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Q8IXZ3

- SP8_HUMAN

UniProt

Q8IXZ3 - SP8_HUMAN

Protein

Transcription factor Sp8

Gene

SP8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 3 (13 Jul 2010)
      Previous versions | rss
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    Functioni

    Transcription factor which plays a key role in limb development. Positively regulates FGF8 expression in the apical ectodermal ridge (AER) and contributes to limb outgrowth in embryos By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri314 – 33825C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri344 – 36825C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri374 – 39623C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. dorsal/ventral pattern formation Source: Ensembl
    2. embryonic limb morphogenesis Source: Ensembl
    3. proximal/distal pattern formation Source: Ensembl
    4. regulation of transcription, DNA-templated Source: UniProtKB-KW
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor Sp8
    Alternative name(s):
    Specificity protein 8
    Gene namesi
    Name:SP8
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:19196. SP8.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134893390.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 490490Transcription factor Sp8PRO_0000047152Add
    BLAST

    Proteomic databases

    PaxDbiQ8IXZ3.
    PRIDEiQ8IXZ3.

    PTM databases

    PhosphoSiteiQ8IXZ3.

    Expressioni

    Gene expression databases

    BgeeiQ8IXZ3.
    CleanExiHS_SP8.
    GenevestigatoriQ8IXZ3.

    Organism-specific databases

    HPAiHPA054006.

    Interactioni

    Protein-protein interaction databases

    BioGridi128760. 3 interactions.
    STRINGi9606.ENSP00000408792.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8IXZ3.
    SMRiQ8IXZ3. Positions 324-470.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi11 – 114104Ser-richAdd
    BLAST
    Compositional biasi68 – 8114Ala-richAdd
    BLAST

    Sequence similaritiesi

    Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri314 – 33825C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri344 – 36825C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri374 – 39623C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    HOGENOMiHOG000231067.
    HOVERGENiHBG036899.
    InParanoidiQ8IXZ3.
    KOiK09198.
    OMAiSASCNVV.
    OrthoDBiEOG7JQBMX.
    PhylomeDBiQ8IXZ3.
    TreeFamiTF350150.

    Family and domain databases

    Gene3Di3.30.160.60. 3 hits.
    InterProiIPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 3 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
    PS50157. ZINC_FINGER_C2H2_2. 3 hits.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 3 (identifier: Q8IXZ3-3) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLAATCNKIG SPSPSPSSLS DSSSSFGKGF HPWKRSSSSS SASCNVVGSS    50
    LSSFGVSGAS RNGGSSSAAA AAAAAAAAAA ALVSDSFSCG GSPGSSAFSL 100
    TSSSAAAAAA AAAAAASSSP FANDYSVFQA PGVSGGSGGG GGGGGGGSSA 150
    HSQDGSHQPV FISKVHTSVD GLQGIYPRVG MAHPYESWFK PSHPGLGAAG 200
    EVGSAGASSW WDVGAGWIDV QNPNSAAALP GSLHPAAGGL QTSLHSPLGG 250
    YNSDYSGLSH SAFSSGASSH LLSPAGQHLM DGFKPVLPGS YPDSAPSPLA 300
    GAGGSMLSAG PSAPLGGSPR SSARRYSGRA TCDCPNCQEA ERLGPAGASL 350
    RRKGLHSCHI PGCGKVYGKT SHLKAHLRWH TGERPFVCNW LFCGKRFTRS 400
    DELQRHLRTH TGEKRFACPV CNKRFMRSDH LSKHVKTHSG GGGGGGSAGS 450
    GSGGKKGSDT DSEHSAAGSP PCHSPELLQP PEPGHRNGLE 490
    Length:490
    Mass (Da):48,674
    Last modified:July 13, 2010 - v3
    Checksum:i8FB659057C6F8E58
    GO
    Isoform 1 (identifier: Q8IXZ3-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         104-145: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:448
    Mass (Da):45,235
    Checksum:i23D3E1CE4DBBE0AE
    GO
    Isoform 2 (identifier: Q8IXZ3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         92-147: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:434
    Mass (Da):44,012
    Checksum:i8D1AC12A65A89605
    GO
    Isoform 4 (identifier: Q8IXZ3-4) [UniParc]FASTAAdd to Basket

