Reviewed, UniProtKB/Swiss-Prot Q8J0I9 (GUN16_TRIHA)

Names and origin

Protein names	Recommended name:     Endo-1,6-beta-D-glucanase BGN16.3     EC=3.2.1.75 Alternative name(s):     Beta-1,6-glucanase BGN16.3     Glucan endo-1,6-beta-glucosidase BGN16.3
Organism	Trichoderma harzianum (Hypocrea lixii)
Taxonomic identifier	5544 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Hypocreaceae › Hypocrea

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has highest activity on the linear beta-1,6-glucan pustulan. Lower activity against yeast glucan and laminarin (beta-1,3-glucans with beta-1,6-branches). No activity on colloidal chitin, pachyman, starch, cellulose, nigeran, dextran or gentobiose. Ref.2
Catalytic activity	Random hydrolysis of (1->6)-linkages in (1->6)-beta-D-glucans. Ref.2
Subcellular location	Secreted › extracellular space. Ref.2
Induction	By fungal cell walls. Ref.2
Sequence similarities	Belongs to the glycosyl hydrolase 30 family.
biophysicochemical properties	Kinetic parameters: Vmax=390 µmol/min/mg enzyme Ref.2 pH dependence: Optimum pH is 5.0. At least 20% of maximum activity is retained between pH 4.0 and 7.0. Ref.2 Temperature dependence: Optimum temperature is 50 degrees Celsius. After 30 min incubation at 50 degrees Celsius in the absence of substrate 50% of activity is lost. Ref.2

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	lysosome organization Inferred from electronic annotation. Source: InterPro polysaccharide metabolic process Ref.2 Inferred from direct assay. Source: UniProtKB sphingolipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell lysosome Inferred from electronic annotation. Source: InterPro
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro glucan endo-1,6-beta-glucosidase activity Inferred from electronic annotation. Source: EC glucosylceramidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Ref.2
	Chain	29 – 490	462	Endo-1,6-beta-D-glucanase BGN16.3 Ref.2		PRO_0000042853
Regions
	Compositional bias	195 – 202	8	Poly-Asn
Sites
	Active site	243	1	Proton donor By similarity UniProtKB P04062
	Active site	339	1	Nucleophile By similarity UniProtKB P04062

Sequences

	Sequence		Length	Mass (Da)
	Q8J0I9-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 5900F8B8D4B6E9F7	FASTA	490	51,890
	10 20 30 40 50 60 MRYALIASML GQAAISVAMP SEPAHSPRAA GAQAYASNQA GNYKLTSIAA PVQGNGSPGP 70 80 90 100 110 120 STWNLSIDDT SSGYKQKIVG FGAAVTDATV SAFNELSAST LSQLLDELMT GAGASFSLMR 130 140 150 160 170 180 HTIGASDLSG DPAYTYDDNG GNADPGMTGF NLGDRGTAMA TMLAQMKGLN SNLQIFGSPW 190 200 210 220 230 240 SAPGWMKLNN AIDGNTNNNN LNDGYLTNNG AQYSAAFAQY FVKYIQAFES HGATINAITL 250 260 270 280 290 300 QNEPLNSQAG YPTMYMFSYE QGDLIQNYVA PALKAAGLST KIWAYDHNTD QPDFPEQVMG 310 320 330 340 350 360 IAADDVSAVA WHCYATNLDW TVLTNFHNSY PNTDQYMTEC WTPSTGAWNQ AASFTMGPLQ 370 380 390 400 410 420 NWARGVAAWT LGTTAQDGPH LSSGGCGTCT GLVTINNGQY TFQTAYYMMA QFSKFMPVGA 430 440 450 460 470 480 TVLSGTGSYT YSGSGGVQSV ASLNPDGTRT VVIENTFGND IYIHLSTSSG QEWSGNVPTN 490 SVTTWVLPAV

References

[1]

Montero M. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ATCC 48131 / CBS 354.33 / CECT 2413 / VTT D-80150.

[2]

"BGN16.3, a novel acidic beta-1,6-glucanase from mycoparasitic fungus Trichoderma harzianum CECT 2413." Montero M., Sanz L., Rey M., Monte E., Llobell A. FEBS J. 272:3441-3448(2005) [PubMed: 15982190] [Abstract] Cited for: PROTEIN SEQUENCE OF 29-42 AND 168-180, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, INDUCTION. Strain: ATCC 48131 / CBS 354.33 / CECT 2413 / VTT D-80150.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ243823 mRNA. Translation: CAC80492.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH30. Glycoside Hydrolase Family 30.
Enzyme and pathway databases
BRENDA	3.2.1.75. 3745.
Family and domain databases
InterPro	IPR001139. Glyco_hydro_30. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR11069. Glyco_hydro_30. 1 hit.
Pfam	PF02055. Glyco_hydro_30. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN16_TRIHA

Accession

Primary (citable) accession number: Q8J0I9
Secondary accession number(s): P84511

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)