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Q8IY84 (NIM1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase NIM1

EC=2.7.11.1
Alternative name(s):
NIM1 serine/threonine-protein kinase
Gene names
Name:NIM1K
Synonyms:NIM1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length436 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. Ref.1

Cofactor

Magnesium. Ref.1

Enzyme regulation

Activated by phosphorylation at Thr-229, probably by autophosphorylation. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 436436Serine/threonine-protein kinase NIM1
PRO_0000247666

Regions

Domain74 – 325252Protein kinase
Nucleotide binding80 – 889ATP By similarity UniProtKB P28523

Sites

Active site1961Proton acceptor By similarity UniProtKB P28523
Binding site1031ATP By similarity UniProtKB P28523

Amino acid modifications

Modified residue2291Phosphothreonine; by autocatalysis Ref.1

Natural variations

Natural variant211R → W. Ref.6
VAR_040945
Natural variant641E → Q. Ref.6
VAR_040946
Natural variant2601L → I. Ref.6
VAR_040947
Natural variant3201M → I. Ref.6
VAR_040948
Natural variant3331P → S in a lung neuroendocrine carcinoma sample; somatic mutation. Ref.6
VAR_040949
Natural variant4111P → T in a lung large cell carcinoma sample; somatic mutation. Ref.6
VAR_040950

Experimental info

Mutagenesis2291T → A: Loss of autophosphorylation and kinase activity. Ref.1
Mutagenesis2291T → E: Constitutively active. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8IY84 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: C183F11F54E3B87C

FASTA43649,606
        10         20         30         40         50         60 
MTAVYMNGGG LVNPHYARWD RRDSVESGCQ TESSKEGEEG QPRQLTPFEK LTQDMSQDEK 

        70         80         90        100        110        120 
VVREITLGKR IGFYRIRGEI GSGNFSQVKL GIHSLTKEKV AIKILDKTKL DQKTQRLLSR 

       130        140        150        160        170        180 
EISSMEKLHH PNIIRLYEVV ETLSKLHLVM EYAGGGELFG KISTEGKLSE PESKLIFSQI 

       190        200        210        220        230        240 
VSAVKHMHEN QIIHRDLKAE NVFYTSNTCV KVGDFGFSTV SKKGEMLNTF CGSPPYAAPE 

       250        260        270        280        290        300 
LFRDEHYIGI YVDIWALGVL LYFMVTGTMP FRAETVAKLK KSILEGTYSV PPHVSEPCHR 

       310        320        330        340        350        360 
LIRGVLQQIP TERYGIDCIM NDEWMQGVPY PTPLEPFQLD PKHLSETSTL KEEENEVKST 

       370        380        390        400        410        420 
LEHLGITEEH IRNNQGRDAR SSITGVYRII LHRVQRKKAL ESVPVMMLPD PKERDLKKGS 

       430 
RVYRGIRHTS KFCSIL 

« Hide

References

« Hide 'large scale' references
[1]"Identification of the sucrose non-fermenting related kinase SNRK, as a novel LKB1 substrate."
Jaleel M., McBride A., Lizcano J.M., Deak M., Toth R., Morrice N.A., Alessi D.R.
FEBS Lett. 579:1417-1423(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, PHOSPHORYLATION AT THR-229, MUTAGENESIS OF THR-229.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Amygdala.
[3]"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. expand/collapse author list , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[6]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] TRP-21; GLN-64; ILE-260; ILE-320; SER-333 AND THR-411.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK122985 mRNA. Translation: BAG53833.1.
AC106800 Genomic DNA. No translation available.
AC114947 Genomic DNA. No translation available.
CH471119 Genomic DNA. Translation: EAW56053.1.
BC036422 mRNA. Translation: AAH36422.1.
RefSeqNP_699192.1. NM_153361.3.
UniGeneHs.25845.

3D structure databases

ProteinModelPortalQ8IY84.
SMRQ8IY84. Positions 24-390.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid127945. 1 interaction.

Chemistry

BindingDBQ8IY84.
ChEMBLCHEMBL3542.
GuidetoPHARMACOLOGY2291.

PTM databases

PhosphoSiteQ8IY84.

Polymorphism databases

DMDM74759697.

Proteomic databases

PaxDbQ8IY84.
PRIDEQ8IY84.

Protocols and materials databases

DNASU167359.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID167359.
KEGGhsa:167359.
UCSCuc003jno.4. human.

Organism-specific databases

CTD167359.
GeneCardsGC05P043193.
HGNCHGNC:28646. NIM1K.
HPAHPA007695.
neXtProtNX_Q8IY84.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233016.
HOVERGENHBG082029.
InParanoidQ8IY84.
KOK16310.
OMAVKHMHEN.
PhylomeDBQ8IY84.

Enzyme and pathway databases

SignaLinkQ8IY84.

Gene expression databases

BgeeQ8IY84.
GenevestigatorQ8IY84.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi167359.
NextBio88667.
PROQ8IY84.

Entry information

Entry nameNIM1_HUMAN
AccessionPrimary (citable) accession number: Q8IY84
Secondary accession number(s): B3KVM1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM