Reviewed, UniProtKB/Swiss-Prot Q8J093 (MPI_PICAN)

Names and origin

Protein names

Recommended name:
    Mannose-6-phosphate isomerase
    EC=5.3.1.8
Alternative name(s):
    Phosphomannose isomerase
      Short name=PMI
    Phosphohexomutase

Gene names

Name:

PMI40

Organism

Pichia angusta (Yeast) (Hansenula polymorpha)

Taxonomic identifier

4905 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions By similarity.
Catalytic activity	D-mannose 6-phosphate = D-fructose 6-phosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mannose-6-phosphate isomerase type 1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	mannose-6-phosphate isomerase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Mannose-6-phosphate isomerase		PRO_0000194246
Sites
	Active site	296	1	By similarity
	Metal binding	112	1	Zinc By similarity
	Metal binding	114	1	Zinc By similarity
	Metal binding	139	1	Zinc By similarity
	Metal binding	277	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8J093-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 8247706E7BECEE35	FASTA	426	46,779
	10 20 30 40 50 60 MSSAPKLFRV IGGAQNYDWG KLGSTSAVAR FAKLNDPENV SIEEEKPYAE LWMGTHPSVP 70 80 90 100 110 120 TVSAQDRTPL RDLVRAAPEE MLGQDIIDKF GSKEGIPFLF KVLSIRKVLS IQAHPDKALA 130 140 150 160 170 180 RQLHASDPKH YPDDNHKPEM AVAITDFEAF CGFKPLAEID ALLQKIPEFR ELVGDDVVAE 190 200 210 220 230 240 FHSGIDSSDV AGKKKLLQKV FSRVMNSPES KFEPLAARLV ERTKSDPQLF GETLADLIQR 250 260 270 280 290 300 LDAQFPNDIG LFCGCLLLNH CILKSGEAMF LEAKDPHAYI SGDIMECMAA SDNVIRAGFT 310 320 330 340 350 360 PKFKDVEVLV DCLTYSFNPV EEQKLKPAPF PRGTGVAELK LYDPPIDEFS VLQTTFTSAG 370 380 390 400 410 420 AEKFEGLDGP SLLIVTEGKG KIKLQGSDEA LDASTGNIFF VAPKAAIELV SESKDTFTSY RAFCEA

References

[1]	"Sequencing and functional analysis of the Hansenula polymorpha genomic fragment containing the YPT1 and PMI40 genes." Kim M.W., Agaphonov M.O., Kim J.Y., Rhee S.K., Kang H.A. Yeast 19:863-871(2002) [PubMed: 12112240] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 26012 / DL-1 / NRRL Y-7560.
[2]	"Molecular cloning of the Hansenula polymorpha homolog of the Saccharomyces cerevisiae PMI40 gene." Kim M.W., Agaphonov M.O., Rhee S.K., Kang H.A. Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 26012 / DL-1 / NRRL Y-7560.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF454544 Genomic DNA. Translation: AAN52529.1. AF346819 Genomic DNA. Translation: AAN64443.1.
3D structure databases
SMR	Q8J093. Positions 3-426.
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.3.1.8. 276392.
Family and domain databases
InterPro	IPR011051. Cupin_RmlC_type. IPR001250. Man6P_Isoase-1. IPR016305. Mannose-6-P_Isomerase. IPR018050. Pmannose_isomerase-type1_CS. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 2 hits.
Pfam	PF01238. PMI_typeI. 1 hit. [Graphical view]
PIRSF	PIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTS	PR00714. MAN6PISMRASE.
TIGRFAMs	TIGR00218. manA. 1 hit.
PROSITE	PS00965. PMI_I_1. 1 hit. PS00966. PMI_I_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPI_PICAN

Accession

Primary (citable) accession number: Q8J093

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)