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Q8J0I9 (GUN16_TRIHA) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endo-1,6-beta-D-glucanase BGN16.3

EC=3.2.1.75
Alternative name(s):
Beta-1,6-glucanase BGN16.3
Glucan endo-1,6-beta-glucosidase BGN16.3
OrganismTrichoderma harzianum (Hypocrea lixii)
Taxonomic identifier5544 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesHypocreaceaeTrichoderma

Protein attributes

Sequence length490 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has highest activity on the linear beta-1,6-glucan pustulan. Lower activity against yeast glucan and laminarin (beta-1,3-glucans with beta-1,6-branches). No activity on colloidal chitin, pachyman, starch, cellulose, nigeran, dextran or gentobiose. Ref.2

Catalytic activity

Random hydrolysis of (1->6)-linkages in (1->6)-beta-D-glucans. Ref.2

Subcellular location

Secretedextracellular space Ref.2.

Induction

By fungal cell walls. Ref.2

Sequence similarities

Belongs to the glycosyl hydrolase 30 family.

Biophysicochemical properties

Kinetic parameters:

Vmax=390 µmol/min/mg enzyme Ref.2

pH dependence:

Optimum pH is 5.0. At least 20% of maximum activity is retained between pH 4.0 and 7.0. Ref.2

Temperature dependence:

Optimum temperature is 50 degrees Celsius. After 30 min incubation at 50 degrees Celsius in the absence of substrate 50% of activity is lost. Ref.2

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Ref.2
Chain29 – 490462Endo-1,6-beta-D-glucanase BGN16.3 Ref.2
PRO_0000042853

Regions

Compositional bias195 – 2028Poly-Asn

Sites

Active site2431Proton donor By similarity UniProtKB P04062
Active site3391Nucleophile By similarity UniProtKB P04062

Sequences

Sequence LengthMass (Da)Tools
Q8J0I9 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 5900F8B8D4B6E9F7

FASTA49051,890
        10         20         30         40         50         60 
MRYALIASML GQAAISVAMP SEPAHSPRAA GAQAYASNQA GNYKLTSIAA PVQGNGSPGP 

        70         80         90        100        110        120 
STWNLSIDDT SSGYKQKIVG FGAAVTDATV SAFNELSAST LSQLLDELMT GAGASFSLMR 

       130        140        150        160        170        180 
HTIGASDLSG DPAYTYDDNG GNADPGMTGF NLGDRGTAMA TMLAQMKGLN SNLQIFGSPW 

       190        200        210        220        230        240 
SAPGWMKLNN AIDGNTNNNN LNDGYLTNNG AQYSAAFAQY FVKYIQAFES HGATINAITL 

       250        260        270        280        290        300 
QNEPLNSQAG YPTMYMFSYE QGDLIQNYVA PALKAAGLST KIWAYDHNTD QPDFPEQVMG 

       310        320        330        340        350        360 
IAADDVSAVA WHCYATNLDW TVLTNFHNSY PNTDQYMTEC WTPSTGAWNQ AASFTMGPLQ 

       370        380        390        400        410        420 
NWARGVAAWT LGTTAQDGPH LSSGGCGTCT GLVTINNGQY TFQTAYYMMA QFSKFMPVGA 

       430        440        450        460        470        480 
TVLSGTGSYT YSGSGGVQSV ASLNPDGTRT VVIENTFGND IYIHLSTSSG QEWSGNVPTN 

       490 
SVTTWVLPAV 

« Hide

References

[1]Montero M.
Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: ATCC 48131 / CBS 354.33 / CECT 2413 / VTT D-80150.
[2]"BGN16.3, a novel acidic beta-1,6-glucanase from mycoparasitic fungus Trichoderma harzianum CECT 2413."
Montero M., Sanz L., Rey M., Monte E., Llobell A.
FEBS J. 272:3441-3448(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 29-42 AND 168-180, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, INDUCTION.
Strain: ATCC 48131 / CBS 354.33 / CECT 2413 / VTT D-80150.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ243823 mRNA. Translation: CAC80492.1.

3D structure databases

ProteinModelPortalQ8J0I9.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH30. Glycoside Hydrolase Family 30.
mycoCLAPBGN30P_TRIHA.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKQ8J0I9.

Family and domain databases

Gene3D2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProIPR013780. Glyco_hydro_13_b.
IPR001139. Glyco_hydro_30.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR11069. PTHR11069. 1 hit.
PfamPF02055. Glyco_hydro_30. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGUN16_TRIHA
AccessionPrimary (citable) accession number: Q8J0I9
Secondary accession number(s): P84511
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: March 1, 2003
Last modified: October 16, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries