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Q8IZJ3 (CPMD8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8
Gene names
Name:CPAMD8
Synonyms:KIAA1283
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1885 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Interacts with heparin. Ref.1

Subcellular location

Secreted Probable. Cell membrane; Peripheral membrane protein; Extracellular side Ref.1.

Tissue specificity

Highly expressed in the kidney, brain and testis and to a lower extent in heart, liver and small intestine. Ref.1

Induction

Up-regulated by IL1B/interleukin-1 beta and IL6/interleukin-6. Ref.1

Post-translational modification

Proteolytically cleaved into 2 chains of about 70 and 130 kDa. The fragments are not connected by disulfide bonds. Ref.1

Sequence similarities

Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. [View classification]

Contains 1 Kazal-like domain.

Sequence caution

The sequence BAC11400.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
Secreted
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSignal
   Molecular functionProtease inhibitor
Serine protease inhibitor
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular space

Inferred from electronic annotation. Source: InterPro

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type endopeptidase inhibitor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8IZJ3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8IZJ3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1809-1815: RVTAGPR → SQSGFSS
     1816-1885: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 18851860C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8
PRO_0000317695

Regions

Domain1706 – 175752Kazal-like
Compositional bias668 – 6714Poly-Arg
Compositional bias1242 – 12454Poly-Gln
Compositional bias1540 – 15434Poly-Asp
Compositional bias1765 – 17684Poly-Ser

Sites

Site671 – 6722Cleavage; by furin-like protease Probable

Amino acid modifications

Glycosylation1171N-linked (GlcNAc...) Potential
Glycosylation1901N-linked (GlcNAc...) Potential
Glycosylation2671N-linked (GlcNAc...) Potential
Glycosylation7571N-linked (GlcNAc...) Potential
Glycosylation14391N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1809 – 18157RVTAGPR → SQSGFSS in isoform 2.
VSP_031137
Alternative sequence1816 – 188570Missing in isoform 2.
VSP_031138
Natural variant2511R → W.
Corresponds to variant rs10426545 [ dbSNP | Ensembl ].
VAR_038655
Natural variant2651M → T.
Corresponds to variant rs4808551 [ dbSNP | Ensembl ].
VAR_038656
Natural variant2941R → Q. Ref.1 Ref.3
Corresponds to variant rs3745340 [ dbSNP | Ensembl ].
VAR_038657
Natural variant5391D → E.
Corresponds to variant rs3745335 [ dbSNP | Ensembl ].
VAR_038658
Natural variant5461H → R.
Corresponds to variant rs1824152 [ dbSNP | Ensembl ].
VAR_038659
Natural variant7361P → H.
Corresponds to variant rs9305083 [ dbSNP | Ensembl ].
VAR_038660
Natural variant11561V → I.
Corresponds to variant rs2250918 [ dbSNP | Ensembl ].
VAR_038661
Natural variant12681T → I. Ref.1 Ref.3
Corresponds to variant rs706761 [ dbSNP | Ensembl ].
VAR_038662
Natural variant18431Q → R.
Corresponds to variant rs1054533 [ dbSNP | Ensembl ].
VAR_038663

Experimental info

Sequence conflict15881S → G in BAC11400. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 22, 2009. Version 2.
Checksum: 5AA522DE6155C288

FASTA1,885206,702
        10         20         30         40         50         60 
MSGALLWPLL PLLLLLLSAR DGVRAAQPQA PGYLIAAPSV FRAGVEEVIS VTIFNSPREV 

        70         80         90        100        110        120 
TVQAQLVAQG EPVVQSQGAI LDKGTIKLKV PTGLRGQALL KVWGRGWQAE EGPLFHNQTS 

       130        140        150        160        170        180 
VTVDGRGASV FIQTDKPVYR PQHRVLISIF TVSPNLRPVN EKLEAYILDP RGSRMIEWRH 

       190        200        210        220        230        240 
LKPFCCGITN MSFPLSDQPV LGEWFIFVEM QGHAYNKSFE VQKYVLPKFE LLIDPPRYIQ 

       250        260        270        280        290        300 
DLDACETGTV RARYTFGKPV AGALMINMTV NGVGYYSHEV GRPVLRTTKI LGSRDFDICV 

       310        320        330        340        350        360 
RDMIPADVPE HFRGRVSIWA MVTSVDGSQQ VAFDDSTPVQ RQLVDIRYSK DTRKQFKPGL 

       370        380        390        400        410        420 
AYVGKVELSY PDGSPAEGVT VQIKAELTPK DNIYTSEVVS QRGLVGFEIP SIPTSAQHVW 

