ID   RUSD2_HUMAN             Reviewed;         545 AA.
AC   Q8IZ73; Q7L989; Q92939; Q96IA7; Q96N50;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2007, sequence version 2.
DT   25-JAN-2012, entry version 69.
DE   RecName: Full=RNA pseudouridylate synthase domain-containing protein 2;
GN   Name=RPUSD2; Synonyms=C15orf19;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Lymph;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 334-545.
RC   TISSUE=Lung;
RA   Sampson M., McClendon D., Wiley K., Barlow C.;
RL   Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Cervix carcinoma;
RX   PubMed=17924679; DOI=10.1021/pr070152u;
RA   Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D.;
RT   "Improved titanium dioxide enrichment of phosphopeptides from HeLa
RT   cells and high confident phosphopeptide identification by cross-
RT   validation of MS/MS and MS/MS/MS spectra.";
RL   J. Proteome Res. 6:4150-4162(2007).
RN   [5]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Cervix carcinoma;
RX   PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA   Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA   Elledge S.J., Gygi S.P.;
RT   "A quantitative atlas of mitotic phosphorylation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA   Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase rluA family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB07777.1; Type=Frameshift; Positions=494, 497;
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DR   EMBL; AK055971; BAB71059.1; -; mRNA.
DR   EMBL; BC007697; AAH07697.1; -; mRNA.
DR   EMBL; BC016967; AAH16967.2; -; mRNA.
DR   EMBL; U67934; AAB07777.1; ALT_FRAME; Genomic_DNA.
DR   IPI; IPI00150963; -.
DR   RefSeq; NP_689473.1; NM_152260.1.
DR   UniGene; Hs.173311; -.
DR   HSSP; P0AA39; 1XPI.
DR   ProteinModelPortal; Q8IZ73; -.
DR   SMR; Q8IZ73; 152-391.
DR   IntAct; Q8IZ73; 1.
DR   STRING; Q8IZ73; -.
DR   PhosphoSite; Q8IZ73; -.
DR   DMDM; 158563848; -.
DR   PeptideAtlas; Q8IZ73; -.
DR   PRIDE; Q8IZ73; -.
DR   Ensembl; ENST00000315616; ENSP00000323288; ENSG00000166133.
DR   GeneID; 27079; -.
DR   KEGG; hsa:27079; -.
DR   UCSC; uc001zmd.1; human.
DR   CTD; 27079; -.
DR   GeneCards; GC15P040861; -.
DR   H-InvDB; HIX0012134; -.
DR   HGNC; HGNC:24180; RPUSD2.
DR   HPA; HPA042079; -.
DR   HPA; HPA042194; -.
DR   neXtProt; NX_Q8IZ73; -.
DR   PharmGKB; PA134866412; -.
DR   eggNOG; prNOG10606; -.
DR   GeneTree; ENSGT00420000029802; -.
DR   HOGENOM; HBG395845; -.
DR   HOVERGEN; HBG103625; -.
DR   InParanoid; Q8IZ73; -.
DR   OMA; YKGPDFE; -.
DR   OrthoDB; EOG4DV5KW; -.
DR   PhylomeDB; Q8IZ73; -.
DR   NextBio; 49685; -.
DR   ArrayExpress; Q8IZ73; -.
DR   Bgee; Q8IZ73; -.
DR   CleanEx; HS_RPUSD2; -.
DR   Genevestigator; Q8IZ73; -.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom.
DR   InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluD.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; PsdUridine_synth_cat_dom; 1.
DR   TIGRFAMs; TIGR00005; RluA_subfam; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Phosphoprotein; Reference proteome.
FT   CHAIN         1    545       RNA pseudouridylate synthase domain-
FT                                containing protein 2.
FT                                /FTId=PRO_0000300819.
FT   COMPBIAS    458    461       Poly-Ala.
FT   ACT_SITE    274    274       By similarity.
FT   MOD_RES      68     68       Phosphoserine.
FT   CONFLICT    336    336       R -> L (in Ref. 3; AAB07777).
FT   CONFLICT    492    492       N -> H (in Ref. 3; AAB07777).
FT   CONFLICT    499    499       C -> S (in Ref. 3; AAB07777).
SQ   SEQUENCE   545 AA;  61311 MW;  16E8B71918A6E0B7 CRC64;
     MWLDRRGWLR VLGHWRYDLR RPSFTRTWSG DKGPMAETVS TQVGTEGGLR ASHQQNGDAG
     GDAKVELSPG PPKPAGREVE PAPVGGEHPS AAAPGPGKHK KRRGATRERV VPPPKKRRTG
     VSFGDEHFAE TSYYFEGGLR KVRPYYFDFR TYCKGRWVGH SLLHVFSTEF RAQPLAYYEA
     AVRAGRLQLN EKPVQDLNIV LKDNDFLRNT VHRHEPPVTA EPIRLLAENE DVVVVDKPSS
     IPVHPCGRFR HNTVIFILGK EHQLKELHPL HRLDRLTSGV LMFAKTAAVS ERIHEQVRDR
     QLEKEYVCRV EGEFPTEEVT CKEPILVVSY KVGVCRVDPR GKPCETVFQR LSYNGQSSVV
     RCRPLTGRTH QIRVHLQFLG HPILNDPIYN SVAWGPSRGR GGYIPKTNEE LLRDLVAEHQ
     AKQSLDVLDL CEGDLSPGLT DSTAPSSELG KDDLEELAAA AQKMEEVAEA APQELDTIAL
     ASEKAVETDV MNQETDPLCA ECRLVRQDPL PQDLVMFLHA LRYKGPGFEY FSPMPAWAQD
     DWQKD
//