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Protein

Ankyrin repeat domain-containing protein 13A

Gene

ANKRD13A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Does not bind 'Lys-48'-linked ubiquitin. Positively regulates the internalization of ligand-activated EGFR by binding to the Ub moiety of ubiquitinated EGFR at the cell membrane.1 Publication

Names & Taxonomyi

Protein namesi
Recommended name:
Ankyrin repeat domain-containing protein 13A
Alternative name(s):
Protein KE03
Gene namesi
Name:ANKRD13A
Synonyms:ANKRD13
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:21268. ANKRD13A.

Subcellular locationi

  • Cell membrane 1 Publication
  • Late endosome 1 Publication

  • Note: Interaction with EGFR may enhance association with the cell membrane.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi491 – 4955AIQQS → GIQQA: No effect on 'Lys-63'-linked ubiquitin binding and interaction with EGFR. No effect on 'Lys-63'-linked ubiquitin binding and interaction with EGFR; when associated with 527-G--A-531. Inhibits 'Lys-63'-linked ubiquitin binding; when associated with 527-G--A-531 and 557-G--A-561. Abolishes 'Lys-63'-linked ubiquitin binding and interaction with EGFR; when associated with 527-G--A-531, 557-G--A-561 and 582-G--A-586. 1 Publication
Mutagenesisi527 – 5315AIQES → GIQEA: No effect on 'Lys-63'-linked ubiquitin binding and interaction with EGFR. No effect on 'Lys-63'-linked ubiquitin binding and interaction with EGFR; when associated with 491-G--A-495. Inhibits 'Lys-63'-linked ubiquitin binding; when associated with 557-G--A-561. Inhibits 'Lys-63'-linked ubiquitin binding; when associated with 491-G--A-495 and 557-G--A-561. Strongly inhibits 'Lys-63'-linked ubiquitin binding; when associated with 557-G--A-561 and 582-G--A-586. Abolishes 'Lys-63'-linked ubiquitin binding and interaction with EGFR; when associated with 491-G--A-495, 557-G--A-561 and 582-G--A-586. 1 Publication
Mutagenesisi557 – 5615AMELS → GMELA: Slightly inhibits 'Lys-63'-linked ubiquitin binding and strongly inhibits interaction with EGFR. No effect on 'Lys-63'-linked ubiquitin binding and interaction with EGFR; when associated with 527-G--A-531. Inhibits 'Lys-63'-linked ubiquitin binding and interaction with EGFR; when associated with 491-G--A-495 and 527-G--A-531. Abolishes 'Lys-63'-linked ubiquitin binding and interaction with EGFR; when associated with 582-V--S-586. Abolishes 'Lys-63'-linked ubiquitin binding and interaction with EGFR; when associated with 491-G--A-495, 527-G--A-531 and 582-G--A-586. 1 Publication
Mutagenesisi582 – 5865VLQLS → GLQLA: Abolishes 'Lys-63'-linked ubiquitin binding and strongly inhibits interaction with EGFR. Abolishes 'Lys-63'-linked ubiquitin binding and interaction with EGFR; when associated with 557-G--A-561. Strongly inhibits 'Lys-63'-linked ubiquitin binding; when associated with 527-G--A-531 and 557-G--A-561. Abolishes 'Lys-63'-linked ubiquitin binding and interaction with EGFR; when associated with 491-G--A-495, 527-G--A-531 and 557-G--A-561. 1 Publication

Organism-specific databases

PharmGKBiPA134884324.

Polymorphism and mutation databases

BioMutaiANKRD13A.
DMDMi145559439.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 590590Ankyrin repeat domain-containing protein 13APRO_0000066909Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei205 – 2051PhosphoserineBy similarity
Modified residuei586 – 5861PhosphoserineCombined sources

Post-translational modificationi

Monoubiquitinated, inhibits interaction with ubiquitinated EGFR.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8IZ07.
MaxQBiQ8IZ07.
PaxDbiQ8IZ07.
PeptideAtlasiQ8IZ07.
PRIDEiQ8IZ07.

PTM databases

iPTMnetiQ8IZ07.
PhosphoSiteiQ8IZ07.