       430        440        450        460        470        480 
LETKVMALNG KPVGAQYLPS YLSLGSWYSP SQCYLQLQPP SHPLQVGEEA YFSVKSTCPC 

       490        500        510        520        530        540 
NFTLYYEVAA RGNIVLSGQQ PAHTTQQRSK RAAPALEKPI RLTHLSETEP PPAPEAEVDV 

       550        560        570        580        590        600 
CVTSLHLAVT PSMVPLGRLL VFYVRENGEG VADSLQFAVE TFFENQVSVT YSANETQPGE 

       610        620        630        640        650        660 
VVDLRIRAAR GSCVCVAAVD KSVYLLRSGF RLTPAQVFQE LEDYDVSDSF GVSREDGPFW 

       670        680        690        700        710        720 
WAGLTAQRRR RSSVFPWPWG ITKDSGFAFT ETGLVVMTDR VSLNHRQDGG LYTDEAVPAF 

       730        740        750        760        770        780 
QPHTGSLVAV APSRHPPRTE KRKRTFFPET WIWHCLNISD PSGEGTLSVK VPDSITSWVG 

       790        800        810        820        830        840 
EAVALSTSQG LGIAEPSLLK TFKPFFVDFM LPALIIRGEQ VKIPLSVYNY MGTCAEVYMK 

       850        860        870        880        890        900 
LSVPKGIQFV GHPGKRHVTK KMCVAPGEAE PIWVVLSFSD LGLNNITAKA LAYGDTNCCR 

       910        920        930        940        950        960 
DGRSSKHPEE NHADRRVPIG VDHVRRSVMV EAEGVPRAYT YSAFFCPSER VHISTPNKYE 

       970        980        990       1000       1010       1020 
FQYVQRPLRL TRFDVAVRAH NDARVALSSG PQDTAGMIEI VLGGHQNTRS WISTSKMGEP 

      1030       1040       1050       1060       1070       1080 
VASAHTAKIL SWDEFRTFWI SWRGGLIQVG HGPEPSNESV IVAWTLPRPP EVQFIGFSTG 

      1090       1100       1110       1120       1130       1140 
WGSMGEFRIW RKMEVDESYS EAFTLGVPHG AIPGSERATA SIIGDVMGPT LNHLNNLLRL 

      1150       1160       1170       1180       1190       1200 
PFGCGEQNMI HFAPNVFVLK YLQKTQQLSP EVERETTDYL VQGYQRQLTY KRQDGSYSAF 

      1210       1220       1230       1240       1250       1260 
GERDASGSMW LTAFVLKSFA QARSFIFVDP RELAAAKSWI IQQQQADGSF LAVGRVLNKD 

      1270       1280       1290       1300       1310       1320 
IQGGIHGTVP LTAYVVVALL ETGTASEEER GSTDKARHFL ESAAPLAMDP YSCALTTYAL 

      1330       1340       1350       1360       1370       1380 
TLLRSPAAPE ALRKLRSLAI MRDGVTHWSL SNSWDVDKGT FLSFSDRVSQ SVVSAEVEMT 

      1390       1400       1410       1420       1430       1440 
AYALLTYTLL GDVAAALPVV KWLSQQRNAL GGFSSTQDTC VALQALAEYA ILSYAGGINL 

      1450       1460       1470       1480       1490       1500 
TVSLASTNLD YQETFELHRT NQKVLQTAAI PSLPTGLFVS AKGDGCCLMQ IDVTYNVPDP 

      1510       1520       1530       1540       1550       1560 
VAKPAFQLLV SLQEPEAQGR PPPMPASAAE GSRGDWPPAD DDDPAADQHH QEYKVMLEVC 

      1570       1580       1590       1600       1610       1620 
TRWLHAGSSN MAVLEVPLLS GFRADIESLE QLLLDKHMGM KRYEVAGRRV LFYFDEIPSR 

      1630       1640       1650       1660       1670       1680 
CLTCVRFRAL RECVVGRTSA LPVSVYDYYE PAFEATRFYN VSTHSPLARE LCAGPACNEV 

      1690       1700       1710       1720       1730       1740 
ERAPARGPGW FPGESGPAVA PEEGAAIARC GCDHDCGAQG NPVCGSDGVV YASACRLREA 

      1750       1760       1770       1780       1790       1800 
ACRQAAPLEP APPSCCALEQ RLPASSSSTY GDDLASVAPG PLQQDVKLNG AGLEVEDSDP 

      1810       1820       1830       1840       1850       1860 
EPEGEAEDRV TAGPRPPVSS GNLESSTQSA SPFHRWGQTP APQRHSGRVV GAHRPGLLSP 

      1870       1880 
VFVYSPAFQS GGEEGLWMSN TCTLR 

« Hide

Isoform 2 [UniParc].

