Q8IZN3 (ZDH14_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable palmitoyltransferase ZDHHC14
EC=2.3.1.-

Alternative name(s):
NEW1 domain-containing protein
Short name=NEW1CP
Zinc finger DHHC domain-containing protein 14
Short name=DHHC-14

Gene names

Name:

ZDHHC14

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Palmitoyl-CoA + protein-cysteine = S-palmitoyl protein + CoA.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Domain	The DHHC domain is required for palmitoyltransferase activity By similarity.
Sequence similarities	Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily. Contains 1 DHHC-type zinc finger.
Sequence caution	The sequence BAB14941.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	transferase activity, transferring acyl groups Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8IZN3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8IZN3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     357-371: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Probable palmitoyltransferase ZDHHC14		PRO_0000212891
Regions
	Transmembrane	61 – 81	21	Helical; Potential
	Transmembrane	90 – 110	21	Helical; Potential
	Transmembrane	209 – 229	21	Helical; Potential
	Transmembrane	256 – 276	21	Helical; Potential
	Zinc finger	165 – 215	51	DHHC-type
Sites
	Active site	195	1	S-palmitoyl cysteine intermediate By similarity
Amino acid modifications
	Modified residue	455	1	Phosphoserine Ref.6 Ref.7
Natural variations
	Alternative sequence	357 – 371	15	Missing in isoform 2.		VSP_008651
	Natural variant	334	1	T → M. Corresponds to variant rs8180688 [ dbSNP | Ensembl ].		VAR_034586

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: B50CCDE1B738C135	FASTA	488	53,388
	10 20 30 40 50 60 MPPGGGGPMK DCEYSQISTH SSSPMESPHK KKKIAARRKW EVFPGRNKFF CNGRIMMARQ 70 80 90 100 110 120 TGVFYLTLVL ILVTSGLFFA FDCPYLAVKI TPAIPAVAGI LFFFVMGTLL RTSFSDPGVL 130 140 150 160 170 180 PRATPDEAAD LERQIDIANG TSSGGYRPPP RTKEVIINGQ TVKLKYCFTC KIFRPPRASH 190 200 210 220 230 240 CSLCDNCVER FDHHCPWVGN CVGKRNYRFF YMFILSLSFL TVFIFAFVIT HVILRSQQTG 250 260 270 280 290 300 FLNALKDSPA SVLEAVVCFF SVWSIVGLSG FHTYLISSNQ TTNEDIKGSW SNKRGKENYN 310 320 330 340 350 360 PYSYGNIFTN CCVALCGPIS PSLIDRRGYI QPDTPQPAAP SNGITMYGAT QSQSDMCDQD 370 380 390 400 410 420 QCIQSTKFVL QAAATPLLQS EPSLTSDELH LPGKPGLGTP CASLTLGPPT PPASMPNLAE 430 440 450 460 470 480 ATLADVMPRK DEHMGHQFLT PDEAPSPPRL LAAGSPLAHS RTMHVLGLAS QDSLHEDSVR GLVKLSSV
	Isoform 2 [UniParc]. Checksum: 534ACF9D9113B79E	FASTA	473	51,651
	10 20 30 40 50 60 MPPGGGGPMK DCEYSQISTH SSSPMESPHK KKKIAARRKW EVFPGRNKFF CNGRIMMARQ 70 80 90 100 110 120 TGVFYLTLVL ILVTSGLFFA FDCPYLAVKI TPAIPAVAGI LFFFVMGTLL RTSFSDPGVL 130 140 150 160 170 180 PRATPDEAAD LERQIDIANG TSSGGYRPPP RTKEVIINGQ TVKLKYCFTC KIFRPPRASH 190 200 210 220 230 240 CSLCDNCVER FDHHCPWVGN CVGKRNYRFF YMFILSLSFL TVFIFAFVIT HVILRSQQTG 250 260 270 280 290 300 FLNALKDSPA SVLEAVVCFF SVWSIVGLSG FHTYLISSNQ TTNEDIKGSW SNKRGKENYN 310 320 330 340 350 360 PYSYGNIFTN CCVALCGPIS PSLIDRRGYI QPDTPQPAAP SNGITMYGAT QSQSDMAAAT 370 380 390 400 410 420 PLLQSEPSLT SDELHLPGKP GLGTPCASLT LGPPTPPASM PNLAEATLAD VMPRKDEHMG 430 440 450 460 470 HQFLTPDEAP SPPRLLAAGS PLAHSRTMHV LGLASQDSLH EDSVRGLVKL SSV

References

[1]	Guo J.H., Chen L., Yu L. Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Stomach.
[2]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 233-488 (ISOFORM 1).
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 288-488 (ISOFORM 1). Tissue: Brain.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-455, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-455, MASS SPECTROMETRY. Tissue: Cervix carcinoma.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF542388 mRNA. Translation: AAN47142.1. AF542389 mRNA. Translation: AAN47143.1. AL450328, AL133510 Genomic DNA. Translation: CAI39698.1. AL133510, AL450328 Genomic DNA. Translation: CAI42852.1. AL450328, AL117344, AL133510 Genomic DNA. Translation: CAI39699.1. AL133510, AL117344, AL450328 Genomic DNA. Translation: CAI42853.1. AL117344, AL133510, AL450328 Genomic DNA. Translation: CAI20404.1. CH471051 Genomic DNA. Translation: EAW47671.1. AK024637 mRNA. Translation: BAB14941.1. Different initiation. BC056399 mRNA. Translation: AAH56399.1.
IPI	IPI00170691. IPI00413406.
RefSeq	NP_078906.2. NM_024630.2. NP_714968.1. NM_153746.1.
UniGene	Hs.187459.
3D structure databases
ProteinModelPortal	Q8IZN3.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000352821.
PTM databases
PhosphoSite	Q8IZN3.
Polymorphism databases
DMDM	37999849.
Proteomic databases
PaxDb	Q8IZN3.
PRIDE	Q8IZN3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000359775; ENSP00000352821; ENSG00000175048. ENST00000414563; ENSP00000410713; ENSG00000175048.
GeneID	79683.
KEGG	hsa:79683.
UCSC	uc003qqs.3. human. uc003qqt.3. human.
Organism-specific databases
CTD	79683.
GeneCards	GC06P157772.
HGNC	HGNC:20341. ZDHHC14.
HPA	HPA046033.
neXtProt	NX_Q8IZN3.
PharmGKB	PA134935513.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5273.
HOVERGEN	HBG056239.
InParanoid	Q8IZN3.
KO	K16675.
OrthoDB	EOG4G7BZ7.
PhylomeDB	Q8IZN3.
Gene expression databases
ArrayExpress	Q8IZN3.
Bgee	Q8IZN3.
CleanEx	HS_ZDHHC14.
Genevestigator	Q8IZN3.
GermOnline	ENSG00000175048. Homo sapiens.
Family and domain databases
InterPro	IPR001594. Znf_DHHC_palmitoyltrfase. [Graphical view]
Pfam	PF01529. zf-DHHC. 1 hit. [Graphical view]
PROSITE	PS50216. ZF_DHHC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ZDHHC14. human.
GenomeRNAi	79683.
NextBio	68938.

Entry information

Entry name

ZDH14_HUMAN

Accession

Primary (citable) accession number: Q8IZN3
Secondary accession number(s): A6NDB7 , Q5JS07, Q5JS08, Q6PHS4, Q8IZN2, Q9H7F1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 24, 2003
Last sequence update:	March 1, 2003
Last modified:	April 3, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.