Q8IZL9 (CDK20_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase 20
EC=2.7.11.22

Alternative name(s):
CDK-activating kinase p42
Short name=CAK-kinase p42
Cell cycle-related kinase
Cell division protein kinase 20
Cyclin-dependent protein kinase H
Cyclin-kinase-activating kinase p42

Gene names

Name:	CDK20
Synonyms:	CCRK, CDCH

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for high-level Shh responses in the developing neural tube. Together with BROMI, controls the structure of the primary cilium by coordinating assembly of the ciliary membrane and axoneme, allowing GLI2 to be properly activated in response to SHH signaling By similarity. Involved in cell growth. Activates CDK2, a kinase involved in the control of the cell cycle, by phosphorylating residue 'Thr-160'. Ref.7
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Monomer. Interacts with BROMI By similarity. Interacts with MAK. Ref.7 Ref.8
Subcellular location	Nucleus. Cytoplasm By similarity. Cell projection › cilium By similarity Ref.7.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence CAH72843.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Cell projection Cilium Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cilium Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8IZL9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8IZL9-2)

Also known as: Cardiac CCRK;

The sequence of this isoform differs from the canonical sequence as follows:
     168-243: WYRAPELLYG...LPDYNKISFK → SSLSCRTTTR...IHLSCRFLSV
     244-346: Missing.

Isoform 3 (identifier: Q8IZL9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     168-188: Missing.
     282-296: ALLHQYFFTAPLPAH → LPCLPIHLSCRFLSV
     297-346: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q8IZL9-4)