Checksum: E8F8D6DA1DECDB6D
Show »

FASTA1,815199,171

References

« Hide 'large scale' references
[1]"Identification and characterization of CPAMD8, a novel member of the complement 3/alpha2-macroglobulin family with a C-terminal Kazal domain."
Li Z.-F., Wu X.-H., Engvall E.
Genomics 83:1083-1093(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY, CLEAVAGE BY FURIN-LIKE PROTEASE, SUBCELLULAR LOCATION, INDUCTION BY IL1B AND IL6, INTERACTION WITH HEPARIN, VARIANTS GLN-294 AND ILE-1268.
[2]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Kikuno R., Hirosawa M., Nomura N., Ohara O.
DNA Res. 6:337-345(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-1885 (ISOFORM 1), VARIANTS GLN-294 AND ILE-1268.
Tissue: Brain.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1479-1885 (ISOFORM 2).
Tissue: Placenta.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY101765 mRNA. Translation: AAM50084.1.
AC008737 Genomic DNA. No translation available.
AC020908 Genomic DNA. No translation available.
AB033109 mRNA. Translation: BAA86597.1.
AK075099 mRNA. Translation: BAC11400.1. Different initiation.
RefSeqNP_056507.2. NM_015692.2.
UniGeneHs.631644.

3D structure databases

ProteinModelPortalQ8IZJ3.
SMRQ8IZJ3. Positions 30-1708, 1720-1756.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid118033. 3 interactions.
STRING9606.ENSP00000291440.

PTM databases

PhosphoSiteQ8IZJ3.

Polymorphism databases

DMDM259016204.

Proteomic databases

PaxDbQ8IZJ3.
PRIDEQ8IZJ3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000291440; ENSP00000291440; ENSG00000160111.
ENST00000443236; ENSP00000402505; ENSG00000160111.
GeneID27151.
KEGGhsa:27151.
UCSCuc002nfb.3. human. [Q8IZJ3-1]

Organism-specific databases

CTD27151.
GeneCardsGC19M017003.
H-InvDBHIX0014878.
HGNCHGNC:23228. CPAMD8.
HPAHPA031330.
MIM608841. gene.
neXtProtNX_Q8IZJ3.
PharmGKBPA134915057.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG241555.
HOGENOMHOG000168521.
HOVERGENHBG107753.
InParanoidQ8IZJ3.
OrthoDBEOG747PH3.
PhylomeDBQ8IZJ3.
TreeFamTF313285.

Gene expression databases

ArrayExpressQ8IZJ3.
BgeeQ8IZJ3.
CleanExHS_CPAMD8.
GenevestigatorQ8IZJ3.

Family and domain databases

Gene3D1.50.10.20. 1 hit.
2.60.40.690. 1 hit.
InterProIPR009048. A-macroglobulin_rcpt-bd.
IPR011626. A2M_comp.
IPR002890. A2M_N.
IPR011625. A2M_N_2.
IPR014756. Ig_E-set.
IPR002350. Kazal_dom.
IPR001599. Macroglobln_a2.
IPR019742. MacrogloblnA2_CS.
IPR019565. MacrogloblnA2_thiol-ester-bond.
IPR022041. Methyltransf_FA.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamPF00207. A2M. 1 hit.
PF07678. A2M_comp. 1 hit.
PF01835. A2M_N. 1 hit.
PF07703. A2M_N_2. 1 hit.
PF07677. A2M_recep. 1 hit.
PF07648. Kazal_2. 1 hit.
PF12248. Methyltransf_FA. 1 hit.
PF10569. Thiol-ester_cl. 1 hit.
[Graphical view]
SMARTSM00280. KAZAL. 1 hit.
[Graphical view]
SUPFAMSSF48239. SSF48239. 1 hit.
SSF49410. SSF49410. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEPS00477. ALPHA_2_MACROGLOBULIN. 1 hit.
PS51465. KAZAL_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi27151.
NextBio49911.
PROQ8IZJ3.
SOURCESearch...

Entry information

Entry nameCPMD8_HUMAN
AccessionPrimary (citable) accession number: Q8IZJ3
Secondary accession number(s): Q8NC09, Q9ULD7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: September 22, 2009
Last modified: April 16, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM