Q8IZW8 (TENS4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tensin-4

Alternative name(s):
C-terminal tensin-like protein

Gene names

Name:	TNS4
Synonyms:	CTEN
ORF Names:	PP14434

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	715 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in cell migration, cartilage development and in linking signal transduction pathways to the cytoskeleton By similarity. May promote apoptosis, via its cleavage by caspase-3.
Subunit structure	Binds to actin filaments and interacts with phosphotyrosine-containing proteins By similarity.
Subcellular location	Cell junction › focal adhesion. Cytoplasm › cytoskeleton Ref.1.
Tissue specificity	Prostate and placenta. Down regulated in prostate cancer. Ref.1
Post-translational modification	Proteolytically cleaved by caspase-3 during apoptosis. Ref.6
Sequence similarities	Contains 1 phosphatase tensin-type domain. Contains 1 SH2 domain.
Sequence caution	The sequence AAQ15257.1 differs from that shown. Reason: Frameshift at positions 85, 296, 472 and 602.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cell junction Cytoplasm Cytoskeleton
Coding sequence diversity	Polymorphism
Domain	SH2 domain Signal
Ligand	Actin-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW protein localization Inferred from direct assay PubMed 17190795. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell focal adhesion Inferred from direct assay PubMed 17190795. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 715	697	Tensin-4		PRO_0000248213
Regions
	Domain	449 – 556	108	SH2
	Domain	548 – 714	167	Phosphatase tensin-type
	Compositional bias	168 – 324	157	Ser-rich
Sites
	Site	570 – 571	2	Cleavage; by caspase-3
Amino acid modifications
	Modified residue	82	1	Phosphoserine Ref.7
	Modified residue	248	1	Phosphoserine Ref.7
Natural variations
	Natural variant	179	1	L → P. Ref.1 Corresponds to variant rs3764424 [ dbSNP | Ensembl ].		VAR_027264
	Natural variant	327	1	T → K. Corresponds to variant rs33923045 [ dbSNP | Ensembl ].		VAR_055292
	Natural variant	498	1	S → N. Ref.1 Ref.3 Corresponds to variant rs2290207 [ dbSNP | Ensembl ].		VAR_027265
	Natural variant	642	1	R → C in a colorectal cancer sample; somatic mutation. Ref.9		VAR_036515
Experimental info
	Mutagenesis	506	1	D → A: No effect on cleavage by caspase-3. Ref.6
	Mutagenesis	570	1	D → A: Abolishes cleavage by caspase-3. Ref.6
	Sequence conflict	163	1	G → W in AAN32666. Ref.1
	Sequence conflict	360	1	S → I in AAN32666. Ref.1
	Sequence conflict	360	1	S → I in BAB55413. Ref.3
	Sequence conflict	623	1	K → E in AAN32666. Ref.1
	Sequence conflict	623	1	K → E in BAB55413. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8IZW8 [UniParc]. Last modified May 5, 2009. Version 3. Checksum: E6AD7CB6B6DDA5D2	FASTA	715	76,764
	10 20 30 40 50 60 MSQVMSSPLL AGGHAVSLAP CDEPRRTLHP APSPSLPPQC SYYTTEGWGA QALMAPVPCM 70 80 90 100 110 120 GPPGRLQQAP QVEAKATCFL PSPGEKALGT PEDLDSYIDF SLESLNQMIL ELDPTFQLLP 130 140 150 160 170 180 PGTGGSQAEL AQSTMSMRKK EESEALDIKY IEVTSARSRC HDGPQHCSSP SVTPPFGSLR 190 200 210 220 230 240 SGGLLLSRDV PRETRSSSES LIFSGNQGRG HQRPLPPSEG LSPRPPNSPS ISIPCMGSKA 250 260 270 280 290 300 SSPHGLGSPL VASPRLEKRL GGLAPQRGSR ISVLSASPVS DVSYMFGSSQ SLLHSSNSSH 310 320 330 340 350 360 QSSSRSLESP ANSSSSLHSL GSVSLCTRPS DFQAPRNPTL TMGQPRTPHS PPLAKEHASS 370 380 390 400 410 420 