The sequence of this isoform differs from the canonical sequence as follows:
     25-25: E → EPRVGWQCLPSILQ
     168-188: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Cyclin-dependent kinase 20		PRO_0000085701
Regions
	Domain	4 – 288	285	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity
Sites
	Active site	127	1	Proton acceptor By similarity
	Binding site	33	1	ATP By similarity
Natural variations
	Alternative sequence	25	1	E → EPRVGWQCLPSILQ in isoform 4.		VSP_043294
	Alternative sequence	168 – 243	76	WYRAP…KISFK → SSLSCRTTTRSPLRSRCPCP WRRCCLTSLPRHWICWVNSF STLLTSASQLPRLSSISTSS QLPCLPIHLSCRFLSV in isoform 2.		VSP_016748
	Alternative sequence	168 – 188	21	Missing in isoform 3 and isoform 4.		VSP_016749
	Alternative sequence	244 – 346	103	Missing in isoform 2.		VSP_016750
	Alternative sequence	282 – 296	15	ALLHQ…PLPAH → LPCLPIHLSCRFLSV in isoform 3.		VSP_016751
	Alternative sequence	297 – 346	50	Missing in isoform 3.		VSP_016752
	Natural variant	86	1	S → L. Ref.2 Corresponds to variant rs28364953 [ dbSNP | Ensembl ].		VAR_024762
	Natural variant	106	1	S → N. Ref.9 Corresponds to variant rs41286029 [ dbSNP | Ensembl ].		VAR_041957
	Natural variant	137	1	A → T. Ref.2 Corresponds to variant rs28364955 [ dbSNP | Ensembl ].		VAR_024763
	Natural variant	281	1	K → R. Ref.2 Corresponds to variant rs28364963 [ dbSNP | Ensembl ].		VAR_024764
Experimental info
	Mutagenesis	161	1	T → A: Impairs CDK2 T-160 phosphorylation and activity. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: EB6A53E97301D5AE	FASTA	346	38,695
	10 20 30 40 50 60 MDQYCILGRI GEGAHGIVFK AKHVETGEIV ALKKVALRRL EDGFPNQALR EIKALQEMED 70 80 90 100 110 120 NQYVVQLKAV FPHGGGFVLA FEFMLSDLAE VVRHAQRPLA QAQVKSYLQM LLKGVAFCHA 130 140 150 160 170 180 NNIVHRDLKP ANLLISASGQ LKIADFGLAR VFSPDGSRLY THQVATRWYR APELLYGARQ 190 200 210 220 230 240 YDQGVDLWSV GCIMGELLNG SPLFPGKNDI EQLCYVLRIL GTPNPQVWPE LTELPDYNKI 250 260 270 280 290 300 SFKEQVPMPL EEVLPDVSPQ ALDLLGQFLL YPPHQRIAAS KALLHQYFFT APLPAHPSEL 310 320 330 340 PIPQRLGGPA PKAHPGPPHI HDFHVDRPLE ESLLNPELIR PFILEG
	Isoform 2 (Cardiac CCRK) [UniParc]. Checksum: DA6A937563982609	FASTA	243	27,100
	10 20 30 40 50 60 MDQYCILGRI GEGAHGIVFK AKHVETGEIV ALKKVALRRL EDGFPNQALR EIKALQEMED 70 80 90 100 110 120 NQYVVQLKAV FPHGGGFVLA FEFMLSDLAE VVRHAQRPLA QAQVKSYLQM LLKGVAFCHA 130 140 150 160 170 180 NNIVHRDLKP ANLLISASGQ LKIADFGLAR VFSPDGSRLY THQVATRSSL SCRTTTRSPL 190 200 210 220 230 240 RSRCPCPWRR CCLTSLPRHW ICWVNSFSTL LTSASQLPRL SSISTSSQLP CLPIHLSCRF LSV
	Isoform 3 [UniParc]. Checksum: 02CAB07F9B04C71C	FASTA	275	30,571
	10 20 30 40 50 60 MDQYCILGRI GEGAHGIVFK AKHVETGEIV ALKKVALRRL EDGFPNQALR EIKALQEMED 70 80 90 100 110 120 NQYVVQLKAV FPHGGGFVLA FEFMLSDLAE VVRHAQRPLA QAQVKSYLQM LLKGVAFCHA 130 140 150 160 170 180 NNIVHRDLKP ANLLISASGQ LKIADFGLAR VFSPDGSRLY THQVATRSVG CIMGELLNGS 190 200 210 220 230 240 PLFPGKNDIE QLCYVLRILG TPNPQVWPEL TELPDYNKIS FKEQVPMPLE EVLPDVSPQA 250 260 270 LDLLGQFLLY PPHQRIAASK LPCLPIHLSC RFLSV
	Isoform 4 [UniParc]. Checksum: 33F8C444B2AC77CC	FASTA	338	37,592
	10 20 30 40 50 60 MDQYCILGRI GEGAHGIVFK AKHVEPRVGW QCLPSILQTG EIVALKKVAL RRLEDGFPNQ 70 80 90 100 110 120 ALREIKALQE MEDNQYVVQL KAVFPHGGGF VLAFEFMLSD LAEVVRHAQR PLAQAQVKSY 130 140 150 160 170 180 LQMLLKGVAF CHANNIVHRD LKPANLLISA SGQLKIADFG LARVFSPDGS RLYTHQVATR 190 200 210 220 230 240 SVGCIMGELL NGSPLFPGKN DIEQLCYVLR ILGTPNPQVW PELTELPDYN KISFKEQVPM 250 260 270 280 290 300 PLEEVLPDVS PQALDLLGQF LLYPPHQRIA ASKALLHQYF FTAPLPAHPS ELPIPQRLGG 310 320 330 PAPKAHPGPP HIHDFHVDRP LEESLLNPEL IRPFILEG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cardiac CCRK splice variant." Qiu H., Depre C. Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Heart.
[2]	NIEHS SNPs program Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS LEU-86; THR-137 AND ARG-281.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4).
[4]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Uterus.
[7]	"p42, a novel cyclin-dependent kinase-activating kinase in mammalian cells." Liu Y., Wu C., Galaktionov K. J. Biol. Chem. 279:4507-4514(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF THR-161.
[8]	"Male germ cell-associated kinase is overexpressed in prostate cancer cells and causes mitotic defects via deregulation of APC/C(CDH1)." Wang L.Y., Kung H.J. Oncogene 31:2907-2918(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MAK.
[9]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] ASN-106.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY904367 mRNA. Translation: AAW82349.1. AF547664 Genomic DNA. Translation: AAN28684.1. AK075325 mRNA. Translation: BAG52110.1. AK298993 mRNA. Translation: BAG61083.1. AL353572 Genomic DNA. Translation: CAH72843.1. Sequence problems. AL353572 Genomic DNA. Translation: CAM17657.1. AL353572 Genomic DNA. Translation: CAM17658.1. CH471089 Genomic DNA. Translation: EAW62746.1. CH471089 Genomic DNA. Translation: EAW62748.1. BC002655 mRNA. Translation: AAH02655.1.
IPI	IPI00218021. IPI00374861. IPI00554807. IPI00748335.
RefSeq	NP_001034892.1. NM_001039803.2. NP_001164110.1. NM_001170639.1. NP_001164111.1. NM_001170640.1. NP_036251.2. NM_012119.4. NP_848519.1. NM_178432.3.
UniGene	Hs.522274.
3D structure databases
ProteinModelPortal	Q8IZL9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8IZL9. 1 interaction.
STRING	9606.ENSP00000322343.
Polymorphism databases
DMDM	74759739.
Proteomic databases
PaxDb	Q8IZL9.
PRIDE	Q8IZL9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000325303; ENSP00000322343; ENSG00000156345. ENST00000336654; ENSP00000338975; ENSG00000156345. ENST00000375871; ENSP00000365031; ENSG00000156345. ENST00000375883; ENSP00000365043; ENSG00000156345.
GeneID	23552.
KEGG	hsa:23552.
UCSC	uc004apr.3. human. uc004apu.3. human. uc022bjj.1. human.
Organism-specific databases
CTD	23552.
GeneCards	GC09M090582.
HGNC	HGNC:21420. CDK20.
HPA	HPA007666. HPA027379. HPA027401.
MIM	610076. gene.
neXtProt	NX_Q8IZL9.
PharmGKB	PA165585688.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
HOVERGEN	HBG014652.
KO	K08817.
Gene expression databases
ArrayExpress	Q8IZL9.
Bgee	Q8IZL9.
CleanEx	HS_CCRK.
Genevestigator	Q8IZL9.
GermOnline	ENSG00000156345. Homo sapiens.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CDK20. human.
GenomeRNAi	23552.
NextBio	46104.
SOURCE	Search...

Entry information

Entry name

CDK20_HUMAN

Accession

Primary (citable) accession number: Q8IZL9
Secondary accession number(s): A2A389 , A2A390, B4DQX1, Q5EDC4, Q5VYW1, Q9BUF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.