Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ras and EF-hand domain-containing protein

Gene

RASEF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds predominantly GDP, and also GTP.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi21 – 32121Add
BLAST
Calcium bindingi55 – 66122Add
BLAST
Nucleotide bindingi548 – 5558GTPCurated
Nucleotide bindingi596 – 6005GTPCurated
Nucleotide bindingi654 – 6574GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, GTP-binding, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras and EF-hand domain-containing protein
Alternative name(s):
Ras-related protein Rab-45
Gene namesi
Name:RASEF
Synonyms:RAB45
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:26464. RASEF.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi555 – 5551S → N: Impairs nucleotide binding and perinuclear localization. 1 Publication
Mutagenesisi600 – 6001Q → L: Favors GTP association. 1 Publication

Organism-specific databases

PharmGKBiPA134867201.

Polymorphism and mutation databases

DMDMi74750780.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 740740Ras and EF-hand domain-containing proteinPRO_0000299577Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei377 – 3771PhosphoserineBy similarity
Modified residuei383 – 3831PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8IZ41.
PaxDbiQ8IZ41.
PeptideAtlasiQ8IZ41.
PRIDEiQ8IZ41.
TopDownProteomicsiQ8IZ41-2. [Q8IZ41-2]

PTM databases

iPTMnetiQ8IZ41.
PhosphoSiteiQ8IZ41.

Expressioni

Tissue specificityi

Down-regulated in cutaneous melanoma cells but not in breast cancer cells.1 Publication

Gene expression databases

BgeeiENSG00000165105.
CleanExiHS_RASEF.
GenevisibleiQ8IZ41. HS.

Organism-specific databases

HPAiHPA021431.
HPA051606.

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

BioGridi127651. 2 interactions.
STRINGi9606.ENSP00000365630.

Structurei

Secondary structure

1
740
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 2017Combined sources
Beta strandi25 – 295Combined sources
Helixi30 – 3910Combined sources
Helixi44 – 5411Combined sources
Beta strandi59 – 624Combined sources
Helixi64 – 674Combined sources
Helixi69 – 735Combined sources
Beta strandi542 – 5498Combined sources
Helixi554 – 56310Combined sources
Beta strandi579 – 5857Combined sources
Beta strandi588 – 5969Combined sources
Helixi604 – 61310Combined sources
Beta strandi615 – 6228Combined sources
Helixi626 – 6305Combined sources
Helixi632 – 64211Combined sources
Beta strandi649 – 6546Combined sources
Helixi656 – 6583Combined sources
Helixi659 – 6646Combined sources
Helixi672 – 68211Combined sources
Beta strandi685 – 6884Combined sources
Turni691 – 6933Combined sources
Helixi697 – 70812Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2P5SX-ray2.15A/B533-712[»]
2PMYX-ray2.30A/B3-75[»]
ProteinModelPortaliQ8IZ41.
SMRiQ8IZ41. Positions 3-75, 513-711.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8IZ41.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 4235EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini42 – 7736EF-hand 2PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili175 – 356182Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated
Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0078. Eukaryota.
ENOG410XPUI. LUCA.
GeneTreeiENSGT00820000127021.
HOGENOMiHOG000008699.
HOVERGENiHBG057757.
InParanoidiQ8IZ41.
KOiK17199.
OMAiKHQRGFQ.
OrthoDBiEOG091G0CBJ.
PhylomeDBiQ8IZ41.
TreeFamiTF313106.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
PF00071. Ras. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS51419. RAB. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8IZ41-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEADGDGEEL ARLRSVFAAC DANRSGRLER EEFRALCTEL RVRPADAEAV
60 70 80 90 100
FQRLDADRDG AITFQEFARG FLGSLRGGRR RDWGPLDPAP AVSEAGPETH
110 120 130 140 150
DSEEDEGDED AAAALATSCG PASPGRAWQD FQARLGDEAK FIPREEQVST
160 170 180 190 200
LYQNINLVEP RLIQPYEHVI KNFIREIRLQ STEMENLAIA VKRAQDKAAM
210 220 230 240 250
QLSELEEEMD QRIQAAEHKT RKDEKRKAEE ALSDLRRQYE TEVGDLQVTI
260 270 280 290 300
KKLRKLEEQS KRVSQKEDVA ALKKQIYDLS MENQKVKKDL LEAQTNIAFL
310 320 330 340 350
QSELDALKSD YADQSLNTER DLEIIRAYTE DRNSLERQIE ILQTANRKLH
360 370 380 390 400
DSNDGLRSAL ENSYSKFNRS LHINNISPGN TISRSSPKFI GHSPQPLGYD
410 420 430 440 450
RSSRSSYVDE DCDSLALCDP LQRTNCEVDS LPESCFDSGL STLRDPNEYD
460 470 480 490 500
SEVEYKHQRG FQRSHGVQES FGGDASDTDV PDIRDEETFG LEDVASVLDW
510 520 530 540 550
KPQGSVSEGS IVSSSRKPIS ALSPQTDLVD DNAKSFSSQK AYKIVLAGDA
560 570 580 590 600
AVGKSSFLMR LCKNEFRENI SATLGVDFQM KTLIVDGERT VLQLWDTAGQ
610 620 630 640 650
ERFRSIAKSY FRKADGVLLL YDVTCEKSFL NIREWVDMIE DAAHETVPIM
660 670 680 690 700
LVGNKADIRD TAATEGQKCV PGHFGEKLAM TYGALFCETS AKDGSNIVEA
710 720 730 740
VLHLAREVKK RTDKDDSRSI TNLTGTNSKK SPQMKNCCNG
Length:740
Mass (Da):82,879
Last modified:March 1, 2003 - v1
Checksum:i89AFCF4C159760F0
GO
Isoform 2 (identifier: Q8IZ41-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     145-189: EEQVSTLYQN...QSTEMENLAI → FGYTISKPAF...IEEGGAKMAE
     190-740: Missing.

Note: No experimental confirmation available.
Show »
Length:189
Mass (Da):20,736
Checksum:i472825570664F33D
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti262 – 2621R → C.1 Publication
Corresponds to variant rs4146960 [ dbSNP | Ensembl ].
VAR_034858

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei145 – 18945EEQVS…ENLAI → FGYTISKPAFVFVRHLITLC ALSWAKKEGRETLWTIEEGG AKMAE in isoform 2. 1 PublicationVSP_027766Add
BLAST
Alternative sequencei190 – 740551Missing in isoform 2. 1 PublicationVSP_027767Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056176 mRNA. No translation available.
AL499602 Genomic DNA. Translation: CAH70678.1.
AL499602 Genomic DNA. Translation: CAH70679.1.
CH471089 Genomic DNA. Translation: EAW62643.1.
BC023566 mRNA. Translation: AAH23566.1.
CCDSiCCDS6662.1. [Q8IZ41-1]
RefSeqiNP_689786.2. NM_152573.3. [Q8IZ41-1]
UniGeneiHs.129136.
Hs.677846.

Genome annotation databases

EnsembliENST00000340717; ENSP00000345651; ENSG00000165105. [Q8IZ41-2]
ENST00000376447; ENSP00000365630; ENSG00000165105. [Q8IZ41-1]
GeneIDi158158.
KEGGihsa:158158.
UCSCiuc004amo.2. human. [Q8IZ41-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056176 mRNA. No translation available.
AL499602 Genomic DNA. Translation: CAH70678.1.
AL499602 Genomic DNA. Translation: CAH70679.1.
CH471089 Genomic DNA. Translation: EAW62643.1.
BC023566 mRNA. Translation: AAH23566.1.
CCDSiCCDS6662.1. [Q8IZ41-1]
RefSeqiNP_689786.2. NM_152573.3. [Q8IZ41-1]
UniGeneiHs.129136.
Hs.677846.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2P5SX-ray2.15A/B533-712[»]
2PMYX-ray2.30A/B3-75[»]
ProteinModelPortaliQ8IZ41.
SMRiQ8IZ41. Positions 3-75, 513-711.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127651. 2 interactions.
STRINGi9606.ENSP00000365630.

PTM databases

iPTMnetiQ8IZ41.
PhosphoSiteiQ8IZ41.

Polymorphism and mutation databases

DMDMi74750780.

Proteomic databases

MaxQBiQ8IZ41.
PaxDbiQ8IZ41.
PeptideAtlasiQ8IZ41.
PRIDEiQ8IZ41.
TopDownProteomicsiQ8IZ41-2. [Q8IZ41-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000340717; ENSP00000345651; ENSG00000165105. [Q8IZ41-2]
ENST00000376447; ENSP00000365630; ENSG00000165105. [Q8IZ41-1]
GeneIDi158158.
KEGGihsa:158158.
UCSCiuc004amo.2. human. [Q8IZ41-1]

Organism-specific databases

CTDi158158.
GeneCardsiRASEF.
HGNCiHGNC:26464. RASEF.
HPAiHPA021431.
HPA051606.
MIMi611344. gene.
neXtProtiNX_Q8IZ41.
PharmGKBiPA134867201.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0078. Eukaryota.
ENOG410XPUI. LUCA.
GeneTreeiENSGT00820000127021.
HOGENOMiHOG000008699.
HOVERGENiHBG057757.
InParanoidiQ8IZ41.
KOiK17199.
OMAiKHQRGFQ.
OrthoDBiEOG091G0CBJ.
PhylomeDBiQ8IZ41.
TreeFamiTF313106.

Miscellaneous databases

ChiTaRSiRASEF. human.
EvolutionaryTraceiQ8IZ41.
GenomeRNAii158158.
PROiQ8IZ41.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000165105.
CleanExiHS_RASEF.
GenevisibleiQ8IZ41. HS.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
PF00071. Ras. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRASEF_HUMAN
AccessioniPrimary (citable) accession number: Q8IZ41
Secondary accession number(s): A6NC29, Q96N04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.