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Protein

Psychosine receptor

Gene

GPR65

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for the glycosphingolipid psychosine (PSY) and several related glycosphingolipids. May have a role in activation-induced cell death or differentiation of T-cells.1 Publication

GO - Molecular functioni

  • G-protein coupled receptor activity Source: BHF-UCL

GO - Biological processi

  • actin cytoskeleton reorganization Source: BHF-UCL
  • activation of GTPase activity Source: BHF-UCL
  • apoptotic process Source: ProtInc
  • G-protein coupled receptor signaling pathway Source: BHF-UCL
  • immune response Source: ProtInc
  • multicellular organismal development Source: ProtInc
  • positive regulation of cAMP biosynthetic process Source: BHF-UCL
  • positive regulation of stress fiber assembly Source: BHF-UCL
  • response to acidic pH Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

ReactomeiREACT_14828. Class A/1 (Rhodopsin-like receptors).
REACT_18283. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Psychosine receptor
Alternative name(s):
G-protein coupled receptor 65
T-cell death-associated gene 8 protein
Gene namesi
Name:GPR65
Synonyms:TDAG8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:4517. GPR65.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515ExtracellularSequence AnalysisAdd
BLAST
Transmembranei16 – 3621Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini37 – 5014CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei51 – 7121Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini72 – 8918ExtracellularSequence AnalysisAdd
BLAST
Transmembranei90 – 11021Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini111 – 12919CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei130 – 15021Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini151 – 18030ExtracellularSequence AnalysisAdd
BLAST
Transmembranei181 – 20121Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini202 – 22726CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei228 – 24821Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini249 – 27325ExtracellularSequence AnalysisAdd
BLAST
Transmembranei274 – 29421Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini295 – 33743CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: ProtInc
  • plasma membrane Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28909.

Polymorphism and mutation databases

BioMutaiGPR65.
DMDMi37537957.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 337337Psychosine receptorPRO_0000070087Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi2 – 21N-linked (GlcNAc...)Sequence Analysis
Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi87 ↔ 170PROSITE-ProRule annotation
Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8IYL9.
PRIDEiQ8IYL9.

PTM databases

PhosphoSiteiQ8IYL9.

Expressioni

Tissue specificityi

Predominantly expressed in thymus, spleen, lymph nodes, small intestine, lung, placenta and peripheral blood leukocytes.2 Publications

Gene expression databases

BgeeiQ8IYL9.
CleanExiHS_GPR65.
ExpressionAtlasiQ8IYL9. baseline and differential.
GenevestigatoriQ8IYL9.

Interactioni

Protein-protein interaction databases

IntActiQ8IYL9. 1 interaction.
STRINGi9606.ENSP00000267549.

Structurei

3D structure databases

ProteinModelPortaliQ8IYL9.
SMRiQ8IYL9. Positions 16-295.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG149070.
GeneTreeiENSGT00760000119001.
HOGENOMiHOG000004801.
HOVERGENiHBG101118.
InParanoidiQ8IYL9.
KOiK08416.
OMAiCYDKYPL.
OrthoDBiEOG7B31N7.
PhylomeDBiQ8IYL9.
TreeFamiTF331803.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR005464. Psych_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01649. PSYCHOSINER.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8IYL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSTCIEEQH DLDHYLFPIV YIFVIIVSIP ANIGSLCVSF LQAKKESELG
60 70 80 90 100
IYLFSLSLSD LLYALTLPLW IDYTWNKDNW TFSPALCKGS AFLMYMNFYS
110 120 130 140 150
STAFLTCIAV DRYLAVVYPL KFFFLRTRRF ALMVSLSIWI LETIFNAVML
160 170 180 190 200
WEDETVVEYC DAEKSNFTLC YDKYPLEKWQ INLNLFRTCT GYAIPLVTIL
210 220 230 240 250
ICNRKVYQAV RHNKATENKE KKRIIKLLVS ITVTFVLCFT PFHVMLLIRC
260 270 280 290 300
ILEHAVNFED HSNSGKRTYT MYRITVALTS LNCVADPILY CFVTETGRYD
310 320 330
MWNILKFCTG RCNTSQRQRK RILSVSTKDT MELEVLE
Length:337
Mass (Da):39,333
Last modified:March 1, 2003 - v1
Checksum:iCFDBF217291C0FA2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti43 – 431A → P in AAC31794 (PubMed:9655242).Curated
Sequence conflicti97 – 971N → K in AAC31794 (PubMed:9655242).Curated
Sequence conflicti130 – 1301F → I in AAC31794 (PubMed:9655242).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti231 – 2311I → L.
Corresponds to variant rs3742704 [ dbSNP | Ensembl ].
VAR_022064

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U95218 Genomic DNA. Translation: AAC31794.1.
AL157955 Genomic DNA. No translation available.
BC035633 mRNA. Translation: AAH35633.1.
BC071715 mRNA. Translation: AAH71715.1.
CCDSiCCDS9879.1.
RefSeqiNP_003599.2. NM_003608.3.
UniGeneiHs.443243.

Genome annotation databases

EnsembliENST00000267549; ENSP00000267549; ENSG00000140030.
GeneIDi8477.
KEGGihsa:8477.
UCSCiuc001xvv.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U95218 Genomic DNA. Translation: AAC31794.1.
AL157955 Genomic DNA. No translation available.
BC035633 mRNA. Translation: AAH35633.1.
BC071715 mRNA. Translation: AAH71715.1.
CCDSiCCDS9879.1.
RefSeqiNP_003599.2. NM_003608.3.
UniGeneiHs.443243.

3D structure databases

ProteinModelPortaliQ8IYL9.
SMRiQ8IYL9. Positions 16-295.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8IYL9. 1 interaction.
STRINGi9606.ENSP00000267549.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ8IYL9.

Polymorphism and mutation databases

BioMutaiGPR65.
DMDMi37537957.

Proteomic databases

PaxDbiQ8IYL9.
PRIDEiQ8IYL9.

Protocols and materials databases

DNASUi8477.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000267549; ENSP00000267549; ENSG00000140030.
GeneIDi8477.
KEGGihsa:8477.
UCSCiuc001xvv.3. human.

Organism-specific databases

CTDi8477.
GeneCardsiGC14P088471.
HGNCiHGNC:4517. GPR65.
MIMi604620. gene.
neXtProtiNX_Q8IYL9.
PharmGKBiPA28909.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG149070.
GeneTreeiENSGT00760000119001.
HOGENOMiHOG000004801.
HOVERGENiHBG101118.
InParanoidiQ8IYL9.
KOiK08416.
OMAiCYDKYPL.
OrthoDBiEOG7B31N7.
PhylomeDBiQ8IYL9.
TreeFamiTF331803.

Enzyme and pathway databases

ReactomeiREACT_14828. Class A/1 (Rhodopsin-like receptors).
REACT_18283. G alpha (q) signalling events.

Miscellaneous databases

GeneWikiiGPR65.
GenomeRNAii8477.
NextBioi31721.
PROiQ8IYL9.
SOURCEiSearch...

Gene expression databases

BgeeiQ8IYL9.
CleanExiHS_GPR65.
ExpressionAtlasiQ8IYL9. baseline and differential.
GenevestigatoriQ8IYL9.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR005464. Psych_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01649. PSYCHOSINER.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, characterization, and mapping of human homolog of mouse T-cell death-associated gene."
    Kyaw H., Zeng Z., Su K., Fan P., Shell B.K., Carter K.C., Li Y.
    DNA Cell Biol. 17:493-500(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
  2. "The DNA sequence and analysis of human chromosome 14."
    Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
    , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
    Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Blood and Brain.
  4. "Identification of a molecular target of psychosine and its role in globoid cell formation."
    Im D.-S., Heise C.E., Nguyen T., O'Dowd B.F., Lynch K.R.
    J. Cell Biol. 153:429-434(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiPSYR_HUMAN
AccessioniPrimary (citable) accession number: Q8IYL9
Secondary accession number(s): O75819
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: March 1, 2003
Last modified: April 29, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.