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Protein

Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 2

Gene

CMTR2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that mediates mRNA cap2 2'-O-ribose methylation to the 5'-cap structure of mRNAs. Methylates the ribose of the second nucleotide of a m7GpppG-capped mRNA and small nuclear RNA (snRNA) (cap0) to produce m7GpppRmpNm (cap2). Recognizes a guanosine cap on RNA independently of its N7 methylation status. Display cap2 methylation on both cap0 and cap1. Displays a preference for cap1 RNAs.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-(2'-O-methyl-ribonucleotide)-[mRNA].1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei148S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation1
Binding sitei167S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation1
Binding sitei235S-adenosyl-L-methioninePROSITE-ProRule annotation1
Active sitei275Proton acceptor1 Publication1

GO - Molecular functioni

  • mRNA (nucleoside-2'-O-)-methyltransferase activity Source: UniProtKB

GO - Biological processi

  • 7-methylguanosine mRNA capping Source: UniProtKB
  • cap2 mRNA methylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciZFISH:ENSG00000180917-MONOMER.
BRENDAi2.1.1.296. 2681.

Names & Taxonomyi

Protein namesi
Recommended name:
Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 2 (EC:2.1.1.296)
Alternative name(s):
Cap methyltransferase 2
Cap2 2'O-ribose methyltransferase 2
Short name:
HMTr2
Short name:
MTr2
FtsJ methyltransferase domain-containing protein 1
Protein adrift homolog
Gene namesi
Name:CMTR2
Synonyms:AFT, FTSJD1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:25635. CMTR2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei642 – 662HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi117K → A: Abolishes catalytic activity. 1 Publication1
Mutagenesisi235D → A: Abolishes catalytic activity. 1 Publication1
Mutagenesisi275K → A: Abolishes catalytic activity. 1 Publication1

Organism-specific databases

DisGeNETi55783.
OpenTargetsiENSG00000180917.
PharmGKBiPA162389041.

Polymorphism and mutation databases

BioMutaiFTSJD1.
DMDMi269849596.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003261801 – 770Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 2Add BLAST770

Proteomic databases

EPDiQ8IYT2.
MaxQBiQ8IYT2.
PaxDbiQ8IYT2.
PeptideAtlasiQ8IYT2.
PRIDEiQ8IYT2.

PTM databases

iPTMnetiQ8IYT2.
PhosphoSitePlusiQ8IYT2.

Expressioni

Gene expression databases

BgeeiENSG00000180917.
CleanExiHS_FTSJD1.
ExpressionAtlasiQ8IYT2. baseline and differential.
GenevisibleiQ8IYT2. HS.

Organism-specific databases

HPAiHPA041700.
HPA048265.

Interactioni

Protein-protein interaction databases

BioGridi120897. 6 interactors.
STRINGi9606.ENSP00000337512.

Structurei

3D structure databases

ProteinModelPortaliQ8IYT2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini109 – 322Adrift-type SAM-dependent 2'-O-MTasePROSITE-ProRule annotationAdd BLAST214

