Q8IY63 (AMOL1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Angiomotin-like protein 1

Gene names

Name:

AMOTL1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	956 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting CTNNB1 to recycling endosomes and hence preventing its translocation to the nucleus. Ref.12
Subcellular location	Cell junction › tight junction By similarity.
Post-translational modification	Polyubiquitinated by NEDD4, leading to proteasomal degradation.
Sequence similarities	Belongs to the angiomotin family.
Sequence caution	The sequence BAB84910.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAB84910.1 differs from that shown. Reason: Frameshift at positions 592 and 944.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Cell junction Tight junction
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW establishment of cell polarity involved in ameboidal cell migration Inferred from electronic annotation. Source: Compara hippo signaling cascade Traceable author statement. Source: Reactome positive regulation of blood vessel endothelial cell migration Inferred from electronic annotation. Source: Compara
Cellular_component	apical plasma membrane Inferred from electronic annotation. Source: Compara cytoplasmic vesicle Inferred from electronic annotation. Source: Compara cytosol Traceable author statement. Source: Reactome lamellipodium Inferred from electronic annotation. Source: Compara tight junction Inferred from direct assay PubMed 16019084. Source: MGI
Molecular_function	identical protein binding Inferred from direct assay PubMed 16019084. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8IY63-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8IY63-2)

The sequence of this isoform differs from the canonical sequence as follows:
     17-66: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 956	956	Angiomotin-like protein 1		PRO_0000190670
Regions
	Coiled coil	259 – 279	21	Potential
	Coiled coil	438 – 639	202	Potential
	Coiled coil	665 – 694	30	Potential
	Coiled coil	729 – 762	34	Potential
	Motif	953 – 956	4	PDZ-binding
Amino acid modifications
	Modified residue	241	1	Phosphoserine Ref.9
	Modified residue	295	1	Phosphoserine Ref.9
	Modified residue	720	1	Phosphoserine Ref.9
	Modified residue	793	1	Phosphoserine By similarity
	Modified residue	805	1	Phosphoserine By similarity
	Modified residue	906	1	Phosphoserine Ref.9
Natural variations
	Alternative sequence	17 – 66	50	Missing in isoform 2.		VSP_015710
	Natural variant	847	1	P → L. Corresponds to variant rs11020968 [ dbSNP | Ensembl ].		VAR_033498
Experimental info
	Sequence conflict	459 – 460	2	LQ → HE in AAH14126. Ref.4
	Sequence conflict	649	1	K → E in CAH56180. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 6F47AE3A13816E95	FASTA	956	106,574
	10 20 30 40 50 60 MWRAKLRRGT CEPAVKGSPS ACYSPSSPVQ VLEDSTYFSP DFQLYSGRHE TSALTVEATS 70 80 90 100 110 120 SIREKVVEDP LCNFHSPNFL RISEVEMRGS EDAAAGTVLQ RLIQEQLRYG TPTENMNLLA 130 140 150 160 170 180 IQHQATGSAG PAHPTNNFSS TENLTQEDPQ MVYQSARQEP QGQEHQVDNT VMEKQVRSTQ 190 200 210 220 230 240 PQQNNEELPT YEEAKAQSQF FRGQQQQQQQ QGAVGHGYYM AGGTSQKSRT EGRPTVNRAN 250 260 270 280 290 300 SGQAHKDEAL KELKQGHVRS LSERIMQLSL ERNGAKQHLP GSGNGKGFKV GGGPSPAQPA 310 320 330 340 350 360 GKVLDPRGPP PEYPFKTKQM MSPVSKTQEH GLFYGDQHPG MLHEMVKPYP APQPVRTDVA 370 380 390 400 410 420 VLRYQPPPEY GVTSRPCQLP FPSTMQQHSP MSSQTSSASG PLHSVSLPLP LPMALGAPQP 430 440 450 460 470 480 PPAASPSQQL GPDAFAIVER AQQMVEILTE ENRVLHQELQ GYYDNADKLH KFEKELQRIS 490 500 510 520 530 540 EAYESLVKST TKRESLDKAM RNKLEGEIRR LHDFNRDLRD RLETANRQLS SREYEGHEDK 550 560 570 580 590 600 AAEGHYASQN KEFLKEKEKL EMELAAVRTA SEDHRRHIEI LDQALSNAQA RVIKLEEELR 610 620 630 640 650 660 EKQAYVEKVE KLQQALTQLQ SACEKREQME RRLRTWLERE LDALRTQQKH GNGQPANMPE 670 680 690 700 710 720 YNAPALLELV REKEERILAL EADMTKWEQK YLEESTIRHF AMNAAATAAA ERDTTIINHS 730 740 750 760 770 780 RNGSYGESSL EAHIWQEEEE VVQANRRCQD MEYTIKNLHA KIIEKDAMIK VLQQRSRKDA 790 800 810 820 830 840 GKTDSSSLRP ARSVPSIAAA TGTHSRQTSL TSSQLAEEKK EEKTWKGSIG LLLGKEHHEH 850 860 870 880 890 900 ASAPLLPPPP TSALSSIAST TAASSAHAKT GSKDSSTQTD KSAELFWPSM ASLPSRGRLS 910 920 930 940 950 TTPAHSPVLK HPAAKGTAEK LENSPGHGKS PDHRGRVSSL LHKPEFPDGE MMEVLI
	Isoform 2 [UniParc]. Checksum: 8CFA1AE879538953	FASTA	906	101,179
	10 20 30 40 50 60 MWRAKLRRGT CEPAVKVEDP LCNFHSPNFL RISEVEMRGS EDAAAGTVLQ RLIQEQLRYG 70 80 90 100 110 120 TPTENMNLLA IQHQATGSAG PAHPTNNFSS TENLTQEDPQ MVYQSARQEP QGQEHQVDNT 130 140 150 160 170 180 VMEKQVRSTQ PQQNNEELPT YEEAKAQSQF FRGQQQQQQQ QGAVGHGYYM AGGTSQKSRT 190 200 210 220 230 240 EGRPTVNRAN SGQAHKDEAL KELKQGHVRS LSERIMQLSL ERNGAKQHLP GSGNGKGFKV 250 260 270 280 290 300 GGGPSPAQPA GKVLDPRGPP PEYPFKTKQM MSPVSKTQEH GLFYGDQHPG MLHEMVKPYP 310 320 330 340 350 360 APQPVRTDVA VLRYQPPPEY GVTSRPCQLP FPSTMQQHSP MSSQTSSASG PLHSVSLPLP 370 380 390 400 410 420 LPMALGAPQP PPAASPSQQL GPDAFAIVER AQQMVEILTE ENRVLHQELQ GYYDNADKLH 430 440 450 460 470 480 KFEKELQRIS EAYESLVKST TKRESLDKAM RNKLEGEIRR LHDFNRDLRD RLETANRQLS 490 500 510 520 530 540 SREYEGHEDK AAEGHYASQN KEFLKEKEKL EMELAAVRTA SEDHRRHIEI LDQALSNAQA 550 560 570 580 590 600 RVIKLEEELR EKQAYVEKVE KLQQALTQLQ SACEKREQME RRLRTWLERE LDALRTQQKH 610 620 630 640 650 660 GNGQPANMPE YNAPALLELV REKEERILAL EADMTKWEQK YLEESTIRHF AMNAAATAAA 670 680 690 700 710 720 ERDTTIINHS RNGSYGESSL EAHIWQEEEE VVQANRRCQD MEYTIKNLHA KIIEKDAMIK 730 740 750 760 770 780 VLQQRSRKDA GKTDSSSLRP ARSVPSIAAA TGTHSRQTSL TSSQLAEEKK EEKTWKGSIG 790 800 810 820 830 840 LLLGKEHHEH ASAPLLPPPP TSALSSIAST TAASSAHAKT GSKDSSTQTD KSAELFWPSM 850 860 870 880 890 900 ASLPSRGRLS TTPAHSPVLK HPAAKGTAEK LENSPGHGKS PDHRGRVSSL LHKPEFPDGE MMEVLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of a long cDNA clone isolated from human spleen." Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O. Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Spleen.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Skeletal muscle and Testis.
[3]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Muscle and Skin.
[5]	"Angiomotin belongs to a novel protein family with conserved coiled-coil and PDZ binding domains." Bratt A., Wilson W.J., Troyanovsky B., Aase K., Kessler R., Van Meir E.G., Holmgren L. Gene 298:69-77(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-557 (ISOFORM 1). Tissue: Brain.
[6]	Erratum Bratt A., Wilson W.J., Troyanovsky B., Aase K., Kessler R., Van Meir E.G., Holmgren L. Gene 310:231-231(2003)
[7]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[9]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-241; SER-295; SER-720 AND SER-906, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"The Nedd4-like ubiquitin E3 ligases target angiomotin/p130 to ubiquitin-dependent degradation." Wang C., An J., Zhang P., Xu C., Gao K., Wu D., Wang D., Yu H., Liu J.O., Yu L. Biochem. J. 444:279-289(2012) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION BY NEDD4.
[12]	"The Amotl2 gene inhibits Wnt/beta-catenin signaling and regulates embryonic development in zebrafish." Li Z., Wang Y., Zhang M., Xu P., Huang H., Wu D., Meng A. J. Biol. Chem. 287:13005-13015(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK074084 mRNA. Translation: BAB84910.1. Sequence problems. AL833833 mRNA. Translation: CAD38693.1. AP001152 Genomic DNA. No translation available. AP002376 Genomic DNA. No translation available. BX648729 mRNA. Translation: CAH56180.1. BC014126 mRNA. Translation: AAH14126.1. BC037539 mRNA. Translation: AAH37539.1. AF453742 mRNA. Translation: AAL49764.1.
IPI	IPI00217664. IPI00651638.
RefSeq	NP_570899.1. NM_130847.2.
UniGene	Hs.503594.
3D structure databases
ProteinModelPortal	Q8IY63.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8IY63. 4 interactions.
MINT	MINT-2809859.
STRING	9606.ENSP00000387739.
PTM databases
PhosphoSite	Q8IY63.
Polymorphism databases
DMDM	74728292.
Proteomic databases
PaxDb	Q8IY63.
PRIDE	Q8IY63.
Protocols and materials databases
DNASU	154810.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000317829; ENSP00000320968; ENSG00000166025. ENST00000433060; ENSP00000387739; ENSG00000166025.
GeneID	154810.
KEGG	hsa:154810.
UCSC	uc001pfb.3. human. uc001pfc.3. human.
Organism-specific databases
CTD	154810.
GeneCards	GC11P094441.
HGNC	HGNC:17811. AMOTL1.
HPA	HPA001196.
MIM	614657. gene.
neXtProt	NX_Q8IY63.
PharmGKB	PA24774.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG131728.
HOGENOM	HOG000233789.
HOVERGEN	HBG066485.
InParanoid	Q8IY63.
KO	K06104.
OMA	QEHGLFY.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q8IY63.
Bgee	Q8IY63.
CleanEx	HS_AMOTL1.
Genevestigator	Q8IY63.
GermOnline	ENSG00000166025. Homo sapiens.
Family and domain databases
InterPro	IPR009114. Angiomotin. IPR024646. Angiomotin_C. [Graphical view]
PANTHER	PTHR14826. PTHR14826. 1 hit.
Pfam	PF12240. Angiomotin_C. 1 hit. [Graphical view]
PRINTS	PR01807. ANGIOMOTIN.
ProtoNet	Search...
Other
ChiTaRS	AMOTL1. human.
GenomeRNAi	154810.
NextBio	87322.
SOURCE	Search...

Entry information

Entry name

AMOL1_HUMAN

Accession

Primary (citable) accession number: Q8IY63
Secondary accession number(s): Q63HK7 , Q8NDN0, Q8TEN8, Q8WXD1, Q96CM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	March 1, 2003
Last modified:	May 29, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.