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Protein

Psychosine receptor

Gene

GPR65

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for the glycosphingolipid psychosine (PSY) and several related glycosphingolipids (PubMed:11309421). Plays a role in immune response by maintaining lysosome function and supporting phagocytosis-mediated intracellular bacteria clearance (PubMed:27287411). May have a role in activation-induced cell death or differentiation of T-cells (By similarity).By similarity2 Publications

GO - Molecular functioni

  • G-protein coupled receptor activity Source: BHF-UCL

GO - Biological processi

  • actin cytoskeleton reorganization Source: BHF-UCL
  • activation of GTPase activity Source: BHF-UCL
  • adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: BHF-UCL
  • apoptotic process Source: ProtInc
  • G-protein coupled receptor signaling pathway Source: BHF-UCL
  • immune response Source: ProtInc
  • multicellular organism development Source: ProtInc
  • positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway Source: GO_Central
  • positive regulation of Rho protein signal transduction Source: GO_Central
  • positive regulation of stress fiber assembly Source: BHF-UCL
  • response to acidic pH Source: BHF-UCL

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
Biological processApoptosis

Enzyme and pathway databases

ReactomeiR-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-416476 G alpha (q) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
Psychosine receptor
Alternative name(s):
G-protein coupled receptor 65
T-cell death-associated gene 8 protein
Gene namesi
Name:GPR65
Synonyms:TDAG8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000140030.5
HGNCiHGNC:4517 GPR65
MIMi604620 gene
neXtProtiNX_Q8IYL9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 15ExtracellularSequence analysisAdd BLAST15
Transmembranei16 – 36Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini37 – 50CytoplasmicSequence analysisAdd BLAST14
Transmembranei51 – 71Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini72 – 89ExtracellularSequence analysisAdd BLAST18
Transmembranei90 – 110Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini111 – 129CytoplasmicSequence analysisAdd BLAST19
Transmembranei130 – 150Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini151 – 180ExtracellularSequence analysisAdd BLAST30
Transmembranei181 – 201Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini202 – 227CytoplasmicSequence analysisAdd BLAST26
Transmembranei228 – 248Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini249 – 273ExtracellularSequence analysisAdd BLAST25
Transmembranei274 – 294Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini295 – 337CytoplasmicSequence analysisAdd BLAST43

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi8477
OpenTargetsiENSG00000140030
PharmGKBiPA28909

Chemistry databases

ChEMBLiCHEMBL3714081

Polymorphism and mutation databases

BioMutaiGPR65
DMDMi37537957

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000700871 – 337Psychosine receptorAdd BLAST337

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi2N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi79N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi87 ↔ 170PROSITE-ProRule annotation
Glycosylationi166N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8IYL9
PeptideAtlasiQ8IYL9
PRIDEiQ8IYL9

PTM databases

iPTMnetiQ8IYL9
PhosphoSitePlusiQ8IYL9

Expressioni

Tissue specificityi

Predominantly expressed in thymus, spleen, lymph nodes, small intestine, lung, placenta and peripheral blood leukocytes.2 Publications

Gene expression databases

BgeeiENSG00000140030
CleanExiHS_GPR65
ExpressionAtlasiQ8IYL9 baseline and differential
GenevisibleiQ8IYL9 HS

Organism-specific databases

HPAiHPA054454

Interactioni

Protein-protein interaction databases

IntActiQ8IYL9, 1 interactor
STRINGi9606.ENSP00000267549

Structurei

3D structure databases

ProteinModelPortaliQ8IYL9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00910000143980
HOGENOMiHOG000004801
HOVERGENiHBG101118
InParanoidiQ8IYL9
KOiK08416
OMAiCTGYAIP
OrthoDBiEOG091G0BK0
PhylomeDBiQ8IYL9
TreeFamiTF331803

Family and domain databases

InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR005464 Psych_rcpt
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR01649 PSYCHOSINER
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8IYL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSTCIEEQH DLDHYLFPIV YIFVIIVSIP ANIGSLCVSF LQAKKESELG
60 70 80 90 100
IYLFSLSLSD LLYALTLPLW IDYTWNKDNW TFSPALCKGS AFLMYMNFYS
110 120 130 140 150
STAFLTCIAV DRYLAVVYPL KFFFLRTRRF ALMVSLSIWI LETIFNAVML
160 170 180 190 200
WEDETVVEYC DAEKSNFTLC YDKYPLEKWQ INLNLFRTCT GYAIPLVTIL
210 220 230 240 250
ICNRKVYQAV RHNKATENKE KKRIIKLLVS ITVTFVLCFT PFHVMLLIRC
260 270 280 290 300
ILEHAVNFED HSNSGKRTYT MYRITVALTS LNCVADPILY CFVTETGRYD
310 320 330
MWNILKFCTG RCNTSQRQRK RILSVSTKDT MELEVLE
Length:337
Mass (Da):39,333
Last modified:March 1, 2003 - v1
Checksum:iCFDBF217291C0FA2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti43A → P in AAC31794 (PubMed:9655242).Curated1
Sequence conflicti97N → K in AAC31794 (PubMed:9655242).Curated1
Sequence conflicti130F → I in AAC31794 (PubMed:9655242).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_022064231I → L Polymorhism; increased lysosomal pH; increased lipid droplets accumulation. Corresponds to variant dbSNP:rs3742704Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U95218 Genomic DNA Translation: AAC31794.1
AL157955 Genomic DNA No translation available.
BC035633 mRNA Translation: AAH35633.1
BC071715 mRNA Translation: AAH71715.1
CCDSiCCDS9879.1
RefSeqiNP_003599.2, NM_003608.3
UniGeneiHs.443243

Genome annotation databases

EnsembliENST00000267549; ENSP00000267549; ENSG00000140030
GeneIDi8477
KEGGihsa:8477
UCSCiuc001xvv.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPSYR_HUMAN
AccessioniPrimary (citable) accession number: Q8IYL9
Secondary accession number(s): O75819
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: March 1, 2003
Last modified: February 28, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health