Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Intraflagellar transport protein 20 homolog

Gene

IFT20

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of intraflagellar transport (IFT) particles involved in ciliary process assembly. May play a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium. Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment.1 Publication

GO - Biological processi

  1. cardiac muscle cell differentiation Source: Ensembl
  2. centrosome localization Source: GO_Central
  3. cilium assembly Source: UniProtKB
  4. intraciliary transport Source: GO_Central
  5. kidney development Source: Ensembl
  6. neural precursor cell proliferation Source: Ensembl
  7. opsin transport Source: Ensembl
  8. organelle organization Source: Reactome
  9. photoreceptor cell outer segment organization Source: Ensembl
  10. protein localization to cilium Source: GO_Central
  11. protein localization to Golgi apparatus Source: MGI
  12. regulation of autophagic vacuole assembly Source: UniProtKB
  13. regulation of canonical Wnt signaling pathway Source: Ensembl
  14. regulation of cilium assembly Source: UniProtKB
  15. smoothened signaling pathway Source: Ensembl
  16. visual learning Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Enzyme and pathway databases

ReactomeiREACT_268024. Intraflagellar transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Intraflagellar transport protein 20 homolog
Short name:
hIFT20
Gene namesi
Name:IFT20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:30989. IFT20.

Subcellular locationi

Golgi apparatuscis-Golgi network By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole By similarity. Cytoplasmcytoskeletoncilium basal body By similarity
Note: Present at the centrosomes during the cell cycle and associated with the proximal portion of the mother centriole and the lateral aspect of the daughter centriole. Associated with basal body at the base of primary cilia (By similarity).By similarity

GO - Cellular componenti

  1. centriole Source: UniProtKB-SubCell
  2. centrosome Source: MGI
  3. ciliary basal body Source: Ensembl
  4. ciliary base Source: Ensembl
  5. ciliary tip Source: Reactome
  6. cilium Source: GO_Central
  7. dendrite terminus Source: Ensembl
  8. extracellular vesicular exosome Source: UniProtKB
  9. Golgi apparatus Source: GO_Central
  10. Golgi membrane Source: Reactome
  11. intraciliary transport particle B Source: UniProtKB
  12. microtubule cytoskeleton Source: HPA
  13. motile cilium Source: Ensembl
  14. photoreceptor connecting cilium Source: Ensembl
  15. photoreceptor outer segment Source: Ensembl
  16. primary cilium Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Golgi apparatus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671663.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 132132Intraflagellar transport protein 20 homologPRO_0000249303Add
BLAST

Proteomic databases

MaxQBiQ8IY31.
PaxDbiQ8IY31.
PRIDEiQ8IY31.

Expressioni

Tissue specificityi

Expressed in almost all tissues.1 Publication

Gene expression databases

BgeeiQ8IY31.
CleanExiHS_IFT20.
ExpressionAtlasiQ8IY31. baseline and differential.
GenevestigatoriQ8IY31.

Organism-specific databases

HPAiHPA021376.

Interactioni

Subunit structurei

Component of the IFT complex B, at least composed of IFT20, IFT22, HSPB11/IFT25, IFT27, IFT46, IFT52, TRAF3IP1/IFT54, IFT57, IFT74, IFT80, IFT81, and IFT88. Interacts directly with IFT57 and KIF3B/Kinesin II subunit. Interacts with IFT88 (By similarity). Interacts with CEP83.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
KXD1Q9BQD32EBI-744203,EBI-739657
ZDHHC17Q8IUH53EBI-9091197,EBI-524753

Protein-protein interaction databases

BioGridi124711. 31 interactions.
IntActiQ8IY31. 14 interactions.
MINTiMINT-1193441.
STRINGi9606.ENSP00000350570.

Structurei

3D structure databases

ProteinModelPortaliQ8IY31.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni70 – 13263IFT57-bindingBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili74 – 11441Sequence AnalysisAdd
BLAST

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG41943.
GeneTreeiENSGT00390000003413.
HOGENOMiHOG000230527.
HOVERGENiHBG081781.
InParanoidiQ8IY31.
KOiK16473.
OMAiFIDQFIL.
PhylomeDBiQ8IY31.
TreeFamiTF319434.

