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Q8IZL9 (CDK20_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase 20

EC=2.7.11.22
Alternative name(s):
CDK-activating kinase p42
Short name=CAK-kinase p42
Cell cycle-related kinase
Cell division protein kinase 20
Cyclin-dependent protein kinase H
Cyclin-kinase-activating kinase p42
Gene names
Name:CDK20
Synonyms:CCRK, CDCH
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for high-level Shh responses in the developing neural tube. Together with TBC1D32, controls the structure of the primary cilium by coordinating assembly of the ciliary membrane and axoneme, allowing GLI2 to be properly activated in response to SHH signaling By similarity. Involved in cell growth. Activates CDK2, a kinase involved in the control of the cell cycle, by phosphorylating residue 'Thr-160'. Ref.9

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Monomer. Interacts with TBC1D32 By similarity. Interacts with MAK. Ref.9 Ref.10

Subcellular location

Nucleus. Cytoplasm By similarity. Cell projectioncilium By similarity Ref.9.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence AAC98920.1 differs from that shown. Reason: Frameshift at position 331.

The sequence CAH72843.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8IZL9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8IZL9-2)

Also known as: Cardiac CCRK;

The sequence of this isoform differs from the canonical sequence as follows:
     168-243: WYRAPELLYG...LPDYNKISFK → SSLSCRTTTR...IHLSCRFLSV
     244-346: Missing.
Isoform 3 (identifier: Q8IZL9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     168-188: Missing.
     282-296: ALLHQYFFTAPLPAH → LPCLPIHLSCRFLSV
     297-346: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q8IZL9-4)

The sequence of this isoform differs from the canonical sequence as follows:
     25-25: E → EPRVGWQCLPSILQ
     168-188: Missing.
Note: No experimental confirmation available.
Isoform 5 (identifier: Q8IZL9-5)

The sequence of this isoform differs from the canonical sequence as follows:
     168-188: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 346346Cyclin-dependent kinase 20
PRO_0000085701

Regions

Domain4 – 288285Protein kinase
Nucleotide binding10 – 189ATP By similarity

Sites

Active site1271Proton acceptor By similarity
Binding site331ATP By similarity

Natural variations

Alternative sequence251E → EPRVGWQCLPSILQ in isoform 4.
VSP_043294
Alternative sequence168 – 24376WYRAP…KISFK → SSLSCRTTTRSPLRSRCPCP WRRCCLTSLPRHWICWVNSF STLLTSASQLPRLSSISTSS QLPCLPIHLSCRFLSV in isoform 2.
VSP_016748
Alternative sequence168 – 18821Missing in isoform 3, isoform 4 and isoform 5.
VSP_016749
Alternative sequence244 – 346103Missing in isoform 2.
VSP_016750
Alternative sequence282 – 29615ALLHQ…PLPAH → LPCLPIHLSCRFLSV in isoform 3.
VSP_016751
Alternative sequence297 – 34650Missing in isoform 3.
VSP_016752
Natural variant861S → L. Ref.4
Corresponds to variant rs28364953 [ dbSNP | Ensembl ].
VAR_024762
Natural variant1061S → N. Ref.11
Corresponds to variant rs41286029 [ dbSNP | Ensembl ].
VAR_041957
Natural variant1371A → T. Ref.4
Corresponds to variant rs28364955 [ dbSNP | Ensembl ].
VAR_024763
Natural variant2811K → R. Ref.4
Corresponds to variant rs28364963 [ dbSNP | Ensembl ].
VAR_024764

Experimental info

Mutagenesis1611T → A: Impairs CDK2 T-160 phosphorylation and activity. Ref.9
Sequence conflict301V → I in AAC98920. Ref.1
Sequence conflict301V → I in AAF43778. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: EB6A53E97301D5AE

FASTA34638,695
        10         20         30         40         50         60 
MDQYCILGRI GEGAHGIVFK AKHVETGEIV ALKKVALRRL EDGFPNQALR EIKALQEMED 

        70         80         90        100        110        120 
NQYVVQLKAV FPHGGGFVLA FEFMLSDLAE VVRHAQRPLA QAQVKSYLQM LLKGVAFCHA 

       130        140        150        160        170        180 
NNIVHRDLKP ANLLISASGQ LKIADFGLAR VFSPDGSRLY THQVATRWYR APELLYGARQ 

       190        200        210        220        230        240 
YDQGVDLWSV GCIMGELLNG SPLFPGKNDI EQLCYVLRIL GTPNPQVWPE LTELPDYNKI 

       250        260        270        280        290        300 
SFKEQVPMPL EEVLPDVSPQ ALDLLGQFLL YPPHQRIAAS KALLHQYFFT APLPAHPSEL 

       310        320        330        340 
PIPQRLGGPA PKAHPGPPHI HDFHVDRPLE ESLLNPELIR PFILEG 

« Hide

Isoform 2 (Cardiac CCRK) [UniParc].

