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Protein

tRNA (uracil-5-)-methyltransferase homolog A

Gene

TRMT2A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in nucleic acid metabolism and/or modifications.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei411S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei461S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei510S-adenosyl-L-methioninePROSITE-ProRule annotation1
Active sitei538NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB
  • RNA methyltransferase activity Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (uracil-5-)-methyltransferase homolog A (EC:2.1.1.-)
Alternative name(s):
HpaII tiny fragments locus 9c protein
Gene namesi
Name:TRMT2A
Synonyms:HTF9C
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:24974. TRMT2A.

Pathology & Biotechi

Organism-specific databases

DisGeNETi27037.
OpenTargetsiENSG00000099899.
PharmGKBiPA164726751.

Polymorphism and mutation databases

BioMutaiTRMT2A.
DMDMi51316479.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000816141 – 625tRNA (uracil-5-)-methyltransferase homolog AAdd BLAST625

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei378PhosphoserineCombined sources1
Modified residuei602PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8IZ69.
MaxQBiQ8IZ69.
PaxDbiQ8IZ69.
PeptideAtlasiQ8IZ69.
PRIDEiQ8IZ69.

PTM databases

iPTMnetiQ8IZ69.
PhosphoSitePlusiQ8IZ69.

Expressioni

Gene expression databases

BgeeiENSG00000099899.
CleanExiHS_TRMT2A.
ExpressionAtlasiQ8IZ69. baseline and differential.
GenevisibleiQ8IZ69. HS.

Organism-specific databases

HPAiHPA001077.

Interactioni

Protein-protein interaction databases

BioGridi117968. 56 interactors.
IntActiQ8IZ69. 25 interactors.
MINTiMINT-2809942.
STRINGi9606.ENSP00000252136.

Structurei

3D structure databases

ProteinModelPortaliQ8IZ69.
SMRiQ8IZ69.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini73 – 146RRMPROSITE-ProRule annotationAdd BLAST74

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili180 – 209Sequence analysisAdd BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi153 – 156Poly-Arg4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.PROSITE-ProRule annotation
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2187. Eukaryota.
COG2265. LUCA.
GeneTreeiENSGT00530000063723.
HOGENOMiHOG000232103.
HOVERGENiHBG052043.
InParanoidiQ8IZ69.
KOiK15332.
PhylomeDBiQ8IZ69.
TreeFamiTF314569.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR029063. SAM-dependent_MTases.
IPR010280. U5_MeTrfase_fam.
[Graphical view]
PfamiPF05958. tRNA_U5-meth_tr. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
SSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
PS51687. SAM_MT_RNA_M5U. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8IZ69-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSENLDNEGP KPMESCGQES SSALSCPTVS VPPAAPAALE EVEKEGAGAA
60 70 80 90 100
TGPGPQPGLY SYIRDDLFTS EIFKLELQNV PRHASFSDVR RFLGRFGLQP
110 120 130 140 150
HKTKLFGQPP CAFVTFRSAA ERDKALRVLH GALWKGRPLS VRLARPKADP
160 170 180 190 200
MARRRRQEGE SEPPVTRVAD VVTPLWTVPY AEQLERKQLE CEQVLQKLAK
210 220 230 240 250
EIGSTNRALL PWLLEQRHKH NKACCPLEGV RPSPQQTEYR NKCEFLVGVG
260 270 280 290 300
VDGEDNTVGC RLGKYKGGTC AVAAPFDTVH IPEATKQVVK AFQEFIRSTP
310 320 330 340 350
YSAYDPETYT GHWKQLTVRT SRRHQAMAIA YFHPQKLSPE ELAELKTSLA
360 370 380 390 400
QHFTAGPGRA SGVTCLYFVE EGQRKTPSQE GLPLEHVAGD RCIHEDLLGL
410 420 430 440 450
TFRISPHAFF QVNTPAAEVL YTVIQDWAQL DAGSMVLDVC CGTGTIGLAL
460 470 480 490 500
ARKVKRVIGV ELCPEAVEDA RVNAQDNELS NVEFHCGRAE DLVPTLVSRL
510 520 530 540 550
ASQHLVAILD PPRAGLHSKV ILAIRRAKNL RRLLYVSCNP RAAMGNFVDL
560 570 580 590 600
CRAPSNRVKG IPFRPVKAVA VDLFPQTPHC EMLILFERVE HPNGTGVLGP
610 620
HSPPAQPTPG PPDNTLQETG TFPSS
Length:625
Mass (Da):68,726
Last modified:March 1, 2004 - v2
Checksum:i2D310E521D846E06
GO
Isoform 2 (identifier: Q8IZ69-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     550-562: LCRAPSNRVKGIP → APLFPPQPLQSPI
     563-625: Missing.

Note: No experimental confirmation available.
Show »
Length:562
Mass (Da):61,979
Checksum:iF0D87D27746DE1DB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti498S → G in BAB71349 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_033721602S → R.Corresponds to variant rs447017dbSNPEnsembl.1
Natural variantiVAR_035482604P → S in a breast cancer sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_011322550 – 562LCRAP…VKGIP → APLFPPQPLQSPI in isoform 2. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_011323563 – 625Missing in isoform 2. 1 PublicationAdd BLAST63

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR456354 mRNA. Translation: CAG30240.1.
AK025106 mRNA. Translation: BAB15067.1.
AK057029 mRNA. Translation: BAB71349.1.
AC006547 Genomic DNA. No translation available.
CH471176 Genomic DNA. Translation: EAX02996.1.
CH471176 Genomic DNA. Translation: EAX02997.1.
BC013352 mRNA. Translation: AAH13352.2.
BC017184 mRNA. Translation: AAH17184.2.
BC108251 mRNA. Translation: AAI08252.1.
CCDSiCCDS13774.1. [Q8IZ69-1]
CCDS58793.1. [Q8IZ69-2]
RefSeqiNP_001244923.1. NM_001257994.1. [Q8IZ69-2]
NP_073564.3. NM_022727.5. [Q8IZ69-1]
NP_892029.2. NM_182984.4. [Q8IZ69-1]
UniGeneiHs.713579.

Genome annotation databases

EnsembliENST00000252136; ENSP00000252136; ENSG00000099899. [Q8IZ69-1]
ENST00000403707; ENSP00000385807; ENSG00000099899. [Q8IZ69-1]
ENST00000404751; ENSP00000384968; ENSG00000099899. [Q8IZ69-2]
GeneIDi27037.
KEGGihsa:27037.
UCSCiuc002zrk.3. human. [Q8IZ69-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR456354 mRNA. Translation: CAG30240.1.
AK025106 mRNA. Translation: BAB15067.1.
AK057029 mRNA. Translation: BAB71349.1.
AC006547 Genomic DNA. No translation available.
CH471176 Genomic DNA. Translation: EAX02996.1.
CH471176 Genomic DNA. Translation: EAX02997.1.
BC013352 mRNA. Translation: AAH13352.2.
BC017184 mRNA. Translation: AAH17184.2.
BC108251 mRNA. Translation: AAI08252.1.
CCDSiCCDS13774.1. [Q8IZ69-1]
CCDS58793.1. [Q8IZ69-2]
RefSeqiNP_001244923.1. NM_001257994.1. [Q8IZ69-2]
NP_073564.3. NM_022727.5. [Q8IZ69-1]
NP_892029.2. NM_182984.4. [Q8IZ69-1]
UniGeneiHs.713579.

3D structure databases

ProteinModelPortaliQ8IZ69.
SMRiQ8IZ69.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117968. 56 interactors.
IntActiQ8IZ69. 25 interactors.
MINTiMINT-2809942.
STRINGi9606.ENSP00000252136.

PTM databases

iPTMnetiQ8IZ69.
PhosphoSitePlusiQ8IZ69.

Polymorphism and mutation databases

BioMutaiTRMT2A.
DMDMi51316479.

Proteomic databases

EPDiQ8IZ69.
MaxQBiQ8IZ69.
PaxDbiQ8IZ69.
PeptideAtlasiQ8IZ69.
PRIDEiQ8IZ69.

Protocols and materials databases

DNASUi27037.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000252136; ENSP00000252136; ENSG00000099899. [Q8IZ69-1]
ENST00000403707; ENSP00000385807; ENSG00000099899. [Q8IZ69-1]
ENST00000404751; ENSP00000384968; ENSG00000099899. [Q8IZ69-2]
GeneIDi27037.
KEGGihsa:27037.
UCSCiuc002zrk.3. human. [Q8IZ69-1]

Organism-specific databases

CTDi27037.
DisGeNETi27037.
GeneCardsiTRMT2A.
HGNCiHGNC:24974. TRMT2A.
HPAiHPA001077.
MIMi611151. gene.
neXtProtiNX_Q8IZ69.
OpenTargetsiENSG00000099899.
PharmGKBiPA164726751.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2187. Eukaryota.
COG2265. LUCA.
GeneTreeiENSGT00530000063723.
HOGENOMiHOG000232103.
HOVERGENiHBG052043.
InParanoidiQ8IZ69.
KOiK15332.
PhylomeDBiQ8IZ69.
TreeFamiTF314569.

Miscellaneous databases

ChiTaRSiTRMT2A. human.
GenomeRNAii27037.
PROiQ8IZ69.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000099899.
CleanExiHS_TRMT2A.
ExpressionAtlasiQ8IZ69. baseline and differential.
GenevisibleiQ8IZ69. HS.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR029063. SAM-dependent_MTases.
IPR010280. U5_MeTrfase_fam.
[Graphical view]
PfamiPF05958. tRNA_U5-meth_tr. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
SSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
PS51687. SAM_MT_RNA_M5U. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRM2A_HUMAN
AccessioniPrimary (citable) accession number: Q8IZ69
Secondary accession number(s): D3DX25
, Q32P57, Q96ME6, Q9H732
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: March 1, 2004
Last modified: November 30, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.