Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8IZQ1

- WDFY3_HUMAN

UniProt

Q8IZQ1 - WDFY3_HUMAN

Protein

WD repeat and FYVE domain-containing protein 3

Gene

WDFY3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 2 (27 Jun 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Required for selective autophagy (aggrephagy) but not for autophagic degradation of bulk cytospol in response to starvation. Involved in the formation and degradation of cytoplasmic polyubiquitin-containing bodies (p62 bodies, ALIS/aggresome-like induced structures). May play a role as adaptor or scaffolding protein by promoting the association of the E3-like ligase ATG12-ATG5-ATG16L and LC3 to ubiquitinated target substrate. The association with GABARAP is required for its recruitment to LC3B-positive p62 bodies suggesting a role in targeting certain p62 structures for clearance. Involved in midbody ring degradation.3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri3454 – 351461FYVE-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. 1-phosphatidylinositol binding Source: MGI
    2. beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Source: Ensembl
    3. metal ion binding Source: UniProtKB-KW
    4. protein binding Source: UniProtKB

    GO - Biological processi

    1. aggrephagy Source: UniProtKB
    2. positive regulation of macroautophagy Source: UniProtKB

    Keywords - Biological processi

    Autophagy

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    SignaLinkiQ8IZQ1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    WD repeat and FYVE domain-containing protein 3
    Alternative name(s):
    Autophagy-linked FYVE protein
    Short name:
    Alfy
    Gene namesi
    Name:WDFY3
    Synonyms:KIAA0993
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:20751. WDFY3.

    Subcellular locationi

    Nucleus membrane. Cytoplasm. NucleusPML body. Membrane; Peripheral membrane protein; Cytoplasmic side
    Note: Colocalizes with autophagic structures in starved cells. Shuttles between nucleus and cytoplasm; translocation from the nucleus to cytoplasmic polyubiquitin-containing bodies upon cellular stress is dependent on SQSTM1.

    GO - Cellular componenti

    1. autophagic vacuole Source: UniProtKB
    2. cytoplasm Source: UniProtKB
    3. extrinsic component of membrane Source: MGI
    4. inclusion body Source: UniProtKB
    5. nuclear envelope Source: UniProtKB
    6. PML body Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Membrane, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi3343 – 33431K → A: Decreases interaction with GABARAP and does not increase interaction with MAP1LC3B; when associated with A-3344 and A-3351. 1 Publication
    Mutagenesisi3344 – 33441D → A: Decreases interaction with GABARAP and does not increase interaction with MAP1LC3B; when associated with A-3343 and A-3351. 1 Publication
    Mutagenesisi3346 – 33461F → A: Abolishes interaction with GABARAP and MAP1LC3C. 1 Publication
    Mutagenesisi3347 – 33471I → A: Decreases interaction with GABARAP and MAP1LC3C. 1 Publication
    Mutagenesisi3348 – 33481F → A: Decreases interaction with GABARAP and MAP1LC3C. 1 Publication
    Mutagenesisi3349 – 33491V → A: Decreases interaction with GABARAP and MAP1LC3C. 1 Publication
    Mutagenesisi3351 – 33511Y → A: Decreases interaction with GABARAP and does not increase interaction with MAP1LC3B; when associated with A-3343 and A-3344. 1 Publication

    Organism-specific databases

    PharmGKBiPA134903706.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 35263526WD repeat and FYVE domain-containing protein 3PRO_0000242693Add
    BLAST

    Proteomic databases

    MaxQBiQ8IZQ1.
    PaxDbiQ8IZQ1.
    PRIDEiQ8IZQ1.

    PTM databases

    PhosphoSiteiQ8IZQ1.

    Expressioni

    Tissue specificityi

    Ubiquitous. Expressed in osteoclast and their mononuclear precursors (at protein level).2 Publications

    Gene expression databases

    ArrayExpressiQ8IZQ1.
    BgeeiQ8IZQ1.
    CleanExiHS_WDFY3.
    GenevestigatoriQ8IZQ1.

    Organism-specific databases

    HPAiHPA042734.
    HPA048572.

    Interactioni

    Subunit structurei

    Interacts with ATG5 and SQSTM1. Associates with the ATG12-ATG5-ATG16L complex. Interacts with GABARAP, GABARAPL1 and GABARAPL2, and weakly with MAP1LC3C.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ATG5Q9H1Y07EBI-1569256,EBI-1047414
    HTTP4285810EBI-1569256,EBI-466029

    Protein-protein interaction databases

    BioGridi116647. 12 interactions.
    IntActiQ8IZQ1. 14 interactions.
    MINTiMINT-4829131.

    Structurei

    Secondary structure

    1
    3526
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3346 – 33483

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3WIMX-ray2.60B3341-3354[»]
    ProteinModelPortaliQ8IZQ1.
    SMRiQ8IZQ1. Positions 2611-2976, 3047-3245, 3409-3441, 3448-3513.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2683 – 2976294BEACHPROSITE-ProRule annotationAdd
    BLAST
    Repeati3077 – 311539WD 1Add
    BLAST
    Repeati3125 – 316440WD 2Add
    BLAST
    Repeati3167 – 320640WD 3Add
    BLAST
    Repeati3210 – 325445WD 4Add
    BLAST
    Repeati3408 – 344740WD 5Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2285 – 2981697Sufficient for translocalization to p62 bodies/ALISAdd
    BLAST
    Regioni2586 – 3526941Interaction with SQSTM1Add
    BLAST
    Regioni2981 – 3526546Interaction with ATG5Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi3344 – 33496LIR

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi472 – 4754Poly-Ser
    Compositional biasi2516 – 25194Poly-Glu

    Domaini

    The LIR (LC3-interacting region) motif mediates the interaction with ATG8 family proteins.

    Sequence similaritiesi

    Contains 1 BEACH domain.PROSITE-ProRule annotation
    Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation
    Contains 5 WD repeats.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri3454 – 351461FYVE-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, WD repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG236271.
    HOVERGENiHBG094156.
    InParanoidiQ8IZQ1.
    OMAiQEEKLYM.
    OrthoDBiEOG70CR5S.
    PhylomeDBiQ8IZQ1.
    TreeFamiTF313658.

    Family and domain databases

    Gene3Di1.10.1540.10. 1 hit.
    1.25.10.10. 2 hits.
    2.130.10.10. 2 hits.
    2.30.29.40. 1 hit.
    3.30.40.10. 1 hit.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR000409. BEACH_dom.
    IPR008985. ConA-like_lec_gl_sf.
    IPR023362. PH-BEACH_dom.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    IPR000306. Znf_FYVE.
    IPR017455. Znf_FYVE-rel.
    IPR011011. Znf_FYVE_PHD.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PfamiPF02138. Beach. 1 hit.
    PF01363. FYVE. 1 hit.
    PF14844. PH_BEACH. 1 hit.
    PF00400. WD40. 2 hits.
    [Graphical view]
    SMARTiSM01026. Beach. 1 hit.
    SM00064. FYVE. 1 hit.
    SM00320. WD40. 5 hits.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 3 hits.
    SSF49899. SSF49899. 1 hit.
    SSF50978. SSF50978. 2 hits.
    SSF57903. SSF57903. 1 hit.
    SSF81837. SSF81837. 1 hit.
    PROSITEiPS50197. BEACH. 1 hit.
    PS00678. WD_REPEATS_1. 1 hit.
    PS50082. WD_REPEATS_2. 1 hit.
    PS50294. WD_REPEATS_REGION. 1 hit.
    PS50178. ZF_FYVE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8IZQ1-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNMVKRIMGR PRQEECSPQD NALGLMHLRR LFTELCHPPR HMTQKEQEEK     50
    LYMMLPVFNR VFGNAPPNTM TEKFSDLLQF TTQVSRLMVT EIRRRASNKS 100
    TEAASRAIVQ FLEINQSEEA SRGWMLLTTI NLLASSGQKT VDCMTTMSVP 150
    STLVKCLYLF FDLPHVPEAV GGAQNELPLA ERRGLLQKVF VQILVKLCSF 200
    VSPAEELAQK DDLQLLFSAI TSWCPPYNLP WRKSAGEVLM TISRHGLSVN 250
    VVKYIHEKEC LSTCVQNMQQ SDDLSPLEIV EMFAGLSCFL KDSSDVSQTL 300
    LDDFRIWQGY NFLCDLLLRL EQAKEAESKD ALKDLVNLIT SLTTYGVSEL 350
    KPAGITTGAP FLLPGFAVPQ PAGKGHSVRN VQAFAVLQNA FLKAKTSFLA 400
    QIILDAITNI YMADNANYFI LESQHTLSQF AEKISKLPEV QNKYFEMLEF 450
    VVFSLNYIPC KELISVSILL KSSSSYHCSI IAMKTLLKFT RHDYIFKDVF 500
    REVGLLEVMV NLLHKYAALL KDPTQALNEQ GDSRNNSSVE DQKHLALLVM 550
    ETLTVLLQGS NTNAGIFREF GGARCAHNIV KYPQCRQHAL MTIQQLVLSP 600
    NGDDDMGTLL GLMHSAPPTE LQLKTDILRA LLSVLRESHR SRTVFRKVGG 650
    FVYITSLLVA MERSLSCPPK NGWEKVNQNQ VFELLHTVFC TLTAAMRYEP 700
    ANSHFFKTEI QYEKLADAVR FLGCFSDLRK ISAMNVFPSN TQPFQRLLEE 750
    DVISIESVSP TLRHCSKLFI YLYKVATDSF DSRAEQIPPC LTSESSLPSP 800
    WGTPALSRKR HAYHSVSTPP VYPPKNVADL KLHVTTSSLQ SSDAVIIHPG 850
    AMLAMLDLLA SVGSVTQPEH ALDLQLAVAN ILQSLVHTER NQQVMCEAGL 900
    HARLLQRCSA ALADEDHSLH PPLQRMFERL ASQALEPMVL REFLRLASPL 950
    NCGAWDKKLL KQYRVHKPSS LSYEPEMRSS MITSLEGLGT DNVFSLHEDN 1000
    HYRISKSLVK SAEGSTVPLT RVKCLVSMTT PHDIRLHGSS VTPAFVEFDT 1050
    SLEGFGCLFL PSLAPHNAPT NNTVTTGLID GAVVSGIGSG ERFFPPPSGL 1100
    SYSSWFCIEH FSSPPNNHPV RLLTVVRRAN SSEQHYVCLA IVLSAKDRSL 1150
    IVSTKEELLQ NYVDDFSEES SFYEILPCCA RFRCGELIIE GQWHHLVLVM 1200
    SKGMLKNSTA ALYIDGQLVN TVKLHYVHST PGGSGSANPP VVSTVYAYIG 1250
    TPPAQRQIAS LVWRLGPTHF LEEVLPSSNV TTIYELGPNY VGSFQAVCMP 1300
    CKDAKSEGVV PSPVSLVPEE KVSFGLYALS VSSLTVARIR KVYNKLDSKA 1350
    IAKQLGISSH ENATPVKLIH NSAGHLNGSA RTIGAALIGY LGVRTFVPKP 1400
    VATTLQYVGG AAAILGLVAM ASDVEGLYAA VKALVCVVKS NPLASKEMER 1450
    IKGYQLLAML LKKKRSLLNS HILHLTFSLV GTVDSGHETS IIPNSTAFQD 1500
    LLCDFEVWLH APYELHLSLF EHFIELLTES SEASKNAKLM REFQLIPKLL 1550
    LTLRDMSLSQ PTIAAISNVL SFLLQGFPSS NDLLRFGQFI SSTLPTFAVC 1600
    EKFVVMEINN EEKLDTGTEE EFGGLVSANL ILLRNRLLDI LLKLIYTSKE 1650
    KTSINLQACE ELVKTLGFDW IMMFMEEHLH STTVTAAMRI LVVLLSNQSI 1700
    LIKFKEGLSG GGWLEQTDSV LTNKIGTVLG FNVGRSAGGR STVREINRDA 1750
    CHFPGFPVLQ SFLPKHTNVP ALYFLLMALF LQQPVSELPE NLQVSVPVIS 1800
    CRSKQGCQFD LDSIWTFIFG VPASSGTVVS SIHNVCTEAV FLLLGMLRSM 1850
    LTSPWQSEEE GSWLREYPVT LMQFFRYLYH NVPDLASMWM SPDFLCALAA 1900
    TVFPFNIRPY SEMVTDLDDE VGSPAEEFKA FAADTGMNRS QSEYCNVGTK 1950
    TYLTNHPAKK FVFDFMRVLI IDNLCLTPAS KQTPLIDLLL EASPERSTRT 2000
    QQKEFQTYIL DSVMDHLLAA DVLLGEDASL PITSGGSYQV LVNNVFYFTQ 2050
    RVVDKLWQGM FNKESKLLID FIIQLIAQSK RRSQGLSLDA VYHCLNRTIL 2100
    YQFSRAHKTV PQQVALLDSL RVLTVNRNLI LGPGNHDQEF ISCLAHCLIN 2150
    LHVGSNVDGF GLEAEARMTT WHIMIPSDIE PDGSYSQDIS EGRQLLIKAV 2200
    NRVWTELIHS KKQVLEELFK VTLPVNERGH VDIATARPLI EEAALKCWQN 2250
    HLAHEKKCIS RGEALAPTTQ SKLSRVSSGF GLSKLTGSRR NRKESGLNKH 2300
    SLSTQEISQW MFTHIAVVRD LVDTQYKEYQ ERQQNALKYV TEEWCQIECE 2350
    LLRERGLWGP PIGSHLDKWM LEMTEGPCRM RKKMVRNDMF YNHYPYVPET 2400
    EQETNVASEI PSKQPETPDD IPQKKPARYR RAVSYDSKEY YMRLASGNPA 2450
    IVQDAIVESS EGEAAQQEPE HGEDTIAKVK GLVKPPLKRS RSAPDGGDEE 2500
    NQEQLQDQIA EGSSIEEEEK TDNATLLRLL EEGEKIQHMY RCARVQGLDT 2550
    SEGLLLFGKE HFYVIDGFTM TATREIRDIE TLPPNMHEPI IPRGARQGPS 2600
    QLKRTCSIFA YEDIKEVHKR RYLLQPIAVE VFSGDGRNYL LAFQKGIRNK 2650
    VYQRFLAVVP SLTDSSESVS GQRPNTSVEQ GSGLLSTLVG EKSVTQRWER 2700
    GEISNFQYLM HLNTLAGRSY NDLMQYPVFP WILADYDSEE VDLTNPKTFR 2750
    NLAKPMGAQT DERLAQYKKR YKDWEDPNGE TPAYHYGTHY SSAMIVASYL 2800
    VRMEPFTQIF LRLQGGHFDL ADRMFHSVRE AWYSASKHNM ADVKELIPEF 2850
    FYLPEFLFNS NNFDLGCKQN GTKLGDVILP PWAKGDPREF IRVHREALEC 2900
    DYVSAHLHEW IDLIFGYKQQ GPAAVEAVNV FHHLFYEGQV DIYNINDPLK 2950
    ETATIGFINN FGQIPKQLFK KPHPPKRVRS RLNGDNAGIS VLPGSTSDKI 3000
    FFHHLDNLRP SLTPVKELKE PVGQIVCTDK GILAVEQNKV LIPPTWNKTF 3050
    AWGYADLSCR LGTYESDKAM TVYECLSEWG QILCAICPNP KLVITGGTST 3100
    VVCVWEMGTS KEKAKTVTLK QALLGHTDTV TCATASLAYH IIVSGSRDRT 3150
    CIIWDLNKLS FLTQLRGHRA PVSALCINEL TGDIVSCAGT YIHVWSINGN 3200
    PIVSVNTFTG RSQQIICCCM SEMNEWDTQN VIVTGHSDGV VRFWRMEFLQ 3250
    VPETPAPEPA EVLEMQEDCP EAQIGQEAQD EDSSDSEADE QSISQDPKDT 3300
    PSQPSSTSHR PRAASCRATA AWCTDSGSDD SRRWSDQLSL DEKDGFIFVN 3350
    YSEGQTRAHL QGPLSHPHPN PIEVRNYSRL KPGYRWERQL VFRSKLTMHT 3400
    AFDRKDNAHP AEVTALGISK DHSRILVGDS RGRVFSWSVS DQPGRSAADH 3450
    WVKDEGGDSC SGCSVRFSLT ERRHHCRNCG QLFCQKCSRF QSEIKRLKIS 3500
    SPVRVCQNCY YNLQHERGSE DGPRNC 3526
    Length:3,526
    Mass (Da):395,258
    Last modified:June 27, 2006 - v2
    Checksum:iF4D518E8C9C12E23
    GO
    Isoform 2 (identifier: Q8IZQ1-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         2408-2424: Missing.

    Show »
    Length:3,509
    Mass (Da):393,367
    Checksum:i5DF8D0918CD685F6
    GO

    Sequence cautioni

    The sequence BAB71020.1 differs from that shown. Reason: Aberrant splicing.
    The sequence AAH13377.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAH15214.2 differs from that shown. Reason: Erroneous initiation.
    The sequence BAC04455.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2944 – 29441N → T in AAN15137. (PubMed:15292400)Curated
    Sequence conflicti3396 – 33961L → P in BAB71020. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti3032 – 30321I → V.
    Corresponds to variant rs17368018 [ dbSNP | Ensembl ].
    VAR_026864

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei2408 – 242417Missing in isoform 2. 1 PublicationVSP_019475Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF538685 mRNA. Translation: AAN15137.1.
    AC095046 Genomic DNA. Translation: AAY40903.1.
    AC104082 Genomic DNA. No translation available.
    AB023210 mRNA. Translation: BAA76837.2.
    AK055806 mRNA. Translation: BAB71020.1. Sequence problems.
    AK094910 mRNA. Translation: BAC04455.1. Different initiation.
    BC013377 mRNA. Translation: AAH13377.1. Different initiation.
    BC015214 mRNA. Translation: AAH15214.2. Different initiation.
    BC065502 mRNA. Translation: AAH65502.1.
    BC119633 mRNA. Translation: AAI19634.1.
    CCDSiCCDS3609.1. [Q8IZQ1-1]
    RefSeqiNP_055806.2. NM_014991.4. [Q8IZQ1-1]
    UniGeneiHs.480116.
    Hs.726339.

    Genome annotation databases

    EnsembliENST00000295888; ENSP00000295888; ENSG00000163625. [Q8IZQ1-1]
    GeneIDi23001.
    KEGGihsa:23001.
    UCSCiuc003hpd.3. human. [Q8IZQ1-1]

    Polymorphism databases

    DMDMi109896161.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF538685 mRNA. Translation: AAN15137.1 .
    AC095046 Genomic DNA. Translation: AAY40903.1 .
    AC104082 Genomic DNA. No translation available.
    AB023210 mRNA. Translation: BAA76837.2 .
    AK055806 mRNA. Translation: BAB71020.1 . Sequence problems.
    AK094910 mRNA. Translation: BAC04455.1 . Different initiation.
    BC013377 mRNA. Translation: AAH13377.1 . Different initiation.
    BC015214 mRNA. Translation: AAH15214.2 . Different initiation.
    BC065502 mRNA. Translation: AAH65502.1 .
    BC119633 mRNA. Translation: AAI19634.1 .
    CCDSi CCDS3609.1. [Q8IZQ1-1 ]
    RefSeqi NP_055806.2. NM_014991.4. [Q8IZQ1-1 ]
    UniGenei Hs.480116.
    Hs.726339.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3WIM X-ray 2.60 B 3341-3354 [» ]
    ProteinModelPortali Q8IZQ1.
    SMRi Q8IZQ1. Positions 2611-2976, 3047-3245, 3409-3441, 3448-3513.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 116647. 12 interactions.
    IntActi Q8IZQ1. 14 interactions.
    MINTi MINT-4829131.

    PTM databases

    PhosphoSitei Q8IZQ1.

    Polymorphism databases

    DMDMi 109896161.

    Proteomic databases

    MaxQBi Q8IZQ1.
    PaxDbi Q8IZQ1.
    PRIDEi Q8IZQ1.

    Protocols and materials databases

    DNASUi 23001.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000295888 ; ENSP00000295888 ; ENSG00000163625 . [Q8IZQ1-1 ]
    GeneIDi 23001.
    KEGGi hsa:23001.
    UCSCi uc003hpd.3. human. [Q8IZQ1-1 ]

    Organism-specific databases

    CTDi 23001.
    GeneCardsi GC04M085590.
    H-InvDB HIX0004348.
    HGNCi HGNC:20751. WDFY3.
    HPAi HPA042734.
    HPA048572.
    neXtProti NX_Q8IZQ1.
    PharmGKBi PA134903706.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG236271.
    HOVERGENi HBG094156.
    InParanoidi Q8IZQ1.
    OMAi QEEKLYM.
    OrthoDBi EOG70CR5S.
    PhylomeDBi Q8IZQ1.
    TreeFami TF313658.

    Enzyme and pathway databases

    SignaLinki Q8IZQ1.

    Miscellaneous databases

    ChiTaRSi WDFY3. human.
    GeneWikii WDFY3.
    GenomeRNAii 23001.
    NextBioi 43900.
    PROi Q8IZQ1.

    Gene expression databases

    ArrayExpressi Q8IZQ1.
    Bgeei Q8IZQ1.
    CleanExi HS_WDFY3.
    Genevestigatori Q8IZQ1.