Checksum: DA6A937563982609
Show »

FASTA24327,100
Isoform 3 [UniParc].

Checksum: 02CAB07F9B04C71C
Show »

FASTA27530,571
Isoform 4 [UniParc].

Checksum: 33F8C444B2AC77CC
Show »

FASTA33837,592
Isoform 5 [UniParc].

Checksum: D3C6C46BF34C152C
Show »

FASTA32536,113

References

« Hide 'large scale' references
[1]Jiang Y., Zhao K.
Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5).
[2]"Cloning a cyclin-dependent protein kinase CDCH."
Chen J.H., Luo W.Q., Hu S.N., Huang X.W., Tan X.Y., Yuan J.G., Qiang B.Q.
Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5).
[3]"Cardiac CCRK splice variant."
Qiu H., Depre C.
Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Heart.
[4]NIEHS SNPs program
Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS LEU-86; THR-137 AND ARG-281.
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4).
[6]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Uterus.
[9]"p42, a novel cyclin-dependent kinase-activating kinase in mammalian cells."
Liu Y., Wu C., Galaktionov K.
J. Biol. Chem. 279:4507-4514(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF THR-161.
[10]"Male germ cell-associated kinase is overexpressed in prostate cancer cells and causes mitotic defects via deregulation of APC/C(CDH1)."
Wang L.Y., Kung H.J.
Oncogene 31:2907-2918(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MAK.
[11]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] ASN-106.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF035013 mRNA. Translation: AAC98920.1. Frameshift.
AF113130 mRNA. Translation: AAF43778.1.
AY904367 mRNA. Translation: AAW82349.1.
AF547664 Genomic DNA. Translation: AAN28684.1.
AK075325 mRNA. Translation: BAG52110.1.
AK298993 mRNA. Translation: BAG61083.1.
AL353572 Genomic DNA. Translation: CAH72843.1. Sequence problems.
AL353572 Genomic DNA. Translation: CAM17657.1.
AL353572 Genomic DNA. Translation: CAM17658.1.
CH471089 Genomic DNA. Translation: EAW62746.1.
CH471089 Genomic DNA. Translation: EAW62748.1.
BC002655 mRNA. Translation: AAH02655.1.
RefSeqNP_001034892.1. NM_001039803.2.
NP_001164110.1. NM_001170639.1.
NP_001164111.1. NM_001170640.1.
NP_036251.2. NM_012119.4.
NP_848519.1. NM_178432.3.
UniGeneHs.522274.

3D structure databases

ProteinModelPortalQ8IZL9.
SMRQ8IZL9. Positions 1-334.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid117096. 31 interactions.
IntActQ8IZL9. 19 interactions.
STRING9606.ENSP00000322343.

Polymorphism databases

DMDM74759739.

Proteomic databases

PaxDbQ8IZL9.
PRIDEQ8IZL9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000325303; ENSP00000322343; ENSG00000156345. [Q8IZL9-1]
ENST00000336654; ENSP00000338975; ENSG00000156345. [Q8IZL9-4]
ENST00000375871; ENSP00000365031; ENSG00000156345. [Q8IZL9-2]
ENST00000375883; ENSP00000365043; ENSG00000156345. [Q8IZL9-5]
ENST00000605159; ENSP00000474485; ENSG00000156345. [Q8IZL9-3]
GeneID23552.
KEGGhsa:23552.
UCSCuc004apr.3. human. [Q8IZL9-1]
uc004apt.3. human. [Q8IZL9-4]
uc004apu.3. human. [Q8IZL9-2]
uc022bjj.1. human. [Q8IZL9-3]

Organism-specific databases

CTD23552.
GeneCardsGC09M090582.
HGNCHGNC:21420. CDK20.
HPAHPA007666.
HPA027379.
HPA027401.
MIM610076. gene.
neXtProtNX_Q8IZL9.
PharmGKBPA165585688.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
HOVERGENHBG014652.
KOK08817.
OMAAFTHGVP.
OrthoDBEOG75J0NH.
TreeFamTF327240.

Enzyme and pathway databases

SignaLinkQ8IZL9.

Gene expression databases

ArrayExpressQ8IZL9.
BgeeQ8IZL9.
CleanExHS_CCRK.
GenevestigatorQ8IZL9.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCDK20. human.
GenomeRNAi23552.
NextBio13603278.
PROQ8IZL9.
SOURCESearch...

Entry information

Entry nameCDK20_HUMAN
AccessionPrimary (citable) accession number: Q8IZL9
Secondary accession number(s): A2A389 expand/collapse secondary AC list , A2A390, B4DQX1, O95137, Q5EDC4, Q5VYW1, Q9BUF4
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2003
Last modified: March 19, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM