Q8IY31 (IFT20_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Intraflagellar transport protein 20 homolog
Short name=hIFT20

Gene names

Name:

IFT20

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	132 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of intraflagellar transport (IFT) particles involved in ciliary process assembly. May play a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium. Ref.5
Subunit structure	Component of IFT complex B composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. Interacts directly with IFT57 and KIF3B/Kinesin II subunit By similarity.
Subcellular location	Golgi apparatus › cis-Golgi network By similarity. Cytoplasm › cytoskeleton › centrosome › centriole By similarity. Cytoplasm › cytoskeleton › cilium basal body By similarity. Note: Present at the centrosomes during the cell cycle and associated with the proximal portion of the mother centriole and the lateral aspect of the daughter centriole. Associated with basal body at the base of primary cilia By similarity.
Tissue specificity	Expressed in almost all tissues. Ref.1

Ontologies

Keywords
Biological process	Cilium biogenesis/degradation
Cellular component	Cell projection Cilium Cytoplasm Cytoskeleton Golgi apparatus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell projection organization Inferred from electronic annotation. Source: UniProtKB-KW centrosome localization Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell centriole Inferred from electronic annotation. Source: UniProtKB-SubCell cilium Inferred from electronic annotation. Source: UniProtKB-KW dendrite terminus Inferred from electronic annotation. Source: Compara intraflagellar transport particle B Inferred from electronic annotation. Source: Compara microtubule basal body Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
KXD1	Q9BQD3	2	EBI-744203,EBI-739657

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8IY31-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8IY31-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MTHLLLTATVTPSEQNSSREPGWETAM

Isoform 3 (identifier: Q8IY31-3)

The sequence of this isoform differs from the canonical sequence as follows:
     72-132: AIGARNLLKS...EFIDQFIFQK → SLAVSPRLEC...KENAARKVSG

Isoform 4 (identifier: Q8IY31-4)

The sequence of this isoform differs from the canonical sequence as follows:
     107-132: YRVEYEALCKVEAEQNEFIDQFIFQK → NELKISLL

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 132	132	Intraflagellar transport protein 20 homolog		PRO_0000249303
Regions
	Region	70 – 132	63	IFT57-binding By similarity
	Coiled coil	74 – 114	41	Potential
Natural variations
	Alternative sequence	1	1	M → MTHLLLTATVTPSEQNSSRE PGWETAM in isoform 2.		VSP_020390
	Alternative sequence	72 – 132	61	AIGAR…FIFQK → SLAVSPRLECTGAISAHCKL CLSDSSDSPTSPSRVGGTTG HRCSELAQIYSKAERSSTAA TSSPNSRKENAARKVSG in isoform 3.		VSP_020391
	Alternative sequence	107 – 132	26	YRVEY…FIFQK → NELKISLL in isoform 4.		VSP_046026

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 8C751DE18FEEB30F	FASTA	132	15,281
	10 20 30 40 50 60 MAKDILGEAG LHFDELNKLR VLDPEVTQQT IELKEECKDF VDKIGQFQKI VGGLIELVDQ 70 80 90 100 110 120 LAKEAENEKM KAIGARNLLK SIAKQREAQQ QQLQALIAEK KMQLERYRVE YEALCKVEAE 130 QNEFIDQFIF QK
	Isoform 2 [UniParc]. Checksum: 8CB5C24AC62F22D1	FASTA	158	18,120
	10 20 30 40 50 60 MTHLLLTATV TPSEQNSSRE PGWETAMAKD ILGEAGLHFD ELNKLRVLDP EVTQQTIELK 70 80 90 100 110 120 EECKDFVDKI GQFQKIVGGL IELVDQLAKE AENEKMKAIG ARNLLKSIAK QREAQQQQLQ 130 140 150 ALIAEKKMQL ERYRVEYEAL CKVEAEQNEF IDQFIFQK
	Isoform 3 [UniParc]. Checksum: C19CC3F33BC56CA8	FASTA	148	15,964
	10 20 30 40 50 60 MAKDILGEAG LHFDELNKLR VLDPEVTQQT IELKEECKDF VDKIGQFQKI VGGLIELVDQ 70 80 90 100 110 120 LAKEAENEKM KSLAVSPRLE CTGAISAHCK LCLSDSSDSP TSPSRVGGTT GHRCSELAQI 130 140 YSKAERSSTA ATSSPNSRKE NAARKVSG
	Isoform 4 [UniParc]. Checksum: 80A74AE1A98303BA	FASTA	114	12,969
	10 20 30 40 50 60 MAKDILGEAG LHFDELNKLR VLDPEVTQQT IELKEECKDF VDKIGQFQKI VGGLIELVDQ 70 80 90 100 110 LAKEAENEKM KAIGARNLLK SIAKQREAQQ QQLQALIAEK KMQLERNELK ISLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the human IFT20 gene." Yin G., Dai J., Ji C., Ni X., Shu G., Ye X., Dai J., Wu Q., Gu S., Xie Y., Zhao R.C., Mao Y. Mol. Biol. Rep. 30:255-260(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
[2]	"Expressed sequence tag analysis of human retina for the NEIBank project: retbindin, an abundant, novel retinal cDNA and alternative splicing of other retina-preferred gene transcripts." Wistow G., Berstein S.L., Wyatt M.K., Ray S., Behal A., Touchman J.W., Bouffard G., Smith D., Peterson K. Mol. Vis. 8:196-204(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
[3]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Uterus.
[5]	"The intraflagellar transport protein IFT20 is associated with the Golgi complex and is required for cilia assembly." Follit J.A., Tuft R.A., Fogarty K.E., Pazour G.J. Mol. Biol. Cell 17:3781-3792(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY224601 mRNA. Translation: AAP50265.1. BQ639919 mRNA. No translation available. AC002094 Genomic DNA. No translation available. BC002640 mRNA. Translation: AAH02640.1. BC038094 mRNA. Translation: AAH38094.1.
IPI	IPI00217619. IPI00414091. IPI00784847.
RefSeq	NP_001254703.1. NM_001267774.1. NP_001254704.1. NM_001267775.1. NP_001254705.1. NM_001267776.1. NP_001254706.1. NM_001267777.1. NP_001254707.1. NM_001267778.1. NP_777547.1. NM_174887.3.
UniGene	Hs.705431. Hs.741252.
3D structure databases
ProteinModelPortal	Q8IY31.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8IY31. 11 interactions.
MINT	MINT-1193441.
STRING	9606.ENSP00000350570.
Polymorphism databases
DMDM	74728279.
Proteomic databases
PaxDb	Q8IY31.
PRIDE	Q8IY31.
Protocols and materials databases
DNASU	90410.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000357896; ENSP00000350570; ENSG00000109083. ENST00000395418; ENSP00000378809; ENSG00000109083. ENST00000573233; ENSP00000461706; ENSG00000262866. ENST00000575742; ENSP00000458458; ENSG00000262866. ENST00000577872; ENSP00000462871; ENSG00000262866. ENST00000579419; ENSP00000463322; ENSG00000109083. ENST00000580505; ENSP00000463828; ENSG00000262866. ENST00000580696; ENSP00000463655; ENSG00000262866. ENST00000585089; ENSP00000464443; ENSG00000109083. ENST00000585313; ENSP00000463138; ENSG00000109083.
GeneID	90410.
KEGG	hsa:90410.
UCSC	uc002hau.1. human. uc002hav.1. human. uc002haw.1. human.
Organism-specific databases
CTD	90410.
GeneCards	GC17M026655.
HGNC	HGNC:30989. IFT20.
HPA	HPA021376.
MIM	614394. gene.
neXtProt	NX_Q8IY31.
PharmGKB	PA142671663.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG41943.
HOGENOM	HOG000230527.
HOVERGEN	HBG081781.
InParanoid	Q8IY31.
KO	K16473.
OMA	MKAIGSR.
OrthoDB	EOG4JDH89.
Gene expression databases
Bgee	Q8IY31.
CleanEx	HS_IFT20.
Genevestigator	Q8IY31.
GermOnline	ENSG00000109083. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other
ChiTaRS	IFT20. human.
GenomeRNAi	90410.
NextBio	35536454.
SOURCE	Search...

Entry information

Entry name

IFT20_HUMAN

Accession

Primary (citable) accession number: Q8IY31
Secondary accession number(s): J3QL09, Q5GLZ2, Q9BUG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.