    Family and domain databases

    Gene3Di 1.10.1540.10. 1 hit.
    1.25.10.10. 2 hits.
    2.130.10.10. 2 hits.
    2.30.29.40. 1 hit.
    3.30.40.10. 1 hit.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR000409. BEACH_dom.
    IPR008985. ConA-like_lec_gl_sf.
    IPR023362. PH-BEACH_dom.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    IPR000306. Znf_FYVE.
    IPR017455. Znf_FYVE-rel.
    IPR011011. Znf_FYVE_PHD.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    Pfami PF02138. Beach. 1 hit.
    PF01363. FYVE. 1 hit.
    PF14844. PH_BEACH. 1 hit.
    PF00400. WD40. 2 hits.
    [Graphical view ]
    SMARTi SM01026. Beach. 1 hit.
    SM00064. FYVE. 1 hit.
    SM00320. WD40. 5 hits.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 3 hits.
    SSF49899. SSF49899. 1 hit.
    SSF50978. SSF50978. 2 hits.
    SSF57903. SSF57903. 1 hit.
    SSF81837. SSF81837. 1 hit.
    PROSITEi PS50197. BEACH. 1 hit.
    PS00678. WD_REPEATS_1. 1 hit.
    PS50082. WD_REPEATS_2. 1 hit.
    PS50294. WD_REPEATS_REGION. 1 hit.
    PS50178. ZF_FYVE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Alfy, a novel FYVE-domain-containing protein associated with protein granules and autophagic membranes."
      Simonsen A., Birkeland H.C.G., Gillooly D.J., Mizushima N., Kuma A., Yoshimori T., Slagsvold T., Brech A., Stenmark H.
      J. Cell Sci. 117:4239-4251(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Brain.
    2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
      Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
      DNA Res. 6:63-70(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1971-3526 (ISOFORM 1).
      Tissue: Brain.
    4. "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
      Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
      DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION.
    5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2381-3036 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2650-3526 (ISOFORM 1).
      Tissue: Corpus callosum and Kidney.
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2749-3526 (ISOFORM 1).
      Tissue: Colon, Duodenum and Ovary.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. "p62/SQSTM1 and ALFY interact to facilitate the formation of p62 bodies/ALIS and their degradation by autophagy."
      Clausen T.H., Lamark T., Isakson P., Finley K., Larsen K.B., Brech A., Overvatn A., Stenmark H., Bjorkoy G., Simonsen A., Johansen T.
      Autophagy 6:330-344(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SQSTM1, SUBCELLULAR LOCATION.
    9. "Functional interaction between sequestosome-1/p62 and autophagy-linked FYVE-containing protein WDFY3 in human osteoclasts."
      Hocking L.J., Mellis D.J., McCabe P.S., Helfrich M.H., Rogers M.J.
      Biochem. Biophys. Res. Commun. 402:543-548(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SQSTM1, TISSUE SPECIFICITY.
    10. "The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy."
      Filimonenko M., Isakson P., Finley K.D., Anderson M., Jeong H., Melia T.J., Bartlett B.J., Myers K.M., Birkeland H.C., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A.
      Mol. Cell 38:265-279(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ATG5, ASSOCIATION WITH THE ATG12-ATG5-ATG16L COMPLEX, SUBCELLULAR LOCATION.
    11. "TRAF6 mediates ubiquitination of KIF23/MKLP1 and is required for midbody ring degradation by selective autophagy."
      Isakson P., Lystad A.H., Breen K., Koster G., Stenmark H., Simonsen A.
      Autophagy 9:1955-1964(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "Structural determinants in GABARAP required for the selective binding and recruitment of ALFY to LC3B-positive structures."
      Lystad A.H., Ichimura Y., Takagi K., Yang Y., Pankiv S., Kanegae Y., Kageyama S., Suzuki M., Saito I., Mizushima T., Komatsu M., Simonsen A.
      EMBO Rep. 15:557-565(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 3341-3354 IN COMPLEX WITH GABARAP, INTERACTION WITH MAP1LC3C; GABARAPL1 AND GABARAPL2, LIR MOTIF, MUTAGENESIS OF LYS-3343; ASP-3344; PHE-3346; ILE-3347; PHE-3348; VAL-3349 AND TYR-3351.

    Entry informationi

    Entry nameiWDFY3_HUMAN
    AccessioniPrimary (citable) accession number: Q8IZQ1
    Secondary accession number(s): Q4W5K5
    , Q6P0Q5, Q8N1T2, Q8NAV6, Q96BS7, Q96D33, Q96N85, Q9Y2J7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 27, 2006
    Last sequence update: June 27, 2006
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3