nucleophile By similarityBy similarity

    GO - Molecular functioni

    1. glucan endo-1,6-beta-glucosidase activity Source: UniProtKB-EC
    2. glucosylceramidase activity Source: InterPro
    3. hydrolase activity, acting on glycosyl bonds Source: UniProtKB

    GO - Biological processi

    1. polysaccharide metabolic process Source: UniProtKB
    2. sphingolipid metabolic process Source: InterPro
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    SABIO-RKQ8J0I9.

    Protein family/group databases

    CAZyiGH30. Glycoside Hydrolase Family 30.
    mycoCLAPiBGN30P_TRIHA.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endo-1,6-beta-D-glucanase BGN16.3 (EC:3.2.1.75)
    Alternative name(s):
    Beta-1,6-glucanase BGN16.3
    Glucan endo-1,6-beta-glucosidase BGN16.3
    OrganismiTrichoderma harzianum (Hypocrea lixii)
    Taxonomic identifieri5544 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesHypocreaceaeTrichoderma

    Subcellular locationi

    Secretedextracellular space 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB
    2. extracellular space Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 28281 Publication
    Add
    BLAST
    Chaini29 – 490462Endo-1,6-beta-D-glucanase BGN16.31 Publication
    PRO_0000042853Add
    BLAST

    Expressioni

    Inductioni

    By fungal cell walls.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ8J0I9.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi195 – 2028Poly-Asn

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR013780. Glyco_hydro_13_b.
    IPR001139. Glyco_hydro_30.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR11069. PTHR11069. 1 hit.
    PfamiPF02055. Glyco_hydro_30. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8J0I9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRYALIASML GQAAISVAMP SEPAHSPRAA GAQAYASNQA GNYKLTSIAA    50
    PVQGNGSPGP STWNLSIDDT SSGYKQKIVG FGAAVTDATV SAFNELSAST 100
    LSQLLDELMT GAGASFSLMR HTIGASDLSG DPAYTYDDNG GNADPGMTGF 150
    NLGDRGTAMA TMLAQMKGLN SNLQIFGSPW SAPGWMKLNN AIDGNTNNNN 200
    LNDGYLTNNG AQYSAAFAQY FVKYIQAFES HGATINAITL QNEPLNSQAG 250
    YPTMYMFSYE QGDLIQNYVA PALKAAGLST KIWAYDHNTD QPDFPEQVMG 300
    IAADDVSAVA WHCYATNLDW TVLTNFHNSY PNTDQYMTEC WTPSTGAWNQ 350
    AASFTMGPLQ NWARGVAAWT LGTTAQDGPH LSSGGCGTCT GLVTINNGQY 400
    TFQTAYYMMA QFSKFMPVGA TVLSGTGSYT YSGSGGVQSV ASLNPDGTRT 450
    VVIENTFGND IYIHLSTSSG QEWSGNVPTN SVTTWVLPAV 490
    Length:490
    Mass (Da):51,890
    Last modified:March 1, 2003 - v1
    Checksum:i5900F8B8D4B6E9F7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ243823 mRNA. Translation: CAC80492.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ243823 mRNA. Translation: CAC80492.1 .

    3D structure databases

    ProteinModelPortali Q8J0I9.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH30. Glycoside Hydrolase Family 30.
    mycoCLAPi BGN30P_TRIHA.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    SABIO-RK Q8J0I9.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR013780. Glyco_hydro_13_b.
    IPR001139. Glyco_hydro_30.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR11069. PTHR11069. 1 hit.
    Pfami PF02055. Glyco_hydro_30. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Montero M.
      Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ATCC 48131 / CBS 354.33 / CECT 2413 / VTT D-80150.
    2. "BGN16.3, a novel acidic beta-1,6-glucanase from mycoparasitic fungus Trichoderma harzianum CECT 2413."
      Montero M., Sanz L., Rey M., Monte E., Llobell A.
      FEBS J. 272:3441-3448(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 29-42 AND 168-180, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, INDUCTION.
      Strain: ATCC 48131 / CBS 354.33 / CECT 2413 / VTT D-80150.

    Entry informationi

    Entry nameiGUN16_TRIHA
    AccessioniPrimary (citable) accession number: Q8J0I9
    Secondary accession number(s): P84511
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: March 1, 2003
    Last modified: October 16, 2013
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi