Reviewed, UniProtKB/Swiss-Prot Q8IZE3 (PACE1_HUMAN)

Names and origin

Protein names

Recommended name:
    Protein-associating with the carboxyl-terminal domain of ezrin
Alternative name(s):
    Ezrin-binding protein PACE-1
    SCY1-like protein 3

Gene names

Name:	SCYL3
Synonyms:	PACE1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	742 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in regulating cell adhesion/migration complexes in migrating cells. Ref.1
Subunit structure	Interacts with EZR/VIL2 C-terminal domain. Ref.1
Subcellular location	Cytoplasm. Golgi apparatus. Cell projection › lamellipodium. Note: Colocalized with EZR/VIL2, actin and CD44 in lamellipodia. Ref.1
Tissue specificity	Ubiquitously expressed. Ref.1
Domain	The protein kinase domain is predicted to be catalytically inactive.
Post-translational modification	May be myristoylated; myristoylation may target it to Golgi compartment. Phosphorylated. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. Contains 4 HEAT repeats. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cell projection Cytoplasm Golgi apparatus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
PTM	Lipoprotein Myristate Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell migration Ref.1 Non-traceable author statement. Source: UniProtKB protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Ref.1 Inferred from direct assay. Source: UniProtKB lamellipodium Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro protein binding Ref.1 Inferred from physical interaction. Source: UniProtKB protein kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EZR	P15311	2	EBI-1380680,EBI-1056902

