Reviewed, UniProtKB/Swiss-Prot Q8IYB5 (SMAP1_HUMAN)

Names and origin

Protein names

Recommended name:
    Stromal membrane-associated protein 1

Gene names

Name:

SMAP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	GTPase activating protein that acts on ARF6. Plays a role in clathrin-dependent endocytosis. May play a role in erythropoiesis By similarity.
Subunit structure	Interacts with ARF6. Interacts with clathrin heavy chains via the clathrin box-like motif By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity.
Tissue specificity	Detected in bone marrow, adrenal gland, trachea, lymph node, spinal cord, peripheral blood leukocytes, thyroid and stomach. Ref.1
Sequence similarities	Contains 1 Arf-GAP domain.
Sequence caution	The sequence AAH08672.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	GTPase activation
Technical term	3D-structure
Gene Ontology (GO)
Biological process	regulation of ARF GTPase activity Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ARF GTPase activator activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8IYB5-1)

Also known as: SMAP1A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8IYB5-2)

Also known as: SMAP1B;

The sequence of this isoform differs from the canonical sequence as follows:
     139-165: Missing.

Isoform 3 (identifier: Q8IYB5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     139-165: Missing.
     424-467: MNQQMAGMSI...GQTLSTQLWK → IMQKGDAVLQ...ESADGWHEYQ

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	Stromal membrane-associated protein 1		PRO_0000235838
Regions
	Domain	18 – 136	119	Arf-GAP
	Zinc finger	33 – 56	24	C4-type
	Motif	218 – 222	5	Interaction with clathrin heavy chains By similarity
Natural variations
	Alternative sequence	139 – 165	27	Missing in isoform 2 and isoform 3.		VSP_018502
	Alternative sequence	424 – 467	44	MNQQM…TQLWK → IMQKGDAVLQHSISAIYWPM TRWLKCPLVDESADGWHEYQ in isoform 3.		VSP_018503
	Natural variant	212	1	A → V: dbSNP rs2273566. Ref.4		VAR_048326
Experimental info
	Sequence conflict	394	1	P → H in AAH36123. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (SMAP1A) [UniParc]. Last modified May 16, 2006. Version 2. Checksum: 75935CB6F9F99DA9	FASTA	467	50,386
	10 20 30 40 50 60 MATRSCREKA QKLNEQHQLI LSKLLREEDN KYCADCEAKG PRWASWNIGV FICIRCAGIH 70 80 90 100 110 120 RNLGVHISRV KSVNLDQWTA EQIQCMQDMG NTKARLLYEA NLPENFRRPQ TDQAVEFFIR 130 140 150 160 170 180 DKYEKKKYYD KNAIAITNIS SSDAPLQPLV SSPSLQAAVD KNKLEKEKEK KKEEKKREKE 190 200 210 220 230 240 PEKPAKPLTA EKLQKKDQQL EPKKSTSPKK AAEPTVDLLG LDGPAVAPVT NGNTTVPPLN 250 260 270 280 290 300 DDLDIFGPMI SNPLPATVMP PAQGTPSAPA AATLSTVTSG DLDLFTEQTT KSEEVAKKQL 310 320 330 340 350 360 SKDSILSLYG TGTIQQQSTP GVFMGPTNIP FTSQAPAAFQ GFPSMGVPVP AAPGLIGNVM 370 380 390 400 410 420 GQSPSMMVGM PMPNGFMGNA QTGVMPLPQN VVGPQGGMVG QMGAPQSKFG LPQAQQPQWS 430 440 450 460 LSQMNQQMAG MSISSATPTA GFGQPSSTTA GWSGSSSGQT LSTQLWK
	Isoform 2 (SMAP1B). Checksum: B7D7AE5387DB9E25	FASTA	440	47,609
	10 20 30 40 50 60 MATRSCREKA QKLNEQHQLI LSKLLREEDN KYCADCEAKG PRWASWNIGV FICIRCAGIH 70 80 90 100 110 120 RNLGVHISRV KSVNLDQWTA EQIQCMQDMG NTKARLLYEA NLPENFRRPQ TDQAVEFFIR 130 140 150 160 170 180 DKYEKKKYYD KNAIAITNKE KEKKKEEKKR EKEPEKPAKP LTAEKLQKKD QQLEPKKSTS 190 200 210 220 230 240 PKKAAEPTVD LLGLDGPAVA PVTNGNTTVP PLNDDLDIFG PMISNPLPAT VMPPAQGTPS 250 260 270 280 290 300 APAAATLSTV TSGDLDLFTE QTTKSEEVAK KQLSKDSILS LYGTGTIQQQ STPGVFMGPT 310 320 330 340 350 360 NIPFTSQAPA AFQGFPSMGV PVPAAPGLIG NVMGQSPSMM VGMPMPNGFM GNAQTGVMPL 370 380 390 400 410 420 PQNVVGPQGG MVGQMGAPQS KFGLPQAQQP QWSLSQMNQQ MAGMSISSAT PTAGFGQPSS 430 440 TTAGWSGSSS GQTLSTQLWK
	Isoform 3. Checksum: B4C4E780E6BEDCED	FASTA	436	47,862
	10 20 30 40 50 60 MATRSCREKA QKLNEQHQLI LSKLLREEDN KYCADCEAKG PRWASWNIGV FICIRCAGIH 70 80 90 100 110 120 RNLGVHISRV KSVNLDQWTA EQIQCMQDMG NTKARLLYEA NLPENFRRPQ TDQAVEFFIR 130 140 150 160 170 180 DKYEKKKYYD KNAIAITNKE KEKKKEEKKR EKEPEKPAKP LTAEKLQKKD QQLEPKKSTS 190 200 210 220 230 240 PKKAAEPTVD LLGLDGPAVA PVTNGNTTVP PLNDDLDIFG PMISNPLPAT VMPPAQGTPS 250 260 270 280 290 300 APAAATLSTV TSGDLDLFTE QTTKSEEVAK KQLSKDSILS LYGTGTIQQQ STPGVFMGPT 310 320 330 340 350 360 NIPFTSQAPA AFQGFPSMGV PVPAAPGLIG NVMGQSPSMM VGMPMPNGFM GNAQTGVMPL 370 380 390 400 410 420 PQNVVGPQGG MVGQMGAPQS KFGLPQAQQP QWSLSQIMQK GDAVLQHSIS AIYWPMTRWL 430 KCPLVDESAD GWHEYQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, characterization and chromosome mapping of the human SMAP1 gene." Marcos I., Borrego S., Rodriguez de Cordoba S., Galan J.J., Antinolo G. Gene 292:167-171(2002) [PubMed: 12119110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY. Tissue: Blood and Bone marrow.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[3]	Guo J.H., Zan Q., Yu L. Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT VAL-212. Tissue: Colon.
[5]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Ovary.
[7]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 248-467. Tissue: Testis.
[8]	"Solution structure of ARFGAP domain from human SMAP1." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 9-136 IN COMPLEX WITH ZINC IONS.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases

EMBLGenBankDDBJ

AY055003 mRNA. Translation: AAL14714.1.
AY055004 mRNA. Translation: AAL14715.1.
AY055015 , AY055005, AY055006, AY055007, AY055008, AY055009, AY055010, AY055011, AY055012, AY055013, AY055014 Genomic DNA. Translation: AAL14716.1.
AY055015 , AY055005, AY055006, AY055007, AY055008, AY055010, AY055011, AY055012, AY055013, AY055014 Genomic DNA. Translation: AAL14717.1.
AK023221 mRNA. Translation: BAB14473.1.
AF442495 mRNA. Translation: AAP97320.1.
AK222711 mRNA. Translation: BAD96431.1.
AL354943, AL591049, AL121961 Genomic DNA. Translation: CAI12310.1.
AL354943, AL121961, AL591049 Genomic DNA. Translation: CAI12311.1.
AL354943, AL121961, AL591049 Genomic DNA. Translation: CAI12312.1.
AL591049, AL121961, AL354943 Genomic DNA. Translation: CAI14240.1.
AL591049, AL121961, AL354943 Genomic DNA. Translation: CAI14241.1.
AL591049, AL121961, AL354943 Genomic DNA. Translation: CAI14242.1.
AL121961, AL591049, AL354943 Genomic DNA. Translation: CAI42146.1.
AL121961, AL354943, AL591049 Genomic DNA. Translation: CAI42147.1.
AL121961, AL354943, AL591049 Genomic DNA. Translation: CAI42148.1.
BC008672 mRNA. Translation: AAH08672.1. Sequence problems.
BC028074 mRNA. Translation: AAH28074.1.
BC036123 mRNA. Translation: AAH36123.1.
AL833906 mRNA. Translation: CAD38762.1.

IPI

IPI00102096.
IPI00185325.
IPI00604487.

RefSeq

NP_001037770.1.
NP_068759.2.

UniGene

Hs.485717

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CRR	NMR	-	A	9-136	[»]

ModBase

Search...

Proteomic databases

PRIDE

Q8IYB5.

Genome annotation databases

Ensembl

ENSG00000112305. Homo sapiens. [Contig view]

GeneID

60682.

KEGG

hsa:60682.

Organism-specific databases

GeneCards

GC06P071434.

H-InvDB

HIX0005999.

HGNC

HGNC:19651. SMAP1.

MIM

611372. gene.

PharmGKB

PA134893764.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

Q8IYB5.

OMA

Q8IYB5. ASMMVGM.

Gene expression databases

ArrayExpress

Q8IYB5.

Bgee

Q8IYB5.

CleanEx

HS_SMAP1.

GermOnline

ENSG00000112305. Homo sapiens.

Family and domain databases

InterPro

IPR001164. ArfGAP.
[Graphical view]

Pfam

PF01412. ArfGap. 1 hit.
[Graphical view]

PRINTS

PR00405. REVINTRACTNG.

SMART

SM00105. ArfGap. 1 hit.
[Graphical view]

PROSITE

PS50115. ARFGAP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

65508.

SOURCE

Search...

Entry information

Entry name

SMAP1_HUMAN

Accession

Primary (citable) accession number: Q8IYB5
Secondary accession number(s): Q53H70 , Q5SYQ2, Q6PK24, Q8NDH4, Q96L38, Q96L39, Q9H8X4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	May 16, 2006
Last modified:	June 16, 2009
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)