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Protein

Exocyst complex component 8

Gene

EXOC8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.

GO - Molecular functioni

  • Ral GTPase binding Source: UniProtKB

GO - Biological processi

  • cellular protein metabolic process Source: Reactome
  • endosome organization Source: UniProtKB
  • exocyst assembly Source: GO_Central
  • exocyst localization Source: GO_Central
  • extracellular matrix disassembly Source: UniProtKB
  • membrane organization Source: Reactome
  • organelle organization Source: Reactome
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_147867. Translocation of GLUT4 to the plasma membrane.
REACT_15550. Insulin processing.
REACT_268299. VxPx cargo-targeting to cilium.

Names & Taxonomyi

Protein namesi
Recommended name:
Exocyst complex component 8
Alternative name(s):
Exocyst complex 84 kDa subunit
Gene namesi
Name:EXOC8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:24659. EXOC8.

Subcellular locationi

  • Cytoplasm
  • Cell projectiongrowth cone By similarity

  • Note: Redistributes to growing neurites and growth cones during cell differentiation (By similarity). Binds lipids with phosphatidylinositol 3,4,5-trisphosphate groups.By similarity

GO - Cellular componenti

  • cytoplasm Source: HPA
  • cytosol Source: Reactome
  • exocyst Source: UniProtKB
  • growth cone Source: UniProtKB-SubCell
  • late endosome Source: UniProtKB
  • membrane Source: UniProtKB
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134991382.

Polymorphism and mutation databases

BioMutaiEXOC8.
DMDMi74750763.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 725725Exocyst complex component 8PRO_0000227550Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei142 – 1421Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8IYI6.
PaxDbiQ8IYI6.
PRIDEiQ8IYI6.

PTM databases

PhosphoSiteiQ8IYI6.

Expressioni

Gene expression databases

BgeeiQ8IYI6.
CleanExiHS_EXOC8.
GenevisibleiQ8IYI6. HS.

Organism-specific databases

HPAiHPA027438.

Interactioni

Subunit structurei

The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8. Interacts with the GTP-bound forms of RALA and RALB via its PH domain.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
BECN1Q144574EBI-742102,EBI-949378
CEP57L1Q8IYX8-23EBI-742102,EBI-10181988
IKZF3Q9UKT93EBI-742102,EBI-747204
KRT31Q153233EBI-742102,EBI-948001
MBIPQ9NS73-53EBI-742102,EBI-10182361
USHBP1Q8N6Y02EBI-742102,EBI-739895
WASH1A8K0Z33EBI-742102,EBI-6160405

Protein-protein interaction databases

BioGridi127206. 41 interactions.
IntActiQ8IYI6. 25 interactions.
MINTiMINT-1449731.
STRINGi9606.ENSP00000353564.

Structurei

3D structure databases

ProteinModelPortaliQ8IYI6.
SMRiQ8IYI6. Positions 180-292.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini182 – 282101PHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi108 – 11710Poly-Ala
Compositional biasi316 – 3194Poly-Glu

Sequence similaritiesi

Belongs to the EXO84 family.Curated
Contains 1 PH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG331886.
GeneTreeiENSGT00390000015936.
HOGENOMiHOG000006737.
HOVERGENiHBG081489.
InParanoidiQ8IYI6.
OMAiRNFEQYT.
OrthoDBiEOG7NSB1Q.
PhylomeDBiQ8IYI6.
TreeFamiTF105819.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR016159. Cullin_repeat-like_dom.
IPR011993. PH-like_dom.
IPR001849. PH_domain.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF74788. SSF74788. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8IYI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMAMSDSGA SRLRRQLESG GFEARLYVKQ LSQQSDGDRD LQEHRQRIQA
60 70 80 90 100
LAEETAQNLK RNVYQNYRQF IETAREISYL ESEMYQLSHL LTEQKSSLES
110 120 130 140 150
IPLTLLPAAA AAGAAAASGG EEGVGGAGGR DHLRGQAGFF STPGGASRDG
160 170 180 190 200
SGPGEEGKQR TLTTLLEKVE GCRHLLETPG QYLVYNGDLV EYDADHMAQL
210 220 230 240 250
QRVHGFLMND CLLVATWLPQ RRGMYRYNAL YSLDGLAVVN VKDNPPMKDM
260 270 280 290 300
FKLLMFPESR IFQAENAKIK REWLEVLEDT KRALSEKRRR EQEEAAAPRG
310 320 330 340 350
PPQVTSKATN PFEDDEEEEP AVPEVEEEKV DLSMEWIQEL PEDLDVCIAQ
360 370 380 390 400
RDFEGAVDLL DKLNHYLEDK PSPPPVKELR AKVEERVRQL TEVLVFELSP
410 420 430 440 450
DRSLRGGPKA TRRAVSQLIR LGQCTKACEL FLRNRAAAVH TAIRQLRIEG
460 470 480 490 500
ATLLYIHKLC HVFFTSLLET AREFEIDFAG TDSGCYSAFV VWARSAMGMF
510 520 530 540 550
VDAFSKQVFD SKESLSTAAE CVKVAKEHCQ QLGDIGLDLT FIIHALLVKD
560 570 580 590 600
IQGALHSYKE IIIEATKHRN SEEMWRRMNL MTPEALGKLK EEMKSCGVSN
610 620 630 640 650
FEQYTGDDCW VNLSYTVVAF TKQTMGFLEE ALKLYFPELH MVLLESLVEI
660 670 680 690 700
ILVAVQHVDY SLRCEQDPEK KAFIRQNASF LYETVLPVVE KRFEEGVGKP
710 720
AKQLQDLRNA SRLIRVNPES TTSVV
Length:725
Mass (Da):81,799
Last modified:July 5, 2004 - v2
Checksum:iF4E5E344EF24FDCA
GO

Sequence cautioni

The sequence CAI23095.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK096460 mRNA. Translation: BAG53295.1.
AL117352 Genomic DNA. Translation: CAI23095.1. Different initiation.
CH471098 Genomic DNA. Translation: EAW69955.1.
BC035763 mRNA. Translation: AAH35763.2.
CCDSiCCDS1593.1.
RefSeqiNP_787072.2. NM_175876.4.
UniGeneiHs.356198.
Hs.554892.

Genome annotation databases

EnsembliENST00000366645; ENSP00000355605; ENSG00000116903.
GeneIDi149371.
KEGGihsa:149371.
UCSCiuc001huq.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK096460 mRNA. Translation: BAG53295.1.
AL117352 Genomic DNA. Translation: CAI23095.1. Different initiation.
CH471098 Genomic DNA. Translation: EAW69955.1.
BC035763 mRNA. Translation: AAH35763.2.
CCDSiCCDS1593.1.
RefSeqiNP_787072.2. NM_175876.4.
UniGeneiHs.356198.
Hs.554892.

3D structure databases

ProteinModelPortaliQ8IYI6.
SMRiQ8IYI6. Positions 180-292.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127206. 41 interactions.
IntActiQ8IYI6. 25 interactions.
MINTiMINT-1449731.
STRINGi9606.ENSP00000353564.

PTM databases

PhosphoSiteiQ8IYI6.

Polymorphism and mutation databases

BioMutaiEXOC8.
DMDMi74750763.

Proteomic databases

MaxQBiQ8IYI6.
PaxDbiQ8IYI6.
PRIDEiQ8IYI6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000366645; ENSP00000355605; ENSG00000116903.
GeneIDi149371.
KEGGihsa:149371.
UCSCiuc001huq.3. human.

Organism-specific databases

CTDi149371.
GeneCardsiGC01M231469.
HGNCiHGNC:24659. EXOC8.
HPAiHPA027438.
MIMi615283. gene.
neXtProtiNX_Q8IYI6.
PharmGKBiPA134991382.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG331886.
GeneTreeiENSGT00390000015936.
HOGENOMiHOG000006737.
HOVERGENiHBG081489.
InParanoidiQ8IYI6.
OMAiRNFEQYT.
OrthoDBiEOG7NSB1Q.
PhylomeDBiQ8IYI6.
TreeFamiTF105819.

Enzyme and pathway databases

ReactomeiREACT_147867. Translocation of GLUT4 to the plasma membrane.
REACT_15550. Insulin processing.
REACT_268299. VxPx cargo-targeting to cilium.

Miscellaneous databases

GeneWikiiEXOC8.
GenomeRNAii149371.
NextBioi86129.
PROiQ8IYI6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8IYI6.
CleanExiHS_EXOC8.
GenevisibleiQ8IYI6. HS.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR016159. Cullin_repeat-like_dom.
IPR011993. PH-like_dom.
IPR001849. PH_domain.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF74788. SSF74788. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Tongue.
  2. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lymph.
  5. "Ral GTPases regulate exocyst assembly through dual subunit interactions."
    Moskalenko S., Tong C., Rosse C., Mirey G., Formstecher E., Daviet L., Camonis J., White M.A.
    J. Biol. Chem. 278:51743-51748(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EXOC2; RALA AND RALB.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-142, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiEXOC8_HUMAN
AccessioniPrimary (citable) accession number: Q8IYI6
Secondary accession number(s): B3KU33, Q5TE82
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: July 5, 2004
Last modified: July 22, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.