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Protein

Heparan-sulfate 6-O-sulfotransferase 3

Gene

HS6ST3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate.

Catalytic activityi

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 6-sulfate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei199SubstrateBy similarity1
Binding sitei204SubstrateBy similarity1
Active sitei209Proton acceptorBy similarity1
Binding sitei209SubstrateBy similarity1
Binding sitei245PAPSBy similarity1
Binding sitei253PAPSBy similarity1
Binding sitei257SubstrateBy similarity1
Binding sitei264SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15787.
ReactomeiR-HSA-2022928. HS-GAG biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Heparan-sulfate 6-O-sulfotransferase 3 (EC:2.8.2.-)
Short name:
HS6ST-3
Gene namesi
Name:HS6ST3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

HGNCiHGNC:19134. HS6ST3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4CytoplasmicSequence analysis4
Transmembranei5 – 27Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST23
Topological domaini28 – 471LumenalSequence analysisAdd BLAST444

GO - Cellular componenti

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi266722.
OpenTargetsiENSG00000185352.
PharmGKBiPA134879312.

Polymorphism and mutation databases

BioMutaiHS6ST3.
DMDMi116242508.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001908091 – 471Heparan-sulfate 6-O-sulfotransferase 3Add BLAST471

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi117N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi128N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi231N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi324N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi329N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi380N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8IZP7.
PaxDbiQ8IZP7.
PeptideAtlasiQ8IZP7.
PRIDEiQ8IZP7.

PTM databases

iPTMnetiQ8IZP7.
PhosphoSitePlusiQ8IZP7.

Expressioni

Gene expression databases

BgeeiENSG00000185352.
CleanExiHS_HS6ST3.
ExpressionAtlasiQ8IZP7. baseline and differential.
GenevisibleiQ8IZP7. HS.

Organism-specific databases

HPAiHPA051513.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000365895.

Structurei

3D structure databases

ProteinModelPortaliQ8IZP7.
SMRiQ8IZP7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni152 – 160PAPS bindingBy similarity9
Regioni182 – 183Substrate bindingBy similarity2
Regioni377 – 379PAPS bindingBy similarity3
Regioni383 – 384PAPS bindingBy similarity2

Sequence similaritiesi

Belongs to the sulfotransferase 6 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3955. Eukaryota.
ENOG410XSW4. LUCA.
GeneTreeiENSGT00390000013468.
HOGENOMiHOG000007772.
HOVERGENiHBG083012.
InParanoidiQ8IZP7.
KOiK08103.
OMAiTVTEDYN.
OrthoDBiEOG091G08UD.
PhylomeDBiQ8IZP7.
TreeFamiTF312835.

Family and domain databases

InterProiView protein in InterPro
IPR010635. Heparan_SO4-6-sulfoTrfase.
IPR027417. P-loop_NTPase.
IPR005331. Sulfotransferase.
PANTHERiPTHR12812. PTHR12812. 1 hit.
PfamiView protein in Pfam
PF03567. Sulfotransfer_2. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8IZP7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDERFNKWLL TPVLTLLFVV IMYQYVSPSC TSSCTNFGEQ PRAGEAGPPA
60 70 80 90 100
VPGPARRAQA PPEEWERRPQ LPPPPRGPPE GPRGAAAPEE EDEEPGDPRE
110 120 130 140 150
GEEEEEEDEP DPEAPENGSL PRFVPRFNFS LKDLTRFVDF NIKGRDVIVF
160 170 180 190 200
LHIQKTGGTT FGRHLVKNIR LEQPCSCKAG QKKCTCHRPG KKETWLFSRF
210 220 230 240 250
STGWSCGLHA DWTELTNCVP AIMEKKDCPR NHSHTRNFYY ITMLRDPVSR
260 270 280 290 300
YLSEWKHVQR GATWKTSLHM CDGRSPTPDE LPTCYPGDDW SGVSLREFMD
310 320 330 340 350
CTYNLANNRQ VRMLADLSLV GCYNLTFMNE SERNTILLQS AKNNLKNMAF
360 370 380 390 400
FGLTEFQRKT QFLFERTFNL KFISPFTQFN ITRASNVEIN EGARQRIEDL
410 420 430 440 450
NFLDMQLYEY AKDLFQQRYH HTKQLEHQRD RQKRREERRL QREHRDHQWP
460 470
KEDGAAEGTV TEDYNSQVVR W
Length:471
Mass (Da):54,844
Last modified:October 17, 2006 - v3
Checksum:i4A5F4DEA26FC7302
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37F → L in AAN33062 (Ref. 1) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_028159265K → R. Corresponds to variant dbSNP:rs9516771Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF539426 mRNA. Translation: AAN33062.1.
AL138816, AL158192 Genomic DNA. Translation: CAH71574.1.
AL158192, AL138816 Genomic DNA. Translation: CAI13709.1.
CR749677 mRNA. Translation: CAH18468.1.
CCDSiCCDS9481.1.
RefSeqiNP_703157.2. NM_153456.3.
UniGeneiHs.171001.
Hs.740393.

Genome annotation databases

EnsembliENST00000376705; ENSP00000365895; ENSG00000185352.
GeneIDi266722.
KEGGihsa:266722.
UCSCiuc001vmw.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiH6ST3_HUMAN
AccessioniPrimary (citable) accession number: Q8IZP7
Secondary accession number(s): Q5W0L0, Q68CW6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 17, 2006
Last modified: August 30, 2017
This is version 113 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families