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8IZE3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8IZE3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     438-491: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Probable
	Chain	2 – 742	741	Protein-associating with the carboxyl-terminal domain of ezrin		PRO_0000058167
Regions
	Domain	2 – 245	244	Protein kinase
	Repeat	199 – 238	40	HEAT 1
	Repeat	285 – 323	39	HEAT 2
	Repeat	333 – 370	38	HEAT 3
	Repeat	372 – 409	38	HEAT 4
	Region	548 – 742	195	Interaction with EZR
Amino acid modifications
	Modified residue	476	1	Phosphoserine By similarity
	Lipidation	2	1	N-myristoyl glycine Probable
Natural variations
	Alternative sequence	438 – 491	54	Missing in isoform 2.		VSP_013125
	Natural variant	597	1	G → A: dbSNP rs12143301. Ref.6		VAR_051690
	Natural variant	621	1	Q → R: dbSNP rs4656197. Ref.1 Ref.3 Ref.5		VAR_051691
Experimental info
	Mutagenesis	2 – 6	5	GSENS → M: No Golgi targeting, accumulates in the cytoplasm. Ref.1
	Sequence conflict	613	1	M → T in AAN23123. Ref.1
	Sequence conflict	613	1	M → T in AAN41656. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 2F7D03681A4F641B	FASTA	742	82,857
	10 20 30 40 50 60 MGSENSALKS YTLREPPFTL PSGLAVYPAV LQDGKFASVF VYKRENEDKV NKAAKHLKTL 70 80 90 100 110 120 RHPCLLRFLS CTVEADGIHL VTERVQPLEV ALETLSSAEV CAGIYDILLA LIFLHDRGHL 130 140 150 160 170 180 THNNVCLSSV FVSEDGHWKL GGMETVCKVS QATPEFLRSI QSIRDPASIP PEEMSPEFTT 190 200 210 220 230 240 LPECHGHARD AFSFGTLVES LLTILNEQVS ADVLSSFQQT LHSTLLNPIP KCRPALCTLL 250 260 270 280 290 300 SHDFFRNDFL EVVNFLKSLT LKSEEEKTEF FKFLLDRVSC LSEELIASRL VPLLLNQLVF 310 320 330 340 350 360 AEPVAVKSFL PYLLGPKKDH AQGETPCLLS PALFQSRVIP VLLQLFEVHE EHVRMVLLSH 370 380 390 400 410 420 IEAYVEHFTQ EQLKKVILPQ VLLGLRDTSD SIVAITLHSL AVLVSLLGPE VVVGGERTKI 430 440 450 460 470 480 FKRTAPSFTK NTDLSLEDSP MCVVCSHHSQ ISPILENPFS SIFPKCFFSG STPINSKKHI 490 500 510 520 530 540 QRDYYNTLLQ TGDPFSQPIK FPINGLSDVK NTSEDSENFP SSSKKSEEWP DWSEPEEPEN 550 560 570 580 590 600 QTVNIQIWPR EPCDDVKSQC TTLDVEESSW DDCEPSSLDT KVNPGGGITA TKPVTSGEQK 610 620 630 640 650 660 PIPALLSLTE ESMPWKSSLP QKISLVQRGD DADQIEPPKV SSQERPLKVP SELGLGEEFT 670 680 690 700 710 720 IQVKKKPVKD PEMDWFADMI PEIKPSAAFL ILPELRTEMV PKKDDVSPVM QFSSKFAAAE 730 740 ITEGEAEGWE EEGELNWEDN NW
	Isoform 2. Checksum: 96DCB00F38A7F928	FASTA	688	76,731
	10 20 30 40 50 60 MGSENSALKS YTLREPPFTL PSGLAVYPAV LQDGKFASVF VYKRENEDKV NKAAKHLKTL 70 80 90 100 110 120 RHPCLLRFLS CTVEADGIHL VTERVQPLEV ALETLSSAEV CAGIYDILLA LIFLHDRGHL 130 140 150 160 170 180 THNNVCLSSV FVSEDGHWKL GGMETVCKVS QATPEFLRSI QSIRDPASIP PEEMSPEFTT 190 200 210 220 230 240 LPECHGHARD AFSFGTLVES LLTILNEQVS ADVLSSFQQT LHSTLLNPIP KCRPALCTLL 250 260 270 280 290 300 SHDFFRNDFL EVVNFLKSLT LKSEEEKTEF FKFLLDRVSC LSEELIASRL VPLLLNQLVF 310 320 330 340 350 360 AEPVAVKSFL PYLLGPKKDH AQGETPCLLS PALFQSRVIP VLLQLFEVHE EHVRMVLLSH 370 380 390 400 410 420 IEAYVEHFTQ EQLKKVILPQ VLLGLRDTSD SIVAITLHSL AVLVSLLGPE VVVGGERTKI 430 440 450 460 470 480 FKRTAPSFTK NTDLSLEGDP FSQPIKFPIN GLSDVKNTSE DSENFPSSSK KSEEWPDWSE 490 500 510 520 530 540 PEEPENQTVN IQIWPREPCD DVKSQCTTLD VEESSWDDCE PSSLDTKVNP GGGITATKPV 550 560 570 580 590 600 TSGEQKPIPA LLSLTEESMP WKSSLPQKIS LVQRGDDADQ IEPPKVSSQE RPLKVPSELG 610 620 630 640 650 660 LGEEFTIQVK KKPVKDPEMD WFADMIPEIK PSAAFLILPE LRTEMVPKKD DVSPVMQFSS 670 680 KFAAAEITEG EAEGWEEEGE LNWEDNNW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PACE-1, a novel protein that interacts with the C-terminal domain of ezrin." Sullivan A., Uff C.R., Isacke C.M., Thorne R.F. Exp. Cell Res. 284:224-238(2003) [PubMed: 12651155] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, INTERACTION WITH EZR, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, PHOSPHORYLATION, MYRISTOYLATION AT GLY-2, MUTAGENESIS OF 2-GLU--SER-6, VARIANT ARG-621. Tissue: Kidney.
[2]	Rhodes S., Huckle E. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ARG-621. Tissue: Testis.
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ARG-621.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT ALA-597. Tissue: B-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF540957 mRNA. Translation: AAN23123.1. AY144493 mRNA. Translation: AAN41656.1. AL117233 mRNA. Translation: CAB55300.1. AK292507 mRNA. Translation: BAF85196.1. AL031297 Genomic DNA. Translation: CAB52564.2. AL031297 Genomic DNA. Translation: CAI23517.1. AL031297 Genomic DNA. Translation: CAI23518.1. CH471067 Genomic DNA. Translation: EAW90867.1. BC014662 mRNA. Translation: AAH14662.1.
IPI	IPI00217989. IPI00237463.
RefSeq	NP_065156.5. NP_851607.2.
UniGene	Hs.435560
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8IZE3. 3 interactions.
STRING	Q8IZE3.
PTM databases
PhosphoSite	Q8IZE3.
Proteomic databases
PRIDE	Q8IZE3.
Genome annotation databases
Ensembl	ENST00000367770; ENSP00000356744; ENSG00000000457; Homo sapiens. [Genome view] ENST00000367771; ENSP00000356745; ENSG00000000457; Homo sapiens. [Genome view] ENST00000367772; ENSP00000356746; ENSG00000000457; Homo sapiens. [Genome view] ENST00000423670; ENSP00000407993; ENSG00000000457; Homo sapiens. [Genome view]
GeneID	57147.
KEGG	hsa:57147.
NMPDR	fig|9606.3.peg.2600.
UCSC	uc001ggs.2. human. uc001ggt.2. human.
Organism-specific databases
CTD	57147.
GeneCards	GC01M168088.
H-InvDB	HIX0001320.
HGNC	HGNC:19285. SCYL3.
HPA	HPA005624.
MIM	608192. gene.
PharmGKB	PA142670945.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q8IZE3.
HOVERGEN	Q8IZE3.
OMA	KKKPVQD.
Gene expression databases
ArrayExpress	Q8IZE3.
Bgee	Q8IZE3.
CleanEx	HS_SCYL3.
Genevestigator	Q8IZE3.
GermOnline	ENSG00000000457. Homo sapiens.
Family and domain databases
InterPro	IPR000357. HEAT. IPR000719. Prot_kinase_core. [Graphical view]
Pfam	PF02985. HEAT. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS50077. HEAT_REPEAT. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	63101.
SOURCE	Search...

Entry information

Entry name

PACE1_HUMAN

Accession

Primary (citable) accession number: Q8IZE3
Secondary accession number(s): A8K8Z2 , Q5THA6, Q5THA8, Q8IZN9, Q96C56, Q9UBK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 69 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.