Reviewed, UniProtKB/Swiss-Prot Q8IZ73 (RUSD2_HUMAN)

Names and origin

Protein names

Recommended name:
    RNA pseudouridylate synthase domain-containing protein 2

Gene names

Name:	RPUSD2
Synonyms:	C15orf19

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	545 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Sequence similarities	Belongs to the pseudouridine synthase rluA family.
Sequence caution	The sequence AAB07777.1 differs from that shown. Reason: Frameshift at positions 494 and 497.

Ontologies

Keywords
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pseudouridine synthesis Inferred from electronic annotation. Source: InterPro
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct pseudouridine synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GRB2	P62993	1	EBI-1965305,EBI-401755

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 545	545	RNA pseudouridylate synthase domain-containing protein 2		PRO_0000300819
Regions
	Compositional bias	458 – 461	4	Poly-Ala
Sites
	Active site	274	1	By similarity
Amino acid modifications
	Modified residue	68	1	Phosphoserine Ref.4 Ref.5
Experimental info
	Sequence conflict	336	1	R → L in AAB07777. Ref.3
	Sequence conflict	492	1	N → H in AAB07777. Ref.3
	Sequence conflict	499	1	C → S in AAB07777. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8IZ73-1 [UniParc]. Last modified April 3, 2007. Version 2. Checksum: 16E8B71918A6E0B7	FASTA	545	61,311
	10 20 30 40 50 60 MWLDRRGWLR VLGHWRYDLR RPSFTRTWSG DKGPMAETVS TQVGTEGGLR ASHQQNGDAG 70 80 90 100 110 120 GDAKVELSPG PPKPAGREVE PAPVGGEHPS AAAPGPGKHK KRRGATRERV VPPPKKRRTG 130 140 150 160 170 180 VSFGDEHFAE TSYYFEGGLR KVRPYYFDFR TYCKGRWVGH SLLHVFSTEF RAQPLAYYEA 190 200 210 220 230 240 AVRAGRLQLN EKPVQDLNIV LKDNDFLRNT VHRHEPPVTA EPIRLLAENE DVVVVDKPSS 250 260 270 280 290 300 IPVHPCGRFR HNTVIFILGK EHQLKELHPL HRLDRLTSGV LMFAKTAAVS ERIHEQVRDR 310 320 330 340 350 360 QLEKEYVCRV EGEFPTEEVT CKEPILVVSY KVGVCRVDPR GKPCETVFQR LSYNGQSSVV 370 380 390 400 410 420 RCRPLTGRTH QIRVHLQFLG HPILNDPIYN SVAWGPSRGR GGYIPKTNEE LLRDLVAEHQ 430 440 450 460 470 480 AKQSLDVLDL CEGDLSPGLT DSTAPSSELG KDDLEELAAA AQKMEEVAEA APQELDTIAL 490 500 510 520 530 540 ASEKAVETDV MNQETDPLCA ECRLVRQDPL PQDLVMFLHA LRYKGPGFEY FSPMPAWAQD DWQKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph.
[3]	Sampson M., McClendon D., Wiley K., Barlow C. Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 334-545. Tissue: Lung.
[4]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68, MASS SPECTROMETRY. Tissue: Epithelium.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68, MASS SPECTROMETRY.
[6]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK055971 mRNA. Translation: BAB71059.1. BC007697 mRNA. Translation: AAH07697.1. BC016967 mRNA. Translation: AAH16967.2. U67934 Genomic DNA. Translation: AAB07777.1. Frameshift.
IPI	IPI00150963.
RefSeq	NP_689473.1.
UniGene	Hs.173311
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8IZ73. 1 interaction.
STRING	Q8IZ73.
PTM databases
PhosphoSite	Q8IZ73.
Proteomic databases
PeptideAtlas	Q8IZ73.
PRIDE	Q8IZ73.
Genome annotation databases
Ensembl	ENST00000315616; ENSP00000323288; ENSG00000166133; Homo sapiens. [Genome view] ENST00000417769; ENSP00000388146; ENSG00000166133; Homo sapiens. [Genome view]
GeneID	27079.
KEGG	hsa:27079.
UCSC	uc001zmd.1. human.
Organism-specific databases
CTD	27079.
GeneCards	GC15P038649.
HGNC	HGNC:24180. RPUSD2.
PharmGKB	PA134866412.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q8IZ73.
OMA	HALRYKG.
Gene expression databases
ArrayExpress	Q8IZ73.
Bgee	Q8IZ73.
CleanEx	HS_RPUSD2.
Genevestigator	Q8IZ73.
Family and domain databases
InterPro	IPR006225. PsdUridine_synth_RluC/D. IPR006224. PsdUridine_synth_RluC/D_CS. IPR006145. PsdUridine_synth_RsuA/RluD. [Graphical view]
Pfam	PF00849. PseudoU_synth_2. 1 hit. [Graphical view]
ProDom	PD001819. PseudoU_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00005. rluA_subfam. 1 hit.
PROSITE	PS01129. PSI_RLU. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	49685.

Entry information

Entry name

RUSD2_HUMAN

Accession

Primary (citable) accession number: Q8IZ73
Secondary accession number(s): Q7L989 , Q92939, Q96IA7, Q96N50

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	April 3, 2007
Last modified:	November 3, 2009
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.