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Q8IZQ1

- WDFY3_HUMAN

UniProt

Q8IZQ1 - WDFY3_HUMAN

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Protein

WD repeat and FYVE domain-containing protein 3

Gene

WDFY3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for selective autophagy (aggrephagy) but not for autophagic degradation of bulk cytospol in response to starvation. Involved in the formation and degradation of cytoplasmic polyubiquitin-containing bodies (p62 bodies, ALIS/aggresome-like induced structures). May play a role as adaptor or scaffolding protein by promoting the association of the E3-like ligase ATG12-ATG5-ATG16L and LC3 to ubiquitinated target substrate. The association with GABARAP is required for its recruitment to LC3B-positive p62 bodies suggesting a role in targeting certain p62 structures for clearance. Involved in midbody ring degradation.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3454 – 351461FYVE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. 1-phosphatidylinositol binding Source: MGI
  2. beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Source: Ensembl
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. aggrephagy Source: UniProtKB
  2. positive regulation of macroautophagy Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Autophagy

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

SignaLinkiQ8IZQ1.

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat and FYVE domain-containing protein 3
Alternative name(s):
Autophagy-linked FYVE protein
Short name:
Alfy
Gene namesi
Name:WDFY3
Synonyms:KIAA0993
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 4

Organism-specific databases

HGNCiHGNC:20751. WDFY3.

Subcellular locationi

Nucleus membrane. Cytoplasm. NucleusPML body. Membrane; Peripheral membrane protein; Cytoplasmic side
Note: Colocalizes with autophagic structures in starved cells. Shuttles between nucleus and cytoplasm; translocation from the nucleus to cytoplasmic polyubiquitin-containing bodies upon cellular stress is dependent on SQSTM1.

GO - Cellular componenti

  1. autophagic vacuole Source: UniProtKB
  2. cytoplasm Source: UniProtKB
  3. extrinsic component of membrane Source: MGI
  4. inclusion body Source: UniProtKB
  5. nuclear envelope Source: UniProtKB
  6. PML body Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi3343 – 33431K → A: Decreases interaction with GABARAP and does not increase interaction with MAP1LC3B; when associated with A-3344 and A-3351. 1 Publication
Mutagenesisi3344 – 33441D → A: Decreases interaction with GABARAP and does not increase interaction with MAP1LC3B; when associated with A-3343 and A-3351. 1 Publication
Mutagenesisi3346 – 33461F → A: Abolishes interaction with GABARAP and MAP1LC3C. 1 Publication
Mutagenesisi3347 – 33471I → A: Decreases interaction with GABARAP and MAP1LC3C. 1 Publication
Mutagenesisi3348 – 33481F → A: Decreases interaction with GABARAP and MAP1LC3C. 1 Publication
Mutagenesisi3349 – 33491V → A: Decreases interaction with GABARAP and MAP1LC3C. 1 Publication
Mutagenesisi3351 – 33511Y → A: Decreases interaction with GABARAP and does not increase interaction with MAP1LC3B; when associated with A-3343 and A-3344. 1 Publication

Organism-specific databases

PharmGKBiPA134903706.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 35263526WD repeat and FYVE domain-containing protein 3PRO_0000242693Add
BLAST

Proteomic databases

MaxQBiQ8IZQ1.
PaxDbiQ8IZQ1.
PRIDEiQ8IZQ1.

PTM databases

PhosphoSiteiQ8IZQ1.

Expressioni

Tissue specificityi

Ubiquitous. Expressed in osteoclast and their mononuclear precursors (at protein level).2 Publications

Gene expression databases

BgeeiQ8IZQ1.
CleanExiHS_WDFY3.
ExpressionAtlasiQ8IZQ1. baseline and differential.
GenevestigatoriQ8IZQ1.

Organism-specific databases

HPAiHPA042734.
HPA048572.

Interactioni

Subunit structurei

Interacts with ATG5 and SQSTM1. Associates with the ATG12-ATG5-ATG16L complex. Interacts with GABARAP, GABARAPL1 and GABARAPL2, and weakly with MAP1LC3C.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
P140793EBI-1569256,EBI-9675698From a different organism.
ATG5Q9H1Y07EBI-1569256,EBI-1047414
HTTP4285810EBI-1569256,EBI-466029

Protein-protein interaction databases

BioGridi116647. 14 interactions.
IntActiQ8IZQ1. 15 interactions.
MINTiMINT-4829131.