Sequence similaritiesi

Contains 1 Adrift-type SAM-dependent 2'-O-MTase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3674. Eukaryota.
ENOG410XQ0Z. LUCA.
GeneTreeiENSGT00530000063742.
HOVERGENiHBG098139.
InParanoidiQ8IYT2.
KOiK14590.
OMAiLQFVPME.
OrthoDBiEOG091G0EIW.
PhylomeDBiQ8IYT2.
TreeFamiTF314897.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025807. Adrift-typ_MeTrfase.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01728. FtsJ. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51614. SAM_MT_ADRIFT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8IYT2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKCRKTPVQ QLASPASFSP DILADIFELF AKNFSYGKPL NNEWQLPDPS
60 70 80 90 100
EIFTCDHTEL NAFLDLKNSL NEVKNLLSDK KLDEWHEHTA FTNKAGKIIS
110 120 130 140 150
HVRKSVNAEL CTQAWCKFHE ILCSFPLIPQ EAFQNGKLNS LHLCEAPGAF
160 170 180 190 200
IASLNHYLKS HRFPCHWSWV ANTLNPYHEA NDDLMMIMDD RLIANTLHWW
210 220 230 240 250
YFGPDNTGDI MTLKFLTGLQ NFISSMATVH LVTADGSFDC QGNPGEQEAL
260 270 280 290 300
VSSLHYCEVV TALTTLGNGG SFVLKMFTMF EHCSINLMYL LNCCFDQVHV
310 320 330 340 350
FKPATSKAGN SEVYVVCLHY KGREAIHPLL SKMTLNFGTE MKRKALFPHH
360 370 380 390 400
VIPDSFLKRH EECCVFFHKY QLETISENIR LFECMGKAEQ EKLNNLRDCA
410 420 430 440 450
IQYFMQKFQL KHLSRNNWLV KKSSIGCSTN TKWFGQRNKY FKTYNERKML
460 470 480 490 500
EALSWKDKVA KGYFNSWAEE HGVYHPGQSS ILEGTASNLE CHLWHILEGK
510 520 530 540 550
KLPKVKCSPF CNGEILKTLN EAIEKSLGGA FNLDSKFRPK QQYSCSCHVF
560 570 580 590 600
SEELIFSELC SLTECLQDEQ VVVPSNQIKC LLVGFSTLRN IKMHIPLEVR
610 620 630 640 650
LLESAELTTF SCSLLHDGDP TYQRLFLDCL LHSLRELHTG DVMILPVLSC
660 670 680 690 700
FTRFMAGLIF VLHSCFRFIT FVCPTSSDPL RTCAVLLCVG YQDLPNPVFR
710 720 730 740 750
YLQSVNELLS TLLNSDSPQQ VLQFVPMEVL LKGALLDFLW DLNAAIAKRH
760 770
LHFIIQRERE EIINSLQLQN
Length:770
Mass (Da):88,120
Last modified:November 24, 2009 - v2
Checksum:iE5BE4A2B31AE3580
GO

Sequence cautioni

The sequence AAM49718 differs from that shown. Reason: Frameshift at position 337.Curated
The sequence BAA91058 differs from that shown. Reason: Frameshift at position 739.Curated
The sequence BAB14452 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27F → L in AAM49718 (Ref. 5) Curated1
Sequence conflicti53F → Y in AAM49718 (Ref. 5) Curated1
Sequence conflicti56D → E in AAM49718 (Ref. 5) Curated1
Sequence conflicti75N → K in AAM49718 (Ref. 5) Curated1
Sequence conflicti85 – 86WH → LD in AAM49718 (Ref. 5) Curated2
Sequence conflicti201 – 202YF → HL in AAM49718 (Ref. 5) Curated2
Sequence conflicti222F → I in AAM49718 (Ref. 5) Curated1
Sequence conflicti317C → S in AAM49718 (Ref. 5) Curated1
Sequence conflicti683C → S in BAA92047 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03999860L → F.2 PublicationsCorresponds to variant rs3096380dbSNPEnsembl.1
Natural variantiVAR_039999163F → Y.1 PublicationCorresponds to variant rs17853360dbSNPEnsembl.1
Natural variantiVAR_040000416N → S.1 PublicationCorresponds to variant rs3803704dbSNPEnsembl.1
Natural variantiVAR_040001608T → K.Corresponds to variant rs3096381dbSNPEnsembl.1
Natural variantiVAR_040002753F → L.Corresponds to variant rs16970857dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000289 mRNA. Translation: BAA91058.1. Frameshift.
AK002033 mRNA. Translation: BAA92047.1.
AK023183 mRNA. Translation: BAB14452.1. Different initiation.
AK315056 mRNA. Translation: BAG37532.1.
AC106736 Genomic DNA. No translation available.
CH471166 Genomic DNA. Translation: EAW59246.1.
CH471166 Genomic DNA. Translation: EAW59247.1.
BC034468 mRNA. Translation: AAH34468.1.
BC035005 mRNA. Translation: AAH35005.1.
AF458590 mRNA. Translation: AAM49718.1. Frameshift.
CCDSiCCDS10898.1.
RefSeqiNP_001093112.1. NM_001099642.1.
NP_001311303.1. NM_001324374.1.
NP_001311306.1. NM_001324377.1.
NP_001311307.1. NM_001324378.1.
NP_001311308.1. NM_001324379.1.
NP_060818.4. NM_018348.5.
XP_011521530.1. XM_011523228.1.
XP_011521531.1. XM_011523229.1.
XP_016878933.1. XM_017023444.1.
UniGeneiHs.72782.

Genome annotation databases

EnsembliENST00000338099; ENSP00000337512; ENSG00000180917.
ENST00000434935; ENSP00000411148; ENSG00000180917.
GeneIDi55783.
KEGGihsa:55783.
UCSCiuc002ezy.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000289 mRNA. Translation: BAA91058.1. Frameshift.
AK002033 mRNA. Translation: BAA92047.1.
AK023183 mRNA. Translation: BAB14452.1. Different initiation.
AK315056 mRNA. Translation: BAG37532.1.
AC106736 Genomic DNA. No translation available.
CH471166 Genomic DNA. Translation: EAW59246.1.
CH471166 Genomic DNA. Translation: EAW59247.1.
BC034468 mRNA. Translation: AAH34468.1.
BC035005 mRNA. Translation: AAH35005.1.
AF458590 mRNA. Translation: AAM49718.1. Frameshift.
CCDSiCCDS10898.1.
RefSeqiNP_001093112.1. NM_001099642.1.
NP_001311303.1. NM_001324374.1.
NP_001311306.1. NM_001324377.1.
NP_001311307.1. NM_001324378.1.
NP_001311308.1. NM_001324379.1.
NP_060818.4. NM_018348.5.
XP_011521530.1. XM_011523228.1.
XP_011521531.1. XM_011523229.1.
XP_016878933.1. XM_017023444.1.
UniGeneiHs.72782.

3D structure databases

ProteinModelPortaliQ8IYT2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120897. 6 interactors.
STRINGi9606.ENSP00000337512.

PTM databases

iPTMnetiQ8IYT2.
PhosphoSitePlusiQ8IYT2.

Polymorphism and mutation databases

BioMutaiFTSJD1.
DMDMi269849596.

Proteomic databases

EPDiQ8IYT2.
MaxQBiQ8IYT2.
PaxDbiQ8IYT2.
PeptideAtlasiQ8IYT2.
PRIDEiQ8IYT2.

Protocols and materials databases

DNASUi55783.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000338099; ENSP00000337512; ENSG00000180917.
ENST00000434935; ENSP00000411148; ENSG00000180917.
GeneIDi55783.
KEGGihsa:55783.
UCSCiuc002ezy.5. human.

Organism-specific databases

CTDi55783.
DisGeNETi55783.
GeneCardsiCMTR2.
HGNCiHGNC:25635. CMTR2.
HPAiHPA041700.
HPA048265.
MIMi616190. gene.
neXtProtiNX_Q8IYT2.
OpenTargetsiENSG00000180917.
PharmGKBiPA162389041.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3674. Eukaryota.
ENOG410XQ0Z. LUCA.
GeneTreeiENSGT00530000063742.
HOVERGENiHBG098139.
InParanoidiQ8IYT2.
KOiK14590.
OMAiLQFVPME.
OrthoDBiEOG091G0EIW.
PhylomeDBiQ8IYT2.
TreeFamiTF314897.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000180917-MONOMER.
BRENDAi2.1.1.296. 2681.

Miscellaneous databases

ChiTaRSiCMTR2. human.
GenomeRNAii55783.
PROiQ8IYT2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000180917.
CleanExiHS_FTSJD1.
ExpressionAtlasiQ8IYT2. baseline and differential.
GenevisibleiQ8IYT2. HS.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025807. Adrift-typ_MeTrfase.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01728. FtsJ. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51614. SAM_MT_ADRIFT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCMTR2_HUMAN
AccessioniPrimary (citable) accession number: Q8IYT2
Secondary accession number(s): B2RCD5
, D3DWS1, Q8NE77, Q8NFR5, Q9H8Z4, Q9NUS3, Q9NXF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: November 24, 2009
Last modified: November 2, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.