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Q8J093 (MPI_OGAPD) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mannose-6-phosphate isomerase

EC=5.3.1.8
Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name=PMI
Gene names
Name:PMI40
ORF Names:HPODL_0759
OrganismOgataea parapolymorpha (strain DL-1 / ATCC 26012 / NRRL Y-7560) (Yeast) (Hansenula polymorpha) [Complete proteome]
Taxonomic identifier871575 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetales incertae sedisOgataea

Protein attributes

Sequence length426 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions By similarity.

Catalytic activity

D-mannose 6-phosphate = D-fructose 6-phosphate.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Pathway

Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the mannose-6-phosphate isomerase type 1 family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMetal-binding
Zinc
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processGDP-mannose biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmannose-6-phosphate isomerase activity

Inferred from electronic annotation. Source: UniProtKB-EC

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 426426Mannose-6-phosphate isomerase
PRO_0000194246

Sites

Active site2961 By similarity
Metal binding1121Zinc By similarity
Metal binding1141Zinc By similarity
Metal binding1391Zinc By similarity
Metal binding2771Zinc By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8J093 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 8247706E7BECEE35

FASTA42646,779
        10         20         30         40         50         60 
MSSAPKLFRV IGGAQNYDWG KLGSTSAVAR FAKLNDPENV SIEEEKPYAE LWMGTHPSVP 

        70         80         90        100        110        120 
TVSAQDRTPL RDLVRAAPEE MLGQDIIDKF GSKEGIPFLF KVLSIRKVLS IQAHPDKALA 

       130        140        150        160        170        180 
RQLHASDPKH YPDDNHKPEM AVAITDFEAF CGFKPLAEID ALLQKIPEFR ELVGDDVVAE 

       190        200        210        220        230        240 
FHSGIDSSDV AGKKKLLQKV FSRVMNSPES KFEPLAARLV ERTKSDPQLF GETLADLIQR 

       250        260        270        280        290        300 
LDAQFPNDIG LFCGCLLLNH CILKSGEAMF LEAKDPHAYI SGDIMECMAA SDNVIRAGFT 

       310        320        330        340        350        360 
PKFKDVEVLV DCLTYSFNPV EEQKLKPAPF PRGTGVAELK LYDPPIDEFS VLQTTFTSAG 

       370        380        390        400        410        420 
AEKFEGLDGP SLLIVTEGKG KIKLQGSDEA LDASTGNIFF VAPKAAIELV SESKDTFTSY 


RAFCEA 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing and functional analysis of the Hansenula polymorpha genomic fragment containing the YPT1 and PMI40 genes."
Kim M.W., Agaphonov M.O., Kim J.Y., Rhee S.K., Kang H.A.
Yeast 19:863-871(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 26012 / NRRL Y-7560 / DL-1.
[2]"Molecular cloning of the Hansenula polymorpha homolog of the Saccharomyces cerevisiae PMI40 gene."
Kim M.W., Agaphonov M.O., Rhee S.K., Kang H.A.
Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 26012 / NRRL Y-7560 / DL-1.
[3]"Genome sequence of the methylotrophic yeast Hansenula polymorpha DL1."
Ravin N.V., Mardanov A.V., Eldarov M.A., Kadnikov V.V., Beletsky A.V., Zvereva M.I., Smekalova E.M., Dontsova O.A., Skryabin K.G.
Submitted (JAN-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 26012 / NRRL Y-7560 / DL-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF454544 Genomic DNA. Translation: AAN52529.1.
AF346819 Genomic DNA. Translation: AAN64443.1.
AEOI01000003 Genomic DNA. Translation: EFW98129.1.

3D structure databases

ProteinModelPortalQ8J093.
SMRQ8J093. Positions 3-426.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

OrthoDBEOG7WDNCR.

Enzyme and pathway databases

UniPathwayUPA00126; UER00423.

Family and domain databases

Gene3D2.60.120.10. 3 hits.
InterProIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERPTHR10309. PTHR10309. 1 hit.
PfamPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSPR00714. MAN6PISMRASE.
SUPFAMSSF51182. SSF51182. 1 hit.
TIGRFAMsTIGR00218. manA. 1 hit.
PROSITEPS00965. PMI_I_1. 1 hit.
PS00966. PMI_I_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPI_OGAPD
AccessionPrimary (citable) accession number: Q8J093
Secondary accession number(s): E7R1T0
Entry history
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: March 1, 2003
Last modified: February 19, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways