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Q8IZI9 (IFNL3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interferon lambda-3

Short name=IFN-lambda-3
Alternative name(s):
Cytokine Zcyto22
Interleukin-28B
Short name=IL-28B
Interleukin-28C
Short name=IL-28C
Gene names
Name:IFNL3
Synonyms:IL28B, IL28C, ZCYTO22
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length196 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Ref.1 Ref.2

Subcellular location

Secreted Ref.2.

Induction

By viral infections or double-stranded RNA. Ref.1 Ref.2

Sequence similarities

Belongs to the lambda interferon family.

Sequence caution

The sequence AAN28264.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Biological processAntiviral defense
   Cellular componentSecreted
   Coding sequence diversityPolymorphism
   DomainSignal
   Molecular functionCytokine
   PTMDisulfide bond
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdefense response to virus

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular space

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 196175Interferon lambda-3
PRO_0000015510

Amino acid modifications

Disulfide bond37 ↔ 136 Ref.6
Disulfide bond71 ↔ 169 Ref.6
Disulfide bond188 ↔ 195 Ref.6

Natural variations

Natural variant281R → H.
Corresponds to variant rs629976 [ dbSNP | Ensembl ].
VAR_063419
Natural variant701K → R. Ref.3
Corresponds to variant rs8103142 [ dbSNP | Ensembl ].
VAR_063420

Experimental info

Mutagenesis571K → A: 51 fold decrease in antiviral activity. Ref.6
Mutagenesis581D → A: 43 fold decrease in antiviral activity. Ref.6
Mutagenesis1181V → A: 68 fold decrease in antiviral activity. Ref.6
Mutagenesis1211Q → A: 46 fold decrease in antiviral activity. Ref.6
Mutagenesis1761F → A: 40 fold decrease in antiviral activity. Ref.6
Mutagenesis1791F → A: 650 fold decrease in antiviral activity. Ref.6
Sequence conflict1081T → S in AAN86127. Ref.2
Sequence conflict1201D → G in AAQ01561. Ref.3
Sequence conflict1551L → P in AAQ01561. Ref.3
Sequence conflict1561H → Y in AAN86127. Ref.2

Secondary structure

......................... 196
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8IZI9 [UniParc].

Last modified July 13, 2010. Version 2.
Checksum: F2322E10C887184B

FASTA19621,706
        10         20         30         40         50         60 
MTGDCMPVLV LMAAVLTVTG AVPVARLRGA LPDARGCHIA QFKSLSPQEL QAFKRAKDAL 

        70         80         90        100        110        120 
EESLLLKDCK CRSRLFPRTW DLRQLQVRER PVALEAELAL TLKVLEATAD TDPALGDVLD 

       130        140        150        160        170        180 
QPLHTLHHIL SQLRACIQPQ PTAGPRTRGR LHHWLHRLQE APKKESPGCL EASVTFNLFR 

       190 
LLTRDLNCVA SGDLCV 

« Hide

References

« Hide 'large scale' references
[1]"IL-28, IL-29 and their class II cytokine receptor IL-28R."
Sheppard P., Kindsvogel W., Xu W., Henderson K., Schlutsmeyer S., Whitmore T.E., Kuestner R., Garrigues U., Birks C., Roraback J., Ostrander C., Dong D., Shin J., Presnell S., Fox B., Haldeman B., Cooper E., Taft D. expand/collapse author list , Gilbert T., Grant F.J., Tackett M., Krivan W., McKnight G., Clegg C., Foster D., Klucher K.M.
Nat. Immunol. 4:63-68(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
[2]"IFN-lambdas mediate antiviral protection through a distinct class II cytokine receptor complex."
Kotenko S.V., Gallagher G., Baurin V.V., Lewis-Antes A., Shen M., Shah N.K., Langer J.A., Sheikh F., Dickensheets H., Donnelly R.P.
Nat. Immunol. 4:69-77(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
[3]"Liposome-mediated IL-28 and IL-29 expression in A549 cells and anti-viral effect of IL-28 and IL-29 on WISH cells."
Li M.C., Wang H.Y., Wang H.Y., Li T., He S.H.
Acta Pharmacol. Sin. 27:453-459(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-70.
Tissue: Peripheral blood leukocyte.
[4]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Interferon-lambda is functionally an interferon but structurally related to the interleukin-10 family."
Gad H.H., Dellgren C., Hamming O.J., Vends S., Paludan S.R., Hartmann R.
J. Biol. Chem. 284:20869-20875(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS), DISULFIDE BONDS, MUTAGENESIS OF LYS-57; ASP-58; VAL-118; GLN-121; PHE-176 AND PHE-179.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY129149 mRNA. Translation: AAN28264.1. Different initiation.
AY184374 mRNA. Translation: AAN86127.1.
AY336714 mRNA. Translation: AAR24509.1.
AY336717 mRNA. Translation: AAQ01561.1.
AC011445 Genomic DNA. No translation available.
BC130314 mRNA. Translation: AAI30315.1.
BC130316 mRNA. Translation: AAI30317.1.
CCDSCCDS12530.1.
RefSeqNP_742151.2. NM_172139.2.
XP_005258822.1. XM_005258765.2.
UniGeneHs.406744.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3HHCX-ray2.80A/B/C/D1-196[»]
ProteinModelPortalQ8IZI9.
SMRQ8IZI9. Positions 37-196.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000347165.

Polymorphism databases

DMDM300669648.

Proteomic databases

PaxDbQ8IZI9.
PRIDEQ8IZI9.

Protocols and materials databases

DNASU282617.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000413851; ENSP00000409000; ENSG00000197110.
GeneID282617.
KEGGhsa:282617.
UCSCuc010xut.2. human.

Organism-specific databases

CTD282617.
GeneCardsGC19M039735.
HGNCHGNC:18365. IFNL3.
MIM607402. gene.
neXtProtNX_Q8IZI9.
Orphanet284102. Response to polyethylene glycol/interferon-ribavirin in hepatitis C.
PharmGKBPA134952671.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG40189.
HOGENOMHOG000232074.
HOVERGENHBG052108.
InParanoidQ8IZI9.
KOK05447.
OMARTWDLQQ.
OrthoDBEOG7F24V6.
PhylomeDBQ8IZI9.
TreeFamTF336172.

Enzyme and pathway databases

SignaLinkQ8IZI9.

Gene expression databases

CleanExHS_IL28B.
GenevestigatorQ8IZI9.

Family and domain databases

InterProIPR029177. INF_lambda.
[Graphical view]
PfamPF15177. IL28A. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ8IZI9.
GeneWikiInterleukin_28B.
GenomeRNAi282617.
NextBio93470.
PROQ8IZI9.
SOURCESearch...

Entry information

Entry nameIFNL3_HUMAN
AccessionPrimary (citable) accession number: Q8IZI9
Secondary accession number(s): A2BDE1 expand/collapse secondary AC list , Q6VN56, Q7Z4J3, Q8IWL6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: July 13, 2010
Last modified: July 9, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM