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Protein

Probable ATP-dependent RNA helicase DDX60

Gene

DDX60

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Positively regulates DDX58/RIG-I- and IFIH1/MDA5-dependent type I interferon and interferon inducible gene expression in response to viral infection. Binds ssRNA, dsRNA and dsDNA and can promote the binding of DDX58/RIG-I to dsRNA. Exhibits antiviral activity against hepatitis C virus and vesicular stomatitis virus (VSV).2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi785 – 792ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • double-stranded DNA binding Source: UniProtKB
  • double-stranded RNA binding Source: UniProtKB
  • helicase activity Source: UniProtKB-KW
  • single-stranded RNA binding Source: UniProtKB

GO - Biological processi

  • defense response to virus Source: UniProtKB-KW
  • innate immune response Source: UniProtKB-KW
  • positive regulation of MDA-5 signaling pathway Source: UniProtKB
  • positive regulation of RIG-I signaling pathway Source: UniProtKB
  • response to virus Source: UniProtKB

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
Biological processAntiviral defense, Immunity, Innate immunity
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX60 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 60
Gene namesi
Name:DDX60
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000137628.16
HGNCiHGNC:25942 DDX60
MIMi613974 gene
neXtProtiNX_Q8IY21

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi55601
OpenTargetsiENSG00000137628
PharmGKBiPA162383444

Polymorphism and mutation databases

BioMutaiDDX60
DMDMi296439377

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003181541 – 1712Probable ATP-dependent RNA helicase DDX60Add BLAST1712

Proteomic databases

EPDiQ8IY21
MaxQBiQ8IY21
PaxDbiQ8IY21
PeptideAtlasiQ8IY21
PRIDEiQ8IY21
ProteomicsDBi71088

PTM databases

iPTMnetiQ8IY21
PhosphoSitePlusiQ8IY21

Expressioni

Tissue specificityi

Brain, lymph node, prostate, stomach, thyroid, tongue, trachea, uterus, skeletal muscle, spleen, kidney, liver and small intestine.1 Publication

Inductioni

By interferon (IFN). Up-regulated during vesicular stomatitis virus (VSV), or poliovirus (PV) infection.2 Publications

Gene expression databases

BgeeiENSG00000137628
CleanExiHS_DDX60
ExpressionAtlasiQ8IY21 baseline and differential
GenevisibleiQ8IY21 HS

Organism-specific databases

HPAiHPA046952

Interactioni

Subunit structurei

Interacts with EXOSC1, EXOSC4, DDX58/RIG-I, IFIH1/MDA5 and DHX58/LGP2.1 Publication

Protein-protein interaction databases

BioGridi120742, 11 interactors
IntActiQ8IY21, 6 interactors
STRINGi9606.ENSP00000377344

Structurei

3D structure databases

ProteinModelPortaliQ8IY21
SMRiQ8IY21
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini772 – 939Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST168
Domaini1226 – 1370Helicase C-terminalPROSITE-ProRule annotationAdd BLAST145

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi889 – 892DEVH box4

Sequence similaritiesi

Belongs to the helicase family.Curated

Phylogenomic databases

eggNOGiKOG0949 Eukaryota
KOG0950 Eukaryota
COG4581 LUCA
GeneTreeiENSGT00820000127042
HOGENOMiHOG000169263
HOVERGENiHBG107797
InParanoidiQ8IY21
KOiK20103
OMAiFAQNSVY
OrthoDBiEOG091G02TT
PhylomeDBiQ8IY21
TreeFamiTF314846

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

Q8IY21-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERNVLTTFS QEMSQLILNE MPKAEYSSLF NDFVESEFFL IDGDSLLITC
60 70 80 90 100
ICEISFKPGQ NLHFFYLVER YLVDLISKGG QFTIVFFKDA EYAYFNFPEL
110 120 130 140 150
LSLRTALILH LQKNTTIDVR TTFSRCLSKE WGSFLEESYP YFLIVADEGL
160 170 180 190 200
NDLQTQLFNF LIIHSWARKV NVVLSSGQES DVLCLYAYLL PSMYRHQIFS
210 220 230 240 250
WKNKQNIKDA YTTLLNQLER FKLSALAPLF GSLKWNNITE EAHKTVSLLT
260 270 280 290 300
QVWPEGSDIR RVFCVTSCSL SLRMYHRFLG NREPSSGQET EIQQVNSNCL
310 320 330 340 350
TLQEMEDLCK LHCLTVVFLL HLPLSQRACA RVITSHWAED MKPLLQMKKW
360 370 380 390 400
CEYFILRNIH TFEFWNLNLI HLSDLNDELL LKNIAFYYEN ENVKGLHLNL
410 420 430 440 450
GDTIMKDYEY LWNTVSKLVR DFEVGQPFPL RTTKVCFLEK KPSPIKDSSN
460 470 480 490 500
EMVPNLGFIP TSSFVVDKFA GDILKDLPFL KSDDPIVTSL VKQKEFDELV
510 520 530 540 550
HWHSHKPLSD DYDRSRCQFD EKSRDPRVLR SVQKYHVFQR FYGNSLETVS
560 570 580 590 600
SKIIVTQTIK SKKDFSGPKS KKAHETKAEI IARENKKRLF AREEQKEEQK
610 620 630 640 650
WNALSFSIEE QLKENLHSGI KSLEDFLKSC KSSCVKLQVE MVGLTACLKA
660 670 680 690 700
WKEHCRSEEG KTTKDLSIAV QVMKRIHSLM EKYSELLQED DRQLIARCLK
710 720 730 740 750
YLGFDELASS LHPAQDAEND VKVKKRNKYS VGIGPARFQL QYMGHYLIRD
760 770 780 790 800
ERKDPDPRVQ DFIPDTWQRE LLDVVDKNES AVIVAPTSSG KTYASYYCME
810 820 830 840 850
KVLKESDDGV VVYVAPTKAL VNQVAATVQN RFTKNLPSGE VLCGVFTREY
860 870 880 890 900
RHDALNCQVL ITVPACFEIL LLAPHRQNWV KKIRYVIFDE VHCLGGEIGA
910 920 930 940 950
EIWEHLLVMI RCPFLALSAT ISNPEHLTEW LQSVKWYWKQ EDKIIENNTA
960 970 980 990 1000
SKRHVGRQAG FPKDYLQVKQ SYKVRLVLYG ERYNDLEKHV CSIKHGDIHF
1010 1020 1030 1040 1050
DHFHPCAALT TDHIERYGFP PDLTLSPRES IQLYDAMFQI WKSWPRAQEL
1060 1070 1080 1090 1100
CPENFIHFNN KLVIKKMDAR KYEESLKAEL TSWIKNGNVE QARMVLQNLS
1110 1120 1130 1140 1150
PEADLSPENM ITMFPLLVEK LRKMEKLPAL FFLFKLGAVE NAAESVSTFL
1160 1170 1180 1190 1200
KKKQETKRPP KADKEAHVMA NKLRKVKKSI EKQKIIDEKS QKKTRNVDQS
1210 1220 1230 1240 1250
LIHEAEHDNL VKCLEKNLEI PQDCTYADQK AVDTETLQKV FGRVKFERKG
1260 1270 1280 1290 1300
EELKALAERG IGYHHSAMSF KEKQLVEILF RKGYLRVVTA TGTLALGVNM
1310 1320 1330 1340 1350
PCKSVVFAQN SVYLDALNYR QMSGRAGRRG QDLMGDVYFF DIPFPKIGKL
1360 1370 1380 1390 1400
IKSNVPELRG HFPLSITLVL RLMLLASKGD DPEDAKAKVL SVLKHSLLSF
1410 1420 1430 1440 1450
KQPRVMDMLK LYFLFSLQFL VKEGYLDQEG NPMGFAGLVS HLHYHEPSNL
1460 1470 1480 1490 1500
VFVSFLVNGL FHDLCQPTRK GSKHFSQDVM EKLVLVLAHL FGRRYFPPKF
1510 1520 1530 1540 1550
QDAHFEFYQS KVFLDDLPED FSDALDEYNM KIMEDFTTFL RIVSKLADMN
1560 1570 1580 1590 1600
QEYQLPLSKI KFTGKECEDS QLVSHLMSCK EGRVAISPFV CLSGNFDDDL
1610 1620 1630 1640 1650
LRLETPNHVT LGTIGVNRSQ APVLLSQKFD NRGRKMSLNA YALDFYKHGS
1660 1670 1680 1690 1700
LIGLVQDNRM NEGDAYYLLK DFALTIKSIS VSLRELCENE DDNVVLAFEQ
1710
LSTTFWEKLN KV
Length:1,712
Mass (Da):197,853
Last modified:May 18, 2010 - v3
Checksum:i4F71D8E5A06D3EB9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti415V → I in BAA91809 (PubMed:14702039).Curated1
Sequence conflicti439E → G in AAH38115 (PubMed:15489334).Curated1
Sequence conflicti731V → I in AAH38115 (PubMed:15489334).Curated1
Sequence conflicti1385A → T in AAH38115 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_055895672V → M. Corresponds to variant dbSNP:rs550625Ensembl.1
Natural variantiVAR_055896998I → V. Corresponds to variant dbSNP:rs576619Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC068989 Genomic DNA No translation available.
BC007820 mRNA Translation: AAH07820.1
BC038115 mRNA Translation: AAH38115.1
AK001649 mRNA Translation: BAA91809.1
CCDSiCCDS34097.1
RefSeqiNP_060101.3, NM_017631.5
UniGeneiHs.591710

Genome annotation databases

EnsembliENST00000393743; ENSP00000377344; ENSG00000137628
GeneIDi55601
KEGGihsa:55601
UCSCiuc003irp.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiDDX60_HUMAN
AccessioniPrimary (citable) accession number: Q8IY21
Secondary accession number(s): Q6PK35, Q9NVE3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 18, 2010
Last modified: June 20, 2018
This is version 130 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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