Family and domain databases

InterProiIPR028172. FT20.
[Graphical view]
PfamiPF14931. IFT20. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8IY31-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKDILGEAG LHFDELNKLR VLDPEVTQQT IELKEECKDF VDKIGQFQKI
60 70 80 90 100
VGGLIELVDQ LAKEAENEKM KAIGARNLLK SIAKQREAQQ QQLQALIAEK
110 120 130
KMQLERYRVE YEALCKVEAE QNEFIDQFIF QK
Length:132
Mass (Da):15,281
Last modified:February 28, 2003 - v1
Checksum:i8C751DE18FEEB30F
GO
Isoform 2 (identifier: Q8IY31-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MTHLLLTATVTPSEQNSSREPGWETAM

Show »
Length:158
Mass (Da):18,120
Checksum:i8CB5C24AC62F22D1
GO
Isoform 3 (identifier: Q8IY31-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     72-132: AIGARNLLKS...EFIDQFIFQK → SLAVSPRLEC...KENAARKVSG

Show »
Length:148
Mass (Da):15,964
Checksum:iC19CC3F33BC56CA8
GO
Isoform 4 (identifier: Q8IY31-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     107-132: YRVEYEALCKVEAEQNEFIDQFIFQK → NELKISLL

Note: No experimental confirmation available.

Show »
Length:114
Mass (Da):12,969
Checksum:i80A74AE1A98303BA
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MTHLLLTATVTPSEQNSSRE PGWETAM in isoform 2. 1 PublicationVSP_020390
Alternative sequencei72 – 13261AIGAR…FIFQK → SLAVSPRLECTGAISAHCKL CLSDSSDSPTSPSRVGGTTG HRCSELAQIYSKAERSSTAA TSSPNSRKENAARKVSG in isoform 3. 1 PublicationVSP_020391Add
BLAST
Alternative sequencei107 – 13226YRVEY…FIFQK → NELKISLL in isoform 4. 1 PublicationVSP_046026Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY224601 mRNA. Translation: AAP50265.1.
BQ639919 mRNA. No translation available.
AC002094 Genomic DNA. No translation available.
BC002640 mRNA. Translation: AAH02640.1.
BC038094 mRNA. Translation: AAH38094.1.
CCDSiCCDS32593.1. [Q8IY31-3]
CCDS58533.1. [Q8IY31-4]
CCDS58534.1. [Q8IY31-1]
CCDS58535.1. [Q8IY31-2]
RefSeqiNP_001254703.1. NM_001267774.1. [Q8IY31-2]
NP_001254704.1. NM_001267775.1. [Q8IY31-1]
NP_001254705.1. NM_001267776.1. [Q8IY31-1]
NP_001254706.1. NM_001267777.1. [Q8IY31-4]
NP_001254707.1. NM_001267778.1.
NP_777547.1. NM_174887.3. [Q8IY31-3]
UniGeneiHs.705431.
Hs.744876.

Genome annotation databases

EnsembliENST00000357896; ENSP00000350570; ENSG00000109083. [Q8IY31-3]
ENST00000395418; ENSP00000378809; ENSG00000109083. [Q8IY31-1]
ENST00000579419; ENSP00000463322; ENSG00000109083. [Q8IY31-4]
ENST00000585089; ENSP00000464443; ENSG00000109083. [Q8IY31-2]
ENST00000585313; ENSP00000463138; ENSG00000109083. [Q8IY31-1]
GeneIDi90410.
KEGGihsa:90410.
UCSCiuc002hau.2. human. [Q8IY31-3]
uc002hav.2. human. [Q8IY31-2]
uc002haw.2. human. [Q8IY31-1]

Polymorphism databases

DMDMi74728279.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY224601 mRNA. Translation: AAP50265.1.
BQ639919 mRNA. No translation available.
AC002094 Genomic DNA. No translation available.
BC002640 mRNA. Translation: AAH02640.1.
BC038094 mRNA. Translation: AAH38094.1.
CCDSiCCDS32593.1. [Q8IY31-3]
CCDS58533.1. [Q8IY31-4]
CCDS58534.1. [Q8IY31-1]
CCDS58535.1. [Q8IY31-2]
RefSeqiNP_001254703.1. NM_001267774.1. [Q8IY31-2]
NP_001254704.1. NM_001267775.1. [Q8IY31-1]
NP_001254705.1. NM_001267776.1. [Q8IY31-1]
NP_001254706.1. NM_001267777.1. [Q8IY31-4]
NP_001254707.1. NM_001267778.1.
NP_777547.1. NM_174887.3. [Q8IY31-3]
UniGeneiHs.705431.
Hs.744876.

3D structure databases

ProteinModelPortaliQ8IY31.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124711. 31 interactions.
IntActiQ8IY31. 14 interactions.
MINTiMINT-1193441.
STRINGi9606.ENSP00000350570.

Polymorphism databases

DMDMi74728279.

Proteomic databases

MaxQBiQ8IY31.
PaxDbiQ8IY31.
PRIDEiQ8IY31.

Protocols and materials databases

DNASUi90410.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000357896; ENSP00000350570; ENSG00000109083. [Q8IY31-3]
ENST00000395418; ENSP00000378809; ENSG00000109083. [Q8IY31-1]
ENST00000579419; ENSP00000463322; ENSG00000109083. [Q8IY31-4]
ENST00000585089; ENSP00000464443; ENSG00000109083. [Q8IY31-2]
ENST00000585313; ENSP00000463138; ENSG00000109083. [Q8IY31-1]
GeneIDi90410.
KEGGihsa:90410.
UCSCiuc002hau.2. human. [Q8IY31-3]
uc002hav.2. human. [Q8IY31-2]
uc002haw.2. human. [Q8IY31-1]

Organism-specific databases

CTDi90410.
GeneCardsiGC17M026655.
HGNCiHGNC:30989. IFT20.
HPAiHPA021376.
MIMi614394. gene.
neXtProtiNX_Q8IY31.
PharmGKBiPA142671663.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG41943.
GeneTreeiENSGT00390000003413.
HOGENOMiHOG000230527.
HOVERGENiHBG081781.
InParanoidiQ8IY31.
KOiK16473.
OMAiFIDQFIL.
PhylomeDBiQ8IY31.
TreeFamiTF319434.

Enzyme and pathway databases

ReactomeiREACT_268024. Intraflagellar transport.

Miscellaneous databases

ChiTaRSiIFT20. human.
GeneWikiiIFT20.
GenomeRNAii90410.
NextBioi35536454.
PROiQ8IY31.
SOURCEiSearch...

Gene expression databases

BgeeiQ8IY31.
CleanExiHS_IFT20.
ExpressionAtlasiQ8IY31. baseline and differential.
GenevestigatoriQ8IY31.

Family and domain databases

InterProiIPR028172. FT20.
[Graphical view]
PfamiPF14931. IFT20. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the human IFT20 gene."
    Yin G., Dai J., Ji C., Ni X., Shu G., Ye X., Dai J., Wu Q., Gu S., Xie Y., Zhao R.C., Mao Y.
    Mol. Biol. Rep. 30:255-260(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
  2. "Expressed sequence tag analysis of human retina for the NEIBank project: retbindin, an abundant, novel retinal cDNA and alternative splicing of other retina-preferred gene transcripts."
    Wistow G., Berstein S.L., Wyatt M.K., Ray S., Behal A., Touchman J.W., Bouffard G., Smith D., Peterson K.
    Mol. Vis. 8:196-204(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
  3. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Tissue: Uterus.
  5. "The intraflagellar transport protein IFT20 is associated with the Golgi complex and is required for cilia assembly."
    Follit J.A., Tuft R.A., Fogarty K.E., Pazour G.J.
    Mol. Biol. Cell 17:3781-3792(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: INTERACTION WITH CEP83.

Entry informationi

Entry nameiIFT20_HUMAN
AccessioniPrimary (citable) accession number: Q8IY31
Secondary accession number(s): J3QL09, Q5GLZ2, Q9BUG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 4, 2006
Last sequence update: February 28, 2003
Last modified: March 31, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.