CPPSITNSMV DIPIVLINGC PEPGSSPPQR TPGHQNSVQP GAASPSNPCP ATRSNSQTLS 430 440 450 460 470 480 DAPFTTCPEG PARDMQPTMK FVMDTSKYWF KPNITREQAI ELLRKEEPGA FVIRDSSSYR 490 500 510 520 530 540 GSFGLALKVQ EVPASAQSRP GEDSNDLIRH FLIESSAKGV HLKGADEEPY FGSLSAFVCQ 550 560 570 580 590 600 HSIMALALPC KLTIPQRELG GADGASDSTD SPASCQKKSA GCHTLYLSSV SVETLTGALA 610 620 630 640 650 660 VQKAISTTFE RDILPTPTVV HFKVTEQGIT LTDVQRKVFF RRHYPLTTLR FCGMDPEQRK 670 680 690 700 710 WQKYCKPSWI FGFVAKSQTE PQENVCHLFA EYDMVQPASQ VIGLVTALLQ DAERM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cten, a COOH-terminal tensin-like protein with prostate restricted expression, is down-regulated in prostate cancer." Lo S.H., Lo T.B. Cancer Res. 62:4217-4221(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, VARIANTS PRO-179 AND ASN-498.
[2]	"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASN-498. Tissue: Placenta.
[4]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[6]	"Cleavage of cten by caspase-3 during apoptosis." Lo S.-S., Lo S.H., Lo S.H. Oncogene 24:4311-4314(2005) [PubMed] [Europe PMC] [Abstract] Cited for: CLEAVAGE BY CASPASE-3, MUTAGENESIS OF ASP-506 AND ASP-570.
[7]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-82 AND SER-248, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] CYS-642.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF417488 mRNA. Translation: AAN32666.1. AF370421 mRNA. Translation: AAQ15257.1. Frameshift. AK027856 mRNA. Translation: BAB55413.1. AC018629 Genomic DNA. No translation available. BC018706 mRNA. Translation: AAH18706.1.
IPI	IPI00170752.
RefSeq	NP_116254.4. NM_032865.5.
UniGene	Hs.438292.
3D structure databases
HSSP	HSSP built from PDB template 1WVH based on UniProtKB Q04205.
ProteinModelPortal	Q8IZW8.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1493010.
STRING	9606.ENSP00000254051.
PTM databases
PhosphoSite	Q8IZW8.
Polymorphism databases
DMDM	229463025.
Proteomic databases
PaxDb	Q8IZW8.
PRIDE	Q8IZW8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000254051; ENSP00000254051; ENSG00000131746.
GeneID	84951.
KEGG	hsa:84951.
UCSC	uc010cxb.3. human.
Organism-specific databases
CTD	84951.
GeneCards	GC17M038632.
H-InvDB	HIX0013799.
HGNC	HGNC:24352. TNS4.
HPA	HPA021913.
MIM	608385. gene.
neXtProt	NX_Q8IZW8.
PharmGKB	PA142670717.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG254290.
HOGENOM	HOG000230981.
HOVERGEN	HBG080039.
InParanoid	Q8IZW8.
OMA	LNQMILE.
OrthoDB	EOG4VT5WP.
PhylomeDB	Q8IZW8.
Gene expression databases
Bgee	Q8IZW8.
CleanEx	HS_TNS4.
Genevestigator	Q8IZW8.
GermOnline	ENSG00000131746. Homo sapiens.
Family and domain databases
Gene3D	2.30.29.30. 1 hit. 3.30.505.10. 1 hit.
InterPro	IPR011993. PH_like_dom. IPR013625. PTB. IPR000980. SH2. [Graphical view]
Pfam	PF08416. PTB. 1 hit. PF00017. SH2. 1 hit. [Graphical view]
SMART	SM00252. SH2. 1 hit. [Graphical view]
PROSITE	PS51181. PPASE_TENSIN. False negative. PS50001. SH2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TNS4. human.
GenomeRNAi	84951.
NextBio	75438.
PMAP-CutDB	Q8IZW8.
SOURCE	Search...

Entry information

Entry name

TENS4_HUMAN

Accession

Primary (citable) accession number: Q8IZW8
Secondary accession number(s): A6NMJ7 , Q71RB7, Q8WV64, Q96JV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	May 5, 2009
Last modified:	May 1, 2013
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.