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Q8IY63

- AMOL1_HUMAN

UniProt

Q8IY63 - AMOL1_HUMAN

Protein

Angiomotin-like protein 1

Gene

AMOTL1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting CTNNB1 to recycling endosomes and hence preventing its translocation to the nucleus.1 Publication

    GO - Molecular functioni

    1. identical protein binding Source: MGI
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. establishment of cell polarity involved in ameboidal cell migration Source: Ensembl
    2. hippo signaling Source: Reactome
    3. positive regulation of blood vessel endothelial cell migration Source: Ensembl
    4. Wnt signaling pathway Source: UniProtKB-KW

    Keywords - Biological processi

    Wnt signaling pathway

    Enzyme and pathway databases

    ReactomeiREACT_118607. Signaling by Hippo.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Angiomotin-like protein 1
    Gene namesi
    Name:AMOTL1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:17811. AMOTL1.

    Subcellular locationi

    Cell junctiontight junction By similarity

    GO - Cellular componenti

    1. apical plasma membrane Source: Ensembl
    2. cell junction Source: HPA
    3. cytoplasm Source: HPA
    4. cytoplasmic vesicle Source: Ensembl
    5. cytosol Source: Reactome
    6. lamellipodium Source: Ensembl
    7. tight junction Source: MGI

    Keywords - Cellular componenti

    Cell junction, Tight junction

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA24774.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 956956Angiomotin-like protein 1PRO_0000190670Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei241 – 2411Phosphoserine1 Publication
    Modified residuei295 – 2951Phosphoserine1 Publication
    Modified residuei720 – 7201Phosphoserine1 Publication
    Modified residuei906 – 9061Phosphoserine1 Publication

    Post-translational modificationi

    Polyubiquitinated by NEDD4, leading to proteasomal degradation.1 Publication

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ8IY63.
    PaxDbiQ8IY63.
    PRIDEiQ8IY63.

    PTM databases

    PhosphoSiteiQ8IY63.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8IY63.
    BgeeiQ8IY63.
    CleanExiHS_AMOTL1.
    GenevestigatoriQ8IY63.

    Organism-specific databases

    HPAiHPA001196.

    Interactioni

    Protein-protein interaction databases

    BioGridi127559. 13 interactions.
    IntActiQ8IY63. 8 interactions.
    MINTiMINT-2809859.
    STRINGi9606.ENSP00000387739.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8IY63.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili259 – 27921Sequence AnalysisAdd
    BLAST
    Coiled coili438 – 639202Sequence AnalysisAdd
    BLAST
    Coiled coili665 – 69430Sequence AnalysisAdd
    BLAST
    Coiled coili729 – 76234Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi953 – 9564PDZ-binding

    Sequence similaritiesi

    Belongs to the angiomotin family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG131728.
    HOGENOMiHOG000233789.
    HOVERGENiHBG066485.
    InParanoidiQ8IY63.
    KOiK06104.
    OMAiQEHGLFY.
    OrthoDBiEOG7M6D6Q.
    PhylomeDBiQ8IY63.
    TreeFamiTF333368.

