Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q8IZE3 (PACE1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein-associating with the carboxyl-terminal domain of ezrin
Alternative name(s):
Ezrin-binding protein PACE-1
SCY1-like protein 3
Gene names
Name:SCYL3
Synonyms:PACE1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length742 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a role in regulating cell adhesion/migration complexes in migrating cells. Ref.1

Subunit structure

Interacts with EZR/VIL2 C-terminal domain. Ref.1

Subcellular location

Cytoplasm. Golgi apparatus. Cell projectionlamellipodium. Note: Colocalized with EZR/VIL2, actin and CD44 in lamellipodia. Ref.1

Tissue specificity

Ubiquitously expressed. Ref.1

Domain

The protein kinase domain is predicted to be catalytically inactive.

Post-translational modification

May be myristoylated; myristoylation may target it to Golgi compartment.

Phosphorylated. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily.

Contains 4 HEAT repeats.

Contains 1 protein kinase domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

EZRP153114EBI-1380680,EBI-1056902

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8IZE3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8IZE3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     438-491: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 742742Protein-associating with the carboxyl-terminal domain of ezrin
PRO_0000058167

Regions

Domain2 – 245244Protein kinase
Repeat199 – 23840HEAT 1
Repeat285 – 32339HEAT 2
Repeat333 – 37038HEAT 3
Repeat372 – 40938HEAT 4
Region548 – 742195Interaction with EZR

Amino acid modifications

Modified residue4761Phosphoserine By similarity
Lipidation21N-myristoyl glycine Probable

Natural variations

Alternative sequence438 – 49154Missing in isoform 2.
VSP_013125
Natural variant5971G → A. Ref.6
Corresponds to variant rs12143301 [ dbSNP | Ensembl ].
VAR_051690
Natural variant6211Q → R. Ref.1 Ref.3 Ref.5
Corresponds to variant rs4656197 [ dbSNP | Ensembl ].
VAR_051691

Experimental info

Mutagenesis2 – 65GSENS → M: No Golgi targeting, accumulates in the cytoplasm. Ref.1
Sequence conflict6131M → T in AAN23123. Ref.1
Sequence conflict6131M → T in AAN41656. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 2F7D03681A4F641B

FASTA74282,857
        10         20         30         40         50         60 
MGSENSALKS YTLREPPFTL PSGLAVYPAV LQDGKFASVF VYKRENEDKV NKAAKHLKTL 

        70         80         90        100        110        120 
RHPCLLRFLS CTVEADGIHL VTERVQPLEV ALETLSSAEV CAGIYDILLA LIFLHDRGHL 

       130        140        150        160        170        180 
THNNVCLSSV FVSEDGHWKL GGMETVCKVS QATPEFLRSI QSIRDPASIP PEEMSPEFTT 

       190        200        210        220        230        240 
LPECHGHARD AFSFGTLVES LLTILNEQVS ADVLSSFQQT LHSTLLNPIP KCRPALCTLL 

       250        260        270        280        290        300 
SHDFFRNDFL EVVNFLKSLT LKSEEEKTEF FKFLLDRVSC LSEELIASRL VPLLLNQLVF 

       310        320        330        340        350        360 
AEPVAVKSFL PYLLGPKKDH AQGETPCLLS PALFQSRVIP VLLQLFEVHE EHVRMVLLSH 

       370        380        390        400        410        420 
IEAYVEHFTQ EQLKKVILPQ VLLGLRDTSD SIVAITLHSL AVLVSLLGPE VVVGGERTKI 

       430        440        450        460        470        480 
FKRTAPSFTK NTDLSLEDSP MCVVCSHHSQ ISPILENPFS SIFPKCFFSG STPINSKKHI 

       490        500        510        520        530        540 
QRDYYNTLLQ TGDPFSQPIK FPINGLSDVK NTSEDSENFP SSSKKSEEWP DWSEPEEPEN 

       550        560        570        580        590        600 
QTVNIQIWPR EPCDDVKSQC TTLDVEESSW DDCEPSSLDT KVNPGGGITA TKPVTSGEQK 

       610        620        630        640        650        660 
PIPALLSLTE ESMPWKSSLP QKISLVQRGD DADQIEPPKV SSQERPLKVP SELGLGEEFT 

       670        680        690        700        710        720 
IQVKKKPVKD PEMDWFADMI PEIKPSAAFL ILPELRTEMV PKKDDVSPVM QFSSKFAAAE 

       730        740 
ITEGEAEGWE EEGELNWEDN NW 

« Hide

Isoform 2 [UniParc].

Checksum: 96DCB00F38A7F928
Show »

FASTA68876,731

References

« Hide 'large scale' references
[1]"PACE-1, a novel protein that interacts with the C-terminal domain of ezrin."
Sullivan A., Uff C.R., Isacke C.M., Thorne R.F.
Exp. Cell Res. 284:224-238(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, INTERACTION WITH EZR, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, PHOSPHORYLATION, MYRISTOYLATION AT GLY-2, MUTAGENESIS OF 2-GLU--SER-6, VARIANT ARG-621.
Tissue: Kidney.
[2]Rhodes S., Huckle E.
Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ARG-621.
Tissue: Testis.
[4]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ARG-621.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT ALA-597.
Tissue: B-cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF540957 mRNA. Translation: AAN23123.1.
AY144493 mRNA. Translation: AAN41656.1.
AL117233 mRNA. Translation: CAB55300.1.
AK292507 mRNA. Translation: BAF85196.1.
AL031297 Genomic DNA. Translation: CAB52564.2.
AL031297 Genomic DNA. Translation: CAI23517.1.
AL031297 Genomic DNA. Translation: CAI23518.1.
CH471067 Genomic DNA. Translation: EAW90867.1.
BC014662 mRNA. Translation: AAH14662.1.
IPIIPI00217989.
IPI00237463.
RefSeqNP_065156.5. NM_020423.6.
NP_851607.2. NM_181093.3.
UniGeneHs.435560.
Hs.443551.

3D structure databases

ProteinModelPortalQ8IZE3.
ModBaseSearch...

Protein-protein interaction databases

IntActQ8IZE3. 3 interactions.
STRING9606.ENSP00000356744.

PTM databases

PhosphoSiteQ8IZE3.

Polymorphism databases

DMDM61214481.

Proteomic databases

PaxDbQ8IZE3.
PRIDEQ8IZE3.

Protocols and materials databases

DNASU57147.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000367770; ENSP00000356744; ENSG00000000457.
ENST00000367771; ENSP00000356745; ENSG00000000457.
ENST00000367772; ENSP00000356746; ENSG00000000457.
ENST00000423670; ENSP00000407993; ENSG00000000457.
GeneID57147.
KEGGhsa:57147.
UCSCuc001ggs.2. human.
uc001ggt.2. human.

Organism-specific databases

CTD57147.
GeneCardsGC01M169821.
HGNCHGNC:19285. SCYL3.
HPAHPA005624.
MIM608192. gene.
neXtProtNX_Q8IZE3.
PharmGKBPA142670945.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG293900.
HOVERGENHBG053485.
InParanoidQ8IZE3.
OMAWPDWSEP.
PhylomeDBQ8IZE3.

Gene expression databases

ArrayExpressQ8IZE3.
BgeeQ8IZE3.
CleanExHS_SCYL3.
GenevestigatorQ8IZE3.
GermOnlineENSG00000000457. Homo sapiens.

Family and domain databases

Gene3D1.25.10.10. 1 hit.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF48371. ARM-type_fold. 1 hit.
SSF56112. Kinase_like. 1 hit.
PROSITEPS50077. HEAT_REPEAT. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi57147.
NextBio63101.
SOURCESearch...

Entry information

Entry namePACE1_HUMAN
AccessionPrimary (citable) accession number: Q8IZE3
Secondary accession number(s): A8K8Z2 expand/collapse secondary AC list , Q5THA6, Q5THA8, Q8IZN9, Q96C56, Q9UBK6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: January 23, 2007
Last modified: April 3, 2013
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families