Q8IYL9 (PSYR_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Psychosine receptor

Alternative name(s):
G-protein coupled receptor 65
T-cell death-associated gene 8 protein

Gene names

Name:	GPR65
Synonyms:	TDAG8

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for the glycosphingolipid psychosine (PSY) and several related glycosphingolipids. May have a role in activation-induced cell death or differentiation of T-cells. Ref.4
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Predominantly expressed in thymus, spleen, lymph nodes, small intestine, lung, placenta and peripheral blood leukocytes. Ref.1 Ref.4
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin cytoskeleton reorganization Inferred from direct assay PubMed 15618224. Source: BHF-UCL activation of Rho GTPase activity Inferred from direct assay PubMed 15618224. Source: BHF-UCL apoptotic process Traceable author statement Ref.1. Source: ProtInc immune response Traceable author statement Ref.1. Source: ProtInc multicellular organismal development Traceable author statement Ref.1. Source: ProtInc positive regulation of cAMP biosynthetic process Inferred from direct assay PubMed 15618224. Source: BHF-UCL positive regulation of stress fiber assembly Inferred from direct assay PubMed 15618224. Source: BHF-UCL response to acidity Inferred from direct assay PubMed 15618224. Source: BHF-UCL
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	G-protein coupled receptor activity Inferred from direct assay PubMed 15618224. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Psychosine receptor		PRO_0000070087
Regions
	Topological domain	1 – 15	15	Extracellular Potential
	Transmembrane	16 – 36	21	Helical; Name=1; Potential
	Topological domain	37 – 50	14	Cytoplasmic Potential
	Transmembrane	51 – 71	21	Helical; Name=2; Potential
	Topological domain	72 – 89	18	Extracellular Potential
	Transmembrane	90 – 110	21	Helical; Name=3; Potential
	Topological domain	111 – 129	19	Cytoplasmic Potential
	Transmembrane	130 – 150	21	Helical; Name=4; Potential
	Topological domain	151 – 180	30	Extracellular Potential
	Transmembrane	181 – 201	21	Helical; Name=5; Potential
	Topological domain	202 – 227	26	Cytoplasmic Potential
	Transmembrane	228 – 248	21	Helical; Name=6; Potential
	Topological domain	249 – 273	25	Extracellular Potential
	Transmembrane	274 – 294	21	Helical; Name=7; Potential
	Topological domain	295 – 337	43	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	2	1	N-linked (GlcNAc...) Potential
	Glycosylation	79	1	N-linked (GlcNAc...) Potential
	Glycosylation	166	1	N-linked (GlcNAc...) Potential
	Disulfide bond	87 ↔ 170		By similarity
Natural variations
	Natural variant	231	1	I → L. Corresponds to variant rs3742704 [ dbSNP | Ensembl ].		VAR_022064
Experimental info
	Sequence conflict	43	1	A → P in AAC31794. Ref.1
	Sequence conflict	97	1	N → K in AAC31794. Ref.1
	Sequence conflict	130	1	F → I in AAC31794. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8IYL9 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: CFDBF217291C0FA2	FASTA	337	39,333
	10 20 30 40 50 60 MNSTCIEEQH DLDHYLFPIV YIFVIIVSIP ANIGSLCVSF LQAKKESELG IYLFSLSLSD 70 80 90 100 110 120 LLYALTLPLW IDYTWNKDNW TFSPALCKGS AFLMYMNFYS STAFLTCIAV DRYLAVVYPL 130 140 150 160 170 180 KFFFLRTRRF ALMVSLSIWI LETIFNAVML WEDETVVEYC DAEKSNFTLC YDKYPLEKWQ 190 200 210 220 230 240 INLNLFRTCT GYAIPLVTIL ICNRKVYQAV RHNKATENKE KKRIIKLLVS ITVTFVLCFT 250 260 270 280 290 300 PFHVMLLIRC ILEHAVNFED HSNSGKRTYT MYRITVALTS LNCVADPILY CFVTETGRYD 310 320 330 MWNILKFCTG RCNTSQRQRK RILSVSTKDT MELEVLE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, characterization, and mapping of human homolog of mouse T-cell death-associated gene." Kyaw H., Zeng Z., Su K., Fan P., Shell B.K., Carter K.C., Li Y. DNA Cell Biol. 17:493-500(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
[2]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Blood and Brain.
[4]	"Identification of a molecular target of psychosine and its role in globoid cell formation." Im D.-S., Heise C.E., Nguyen T., O'Dowd B.F., Lynch K.R. J. Cell Biol. 153:429-434(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U95218 Genomic DNA. Translation: AAC31794.1. AL157955 Genomic DNA. No translation available. BC035633 mRNA. Translation: AAH35633.1. BC071715 mRNA. Translation: AAH71715.1.
IPI	IPI00337428.
RefSeq	NP_003599.2. NM_003608.3.
UniGene	Hs.443243.
3D structure databases
ProteinModelPortal	Q8IYL9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8IYL9. 1 interaction.
STRING	9606.ENSP00000267549.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q8IYL9.
Polymorphism databases
DMDM	37537957.
Proteomic databases
PaxDb	Q8IYL9.
PRIDE	Q8IYL9.
Protocols and materials databases
DNASU	8477.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000267549; ENSP00000267549; ENSG00000140030.
GeneID	8477.
KEGG	hsa:8477.
UCSC	uc001xvv.3. human.
Organism-specific databases
CTD	8477.
GeneCards	GC14P088471.
HGNC	HGNC:4517. GPR65.
MIM	604620. gene.
neXtProt	NX_Q8IYL9.
PharmGKB	PA28909.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG149070.
HOGENOM	HOG000004801.
HOVERGEN	HBG101118.
InParanoid	Q8IYL9.
KO	K08416.
OMA	CYDKYPL.
OrthoDB	EOG4SJ5FB.
PhylomeDB	Q8IYL9.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q8IYL9.
Bgee	Q8IYL9.
CleanEx	HS_GPR65.
Genevestigator	Q8IYL9.
GermOnline	ENSG00000140030. Homo sapiens.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR005464. Psych_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01649. PSYCHOSINER.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	8477.
NextBio	31721.
SOURCE	Search...

Entry information

Entry name

PSYR_HUMAN

Accession

Primary (citable) accession number: Q8IYL9
Secondary accession number(s): O75819

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2003
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.