Reviewed,
UniProtKB/Swiss-Prot Q8IZE3 (PACE1_HUMAN)
Last modified
November 3, 2009.
Version 69.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Protein-associating with the carboxyl-terminal domain of ezrin Alternative name(s): Ezrin-binding protein PACE-1 SCY1-like protein 3 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 742 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | May play a role in regulating cell adhesion/migration complexes in migrating cells. Ref.1 |
| Subunit structure | Interacts with EZR/VIL2 C-terminal domain. Ref.1 |
| Subcellular location | Cytoplasm. Golgi apparatus. Cell projection › lamellipodium. Note: Colocalized with EZR/VIL2, actin and CD44 in lamellipodia. Ref.1 |
| Tissue specificity | Ubiquitously expressed. Ref.1 |
| Domain | The protein kinase domain is predicted to be catalytically inactive. |
| Post-translational modification | May be myristoylated; myristoylation may target it to Golgi compartment. Phosphorylated. Ref.1 |
| Sequence similarities | Belongs to the protein kinase superfamily. Contains 4 HEAT repeats. Contains 1 protein kinase domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell projection Cytoplasm Golgi apparatus |
| Coding sequence diversity | Alternative splicing Polymorphism |
| Domain | Repeat |
| PTM | Lipoprotein Myristate Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | cell migration Ref.1 Non-traceable author statement. Source: UniProtKB protein amino acid phosphorylationInferred from electronic annotation. Source: InterPro |
| Cellular component | Golgi apparatus Ref.1 Inferred from direct assay. Source: UniProtKB lamellipodium Ref.1Inferred from direct assay. Source: UniProtKB |
| Molecular function | ATP binding Inferred from electronic annotation. Source: InterPro protein binding Ref.1Inferred from physical interaction. Source: UniProtKB protein kinase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q8IZE3-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q8IZE3-2) The sequence of this isoform differs from the canonical sequence as follows: 438-491: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Probable | ||||||
| Chain | 2 – 742 | 741 | Protein-associating with the carboxyl-terminal domain of ezrin | PRO_0000058167 | |||||
Regions | |||||||||
| Domain | 2 – 245 | 244 | Protein kinase | ||||||
| Repeat | 199 – 238 | 40 | HEAT 1 | ||||||
| Repeat | 285 – 323 | 39 | HEAT 2 | ||||||
| Repeat | 333 – 370 | 38 | HEAT 3 | ||||||
| Repeat | 372 – 409 | 38 | HEAT 4 | ||||||
| Region | 548 – 742 | 195 | Interaction with EZR | ||||||
Amino acid modifications | |||||||||
| Modified residue | 476 | 1 | Phosphoserine By similarity | ||||||
| Lipidation | 2 | 1 | N-myristoyl glycine Probable | ||||||
Natural variations | |||||||||
| Alternative sequence | 438 – 491 | 54 | Missing in isoform 2. | VSP_013125 | |||||
| Natural variant | 597 | 1 | G → A: dbSNP rs12143301. Ref.6 | VAR_051690 | |||||
| Natural variant | 621 | 1 | Q → R: dbSNP rs4656197. Ref.1 Ref.3 Ref.5 | VAR_051691 | |||||
Experimental info | |||||||||
| Mutagenesis | 2 – 6 | 5 | GSENS → M: No Golgi targeting, accumulates in the cytoplasm. Ref.1 | ||||||
| Sequence conflict | 613 | 1 | M → T in AAN23123. Ref.1 | ||||||
| Sequence conflict | 613 | 1 | M → T in AAN41656. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "PACE-1, a novel protein that interacts with the C-terminal domain of ezrin." Sullivan A., Uff C.R., Isacke C.M., Thorne R.F. Exp. Cell Res. 284:224-238(2003) [PubMed: 12651155] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, INTERACTION WITH EZR, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, PHOSPHORYLATION, MYRISTOYLATION AT GLY-2, MUTAGENESIS OF 2-GLU--SER-6, VARIANT ARG-621. Tissue: Kidney. |
| [2] | Rhodes S., Huckle E. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). |
| [3] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ARG-621. Tissue: Testis. |
| [4] | "The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. Bentley D.R.Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [5] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ARG-621. |
| [6] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT ALA-597. Tissue: B-cell. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AF540957 mRNA. Translation: AAN23123.1. AY144493 mRNA. Translation: AAN41656.1. AL117233 mRNA. Translation: CAB55300.1. AK292507 mRNA. Translation: BAF85196.1. AL031297 Genomic DNA. Translation: CAB52564.2. AL031297 Genomic DNA. Translation: CAI23517.1. AL031297 Genomic DNA. Translation: CAI23518.1. CH471067 Genomic DNA. Translation: EAW90867.1. BC014662 mRNA. Translation: AAH14662.1. | |
| IPI | IPI00217989. IPI00237463. |
| RefSeq | NP_065156.5. NP_851607.2. |
| UniGene | Hs.435560 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q8IZE3. 3 interactions. |
| STRING | Q8IZE3. |
PTM databases | |
| PhosphoSite | Q8IZE3. |
Proteomic databases | |
| PRIDE | Q8IZE3. |
Genome annotation databases | |
| Ensembl | ENST00000367770; ENSP00000356744; ENSG00000000457; Homo sapiens. [Genome view] ENST00000367771; ENSP00000356745; ENSG00000000457; Homo sapiens. [Genome view] ENST00000367772; ENSP00000356746; ENSG00000000457; Homo sapiens. [Genome view] ENST00000423670; ENSP00000407993; ENSG00000000457; Homo sapiens. [Genome view] |
| GeneID | 57147. |
| KEGG | hsa:57147. |
| NMPDR | fig|9606.3.peg.2600. |
| UCSC | uc001ggs.2. human. uc001ggt.2. human. |
Organism-specific databases | |
| CTD | 57147. |
| GeneCards | GC01M168088. |
| H-InvDB | HIX0001320. |
| HGNC | HGNC:19285. SCYL3. |
| HPA | HPA005624. |
| MIM | 608192. gene. |
| PharmGKB | PA142670945. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | Q8IZE3. |
| HOVERGEN | Q8IZE3. |
| OMA | KKKPVQD. |
Gene expression databases | |
| ArrayExpress | Q8IZE3. |
| Bgee | Q8IZE3. |
| CleanEx | HS_SCYL3. |
| Genevestigator | Q8IZE3. |
| GermOnline | ENSG00000000457. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR000357. HEAT. IPR000719. Prot_kinase_core. [Graphical view] |
| Pfam | PF02985. HEAT. 1 hit. [Graphical view] |
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| PROSITE | PS50077. HEAT_REPEAT. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 63101. |
| SOURCE | Search... |
Entry information
| Entry name | PACE1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q8IZE3 Secondary accession number(s): A8K8Z2 Q9UBK6 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 1 Human chromosome 1: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| SIMILARITY comments Index of protein domains and families |

Clusters with


