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Protein

Adhesion G protein-coupled receptor F5

Gene

ADGRF5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor that plays a critical role in lung surfactant homeostasis. May play a role in controlling adipocyte function.By similarity

GO - Molecular functioni

  • G-protein coupled receptor activity Source: GDB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-HSA-5683826. Surfactant metabolism.

Protein family/group databases

MEROPSiP02.019.

Names & Taxonomyi

Protein namesi
Recommended name:
Adhesion G protein-coupled receptor F51 Publication
Alternative name(s):
G-protein coupled receptor 116
Gene namesi
Name:ADGRF5Imported
Synonyms:GPR116, KIAA0758
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:19030. ADGRF5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 1006ExtracellularCuratedAdd BLAST985
Transmembranei1007 – 1027Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini1028 – 1053CytoplasmicCuratedAdd BLAST26
Transmembranei1054 – 1074Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini1075 – 1090ExtracellularCuratedAdd BLAST16
Transmembranei1091 – 1111Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini1112 – 1128CytoplasmicCuratedAdd BLAST17
Transmembranei1129 – 1149Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini1150 – 1173ExtracellularCuratedAdd BLAST24
Transmembranei1174 – 1194Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini1195 – 1220CytoplasmicCuratedAdd BLAST26
Transmembranei1221 – 1241Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini1242 – 1244ExtracellularCurated3
Transmembranei1245 – 1265Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini1266 – 1346CytoplasmicCuratedAdd BLAST81

GO - Cellular componenti

  • apical part of cell Source: Ensembl
  • cell surface Source: MGI
  • cytoplasmic vesicle Source: MGI
  • integral component of membrane Source: GDB
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi221395.
OpenTargetsiENSG00000069122.
PharmGKBiPA134886165.

Polymorphism and mutation databases

BioMutaiGPR116.
DMDMi229462973.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000001289622 – 1346Adhesion G protein-coupled receptor F5Add BLAST1325

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi73N-linked (GlcNAc...)Sequence analysis1
Glycosylationi94N-linked (GlcNAc...)Sequence analysis1
Glycosylationi106N-linked (GlcNAc...)Sequence analysis1
Glycosylationi188N-linked (GlcNAc...)Sequence analysis1
Glycosylationi256N-linked (GlcNAc...)1 Publication1
Glycosylationi272N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi293 ↔ 350PROSITE-ProRule annotation
Glycosylationi301N-linked (GlcNAc...)Sequence analysis1
Glycosylationi315N-linked (GlcNAc...)1 Publication1
Glycosylationi328N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi391 ↔ 449PROSITE-ProRule annotation
Glycosylationi398N-linked (GlcNAc...)Sequence analysis1
Glycosylationi472N-linked (GlcNAc...)Sequence analysis1
Glycosylationi487N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi492 ↔ 545PROSITE-ProRule annotation
Glycosylationi505N-linked (GlcNAc...)Sequence analysis1
Glycosylationi540N-linked (GlcNAc...)Sequence analysis1
Glycosylationi627N-linked (GlcNAc...)Sequence analysis1
Glycosylationi649N-linked (GlcNAc...)Sequence analysis1
Glycosylationi666N-linked (GlcNAc...)Sequence analysis1
Glycosylationi820N-linked (GlcNAc...)Sequence analysis1
Glycosylationi931N-linked (GlcNAc...)Sequence analysis1
Glycosylationi963N-linked (GlcNAc...)Sequence analysis1
Glycosylationi982N-linked (GlcNAc...)Sequence analysis1
Modified residuei1300PhosphothreonineBy similarity1
Modified residuei1307PhosphoserineBy similarity1

Post-translational modificationi

Proteolytically cleaved at multiple sites: one in the GPS domain (S1 site) and the other in the SEA domain (S2 site). The proteolytic cleavage at S1 site generates an extracellular subunit and a seven-transmembrane subunit. The proteolytic cleavage at S2 site generates a fragment that undergoes proteolytic cleavage by the processing enzyme furin.By similarity
Highly glycosylated.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei51 – 52Cleavage; by furinBy similarity2
Sitei226 – 227CleavageBy similarity2
Sitei990 – 991Cleavage; by autolysisBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8IZF2.
PeptideAtlasiQ8IZF2.
PRIDEiQ8IZF2.

PTM databases

iPTMnetiQ8IZF2.
PhosphoSitePlusiQ8IZF2.

Expressioni

Gene expression databases

BgeeiENSG00000069122.
CleanExiHS_GPR116.
GenevisibleiQ8IZF2. HS.

Organism-specific databases

HPAiCAB009861.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Heterodimer of 2 chains generated by proteolytic processing; the large extracellular N-terminal fragment and the membrane-bound C-terminal fragment predominantly remain associated and non-covalently linked. Fragment generates by the processing enzyme furin remains attached to the extracellular N-terminal fragment. Interacts (via N-terminal extracellular domain) with SFTPD.By similarity

Protein-protein interaction databases

IntActiQ8IZF2. 1 interactor.
MINTiMINT-4715450.
STRINGi9606.ENSP00000265417.

Structurei

3D structure databases

ProteinModelPortaliQ8IZF2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini166 – 273SEAPROSITE-ProRule annotationAdd BLAST108
Domaini267 – 368Ig-like 1Add BLAST102
Domaini369 – 466Ig-like 2Add BLAST98
Domaini471 – 561Ig-like 3Add BLAST91
Domaini951 – 1002GPSPROSITE-ProRule annotationAdd BLAST52

Sequence similaritiesi

Contains 1 GPS domain.PROSITE-ProRule annotation
Contains 1 SEA domain.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4193. Eukaryota.
ENOG410XSD2. LUCA.
GeneTreeiENSGT00830000128272.
HOGENOMiHOG000112764.
HOVERGENiHBG051772.
InParanoidiQ8IZF2.
KOiK08458.
OMAiGWDSSGC.
OrthoDBiEOG091G00IU.
PhylomeDBiQ8IZF2.
TreeFamiTF316380.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
3.30.70.960. 1 hit.
InterProiIPR017981. GPCR_2-like.
IPR008078. GPCR_2_Ig-hepta-like_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR000082. SEA_dom.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF01825. GPS. 1 hit.
PF01390. SEA. 1 hit.
[Graphical view]
PRINTSiPR00249. GPCRSECRETIN.
PR01695. IGHEPTARCPTR.
SMARTiSM00303. GPS. 1 hit.
SM00409. IG. 2 hits.
SM00408. IGc2. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
SSF82671. SSF82671. 1 hit.
PROSITEiPS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
PS50835. IG_LIKE. 3 hits.
PS50024. SEA. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8IZF2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKSPRRTTLC LMFIVIYSSK AALNWNYEST IHPLSLHEHE PAGEEALRQK
60 70 80 90 100
RAVATKSPTA EEYTVNIEIS FENASFLDPI KAYLNSLSFP IHGNNTDQIT
110 120 130 140 150
DILSINVTTV CRPAGNEIWC SCETGYGWPR ERCLHNLICQ ERDVFLPGHH
160 170 180 190 200
CSCLKELPPN GPFCLLQEDV TLNMRVRLNV GFQEDLMNTS SALYRSYKTD
210 220 230 240 250
LETAFRKGYG ILPGFKGVTV TGFKSGSVVV TYEVKTTPPS LELIHKANEQ
260 270 280 290 300
VVQSLNQTYK MDYNSFQAVT INESNFFVTP EIIFEGDTVS LVCEKEVLSS
310 320 330 340 350
NVSWRYEEQQ LEIQNSSRFS IYTALFNNMT SVSKLTIHNI TPGDAGEYVC
360 370 380 390 400
KLILDIFEYE CKKKIDVMPI QILANEEMKV MCDNNPVSLN CCSQGNVNWS
410 420 430 440 450
KVEWKQEGKI NIPGTPETDI DSSCSRYTLK ADGTQCPSGS SGTTVIYTCE
460 470 480 490 500
FISAYGARGS ANIKVTFISV ANLTITPDPI SVSEGQNFSI KCISDVSNYD
510 520 530 540 550
EVYWNTSAGI KIYQRFYTTR RYLDGAESVL TVKTSTREWN GTYHCIFRYK
560 570 580 590 600
NSYSIATKDV IVHPLPLKLN IMVDPLEATV SCSGSHHIKC CIEEDGDYKV
610 620 630 640 650
TFHTGSSSLP AAKEVNKKQV CYKHNFNASS VSWCSKTVDV CCHFTNAANN
660 670 680 690 700
SVWSPSMKLN LVPGENITCQ DPVIGVGEPG KVIQKLCRFS NVPSSPESPI
710 720 730 740 750
GGTITYKCVG SQWEEKRNDC ISAPINSLLQ MAKALIKSPS QDEMLPTYLK
760 770 780 790 800
DLSISIDKAE HEISSSPGSL GAIINILDLL STVPTQVNSE MMTHVLSTVN
810 820 830 840 850
VILGKPVLNT WKVLQQQWTN QSSQLLHSVE RFSQALQSGD SPPLSFSQTN
860 870 880 890 900
VQMSSMVIKS SHPETYQQRF VFPYFDLWGN VVIDKSYLEN LQSDSSIVTM
910 920 930 940 950
AFPTLQAILA QDIQENNFAE SLVMTTTVSH NTTMPFRISM TFKNNSPSGG
960 970 980 990 1000
ETKCVFWNFR LANNTGGWDS SGCYVEEGDG DNVTCICDHL TSFSILMSPD
1010 1020 1030 1040 1050
SPDPSSLLGI LLDIISYVGV GFSILSLAAC LVVEAVVWKS VTKNRTSYMR
1060 1070 1080 1090 1100
HTCIVNIAAS LLVANTWFIV VAAIQDNRYI LCKTACVAAT FFIHFFYLSV
1110 1120 1130 1140 1150
FFWMLTLGLM LFYRLVFILH ETSRSTQKAI AFCLGYGCPL AISVITLGAT
1160 1170 1180 1190 1200
QPREVYTRKN VCWLNWEDTK ALLAFAIPAL IIVVVNITIT IVVITKILRP
1210 1220 1230 1240 1250
SIGDKPCKQE KSSLFQISKS IGVLTPLLGL TWGFGLTTVF PGTNLVFHII
1260 1270 1280 1290 1300
FAILNVFQGL FILLFGCLWD LKVQEALLNK FSLSRWSSQH SKSTSLGSST
1310 1320 1330 1340
PVFSMSSPIS RRFNNLFGKT GTYNVSTPEA TSSSLENSSS ASSLLN
Length:1,346
Mass (Da):149,457
Last modified:May 5, 2009 - v3
Checksum:i72A9D02B08218A60
GO
Isoform 2 (identifier: Q8IZF2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1273-1292: Missing.
     1322-1346: Missing.

Show »
Length:1,301
Mass (Da):144,601
Checksum:i9DEB380A0BEDA44B
GO
Isoform 3 (identifier: Q8IZF2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     272-414: NESNFFVTPE...KQEGKINIPG → R

Note: No experimental confirmation available.
Show »
Length:1,204
Mass (Da):133,307
Checksum:i0106B9A59C2D5B0E
GO

Sequence cautioni

The sequence BAA34478 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAB43394 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti272Missing in AAN46672 (PubMed:12435584).Curated1
Sequence conflicti573V → I in CAB43394 (PubMed:15489334).Curated1
Sequence conflicti1075Q → R in AL832125 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_025326604T → M.4 PublicationsCorresponds to variant rs586024dbSNPEnsembl.1
Natural variantiVAR_055291801V → I.1 PublicationCorresponds to variant rs9395218dbSNPEnsembl.1
Natural variantiVAR_024477856M → T.2 PublicationsCorresponds to variant rs547499dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_039130272 – 414NESNF…INIPG → R in isoform 3. 1 PublicationAdd BLAST143
Alternative sequenceiVSP_0108371273 – 1292Missing in isoform 2. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_0108381322 – 1346Missing in isoform 2. 1 PublicationAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY140958 mRNA. Translation: AAN46672.1.
AY498875 mRNA. Translation: AAS21061.1.
AB018301 mRNA. Translation: BAA34478.2. Different initiation.
AL050295 mRNA. Translation: CAB43394.1. Sequence problems.
AL832125 mRNA. No translation available.
AL096772 Genomic DNA. Translation: CAB61578.1.
BC066121 mRNA. Translation: AAH66121.1.
AY255552 mRNA. Translation: AAO85064.1.
CCDSiCCDS4919.1. [Q8IZF2-1]
PIRiT08685.
RefSeqiNP_001091988.1. NM_001098518.1. [Q8IZF2-1]
NP_056049.4. NM_015234.4. [Q8IZF2-1]
UniGeneiHs.362806.

Genome annotation databases

EnsembliENST00000265417; ENSP00000265417; ENSG00000069122. [Q8IZF2-1]
ENST00000283296; ENSP00000283296; ENSG00000069122. [Q8IZF2-1]
GeneIDi221395.
KEGGihsa:221395.
UCSCiuc003oyo.5. human. [Q8IZF2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY140958 mRNA. Translation: AAN46672.1.
AY498875 mRNA. Translation: AAS21061.1.
AB018301 mRNA. Translation: BAA34478.2. Different initiation.
AL050295 mRNA. Translation: CAB43394.1. Sequence problems.
AL832125 mRNA. No translation available.
AL096772 Genomic DNA. Translation: CAB61578.1.
BC066121 mRNA. Translation: AAH66121.1.
AY255552 mRNA. Translation: AAO85064.1.
CCDSiCCDS4919.1. [Q8IZF2-1]
PIRiT08685.
RefSeqiNP_001091988.1. NM_001098518.1. [Q8IZF2-1]
NP_056049.4. NM_015234.4. [Q8IZF2-1]
UniGeneiHs.362806.

3D structure databases

ProteinModelPortaliQ8IZF2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8IZF2. 1 interactor.
MINTiMINT-4715450.
STRINGi9606.ENSP00000265417.

Protein family/group databases

MEROPSiP02.019.
GPCRDBiSearch...

PTM databases

iPTMnetiQ8IZF2.
PhosphoSitePlusiQ8IZF2.

Polymorphism and mutation databases

BioMutaiGPR116.
DMDMi229462973.

Proteomic databases

PaxDbiQ8IZF2.
PeptideAtlasiQ8IZF2.
PRIDEiQ8IZF2.

Protocols and materials databases

DNASUi221395.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000265417; ENSP00000265417; ENSG00000069122. [Q8IZF2-1]
ENST00000283296; ENSP00000283296; ENSG00000069122. [Q8IZF2-1]
GeneIDi221395.
KEGGihsa:221395.
UCSCiuc003oyo.5. human. [Q8IZF2-1]

Organism-specific databases

CTDi221395.
DisGeNETi221395.
GeneCardsiADGRF5.
HGNCiHGNC:19030. ADGRF5.
HPAiCAB009861.
neXtProtiNX_Q8IZF2.
OpenTargetsiENSG00000069122.
PharmGKBiPA134886165.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4193. Eukaryota.
ENOG410XSD2. LUCA.
GeneTreeiENSGT00830000128272.
HOGENOMiHOG000112764.
HOVERGENiHBG051772.
InParanoidiQ8IZF2.
KOiK08458.
OMAiGWDSSGC.
OrthoDBiEOG091G00IU.
PhylomeDBiQ8IZF2.
TreeFamiTF316380.

Enzyme and pathway databases

ReactomeiR-HSA-5683826. Surfactant metabolism.

Miscellaneous databases

ChiTaRSiGPR116. human.
GeneWikiiGPR116.
GenomeRNAii221395.
PROiQ8IZF2.

Gene expression databases

BgeeiENSG00000069122.
CleanExiHS_GPR116.
GenevisibleiQ8IZF2. HS.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
3.30.70.960. 1 hit.
InterProiIPR017981. GPCR_2-like.
IPR008078. GPCR_2_Ig-hepta-like_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR000082. SEA_dom.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF01825. GPS. 1 hit.
PF01390. SEA. 1 hit.
[Graphical view]
PRINTSiPR00249. GPCRSECRETIN.
PR01695. IGHEPTARCPTR.
SMARTiSM00303. GPS. 1 hit.
SM00409. IG. 2 hits.
SM00408. IGc2. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
SSF82671. SSF82671. 1 hit.
PROSITEiPS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
PS50835. IG_LIKE. 3 hits.
PS50024. SEA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGRF5_HUMAN
AccessioniPrimary (citable) accession number: Q8IZF2
Secondary accession number(s): O94858
, Q5TF06, Q6RGN2, Q86SP0, Q9Y3Z2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: May 5, 2009
Last modified: November 30, 2016
This is version 142 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.