Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Eukaryotic peptide chain release factor GTP-binding subunit ERF3B

Gene

GSPT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in translation termination in response to the termination codons UAA, UAG and UGA. May play a role as a potent stimulator of the release factor activity of ETF1. Exhibits GTPase activity, which is ribosome- and ETF1-dependent. May play a role in cell cycle progression. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei216Interacts with GTP/GDPBy similarity1
Sitei350Interacts with GTP/GDPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi210 – 217GTPBy similarity8
Nucleotide bindingi287 – 291GTPBy similarity5
Nucleotide bindingi349 – 352GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, Nonsense-mediated mRNA decay, Protein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-72764. Eukaryotic Translation Termination.
R-HSA-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-HSA-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic peptide chain release factor GTP-binding subunit ERF3B
Short name:
Eukaryotic peptide chain release factor subunit 3b
Short name:
eRF3b
Alternative name(s):
G1 to S phase transition protein 2 homolog
Gene namesi
Name:GSPT2
Synonyms:ERF3B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:4622. GSPT2.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi52L → K: Impairs interaction with UPF1 and PABPC1; when associated with A-55, K-56 and A-59. 1 Publication1
Mutagenesisi55N → A: Impairs interaction with UPF1 and PABPC1; when associated with K-52, K-56 and A-59. 1 Publication1
Mutagenesisi56A → K: Impairs interaction with UPF1 and PABPC1; when associated with K52, A-55, and A-59. 1 Publication1
Mutagenesisi59F → A: Impairs interaction with UPF1 and PABPC1; when associated with K-52, A-55 and K-56. 1 Publication1

Organism-specific databases

DisGeNETi23708.
OpenTargetsiENSG00000189369.
PharmGKBiPA29013.

Polymorphism and mutation databases

BioMutaiGSPT2.
DMDMi182647413.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003272561 – 628Eukaryotic peptide chain release factor GTP-binding subunit ERF3BAdd BLAST628

Proteomic databases

EPDiQ8IYD1.
MaxQBiQ8IYD1.
PaxDbiQ8IYD1.
PeptideAtlasiQ8IYD1.
PRIDEiQ8IYD1.

PTM databases

iPTMnetiQ8IYD1.
PhosphoSitePlusiQ8IYD1.
SwissPalmiQ8IYD1.

Expressioni

Tissue specificityi

Highly expressed in IUCC stage II colorectal cancer (CRC).1 Publication

Gene expression databases

BgeeiENSG00000189369.
CleanExiHS_GSPT2.
GenevisibleiQ8IYD1. HS.

Organism-specific databases

HPAiHPA044769.

Interactioni

Subunit structurei

Interacts with ETF1 (By similarity). Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. Interacts with UPF1 and PABPC1.By similarity2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi117221. 46 interactors.
IntActiQ8IYD1. 6 interactors.
MINTiMINT-4994700.
STRINGi9606.ENSP00000341247.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KUJX-ray1.40B59-73[»]
ProteinModelPortaliQ8IYD1.
SMRiQ8IYD1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8IYD1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini201 – 425tr-type GPROSITE-ProRule annotationAdd BLAST225

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni210 – 217G1PROSITE-ProRule annotation8
Regioni266 – 270G2PROSITE-ProRule annotation5
Regioni287 – 290G3PROSITE-ProRule annotation4
Regioni349 – 352G4PROSITE-ProRule annotation4
Regioni391 – 393G5PROSITE-ProRule annotation3

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. ERF3 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0459. Eukaryota.
COG5256. LUCA.
GeneTreeiENSGT00620000087924.
HOGENOMiHOG000229291.
HOVERGENiHBG000179.
InParanoidiQ8IYD1.
KOiK03267.
OMAiIRKSKYM.
OrthoDBiEOG093709FE.
PhylomeDBiQ8IYD1.
TreeFamiTF300566.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiView protein in InterPro
IPR009818. Ataxin-2_C.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004160. Transl_elong_EFTu/EF1A_C.
PfamiView protein in Pfam
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
PF07145. PAM2. 1 hit.
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8IYD1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSGSSSSDS APDCWDQVDM ESPGSAPSGD GVSSAVAEAQ REPLSSAFSR
60 70 80 90 100
KLNVNAKPFV PNVHAAEFVP SFLRGPTQPP TLPAGSGSND ETCTGAGYPQ
110 120 130 140 150
GKRMGRGAPV EPSREEPLVS LEGSNSAVTM ELSEPVVENG EVEMALEESW
160 170 180 190 200
EHSKEVSEAE PGGGSSGDSG PPEESGQEMM EEKEEIRKSK SVIVPSGAPK
210 220 230 240 250
KEHVNVVFIG HVDAGKSTIG GQIMFLTGMV DKRTLEKYER EAKEKNRETW
260 270 280 290 300
YLSWALDTNQ EERDKGKTVE VGRAYFETER KHFTILDAPG HKSFVPNMIG
310 320 330 340 350
GASQADLAVL VISARKGEFE TGFEKGGQTR EHAMLAKTAG VKHLIVLINK
360 370 380 390 400
MDDPTVNWSI ERYEECKEKL VPFLKKVGFS PKKDIHFMPC SGLTGANIKE
410 420 430 440 450
QSDFCPWYTG LPFIPYLDNL PNFNRSIDGP IRLPIVDKYK DMGTVVLGKL
460 470 480 490 500
ESGSIFKGQQ LVMMPNKHNV EVLGILSDDT ETDFVAPGEN LKIRLKGIEE
510 520 530 540 550
EEILPGFILC DPSNLCHSGR TFDVQIVIIE HKSIICPGYN AVLHIHTCIE
560 570 580 590 600
EVEITALISL VDKKSGEKSK TRPRFVKQDQ VCIARLRTAG TICLETFKDF
610 620
PQMGRFTLRD EGKTIAIGKV LKLVPEKD
Length:628
Mass (Da):68,883
Last modified:April 8, 2008 - v2
Checksum:i909FCDEF04EC89C9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25S → L in BAA91612 (PubMed:14702039).Curated1
Sequence conflicti119V → A in BAA91612 (PubMed:14702039).Curated1
Sequence conflicti293S → G in BAA91612 (PubMed:14702039).Curated1
Sequence conflicti354P → H in AAH36077 (PubMed:15489334).Curated1
Sequence conflicti625P → Q in AAH36077 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04243123P → T1 PublicationCorresponds to variant dbSNP:rs17855593Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ251548 mRNA. Translation: CAB91089.1.
AK001303 mRNA. Translation: BAA91612.1.
AL929101 Genomic DNA. Translation: CAH71524.1.
CH471230 Genomic DNA. Translation: EAW62898.1.
BC036077 mRNA. Translation: AAH36077.1.
CCDSiCCDS14336.1.
RefSeqiNP_060564.2. NM_018094.4.
UniGeneiHs.59523.

Genome annotation databases

EnsembliENST00000340438; ENSP00000341247; ENSG00000189369.
GeneIDi23708.
KEGGihsa:23708.
UCSCiuc004dpl.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ251548 mRNA. Translation: CAB91089.1.
AK001303 mRNA. Translation: BAA91612.1.
AL929101 Genomic DNA. Translation: CAH71524.1.
CH471230 Genomic DNA. Translation: EAW62898.1.
BC036077 mRNA. Translation: AAH36077.1.
CCDSiCCDS14336.1.
RefSeqiNP_060564.2. NM_018094.4.
UniGeneiHs.59523.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KUJX-ray1.40B59-73[»]
ProteinModelPortaliQ8IYD1.
SMRiQ8IYD1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117221. 46 interactors.
IntActiQ8IYD1. 6 interactors.
MINTiMINT-4994700.
STRINGi9606.ENSP00000341247.

PTM databases

iPTMnetiQ8IYD1.
PhosphoSitePlusiQ8IYD1.
SwissPalmiQ8IYD1.

Polymorphism and mutation databases

BioMutaiGSPT2.
DMDMi182647413.

Proteomic databases

EPDiQ8IYD1.
MaxQBiQ8IYD1.
PaxDbiQ8IYD1.
PeptideAtlasiQ8IYD1.
PRIDEiQ8IYD1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000340438; ENSP00000341247; ENSG00000189369.
GeneIDi23708.
KEGGihsa:23708.
UCSCiuc004dpl.4. human.

Organism-specific databases

CTDi23708.
DisGeNETi23708.
GeneCardsiGSPT2.
H-InvDBHIX0023147.
HGNCiHGNC:4622. GSPT2.
HPAiHPA044769.
MIMi300418. gene.
neXtProtiNX_Q8IYD1.
OpenTargetsiENSG00000189369.
PharmGKBiPA29013.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0459. Eukaryota.
COG5256. LUCA.
GeneTreeiENSGT00620000087924.
HOGENOMiHOG000229291.
HOVERGENiHBG000179.
InParanoidiQ8IYD1.
KOiK03267.
OMAiIRKSKYM.
OrthoDBiEOG093709FE.
PhylomeDBiQ8IYD1.
TreeFamiTF300566.

Enzyme and pathway databases

ReactomeiR-HSA-72764. Eukaryotic Translation Termination.
R-HSA-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-HSA-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

ChiTaRSiGSPT2. human.
EvolutionaryTraceiQ8IYD1.
GeneWikiiGSPT2.
GenomeRNAii23708.
PROiQ8IYD1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000189369.
CleanExiHS_GSPT2.
GenevisibleiQ8IYD1. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiView protein in InterPro
IPR009818. Ataxin-2_C.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004160. Transl_elong_EFTu/EF1A_C.
PfamiView protein in Pfam
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
PF07145. PAM2. 1 hit.
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiERF3B_HUMAN
AccessioniPrimary (citable) accession number: Q8IYD1
Secondary accession number(s): Q9H909, Q9NVY0, Q9NY44
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 8, 2008
Last modified: February 15, 2017
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.