Expressioni

Gene expression databases

BgeeiQ8IZ07.
CleanExiHS_ANKRD13A.
ExpressionAtlasiQ8IZ07. baseline and differential.
GenevisibleiQ8IZ07. HS.

Organism-specific databases

HPAiHPA039488.
HPA043218.

Interactioni

Subunit structurei

Interacts (via the UIM 3 and 4 repeats) with EGFR (ubiquitinated); the interaction is direct, inhibited by ANKRD13A monoubiquitination and may regulate EGFR internalization.1 Publication

Protein-protein interaction databases

BioGridi124582. 31 interactions.
IntActiQ8IZ07. 10 interactions.
MINTiMINT-1191854.
STRINGi9606.ENSP00000261739.

Structurei

3D structure databases

ProteinModelPortaliQ8IZ07.
SMRiQ8IZ07. Positions 6-96.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati40 – 6930ANK 1Add
BLAST
Repeati73 – 10230ANK 2Add
BLAST
Domaini483 – 50220UIM 1PROSITE-ProRule annotationAdd
BLAST
Domaini519 – 53820UIM 2PROSITE-ProRule annotationAdd
BLAST
Domaini549 – 56820UIM 3PROSITE-ProRule annotationAdd
BLAST
Domaini574 – 59017UIM 4PROSITE-ProRule annotationAdd
BLAST

Domaini

The UIM repeats 3 and 4 are required for binding to ubiquitinated EGFR and 'Lys-63'-linked ubiquitin.

Sequence similaritiesi

Contains 2 ANK repeats.PROSITE-ProRule annotation
Contains 4 UIM (ubiquitin-interacting motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0522. Eukaryota.
ENOG410XNMS. LUCA.
GeneTreeiENSGT00410000025381.
HOGENOMiHOG000230821.
HOVERGENiHBG059287.
InParanoidiQ8IZ07.
OMAiEKSEAIN.
OrthoDBiEOG76QFGX.
PhylomeDBiQ8IZ07.
TreeFamiTF314176.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR021832. ANKRD13.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR003903. UIM_dom.
[Graphical view]
PANTHERiPTHR12447. PTHR12447. 1 hit.
PfamiPF11904. GPCR_chapero_1. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
SM00726. UIM. 3 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50330. UIM. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8IZ07-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSACDAGDH YPLHLLVWKN DYRQLEKELQ GQNVEAVDPR GRTLLHLAVS
60 70 80 90 100
LGHLESARVL LRHKADVTKE NRQGWTVLHE AVSTGDPEMV YTVLQHRDYH
110 120 130 140 150
NTSMALEGVP ELLQKILEAP DFYVQMKWEF TSWVPLVSRI CPNDVCRIWK
160 170 180 190 200
SGAKLRVDIT LLGFENMSWI RGRRSFIFKG EDNWAELMEV NHDDKVVTTE
210 220 230 240 250
RFDLSQEMER LTLDLMKPKS REVERRLTSP VINTSLDTKN IAFERTKSGF
260 270 280 290 300
WGWRTDKAEV VNGYEAKVYT VNNVNVITKI RTEHLTEEEK KRYKADRNPL
310 320 330 340 350
ESLLGTVEHQ FGAQGDLTTE CATANNPTAI TPDEYFNEEF DLKDRDIGRP
360 370 380 390 400
KELTIRTQKF KAMLWMCEEF PLSLVEQVIP IIDLMARTSA HFARLRDFIK
410 420 430 440 450
LEFPPGFPVK IEIPLFHVLN ARITFGNVNG CSTAEESVSQ NVEGTQADSA
460 470 480 490 500
SHITNFEVDQ SVFEIPESYY VQDNGRNVHL QDEDYEIMQF AIQQSLLESS
510 520 530 540 550
RSQELSGPAS NGGISQTNTY DAQYERAIQE SLLTSTEGLC PSALSETSRF
560 570 580 590
DNDLQLAMEL SAKELEEWEL RLQEEEAELQ QVLQLSLTDK
Length:590
Mass (Da):67,619
Last modified:April 17, 2007 - v3
Checksum:i25D0B712D1A1B0B9
GO

Sequence cautioni

The sequence AAC17109.1 differs from that shown. Reason: Frameshift at positions 343 and 348. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti193 – 1931D → G in AAC17109 (Ref. 2) Curated
Sequence conflicti296 – 2961D → G in AAH32833 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti505 – 5051L → P.
Corresponds to variant rs2287174 [ dbSNP | Ensembl ].
VAR_048276

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC032833 mRNA. Translation: AAH32833.2.
AF064604 mRNA. Translation: AAC17109.1. Frameshift.
CCDSiCCDS9140.1.
RefSeqiNP_149112.1. NM_033121.1.
UniGeneiHs.528703.

Genome annotation databases

EnsembliENST00000261739; ENSP00000261739; ENSG00000076513.
GeneIDi88455.
KEGGihsa:88455.
UCSCiuc001tpx.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC032833 mRNA. Translation: AAH32833.2.
AF064604 mRNA. Translation: AAC17109.1. Frameshift.
CCDSiCCDS9140.1.
RefSeqiNP_149112.1. NM_033121.1.
UniGeneiHs.528703.

3D structure databases

ProteinModelPortaliQ8IZ07.
SMRiQ8IZ07. Positions 6-96.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124582. 31 interactions.
IntActiQ8IZ07. 10 interactions.
MINTiMINT-1191854.
STRINGi9606.ENSP00000261739.

PTM databases

iPTMnetiQ8IZ07.
PhosphoSiteiQ8IZ07.

Polymorphism and mutation databases

BioMutaiANKRD13A.
DMDMi145559439.

Proteomic databases

EPDiQ8IZ07.
MaxQBiQ8IZ07.
PaxDbiQ8IZ07.
PeptideAtlasiQ8IZ07.
PRIDEiQ8IZ07.

Protocols and materials databases

DNASUi88455.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261739; ENSP00000261739; ENSG00000076513.
GeneIDi88455.
KEGGihsa:88455.
UCSCiuc001tpx.4. human.

Organism-specific databases

CTDi88455.
GeneCardsiANKRD13A.
HGNCiHGNC:21268. ANKRD13A.
HPAiHPA039488.
HPA043218.
MIMi615123. gene.
neXtProtiNX_Q8IZ07.
PharmGKBiPA134884324.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0522. Eukaryota.
ENOG410XNMS. LUCA.
GeneTreeiENSGT00410000025381.
HOGENOMiHOG000230821.
HOVERGENiHBG059287.
InParanoidiQ8IZ07.
OMAiEKSEAIN.
OrthoDBiEOG76QFGX.
PhylomeDBiQ8IZ07.
TreeFamiTF314176.

Miscellaneous databases

ChiTaRSiANKRD13A. human.
GenomeRNAii88455.
PROiQ8IZ07.
SOURCEiSearch...

Gene expression databases

BgeeiQ8IZ07.
CleanExiHS_ANKRD13A.
ExpressionAtlasiQ8IZ07. baseline and differential.
GenevisibleiQ8IZ07. HS.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR021832. ANKRD13.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR003903. UIM_dom.
[Graphical view]
PANTHERiPTHR12447. PTHR12447. 1 hit.
PfamiPF11904. GPCR_chapero_1. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
SM00726. UIM. 3 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50330. UIM. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. "A novel gene from human dendritic cells."
    Zhao Z., Huang X., Li N., Zhu X., Cao X.
    Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-353.
    Tissue: Blood.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. "The Ankrd 13 family of UIM-bearing proteins regulates EGF receptor endocytosis from the plasma membrane."
    Tanno H., Yamaguchi T., Goto E., Ishido S., Komada M.
    Mol. Biol. Cell 23:1343-1353(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH EGFR, UBIQUITINATION, UIM DOMAIN, UBIQUITIN-BINDING, SUBCELLULAR LOCATION, MUTAGENESIS OF 491-ALA--SER-495; 527-ALA--SER-531; 557-ALA--SER-561 AND 582-VAL--SER-586.
  5. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-586, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiAN13A_HUMAN
AccessioniPrimary (citable) accession number: Q8IZ07
Secondary accession number(s): O60736
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: April 17, 2007
Last modified: July 6, 2016
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.