    Also known as: Sp8L

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MATSLLGEEPRLGSTPLAM

    Show »
    Length:508
    Mass (Da):50,500
    Checksum:i99C8728AA08D139F
    GO

    Sequence cautioni

    The sequence BAB71297.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti430 – 4301H → R in BC038669. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 11M → MATSLLGEEPRLGSTPLAM in isoform 4. 1 PublicationVSP_044094
    Alternative sequencei92 – 14756Missing in isoform 2. 1 PublicationVSP_007441Add
    BLAST
    Alternative sequencei104 – 14542Missing in isoform 1. 1 PublicationVSP_011036Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY167047 mRNA. Translation: AAO38028.1.
    AY167048 mRNA. Translation: AAO38029.1.
    AK056857 mRNA. Translation: BAB71297.1. Different initiation.
    CH471073 Genomic DNA. Translation: EAW93731.1.
    BC038669 mRNA. No translation available.
    CCDSiCCDS43555.1. [Q8IXZ3-4]
    CCDS5372.1. [Q8IXZ3-3]
    RefSeqiNP_874359.2. NM_182700.5. [Q8IXZ3-4]
    NP_945194.1. NM_198956.3. [Q8IXZ3-3]
    UniGeneiHs.195922.

    Genome annotation databases

    EnsembliENST00000361443; ENSP00000354482; ENSG00000164651. [Q8IXZ3-3]
    ENST00000418710; ENSP00000408792; ENSG00000164651. [Q8IXZ3-4]
    GeneIDi221833.
    KEGGihsa:221833.
    UCSCiuc003suy.3. human. [Q8IXZ3-3]
    uc003suz.3. human. [Q8IXZ3-4]

    Polymorphism databases

    DMDMi300669678.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY167047 mRNA. Translation: AAO38028.1 .
    AY167048 mRNA. Translation: AAO38029.1 .
    AK056857 mRNA. Translation: BAB71297.1 . Different initiation.
    CH471073 Genomic DNA. Translation: EAW93731.1 .
    BC038669 mRNA. No translation available.
    CCDSi CCDS43555.1. [Q8IXZ3-4 ]
    CCDS5372.1. [Q8IXZ3-3 ]
    RefSeqi NP_874359.2. NM_182700.5. [Q8IXZ3-4 ]
    NP_945194.1. NM_198956.3. [Q8IXZ3-3 ]
    UniGenei Hs.195922.

    3D structure databases

    ProteinModelPortali Q8IXZ3.
    SMRi Q8IXZ3. Positions 324-470.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 128760. 3 interactions.
    STRINGi 9606.ENSP00000408792.

    PTM databases

    PhosphoSitei Q8IXZ3.

    Polymorphism databases

    DMDMi 300669678.

    Proteomic databases

    PaxDbi Q8IXZ3.
    PRIDEi Q8IXZ3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000361443 ; ENSP00000354482 ; ENSG00000164651 . [Q8IXZ3-3 ]
    ENST00000418710 ; ENSP00000408792 ; ENSG00000164651 . [Q8IXZ3-4 ]
    GeneIDi 221833.
    KEGGi hsa:221833.
    UCSCi uc003suy.3. human. [Q8IXZ3-3 ]
    uc003suz.3. human. [Q8IXZ3-4 ]

    Organism-specific databases

    CTDi 221833.
    GeneCardsi GC07M020788.
    HGNCi HGNC:19196. SP8.
    HPAi HPA054006.
    MIMi 608306. gene.
    neXtProti NX_Q8IXZ3.
    PharmGKBi PA134893390.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5048.
    HOGENOMi HOG000231067.
    HOVERGENi HBG036899.
    InParanoidi Q8IXZ3.
    KOi K09198.
    OMAi SASCNVV.
    OrthoDBi EOG7JQBMX.
    PhylomeDBi Q8IXZ3.
    TreeFami TF350150.

    Miscellaneous databases

    GeneWikii Sp8_transcription_factor.
    GenomeRNAii 221833.
    NextBioi 91473.
    PROi Q8IXZ3.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8IXZ3.
    CleanExi HS_SP8.
    Genevestigatori Q8IXZ3.

    Family and domain databases

    Gene3Di 3.30.160.60. 3 hits.
    InterProi IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 3 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 3 hits.
    PS50157. ZINC_FINGER_C2H2_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic structure and cloning of two transcript isoforms of human Sp8."
      Milona M.-A., Gough J.E., Edgar A.J.
      BMC Genomics 5:86-86(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4), ALTERNATIVE SPLICING.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Prostate.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.

    Entry informationi

    Entry nameiSP8_HUMAN
    AccessioniPrimary (citable) accession number: Q8IXZ3
    Secondary accession number(s): Q7Z615, Q7Z616, Q96MJ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2003
    Last sequence update: July 13, 2010
    Last modified: October 1, 2014
    This is version 100 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3