Structurei

Secondary structure

1
3526
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3346 – 33483

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3WIMX-ray2.60B3341-3354[»]
ProteinModelPortaliQ8IZQ1.
SMRiQ8IZQ1. Positions 2611-2976, 3047-3245, 3409-3440, 3448-3513.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2683 – 2976294BEACHPROSITE-ProRule annotationAdd
BLAST
Repeati3077 – 311539WD 1Add
BLAST
Repeati3125 – 316440WD 2Add
BLAST
Repeati3167 – 320640WD 3Add
BLAST
Repeati3210 – 325445WD 4Add
BLAST
Repeati3408 – 344740WD 5Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2285 – 2981697Sufficient for translocalization to p62 bodies/ALISAdd
BLAST
Regioni2586 – 3526941Interaction with SQSTM1Add
BLAST
Regioni2981 – 3526546Interaction with ATG5Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi3344 – 33496LIR

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi472 – 4754Poly-Ser
Compositional biasi2516 – 25194Poly-Glu

Domaini

The LIR (LC3-interacting region) motif mediates the interaction with ATG8 family proteins.

Sequence similaritiesi

Contains 1 BEACH domain.PROSITE-ProRule annotation
Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation
Contains 5 WD repeats.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3454 – 351461FYVE-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, WD repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG236271.
GeneTreeiENSGT00760000119083.
HOVERGENiHBG094156.
InParanoidiQ8IZQ1.
OMAiQEEKLYM.
OrthoDBiEOG70CR5S.
PhylomeDBiQ8IZQ1.
TreeFamiTF313658.

Family and domain databases

Gene3Di1.10.1540.10. 1 hit.
1.25.10.10. 2 hits.
2.130.10.10. 2 hits.
2.30.29.40. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000409. BEACH_dom.
IPR013320. ConA-like_dom.
IPR023362. PH-BEACH_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF02138. Beach. 1 hit.
PF01363. FYVE. 1 hit.
PF14844. PH_BEACH. 1 hit.
PF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM01026. Beach. 1 hit.
SM00064. FYVE. 1 hit.
SM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
SSF49899. SSF49899. 1 hit.
SSF50978. SSF50978. 2 hits.
SSF57903. SSF57903. 1 hit.
SSF81837. SSF81837. 1 hit.
PROSITEiPS50197. BEACH. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8IZQ1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNMVKRIMGR PRQEECSPQD NALGLMHLRR LFTELCHPPR HMTQKEQEEK
60 70 80 90 100
LYMMLPVFNR VFGNAPPNTM TEKFSDLLQF TTQVSRLMVT EIRRRASNKS
110 120 130 140 150
TEAASRAIVQ FLEINQSEEA SRGWMLLTTI NLLASSGQKT VDCMTTMSVP
160 170 180 190 200
STLVKCLYLF FDLPHVPEAV GGAQNELPLA ERRGLLQKVF VQILVKLCSF
210 220 230 240 250
VSPAEELAQK DDLQLLFSAI TSWCPPYNLP WRKSAGEVLM TISRHGLSVN
260 270 280 290 300
VVKYIHEKEC LSTCVQNMQQ SDDLSPLEIV EMFAGLSCFL KDSSDVSQTL
310 320 330 340 350
LDDFRIWQGY NFLCDLLLRL EQAKEAESKD ALKDLVNLIT SLTTYGVSEL
360 370 380 390 400
KPAGITTGAP FLLPGFAVPQ PAGKGHSVRN VQAFAVLQNA FLKAKTSFLA
410 420 430 440 450
QIILDAITNI YMADNANYFI LESQHTLSQF AEKISKLPEV QNKYFEMLEF
460 470 480 490 500
VVFSLNYIPC KELISVSILL KSSSSYHCSI IAMKTLLKFT RHDYIFKDVF
510 520 530 540 550
REVGLLEVMV NLLHKYAALL KDPTQALNEQ GDSRNNSSVE DQKHLALLVM
560 570 580 590 600
ETLTVLLQGS NTNAGIFREF GGARCAHNIV KYPQCRQHAL MTIQQLVLSP
610 620 630 640 650
NGDDDMGTLL GLMHSAPPTE LQLKTDILRA LLSVLRESHR SRTVFRKVGG
660 670 680 690 700
FVYITSLLVA MERSLSCPPK NGWEKVNQNQ VFELLHTVFC TLTAAMRYEP
710 720 730 740 750
ANSHFFKTEI QYEKLADAVR FLGCFSDLRK ISAMNVFPSN TQPFQRLLEE
760 770 780 790 800
DVISIESVSP TLRHCSKLFI YLYKVATDSF DSRAEQIPPC LTSESSLPSP
810 820 830 840 850
WGTPALSRKR HAYHSVSTPP VYPPKNVADL KLHVTTSSLQ SSDAVIIHPG
860 870 880 890 900
AMLAMLDLLA SVGSVTQPEH ALDLQLAVAN ILQSLVHTER NQQVMCEAGL
910 920 930 940 950
HARLLQRCSA ALADEDHSLH PPLQRMFERL ASQALEPMVL REFLRLASPL
960 970 980 990 1000
NCGAWDKKLL KQYRVHKPSS LSYEPEMRSS MITSLEGLGT DNVFSLHEDN
1010 1020 1030 1040 1050
HYRISKSLVK SAEGSTVPLT RVKCLVSMTT PHDIRLHGSS VTPAFVEFDT
1060 1070 1080 1090 1100
SLEGFGCLFL PSLAPHNAPT NNTVTTGLID GAVVSGIGSG ERFFPPPSGL
1110 1120 1130 1140 1150
SYSSWFCIEH FSSPPNNHPV RLLTVVRRAN SSEQHYVCLA IVLSAKDRSL
1160 1170 1180 1190 1200
IVSTKEELLQ NYVDDFSEES SFYEILPCCA RFRCGELIIE GQWHHLVLVM
1210 1220 1230 1240 1250
SKGMLKNSTA ALYIDGQLVN TVKLHYVHST PGGSGSANPP VVSTVYAYIG
1260 1270 1280 1290 1300
TPPAQRQIAS LVWRLGPTHF LEEVLPSSNV TTIYELGPNY VGSFQAVCMP
1310 1320 1330 1340 1350
CKDAKSEGVV PSPVSLVPEE KVSFGLYALS VSSLTVARIR KVYNKLDSKA
1360 1370 1380 1390 1400
IAKQLGISSH ENATPVKLIH NSAGHLNGSA RTIGAALIGY LGVRTFVPKP
1410 1420 1430 1440 1450
VATTLQYVGG AAAILGLVAM ASDVEGLYAA VKALVCVVKS NPLASKEMER
1460 1470 1480 1490 1500
IKGYQLLAML LKKKRSLLNS HILHLTFSLV GTVDSGHETS IIPNSTAFQD
1510 1520 1530 1540 1550
LLCDFEVWLH APYELHLSLF EHFIELLTES SEASKNAKLM REFQLIPKLL
1560 1570 1580 1590 1600
LTLRDMSLSQ PTIAAISNVL SFLLQGFPSS NDLLRFGQFI SSTLPTFAVC
1610 1620 1630 1640 1650
EKFVVMEINN EEKLDTGTEE EFGGLVSANL ILLRNRLLDI LLKLIYTSKE
1660 1670 1680 1690 1700
KTSINLQACE ELVKTLGFDW IMMFMEEHLH STTVTAAMRI LVVLLSNQSI
1710 1720 1730 1740 1750
LIKFKEGLSG GGWLEQTDSV LTNKIGTVLG FNVGRSAGGR STVREINRDA
1760 1770 1780 1790 1800
CHFPGFPVLQ SFLPKHTNVP ALYFLLMALF LQQPVSELPE NLQVSVPVIS
1810 1820 1830 1840 1850
CRSKQGCQFD LDSIWTFIFG VPASSGTVVS SIHNVCTEAV FLLLGMLRSM
1860 1870 1880 1890 1900
LTSPWQSEEE GSWLREYPVT LMQFFRYLYH NVPDLASMWM SPDFLCALAA
1910 1920 1930 1940 1950
TVFPFNIRPY SEMVTDLDDE VGSPAEEFKA FAADTGMNRS QSEYCNVGTK
1960 1970 1980 1990 2000
TYLTNHPAKK FVFDFMRVLI IDNLCLTPAS KQTPLIDLLL EASPERSTRT
2010 2020 2030 2040 2050
QQKEFQTYIL DSVMDHLLAA DVLLGEDASL PITSGGSYQV LVNNVFYFTQ
2060 2070 2080 2090 2100
RVVDKLWQGM FNKESKLLID FIIQLIAQSK RRSQGLSLDA VYHCLNRTIL
2110 2120 2130 2140 2150
YQFSRAHKTV PQQVALLDSL RVLTVNRNLI LGPGNHDQEF ISCLAHCLIN
2160 2170 2180 2190 2200
LHVGSNVDGF GLEAEARMTT WHIMIPSDIE PDGSYSQDIS EGRQLLIKAV
2210 2220 2230 2240 2250
NRVWTELIHS KKQVLEELFK VTLPVNERGH VDIATARPLI EEAALKCWQN
2260 2270 2280 2290 2300
HLAHEKKCIS RGEALAPTTQ SKLSRVSSGF GLSKLTGSRR NRKESGLNKH
2310 2320 2330 2340 2350
SLSTQEISQW MFTHIAVVRD LVDTQYKEYQ ERQQNALKYV TEEWCQIECE
2360 2370 2380 2390 2400
LLRERGLWGP PIGSHLDKWM LEMTEGPCRM RKKMVRNDMF YNHYPYVPET
2410 2420 2430 2440 2450
EQETNVASEI PSKQPETPDD IPQKKPARYR RAVSYDSKEY YMRLASGNPA
2460 2470 2480 2490 2500
IVQDAIVESS EGEAAQQEPE HGEDTIAKVK GLVKPPLKRS RSAPDGGDEE
2510 2520 2530 2540 2550
NQEQLQDQIA EGSSIEEEEK TDNATLLRLL EEGEKIQHMY RCARVQGLDT
2560 2570 2580 2590 2600
SEGLLLFGKE HFYVIDGFTM TATREIRDIE TLPPNMHEPI IPRGARQGPS
2610 2620 2630 2640 2650
QLKRTCSIFA YEDIKEVHKR RYLLQPIAVE VFSGDGRNYL LAFQKGIRNK
2660 2670 2680 2690 2700
VYQRFLAVVP SLTDSSESVS GQRPNTSVEQ GSGLLSTLVG EKSVTQRWER
2710 2720 2730 2740 2750
GEISNFQYLM HLNTLAGRSY NDLMQYPVFP WILADYDSEE VDLTNPKTFR
2760 2770 2780 2790 2800
NLAKPMGAQT DERLAQYKKR YKDWEDPNGE TPAYHYGTHY SSAMIVASYL
2810 2820 2830 2840 2850
VRMEPFTQIF LRLQGGHFDL ADRMFHSVRE AWYSASKHNM ADVKELIPEF
2860 2870 2880 2890 2900
FYLPEFLFNS NNFDLGCKQN GTKLGDVILP PWAKGDPREF IRVHREALEC
2910 2920 2930 2940 2950
DYVSAHLHEW IDLIFGYKQQ GPAAVEAVNV FHHLFYEGQV DIYNINDPLK
2960 2970 2980 2990 3000
ETATIGFINN FGQIPKQLFK KPHPPKRVRS RLNGDNAGIS VLPGSTSDKI
3010 3020 3030 3040 3050
FFHHLDNLRP SLTPVKELKE PVGQIVCTDK GILAVEQNKV LIPPTWNKTF
3060 3070 3080 3090 3100
AWGYADLSCR LGTYESDKAM TVYECLSEWG QILCAICPNP KLVITGGTST
3110 3120 3130 3140 3150
VVCVWEMGTS KEKAKTVTLK QALLGHTDTV TCATASLAYH IIVSGSRDRT
3160 3170 3180 3190 3200
CIIWDLNKLS FLTQLRGHRA PVSALCINEL TGDIVSCAGT YIHVWSINGN
3210 3220 3230 3240 3250
PIVSVNTFTG RSQQIICCCM SEMNEWDTQN VIVTGHSDGV VRFWRMEFLQ
3260 3270 3280 3290 3300
VPETPAPEPA EVLEMQEDCP EAQIGQEAQD EDSSDSEADE QSISQDPKDT
3310 3320 3330 3340 3350
PSQPSSTSHR PRAASCRATA AWCTDSGSDD SRRWSDQLSL DEKDGFIFVN
3360 3370 3380 3390 3400
YSEGQTRAHL QGPLSHPHPN PIEVRNYSRL KPGYRWERQL VFRSKLTMHT
3410 3420 3430 3440 3450
AFDRKDNAHP AEVTALGISK DHSRILVGDS RGRVFSWSVS DQPGRSAADH
3460 3470 3480 3490 3500
WVKDEGGDSC SGCSVRFSLT ERRHHCRNCG QLFCQKCSRF QSEIKRLKIS
3510 3520
SPVRVCQNCY YNLQHERGSE DGPRNC
Length:3,526
Mass (Da):395,258
Last modified:June 27, 2006 - v2
Checksum:iF4D518E8C9C12E23
GO
Isoform 2 (identifier: Q8IZQ1-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     2408-2424: Missing.

Show »
Length:3,509
Mass (Da):393,367
Checksum:i5DF8D0918CD685F6
GO

Sequence cautioni

The sequence BAB71020.1 differs from that shown. Reason: Aberrant splicing.
The sequence AAH13377.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH15214.2 differs from that shown. Reason: Erroneous initiation.
The sequence BAC04455.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2944 – 29441N → T in AAN15137. (PubMed:15292400)Curated
Sequence conflicti3396 – 33961L → P in BAB71020. (PubMed:14702039)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti3032 – 30321I → V.
Corresponds to variant rs17368018 [ dbSNP | Ensembl ].
VAR_026864

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei2408 – 242417Missing in isoform 2. 1 PublicationVSP_019475Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF538685 mRNA. Translation: AAN15137.1.
AC095046 Genomic DNA. Translation: AAY40903.1.
AC104082 Genomic DNA. No translation available.
AB023210 mRNA. Translation: BAA76837.2.
AK055806 mRNA. Translation: BAB71020.1. Sequence problems.
AK094910 mRNA. Translation: BAC04455.1. Different initiation.
BC013377 mRNA. Translation: AAH13377.1. Different initiation.
BC015214 mRNA. Translation: AAH15214.2. Different initiation.
BC065502 mRNA. Translation: AAH65502.1.
BC119633 mRNA. Translation: AAI19634.1.
CCDSiCCDS3609.1. [Q8IZQ1-1]
RefSeqiNP_055806.2. NM_014991.4. [Q8IZQ1-1]
UniGeneiHs.480116.
Hs.726339.

Genome annotation databases

EnsembliENST00000295888; ENSP00000295888; ENSG00000163625. [Q8IZQ1-1]
GeneIDi23001.
KEGGihsa:23001.
UCSCiuc003hpd.3. human. [Q8IZQ1-1]

Polymorphism databases

DMDMi109896161.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF538685 mRNA. Translation: AAN15137.1 .
AC095046 Genomic DNA. Translation: AAY40903.1 .
AC104082 Genomic DNA. No translation available.
AB023210 mRNA. Translation: BAA76837.2 .
AK055806 mRNA. Translation: BAB71020.1 . Sequence problems.
AK094910 mRNA. Translation: BAC04455.1 . Different initiation.
BC013377 mRNA. Translation: AAH13377.1 . Different initiation.
BC015214 mRNA. Translation: AAH15214.2 . Different initiation.
BC065502 mRNA. Translation: AAH65502.1 .
BC119633 mRNA. Translation: AAI19634.1 .
CCDSi CCDS3609.1. [Q8IZQ1-1 ]
RefSeqi NP_055806.2. NM_014991.4. [Q8IZQ1-1 ]
UniGenei Hs.480116.
Hs.726339.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3WIM X-ray 2.60 B 3341-3354 [» ]
ProteinModelPortali Q8IZQ1.
SMRi Q8IZQ1. Positions 2611-2976, 3047-3245, 3409-3440, 3448-3513.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 116647. 14 interactions.
IntActi Q8IZQ1. 15 interactions.
MINTi MINT-4829131.

PTM databases

PhosphoSitei Q8IZQ1.

Polymorphism databases

DMDMi 109896161.

Proteomic databases

MaxQBi Q8IZQ1.
PaxDbi Q8IZQ1.
PRIDEi Q8IZQ1.

Protocols and materials databases

DNASUi 23001.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000295888 ; ENSP00000295888 ; ENSG00000163625 . [Q8IZQ1-1 ]
GeneIDi 23001.
KEGGi hsa:23001.
UCSCi uc003hpd.3. human. [Q8IZQ1-1 ]

Organism-specific databases

CTDi 23001.
GeneCardsi GC04M085590.
H-InvDB HIX0004348.
HGNCi HGNC:20751. WDFY3.
HPAi HPA042734.
HPA048572.
neXtProti NX_Q8IZQ1.
PharmGKBi PA134903706.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG236271.
GeneTreei ENSGT00760000119083.
HOVERGENi HBG094156.
InParanoidi Q8IZQ1.
OMAi QEEKLYM.
OrthoDBi EOG70CR5S.
PhylomeDBi Q8IZQ1.
TreeFami TF313658.

Enzyme and pathway databases

SignaLinki Q8IZQ1.

Miscellaneous databases

ChiTaRSi WDFY3. human.
GeneWikii WDFY3.
GenomeRNAii 23001.
NextBioi 43900.
PROi Q8IZQ1.

Gene expression databases

Bgeei Q8IZQ1.
CleanExi HS_WDFY3.
ExpressionAtlasi Q8IZQ1. baseline and differential.
Genevestigatori Q8IZQ1.

Family and domain databases

Gene3Di 1.10.1540.10. 1 hit.
1.25.10.10. 2 hits.
2.130.10.10. 2 hits.
2.30.29.40. 1 hit.
3.30.40.10. 1 hit.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000409. BEACH_dom.
IPR013320. ConA-like_dom.
IPR023362. PH-BEACH_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view ]
Pfami PF02138. Beach. 1 hit.
PF01363. FYVE. 1 hit.
PF14844. PH_BEACH. 1 hit.
PF00400. WD40. 2 hits.
[Graphical view ]
SMARTi SM01026. Beach. 1 hit.
SM00064. FYVE. 1 hit.
SM00320. WD40. 5 hits.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 3 hits.
SSF49899. SSF49899. 1 hit.
SSF50978. SSF50978. 2 hits.
SSF57903. SSF57903. 1 hit.
SSF81837. SSF81837. 1 hit.
PROSITEi PS50197. BEACH. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Alfy, a novel FYVE-domain-containing protein associated with protein granules and autophagic membranes."
    Simonsen A., Birkeland H.C.G., Gillooly D.J., Mizushima N., Kuma A., Yoshimori T., Slagsvold T., Brech A., Stenmark H.
    J. Cell Sci. 117:4239-4251(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Brain.
  2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 6:63-70(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1971-3526 (ISOFORM 1).
    Tissue: Brain.
  4. "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
    Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
    DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2381-3036 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2650-3526 (ISOFORM 1).
    Tissue: Corpus callosum and Kidney.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2749-3526 (ISOFORM 1).
    Tissue: Colon, Duodenum and Ovary.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "p62/SQSTM1 and ALFY interact to facilitate the formation of p62 bodies/ALIS and their degradation by autophagy."
    Clausen T.H., Lamark T., Isakson P., Finley K., Larsen K.B., Brech A., Overvatn A., Stenmark H., Bjorkoy G., Simonsen A., Johansen T.
    Autophagy 6:330-344(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SQSTM1, SUBCELLULAR LOCATION.
  9. "Functional interaction between sequestosome-1/p62 and autophagy-linked FYVE-containing protein WDFY3 in human osteoclasts."
    Hocking L.J., Mellis D.J., McCabe P.S., Helfrich M.H., Rogers M.J.
    Biochem. Biophys. Res. Commun. 402:543-548(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SQSTM1, TISSUE SPECIFICITY.
  10. "The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy."
    Filimonenko M., Isakson P., Finley K.D., Anderson M., Jeong H., Melia T.J., Bartlett B.J., Myers K.M., Birkeland H.C., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A.
    Mol. Cell 38:265-279(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ATG5, ASSOCIATION WITH THE ATG12-ATG5-ATG16L COMPLEX, SUBCELLULAR LOCATION.
  11. "TRAF6 mediates ubiquitination of KIF23/MKLP1 and is required for midbody ring degradation by selective autophagy."
    Isakson P., Lystad A.H., Breen K., Koster G., Stenmark H., Simonsen A.
    Autophagy 9:1955-1964(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Structural determinants in GABARAP required for the selective binding and recruitment of ALFY to LC3B-positive structures."
    Lystad A.H., Ichimura Y., Takagi K., Yang Y., Pankiv S., Kanegae Y., Kageyama S., Suzuki M., Saito I., Mizushima T., Komatsu M., Simonsen A.
    EMBO Rep. 15:557-565(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 3341-3354 IN COMPLEX WITH GABARAP, INTERACTION WITH MAP1LC3C; GABARAPL1 AND GABARAPL2, LIR MOTIF, MUTAGENESIS OF LYS-3343; ASP-3344; PHE-3346; ILE-3347; PHE-3348; VAL-3349 AND TYR-3351.

Entry informationi

Entry nameiWDFY3_HUMAN
AccessioniPrimary (citable) accession number: Q8IZQ1
Secondary accession number(s): Q4W5K5
, Q6P0Q5, Q8N1T2, Q8NAV6, Q96BS7, Q96D33, Q96N85, Q9Y2J7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 27, 2006
Last modified: October 29, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3