Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Izumo sperm-egg fusion protein 1

Gene

IZUMO1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB
  • receptor binding Source: UniProtKB

GO - Biological processi

  • cell adhesion Source: HGNC
  • fusion of sperm to egg plasma membrane Source: HGNC
  • multicellular organism reproduction Source: Reactome
  • single fertilization Source: UniProtKB
  • sperm-egg recognition Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Fertilization

Enzyme and pathway databases

ReactomeiR-HSA-1300652. Sperm:Oocyte Membrane Binding.

Protein family/group databases

TCDBi8.A.55.1.1. the izumo sperm-egg fusion protein 1 (izumo1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Izumo sperm-egg fusion protein 1
Alternative name(s):
Oocyte binding/fusion factor
Short name:
OBF
Sperm-specific protein izumo
Gene namesi
Name:IZUMO1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:28539. IZUMO1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 292271ExtracellularSequence analysisAdd
BLAST
Transmembranei293 – 31321HelicalSequence analysisAdd
BLAST
Topological domaini314 – 35037CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • acrosomal membrane Source: Ensembl
  • integral component of membrane Source: HGNC
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671647.

Polymorphism and mutation databases

BioMutaiIZUMO1.
DMDMi296434545.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 350329Izumo sperm-egg fusion protein 1PRO_0000045482Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi182 ↔ 233By similarity
Glycosylationi204 – 2041N-linked (GlcNAc...)Sequence analysis
Modified residuei325 – 3251PhosphoserineBy similarity

Post-translational modificationi

N-glycosylated. Glycosylation is not essential for fusion and for proper protein trafficking in sperm (By similarity).By similarity
Phosphorylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8IYV9.
PRIDEiQ8IYV9.

PTM databases

PhosphoSiteiQ8IYV9.

Expressioni

Tissue specificityi

Sperm-specific (at protein level). Detectable on sperm surface only after the acrosome reaction.1 Publication

Gene expression databases

BgeeiQ8IYV9.
CleanExiHS_IZUMO1.
ExpressionAtlasiQ8IYV9. baseline and differential.
GenevisibleiQ8IYV9. HS.

Organism-specific databases

HPAiHPA038104.

Interactioni

Subunit structurei

Monomer, homodimer and homooligomer. Interacts with IZUMO1R/JUNO (By similarity).By similarity

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB
  • receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi129843. 20 interactions.
STRINGi9606.ENSP00000327786.

Structurei

3D structure databases

ProteinModelPortaliQ8IYV9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini167 – 25185Ig-like C2-typeAdd
BLAST

Sequence similaritiesi

Belongs to the Izumo family.Curated

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHEV. Eukaryota.
ENOG410Z8SJ. LUCA.
GeneTreeiENSGT00390000015014.
HOGENOMiHOG000113123.
HOVERGENiHBG081812.
InParanoidiQ8IYV9.
OMAiKEVHACR.
OrthoDBiEOG72ZCG1.
PhylomeDBiQ8IYV9.
TreeFamiTF338356.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR029389. IZUMO.
IPR032699. Izumo-Ig.
IPR032700. IZUMO1.
[Graphical view]
PANTHERiPTHR35540:SF1. PTHR35540:SF1. 1 hit.
PfamiPF15005. IZUMO. 1 hit.
PF16706. Izumo-Ig. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8IYV9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGPHFTLLCA ALAGCLLPAE GCVICDPSVV LALKSLEKDY LPGHLDAKHH
60 70 80 90 100
KAMMERVENA VKDFQELSLN EDAYMGVVDE ATLQKGSWSL LKDLKRITDS
110 120 130 140 150
DVKGDLFVKE LFWMLHLQKE TFATYVARFQ KEAYCPNKCG VMLQTLIWCK
160 170 180 190 200
NCKKEVHACR KSYDCGERNV EVPQMEDMIL DCELNWHQAS EGLTDYSFYR
210 220 230 240 250
VWGNNTETLV SKGKEATLTK PMVGPEDAGS YRCELGSVNS SPATIINFHV
260 270 280 290 300
TVLPKMIKEE KPSPNIVTPG EATTESSISL QPLQPEKMLA SRLLGLLICG
310 320 330 340 350
SLALITGLTF AIFRRRKVID FIKSSLFGLG SGAAEQTQVP KEKATDSRQQ
Length:350
Mass (Da):38,930
Last modified:May 18, 2010 - v2
Checksum:i8741BB3F93ABCFA9
GO
Isoform 2 (identifier: Q8IYV9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     167-194: ERNVEVPQMEDMILDCELNWHQASEGLT → GERTGPSRRNWCRAECGSSSNGRHDPGL
     195-350: Missing.

Note: No experimental confirmation available.
Show »
Length:194
Mass (Da):21,758
Checksum:i9D56F3FC6B308080
GO
Isoform 3 (identifier: Q8IYV9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     57-68: VENAVKDFQELS → MRPHCKRGPGVC
     69-350: Missing.

Note: No experimental confirmation available.
Show »
Length:68
Mass (Da):7,339
Checksum:iA637C1EEBC33D4FE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti36 – 361L → P in AAS59145 (Ref. 2) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti333 – 3331A → V.3 Publications
Corresponds to variant rs2307019 [ dbSNP | Ensembl ].
VAR_055399

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei57 – 6812VENAV…FQELS → MRPHCKRGPGVC in isoform 3. 1 PublicationVSP_016960Add
BLAST
Alternative sequencei69 – 350282Missing in isoform 3. 1 PublicationVSP_016961Add
BLAST
Alternative sequencei167 – 19428ERNVE…SEGLT → GERTGPSRRNWCRAECGSSS NGRHDPGL in isoform 2. 1 PublicationVSP_016962Add
BLAST
Alternative sequencei195 – 350156Missing in isoform 2. 1 PublicationVSP_016963Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB195682 mRNA. Translation: BAD91012.1.
AY552609 mRNA. Translation: AAS59145.1.
AY552610 mRNA. Translation: AAS59146.1.
AC009002 Genomic DNA. No translation available.
BC034769 mRNA. Translation: AAH34769.1.
CCDSiCCDS12732.1. [Q8IYV9-1]
RefSeqiNP_872381.2. NM_182575.2. [Q8IYV9-1]
XP_011525114.1. XM_011526812.1. [Q8IYV9-1]
XP_011525115.1. XM_011526813.1. [Q8IYV9-1]
UniGeneiHs.400688.

Genome annotation databases

EnsembliENST00000332955; ENSP00000327786; ENSG00000182264. [Q8IYV9-1]
ENST00000595517; ENSP00000471815; ENSG00000182264. [Q8IYV9-3]
ENST00000595937; ENSP00000470144; ENSG00000182264. [Q8IYV9-2]
GeneIDi284359.
KEGGihsa:284359.
UCSCiuc002pkj.3. human. [Q8IYV9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB195682 mRNA. Translation: BAD91012.1.
AY552609 mRNA. Translation: AAS59145.1.
AY552610 mRNA. Translation: AAS59146.1.
AC009002 Genomic DNA. No translation available.
BC034769 mRNA. Translation: AAH34769.1.
CCDSiCCDS12732.1. [Q8IYV9-1]
RefSeqiNP_872381.2. NM_182575.2. [Q8IYV9-1]
XP_011525114.1. XM_011526812.1. [Q8IYV9-1]
XP_011525115.1. XM_011526813.1. [Q8IYV9-1]
UniGeneiHs.400688.

3D structure databases

ProteinModelPortaliQ8IYV9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi129843. 20 interactions.
STRINGi9606.ENSP00000327786.

Protein family/group databases

TCDBi8.A.55.1.1. the izumo sperm-egg fusion protein 1 (izumo1) family.

PTM databases

PhosphoSiteiQ8IYV9.

Polymorphism and mutation databases

BioMutaiIZUMO1.
DMDMi296434545.

Proteomic databases

PaxDbiQ8IYV9.
PRIDEiQ8IYV9.

Protocols and materials databases

DNASUi284359.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000332955; ENSP00000327786; ENSG00000182264. [Q8IYV9-1]
ENST00000595517; ENSP00000471815; ENSG00000182264. [Q8IYV9-3]
ENST00000595937; ENSP00000470144; ENSG00000182264. [Q8IYV9-2]
GeneIDi284359.
KEGGihsa:284359.
UCSCiuc002pkj.3. human. [Q8IYV9-1]

Organism-specific databases

CTDi284359.
GeneCardsiIZUMO1.
H-InvDBHIX0202883.
HGNCiHGNC:28539. IZUMO1.
HPAiHPA038104.
MIMi609278. gene.
neXtProtiNX_Q8IYV9.
PharmGKBiPA142671647.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IHEV. Eukaryota.
ENOG410Z8SJ. LUCA.
GeneTreeiENSGT00390000015014.
HOGENOMiHOG000113123.
HOVERGENiHBG081812.
InParanoidiQ8IYV9.
OMAiKEVHACR.
OrthoDBiEOG72ZCG1.
PhylomeDBiQ8IYV9.
TreeFamiTF338356.

Enzyme and pathway databases

ReactomeiR-HSA-1300652. Sperm:Oocyte Membrane Binding.

Miscellaneous databases

ChiTaRSiIZUMO1. human.
GenomeRNAii284359.
NextBioi94777.
PROiQ8IYV9.
SOURCEiSearch...

Gene expression databases

BgeeiQ8IYV9.
CleanExiHS_IZUMO1.
ExpressionAtlasiQ8IYV9. baseline and differential.
GenevisibleiQ8IYV9. HS.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR029389. IZUMO.
IPR032699. Izumo-Ig.
IPR032700. IZUMO1.
[Graphical view]
PANTHERiPTHR35540:SF1. PTHR35540:SF1. 1 hit.
PfamiPF15005. IZUMO. 1 hit.
PF16706. Izumo-Ig. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs."
    Inoue N., Ikawa M., Isotani A., Okabe M.
    Nature 434:234-238(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, VARIANT VAL-333.
    Tissue: Testis.
  2. Li H., Zhou G., Shen C., Ke R., Zhong G., Lin L., Yang S.
    Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
  3. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT VAL-333.
    Tissue: Brain.
  5. "Preliminary study on the role of the human IZUMO gene in oocyte-spermatozoa fusion failure."
    Granados-Gonzalez V., Aknin-Seifer I., Touraine R.-L., Chouteau J., Wolf J.-P., Levy R.
    Fertil. Steril. 90:1246-1248(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT VAL-333.

Entry informationi

Entry nameiIZUM1_HUMAN
AccessioniPrimary (citable) accession number: Q8IYV9
Secondary accession number(s): Q6Q8P6, Q6Q8P7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: May 18, 2010
Last modified: May 11, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Izumo is the name of a Japanese shrine to marriage.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.