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Protein

Transcription factor Sp8

Gene

SP8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor which plays a key role in limb development. Positively regulates FGF8 expression in the apical ectodermal ridge (AER) and contributes to limb outgrowth in embryos (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri314 – 33825C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri344 – 36825C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri374 – 39623C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. dorsal/ventral pattern formation Source: Ensembl
  2. embryonic limb morphogenesis Source: Ensembl
  3. proximal/distal pattern formation Source: Ensembl
  4. regulation of transcription, DNA-templated Source: UniProtKB-KW
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor Sp8
Alternative name(s):
Specificity protein 8
Gene namesi
Name:SP8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:19196. SP8.

Subcellular locationi

  1. Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134893390.

Polymorphism and mutation databases

BioMutaiSP8.
DMDMi300669678.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Transcription factor Sp8PRO_0000047152Add
BLAST

Proteomic databases

PaxDbiQ8IXZ3.
PRIDEiQ8IXZ3.

PTM databases

PhosphoSiteiQ8IXZ3.

Expressioni

Gene expression databases

BgeeiQ8IXZ3.
CleanExiHS_SP8.
GenevestigatoriQ8IXZ3.

Organism-specific databases

HPAiHPA054006.

Interactioni

Protein-protein interaction databases

BioGridi128760. 3 interactions.
STRINGi9606.ENSP00000408792.

Structurei

3D structure databases

ProteinModelPortaliQ8IXZ3.
SMRiQ8IXZ3. Positions 324-447.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi11 – 114104Ser-richAdd
BLAST
Compositional biasi68 – 8114Ala-richAdd
BLAST

Sequence similaritiesi

Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri314 – 33825C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri344 – 36825C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri374 – 39623C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000118984.
HOGENOMiHOG000231067.
HOVERGENiHBG036899.
InParanoidiQ8IXZ3.
KOiK09198.
OMAiFGNDYSV.
OrthoDBiEOG7JQBMX.
PhylomeDBiQ8IXZ3.
TreeFamiTF350150.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 3 (identifier: Q8IXZ3-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLAATCNKIG SPSPSPSSLS DSSSSFGKGF HPWKRSSSSS SASCNVVGSS
60 70 80 90 100
LSSFGVSGAS RNGGSSSAAA AAAAAAAAAA ALVSDSFSCG GSPGSSAFSL
110 120 130 140 150
TSSSAAAAAA AAAAAASSSP FANDYSVFQA PGVSGGSGGG GGGGGGGSSA
160 170 180 190 200
HSQDGSHQPV FISKVHTSVD GLQGIYPRVG MAHPYESWFK PSHPGLGAAG
210 220 230 240 250
EVGSAGASSW WDVGAGWIDV QNPNSAAALP GSLHPAAGGL QTSLHSPLGG
260 270 280 290 300
YNSDYSGLSH SAFSSGASSH LLSPAGQHLM DGFKPVLPGS YPDSAPSPLA
310 320 330 340 350
GAGGSMLSAG PSAPLGGSPR SSARRYSGRA TCDCPNCQEA ERLGPAGASL
360 370 380 390 400
RRKGLHSCHI PGCGKVYGKT SHLKAHLRWH TGERPFVCNW LFCGKRFTRS
410 420 430 440 450
DELQRHLRTH TGEKRFACPV CNKRFMRSDH LSKHVKTHSG GGGGGGSAGS
460 470 480 490
GSGGKKGSDT DSEHSAAGSP PCHSPELLQP PEPGHRNGLE
Length:490
Mass (Da):48,674
Last modified:July 13, 2010 - v3
Checksum:i8FB659057C6F8E58
GO
Isoform 1 (identifier: Q8IXZ3-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     104-145: Missing.

Note: No experimental confirmation available.

Show »
Length:448
Mass (Da):45,235
Checksum:i23D3E1CE4DBBE0AE
GO
Isoform 2 (identifier: Q8IXZ3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     92-147: Missing.

Note: No experimental confirmation available.

Show »
Length:434
Mass (Da):44,012
Checksum:i8D1AC12A65A89605
GO
Isoform 4 (identifier: Q8IXZ3-4) [UniParc]FASTAAdd to basket

Also known as: Sp8L

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MATSLLGEEPRLGSTPLAM

Show »
Length:508
Mass (Da):50,500
Checksum:i99C8728AA08D139F
GO

Sequence cautioni

The sequence BAB71297.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti430 – 4301H → R in BC038669 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MATSLLGEEPRLGSTPLAM in isoform 4. 1 PublicationVSP_044094
Alternative sequencei92 – 14756Missing in isoform 2. 1 PublicationVSP_007441Add
BLAST
Alternative sequencei104 – 14542Missing in isoform 1. 1 PublicationVSP_011036Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY167047 mRNA. Translation: AAO38028.1.
AY167048 mRNA. Translation: AAO38029.1.
AK056857 mRNA. Translation: BAB71297.1. Different initiation.
CH471073 Genomic DNA. Translation: EAW93731.1.
BC038669 mRNA. No translation available.
CCDSiCCDS43555.1. [Q8IXZ3-4]
CCDS5372.1. [Q8IXZ3-3]
RefSeqiNP_874359.2. NM_182700.5. [Q8IXZ3-4]
NP_945194.1. NM_198956.3. [Q8IXZ3-3]
UniGeneiHs.195922.

Genome annotation databases

EnsembliENST00000361443; ENSP00000354482; ENSG00000164651. [Q8IXZ3-3]
ENST00000418710; ENSP00000408792; ENSG00000164651. [Q8IXZ3-4]
GeneIDi221833.
KEGGihsa:221833.
UCSCiuc003suy.3. human. [Q8IXZ3-3]
uc003suz.3. human. [Q8IXZ3-4]

Polymorphism and mutation databases

BioMutaiSP8.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY167047 mRNA. Translation: AAO38028.1.
AY167048 mRNA. Translation: AAO38029.1.
AK056857 mRNA. Translation: BAB71297.1. Different initiation.
CH471073 Genomic DNA. Translation: EAW93731.1.
BC038669 mRNA. No translation available.
CCDSiCCDS43555.1. [Q8IXZ3-4]
CCDS5372.1. [Q8IXZ3-3]
RefSeqiNP_874359.2. NM_182700.5. [Q8IXZ3-4]
NP_945194.1. NM_198956.3. [Q8IXZ3-3]
UniGeneiHs.195922.

3D structure databases

ProteinModelPortaliQ8IXZ3.
SMRiQ8IXZ3. Positions 324-447.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128760. 3 interactions.
STRINGi9606.ENSP00000408792.

PTM databases

PhosphoSiteiQ8IXZ3.

Polymorphism and mutation databases

BioMutaiSP8.
DMDMi300669678.

Proteomic databases

PaxDbiQ8IXZ3.
PRIDEiQ8IXZ3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361443; ENSP00000354482; ENSG00000164651. [Q8IXZ3-3]
ENST00000418710; ENSP00000408792; ENSG00000164651. [Q8IXZ3-4]
GeneIDi221833.
KEGGihsa:221833.
UCSCiuc003suy.3. human. [Q8IXZ3-3]
uc003suz.3. human. [Q8IXZ3-4]

Organism-specific databases

CTDi221833.
GeneCardsiGC07M020788.
HGNCiHGNC:19196. SP8.
HPAiHPA054006.
MIMi608306. gene.
neXtProtiNX_Q8IXZ3.
PharmGKBiPA134893390.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000118984.
HOGENOMiHOG000231067.
HOVERGENiHBG036899.
InParanoidiQ8IXZ3.
KOiK09198.
OMAiFGNDYSV.
OrthoDBiEOG7JQBMX.
PhylomeDBiQ8IXZ3.
TreeFamiTF350150.

Miscellaneous databases

GeneWikiiSp8_transcription_factor.
GenomeRNAii221833.
NextBioi91473.
PROiQ8IXZ3.
SOURCEiSearch...

Gene expression databases

BgeeiQ8IXZ3.
CleanExiHS_SP8.
GenevestigatoriQ8IXZ3.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic structure and cloning of two transcript isoforms of human Sp8."
    Milona M.-A., Gough J.E., Edgar A.J.
    BMC Genomics 5:86-86(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4), ALTERNATIVE SPLICING.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Prostate.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.

Entry informationi

Entry nameiSP8_HUMAN
AccessioniPrimary (citable) accession number: Q8IXZ3
Secondary accession number(s): Q7Z615, Q7Z616, Q96MJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: July 13, 2010
Last modified: April 29, 2015
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.