    Family and domain databases

    InterProiIPR009114. Angiomotin.
    IPR024646. Angiomotin_C.
    [Graphical view]
    PANTHERiPTHR14826. PTHR14826. 1 hit.
    PfamiPF12240. Angiomotin_C. 1 hit.
    [Graphical view]
    PRINTSiPR01807. ANGIOMOTIN.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8IY63-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MWRAKLRRGT CEPAVKGSPS ACYSPSSPVQ VLEDSTYFSP DFQLYSGRHE    50
    TSALTVEATS SIREKVVEDP LCNFHSPNFL RISEVEMRGS EDAAAGTVLQ 100
    RLIQEQLRYG TPTENMNLLA IQHQATGSAG PAHPTNNFSS TENLTQEDPQ 150
    MVYQSARQEP QGQEHQVDNT VMEKQVRSTQ PQQNNEELPT YEEAKAQSQF 200
    FRGQQQQQQQ QGAVGHGYYM AGGTSQKSRT EGRPTVNRAN SGQAHKDEAL 250
    KELKQGHVRS LSERIMQLSL ERNGAKQHLP GSGNGKGFKV GGGPSPAQPA 300
    GKVLDPRGPP PEYPFKTKQM MSPVSKTQEH GLFYGDQHPG MLHEMVKPYP 350
    APQPVRTDVA VLRYQPPPEY GVTSRPCQLP FPSTMQQHSP MSSQTSSASG 400
    PLHSVSLPLP LPMALGAPQP PPAASPSQQL GPDAFAIVER AQQMVEILTE 450
    ENRVLHQELQ GYYDNADKLH KFEKELQRIS EAYESLVKST TKRESLDKAM 500
    RNKLEGEIRR LHDFNRDLRD RLETANRQLS SREYEGHEDK AAEGHYASQN 550
    KEFLKEKEKL EMELAAVRTA SEDHRRHIEI LDQALSNAQA RVIKLEEELR 600
    EKQAYVEKVE KLQQALTQLQ SACEKREQME RRLRTWLERE LDALRTQQKH 650
    GNGQPANMPE YNAPALLELV REKEERILAL EADMTKWEQK YLEESTIRHF 700
    AMNAAATAAA ERDTTIINHS RNGSYGESSL EAHIWQEEEE VVQANRRCQD 750
    MEYTIKNLHA KIIEKDAMIK VLQQRSRKDA GKTDSSSLRP ARSVPSIAAA 800
    TGTHSRQTSL TSSQLAEEKK EEKTWKGSIG LLLGKEHHEH ASAPLLPPPP 850
    TSALSSIAST TAASSAHAKT GSKDSSTQTD KSAELFWPSM ASLPSRGRLS 900
    TTPAHSPVLK HPAAKGTAEK LENSPGHGKS PDHRGRVSSL LHKPEFPDGE 950
    MMEVLI 956
    Length:956
    Mass (Da):106,574
    Last modified:March 1, 2003 - v1
    Checksum:i6F47AE3A13816E95
    GO
    Isoform 2 (identifier: Q8IY63-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         17-66: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:906
    Mass (Da):101,179
    Checksum:i8CFA1AE879538953
    GO

    Sequence cautioni

    The sequence BAB84910.1 differs from that shown. Reason: Frameshift at positions 592 and 944.
    The sequence BAB84910.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti459 – 4602LQ → HE in AAH14126. (PubMed:15489334)Curated
    Sequence conflicti649 – 6491K → E in CAH56180. (PubMed:17974005)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti847 – 8471P → L.
    Corresponds to variant rs11020968 [ dbSNP | Ensembl ].
    VAR_033498

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei17 – 6650Missing in isoform 2. 1 PublicationVSP_015710Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK074084 mRNA. Translation: BAB84910.1. Sequence problems.
    AL833833 mRNA. Translation: CAD38693.1.
    AP001152 Genomic DNA. No translation available.
    AP002376 Genomic DNA. No translation available.
    BX648729 mRNA. Translation: CAH56180.1.
    BC014126 mRNA. Translation: AAH14126.1.
    BC037539 mRNA. Translation: AAH37539.1.
    AF453742 mRNA. Translation: AAL49764.1.
    CCDSiCCDS44712.1. [Q8IY63-1]
    RefSeqiNP_570899.1. NM_130847.2. [Q8IY63-1]
    XP_005273857.1. XM_005273800.2. [Q8IY63-2]
    UniGeneiHs.503594.

    Genome annotation databases

    EnsembliENST00000317829; ENSP00000320968; ENSG00000166025. [Q8IY63-2]
    ENST00000433060; ENSP00000387739; ENSG00000166025. [Q8IY63-1]
    GeneIDi154810.
    KEGGihsa:154810.
    UCSCiuc001pfb.3. human. [Q8IY63-1]
    uc001pfc.3. human. [Q8IY63-2]

    Polymorphism databases

    DMDMi74728292.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK074084 mRNA. Translation: BAB84910.1 . Sequence problems.
    AL833833 mRNA. Translation: CAD38693.1 .
    AP001152 Genomic DNA. No translation available.
    AP002376 Genomic DNA. No translation available.
    BX648729 mRNA. Translation: CAH56180.1 .
    BC014126 mRNA. Translation: AAH14126.1 .
    BC037539 mRNA. Translation: AAH37539.1 .
    AF453742 mRNA. Translation: AAL49764.1 .
    CCDSi CCDS44712.1. [Q8IY63-1 ]
    RefSeqi NP_570899.1. NM_130847.2. [Q8IY63-1 ]
    XP_005273857.1. XM_005273800.2. [Q8IY63-2 ]
    UniGenei Hs.503594.

    3D structure databases

    ProteinModelPortali Q8IY63.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 127559. 13 interactions.
    IntActi Q8IY63. 8 interactions.
    MINTi MINT-2809859.
    STRINGi 9606.ENSP00000387739.

    PTM databases

    PhosphoSitei Q8IY63.

    Polymorphism databases

    DMDMi 74728292.

    Proteomic databases

    MaxQBi Q8IY63.
    PaxDbi Q8IY63.
    PRIDEi Q8IY63.

    Protocols and materials databases

    DNASUi 154810.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000317829 ; ENSP00000320968 ; ENSG00000166025 . [Q8IY63-2 ]
    ENST00000433060 ; ENSP00000387739 ; ENSG00000166025 . [Q8IY63-1 ]
    GeneIDi 154810.
    KEGGi hsa:154810.
    UCSCi uc001pfb.3. human. [Q8IY63-1 ]
    uc001pfc.3. human. [Q8IY63-2 ]

    Organism-specific databases

    CTDi 154810.
    GeneCardsi GC11P094441.
    HGNCi HGNC:17811. AMOTL1.
    HPAi HPA001196.
    MIMi 614657. gene.
    neXtProti NX_Q8IY63.
    PharmGKBi PA24774.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG131728.
    HOGENOMi HOG000233789.
    HOVERGENi HBG066485.
    InParanoidi Q8IY63.
    KOi K06104.
    OMAi QEHGLFY.
    OrthoDBi EOG7M6D6Q.
    PhylomeDBi Q8IY63.
    TreeFami TF333368.

    Enzyme and pathway databases

    Reactomei REACT_118607. Signaling by Hippo.

    Miscellaneous databases

    ChiTaRSi AMOTL1. human.
    GeneWikii AMOTL1.
    GenomeRNAii 154810.
    NextBioi 87322.
    PROi Q8IY63.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8IY63.
    Bgeei Q8IY63.
    CleanExi HS_AMOTL1.
    Genevestigatori Q8IY63.

    Family and domain databases

    InterProi IPR009114. Angiomotin.
    IPR024646. Angiomotin_C.
    [Graphical view ]
    PANTHERi PTHR14826. PTHR14826. 1 hit.
    Pfami PF12240. Angiomotin_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01807. ANGIOMOTIN.
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of a long cDNA clone isolated from human spleen."
      Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O.
      Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Spleen.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Skeletal muscle and Testis.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Muscle and Skin.
    5. "Angiomotin belongs to a novel protein family with conserved coiled-coil and PDZ binding domains."
      Bratt A., Wilson W.J., Troyanovsky B., Aase K., Kessler R., Van Meir E.G., Holmgren L.
      Gene 298:69-77(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-557 (ISOFORM 1).
      Tissue: Brain.
    6. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
      Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
      J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-241; SER-295; SER-720 AND SER-906, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "The Nedd4-like ubiquitin E3 ligases target angiomotin/p130 to ubiquitin-dependent degradation."
      Wang C., An J., Zhang P., Xu C., Gao K., Wu D., Wang D., Yu H., Liu J.O., Yu L.
      Biochem. J. 444:279-289(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION BY NEDD4.
    11. "The Amotl2 gene inhibits Wnt/beta-catenin signaling and regulates embryonic development in zebrafish."
      Li Z., Wang Y., Zhang M., Xu P., Huang H., Wu D., Meng A.
      J. Biol. Chem. 287:13005-13015(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiAMOL1_HUMAN
    AccessioniPrimary (citable) accession number: Q8IY63
    Secondary accession number(s): Q63HK7
    , Q8NDN0, Q8TEN8, Q8WXD1, Q96CM5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2005
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3