Q8IYB5 (SMAP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Stromal membrane-associated protein 1

Gene names

Name:

SMAP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	GTPase activating protein that acts on ARF6. Plays a role in clathrin-dependent endocytosis. May play a role in erythropoiesis By similarity.
Subunit structure	Interacts with ARF6. Interacts with clathrin heavy chains via the clathrin box-like motif By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity.
Tissue specificity	Detected in bone marrow, adrenal gland, trachea, lymph node, spinal cord, peripheral blood leukocytes, thyroid and stomach. Ref.1
Sequence similarities	Contains 1 Arf-GAP domain.
Sequence caution	The sequence AAH08672.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	GTPase activation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of ARF GTPase activity Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ARF GTPase activator activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8IYB5-1)

Also known as: SMAP1A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8IYB5-2)

Also known as: SMAP1B;

The sequence of this isoform differs from the canonical sequence as follows:
     139-165: Missing.

Isoform 3 (identifier: Q8IYB5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     139-165: Missing.
     424-467: MNQQMAGMSI...GQTLSTQLWK → IMQKGDAVLQ...ESADGWHEYQ

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 467

467

Stromal membrane-associated protein 1

PRO_0000235838

Regions

Domain

18 – 136

119

Arf-GAP

Zinc finger

33 – 56

24

C4-type

Motif

218 – 222

5

Interaction with clathrin heavy chains By similarity

Natural variations

Alternative sequence

139 – 165

27

Missing in isoform 2 and isoform 3.

VSP_018502

Alternative sequence

424 – 467

44

MNQQM…TQLWK → IMQKGDAVLQHSISAIYWPM TRWLKCPLVDESADGWHEYQ in isoform 3.

VSP_018503

Natural variant

212

1

A → V. Ref.4
Corresponds to variant rs2273566 [ dbSNP | Ensembl ].

VAR_048326

Experimental info

Sequence conflict

394

1

P → H in AAH36123. Ref.6

Secondary structure

1

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467

Helix Strand Turn

Details...

Turn

10 – 15

6

Helix

17 – 26

10

Helix

28 – 30

3

Beta strand

34 – 36

3

Turn

54 – 56

3

Turn

60 – 62

3

Beta strand

73 – 76

4

Helix

80 – 88

9

Helix

92 – 97

6

Helix

98 – 100

3

Helix

112 – 123

12

Helix

132 – 134

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (SMAP1A) [UniParc]. Last modified May 16, 2006. Version 2. Checksum: 75935CB6F9F99DA9	FASTA	467	50,386
	10 20 30 40 50 60 MATRSCREKA QKLNEQHQLI LSKLLREEDN KYCADCEAKG PRWASWNIGV FICIRCAGIH 70 80 90 100 110 120 RNLGVHISRV KSVNLDQWTA EQIQCMQDMG NTKARLLYEA NLPENFRRPQ TDQAVEFFIR 130 140 150 160 170 180 DKYEKKKYYD KNAIAITNIS SSDAPLQPLV SSPSLQAAVD KNKLEKEKEK KKEEKKREKE 190 200 210 220 230 240 PEKPAKPLTA EKLQKKDQQL EPKKSTSPKK AAEPTVDLLG LDGPAVAPVT NGNTTVPPLN 250 260 270 280 290 300 DDLDIFGPMI SNPLPATVMP PAQGTPSAPA AATLSTVTSG DLDLFTEQTT KSEEVAKKQL 310 320 330 340 350 360 SKDSILSLYG TGTIQQQSTP GVFMGPTNIP FTSQAPAAFQ GFPSMGVPVP AAPGLIGNVM 370 380 390 400 410 420 GQSPSMMVGM PMPNGFMGNA QTGVMPLPQN VVGPQGGMVG QMGAPQSKFG LPQAQQPQWS 430 440 450 460 LSQMNQQMAG MSISSATPTA GFGQPSSTTA GWSGSSSGQT LSTQLWK
	Isoform 2 (SMAP1B) [UniParc]. Checksum: B7D7AE5387DB9E25	FASTA	440	47,609
	10 20 30 40 50 60 MATRSCREKA QKLNEQHQLI LSKLLREEDN KYCADCEAKG PRWASWNIGV FICIRCAGIH 70 80 90 100 110 120 RNLGVHISRV KSVNLDQWTA EQIQCMQDMG NTKARLLYEA NLPENFRRPQ TDQAVEFFIR 130 140 150 160 170 180 DKYEKKKYYD KNAIAITNKE KEKKKEEKKR EKEPEKPAKP LTAEKLQKKD QQLEPKKSTS 190 200 210 220 230 240 PKKAAEPTVD LLGLDGPAVA PVTNGNTTVP PLNDDLDIFG PMISNPLPAT VMPPAQGTPS 250 260 270 280 290 300 APAAATLSTV TSGDLDLFTE QTTKSEEVAK KQLSKDSILS LYGTGTIQQQ STPGVFMGPT 310 320 330 340 350 360 NIPFTSQAPA AFQGFPSMGV PVPAAPGLIG NVMGQSPSMM VGMPMPNGFM GNAQTGVMPL 370 380 390 400 410 420 PQNVVGPQGG MVGQMGAPQS KFGLPQAQQP QWSLSQMNQQ MAGMSISSAT PTAGFGQPSS 430 440 TTAGWSGSSS GQTLSTQLWK
	Isoform 3 [UniParc]. Checksum: B4C4E780E6BEDCED	FASTA	436	47,862
	10 20 30 40 50 60 MATRSCREKA QKLNEQHQLI LSKLLREEDN KYCADCEAKG PRWASWNIGV FICIRCAGIH 70 80 90 100 110 120 RNLGVHISRV KSVNLDQWTA EQIQCMQDMG NTKARLLYEA NLPENFRRPQ TDQAVEFFIR 130 140 150 160 170 180 DKYEKKKYYD KNAIAITNKE KEKKKEEKKR EKEPEKPAKP LTAEKLQKKD QQLEPKKSTS 190 200 210 220 230 240 PKKAAEPTVD LLGLDGPAVA PVTNGNTTVP PLNDDLDIFG PMISNPLPAT VMPPAQGTPS 250 260 270 280 290 300 APAAATLSTV TSGDLDLFTE QTTKSEEVAK KQLSKDSILS LYGTGTIQQQ STPGVFMGPT 310 320 330 340 350 360 NIPFTSQAPA AFQGFPSMGV PVPAAPGLIG NVMGQSPSMM VGMPMPNGFM GNAQTGVMPL 370 380 390 400 410 420 PQNVVGPQGG MVGQMGAPQS KFGLPQAQQP QWSLSQIMQK GDAVLQHSIS AIYWPMTRWL 430 KCPLVDESAD GWHEYQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, characterization and chromosome mapping of the human SMAP1 gene." Marcos I., Borrego S., Rodriguez de Cordoba S., Galan J.J., Antinolo G. Gene 292:167-171(2002) [PubMed: 12119110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY. Tissue: Blood and Bone marrow.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[3]	Guo J.H., Zan Q., Yu L. Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT VAL-212. Tissue: Colon.
[5]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Ovary.
[7]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 248-467. Tissue: Testis.
[8]	"Solution structure of ARFGAP domain from human SMAP1." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 9-136 IN COMPLEX WITH ZINC IONS.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY055003 mRNA. Translation: AAL14714.1.
AY055004 mRNA. Translation: AAL14715.1.
AY055015 , AY055005, AY055006, AY055007, AY055008, AY055009, AY055010, AY055011, AY055012, AY055013, AY055014 Genomic DNA. Translation: AAL14716.1.
AY055015 , AY055005, AY055006, AY055007, AY055008, AY055010, AY055011, AY055012, AY055013, AY055014 Genomic DNA. Translation: AAL14717.1.
AK023221 mRNA. Translation: BAB14473.1.
AF442495 mRNA. Translation: AAP97320.1.
AK222711 mRNA. Translation: BAD96431.1.
AL354943, AL591049, AL121961 Genomic DNA. Translation: CAI12310.1.
AL354943, AL121961, AL591049 Genomic DNA. Translation: CAI12311.1.
AL354943, AL121961, AL591049 Genomic DNA. Translation: CAI12312.1.
AL591049, AL121961, AL354943 Genomic DNA. Translation: CAI14240.1.
AL591049, AL121961, AL354943 Genomic DNA. Translation: CAI14241.1.
AL591049, AL121961, AL354943 Genomic DNA. Translation: CAI14242.1.
AL121961, AL591049, AL354943 Genomic DNA. Translation: CAI42146.1.
AL121961, AL354943, AL591049 Genomic DNA. Translation: CAI42147.1.
AL121961, AL354943, AL591049 Genomic DNA. Translation: CAI42148.1.
BC008672 mRNA. Translation: AAH08672.1. Sequence problems.
BC028074 mRNA. Translation: AAH28074.1.
BC036123 mRNA. Translation: AAH36123.1.
AL833906 mRNA. Translation: CAD38762.1.

IPI

IPI00102096.
IPI00185325.
IPI00604487.

RefSeq

NP_001037770.1. NM_001044305.1.
NP_068759.2. NM_021940.3.

UniGene

Hs.485717.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CRR	NMR	-	A	9-136	[»]

ProteinModelPortal

Q8IYB5.

SMR

Q8IYB5. Positions 9-141.

ModBase

Search...

Protein-protein interaction databases

STRING

Q8IYB5.

PTM databases

PhosphoSite

Q8IYB5.

Polymorphism databases

DMDM

97190718.

Proteomic databases

PRIDE

Q8IYB5.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000370455; ENSP00000359484; ENSG00000112305.

GeneID

60682.

KEGG

hsa:60682.

UCSC

uc003pfr.1. human.
uc003pfs.1. human.
uc010kao.1. human.

Organism-specific databases

CTD

60682.

GeneCards

GC06P071377.

H-InvDB

HIX0005999.

HGNC

HGNC:19651. SMAP1.

HPA

HPA030574.

MIM

611372. gene.

neXtProt

NX_Q8IYB5.

PharmGKB

PA134893764.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG18336.

GeneTree

ENSGT00530000062917.

HOVERGEN

HBG055260.

InParanoid

Q8IYB5.

OMA

AQQPQWS.

PhylomeDB

Q8IYB5.

Gene expression databases

ArrayExpress

Q8IYB5.

Bgee

Q8IYB5.

CleanEx

HS_SMAP1.

Genevestigator

Q8IYB5.

GermOnline

ENSG00000112305. Homo sapiens.

Family and domain databases

InterPro

IPR001164. ArfGAP.
[Graphical view]

KO

K12486.

Pfam

PF01412. ArfGap. 1 hit.
[Graphical view]

PRINTS

PR00405. REVINTRACTNG.

SMART

SM00105. ArfGap. 1 hit.
[Graphical view]

SUPFAM

SSF57863. ArfGAP. 1 hit.

PROSITE

PS50115. ARFGAP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

65508.

SOURCE

Search...

Entry information

Entry name

SMAP1_HUMAN

Accession

Primary (citable) accession number: Q8IYB5
Secondary accession number(s): Q53H70 , Q5SYQ2, Q6PK24, Q8NDH4, Q96L38, Q96L39, Q9H8X4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	May 16, 2006
Last modified:	January 25, 2012
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.