Q8IXZ2 (ZC3H3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger CCCH domain-containing protein 3

Gene names

Name:	ZC3H3
Synonyms:	KIAA0150, ZC3HDC3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	948 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Contains 5 C3H1-type zinc fingers.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mRNA polyadenylation Inferred from mutant phenotype. Source: UniProtKB poly(A)+ mRNA export from nucleus Inferred from mutant phenotype. Source: UniProtKB regulation of mRNA export from nucleus Inferred from mutant phenotype. Source: UniProtKB
Cellular component	nucleus Inferred from direct assay. Source: UniProtKB
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8IXZ2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8IXZ2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-613: Missing.
     614-634: NKLSKTSGQPSDAGSRPLLRT → MEPGGEPTGAKESSTLMESLA

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 948	948	Zinc finger CCCH domain-containing protein 3		PRO_0000213896
Regions
	Zinc finger	667 – 695	29	C3H1-type 1
	Zinc finger	699 – 722	24	C3H1-type 2
	Zinc finger	723 – 749	27	C3H1-type 3
	Zinc finger	750 – 777	28	C3H1-type 4
	Zinc finger	778 – 800	23	C3H1-type 5
	Compositional bias	382 – 391	10	Poly-Ser
	Compositional bias	873 – 881	9	Poly-Ser
Amino acid modifications
	Modified residue	207	1	Phosphoserine Ref.4
	Modified residue	211	1	Phosphoserine Ref.4
	Modified residue	383	1	Phosphoserine Ref.5
	Modified residue	569	1	Phosphoserine Ref.5
	Modified residue	571	1	Phosphoserine Ref.5 Ref.7
	Modified residue	617	1	Phosphoserine Ref.6
	Modified residue	917	1	Phosphoserine Ref.8
	Modified residue	918	1	Phosphoserine Ref.8
	Modified residue	920	1	Phosphoserine Ref.8
Natural variations
	Alternative sequence	1 – 613	613	Missing in isoform 2.		VSP_010272
	Alternative sequence	614 – 634	21	NKLSK…PLLRT → MEPGGEPTGAKESSTLMESL A in isoform 2.		VSP_010273
	Natural variant	6	1	I → M. Ref.2 Corresponds to variant rs2242093 [ dbSNP | Ensembl ].		VAR_018457
	Natural variant	102	1	Q → R. Ref.2 Corresponds to variant rs17857167 [ dbSNP | Ensembl ].		VAR_060402
	Natural variant	149	1	F → Y. Corresponds to variant rs3750206 [ dbSNP | Ensembl ].		VAR_018458
	Natural variant	151	1	E → D. Ref.3 Corresponds to variant rs3750207 [ dbSNP | Ensembl ].		VAR_018459
	Natural variant	165	1	E → K. Ref.2 Corresponds to variant rs17853852 [ dbSNP | Ensembl ].		VAR_060403
	Natural variant	168	1	R → W. Corresponds to variant rs3750208 [ dbSNP | Ensembl ].		VAR_057484
	Natural variant	228	1	A → S. Corresponds to variant rs4873802 [ dbSNP | Ensembl ].		VAR_057485
	Natural variant	231	1	P → A. Ref.2 Corresponds to variant rs17853853 [ dbSNP | Ensembl ].		VAR_060404
	Natural variant	351	1	P → L. Corresponds to variant rs34674128 [ dbSNP | Ensembl ].		VAR_057486
	Natural variant	399	1	S → G. Ref.3 Corresponds to variant rs1318196 [ dbSNP | Ensembl ].		VAR_018460
	Natural variant	415	1	P → L. Corresponds to variant rs36008851 [ dbSNP | Ensembl ].		VAR_057487
	Natural variant	452	1	S → G. Ref.2 Ref.3 Corresponds to variant rs4874147 [ dbSNP | Ensembl ].		VAR_018461
	Natural variant	503	1	T → K. Corresponds to variant rs11548254 [ dbSNP | Ensembl ].		VAR_057488
	Natural variant	578	1	R → H. Ref.2 Corresponds to variant rs17855618 [ dbSNP | Ensembl ].		VAR_060405
	Natural variant	636	1	R → W. Ref.2 Corresponds to variant rs17857164 [ dbSNP | Ensembl ].		VAR_060406
	Natural variant	727	1	P → R. Ref.2 Corresponds to variant rs17857168 [ dbSNP | Ensembl ].		VAR_060407
Experimental info
	Sequence conflict	572	1	R → G in AAH38670. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 5, 2010. Version 3. Checksum: 9CDD4F29EE40946B	FASTA	948	101,941
	10 20 30 40 50 60 MEEKEILRRQ IRLLQGLIDD YKTLHGNAPA PGTPAASGWQ PPTYHSGRAF SARYPRPSRR 70 80 90 100 110 120 GYSSHHGPSW RKKYSLVNRP PGPSDPPADH AVRPLHGARG GQPPVPQQHV LERQVQLSQG 130 140 150 160 170 180 QNVVIKVKPP SKSGSASASG AQRGSLEEFE ETPWSDQRPR EGEGEPPRGQ LQPSRPTRAR 190 200 210 220 230 240 GTCSVEDPLL VCQKEPGKPR MVKSVGSVGD SPREPRRTVS ESVIAVKASF PSSALPPRTG 250 260 270 280 290 300 VALGRKLGSH SVASCAPQLL GDRRVDAGHT DQPVPSGSVG GPARPASGPR QAREASLVVT 310 320 330 340 350 360 CRTNKFRKNN YKWVAASSKS PRVARRALSP RVAAENVCKA SAGMANKVEK PQLIADPEPK 370 380 390 400 410 420 PRKPATSSKP GSAPSKYKWK ASSPSASSSS SFRWQSEASS KDHASQLSPV LSRSPSGDRP 430 440 450 460 470 480 AVGHSGLKPL SGETPLSAYK VKSRTKIIRR RSSTSLPGDK KSGTSPAATA KSHLSLRRRQ 490 500 510 520 530 540 ALRGKSSPVL KKTPNKGLVQ VTTHRLCRLP PSRAHLPTKE ASSLHAVRTA PTSKVIKTRY 550 560 570 580 590 600 RIVKKTPASP LSAPPFPLSL PSWRARRLSL SRSLVLNRLR PVASGGGKAQ PGSPWWRSKG 610 620 630 640 650 660 YRCIGGVLYK VSANKLSKTS GQPSDAGSRP LLRTGRLDPA GSCSRSLASR AVQRSLAIIR 670 680 690 700 710 720 QARQRREKRK EYCMYYNRFG RCNRGERCPY IHDPEKVAVC TRFVRGTCKK TDGTCPFSHH 730 740 750 760 770 780 VSKEKMPVCS YFLKGICSNS NCPYSHVYVS RKAEVCSDFL KGYCPLGAKC KKKHTLLCPD 790 800 810 820 830 840 FARRGACPRG AQCQLLHRTQ KRHSRRAATS PAPGPSDATA RSRVSASHGP RKPSASQRPT 850 860 870 880 890 900 RQTPSSAALT AAAVAAPPHC PGGSASPSSS KASSSSSSSS SPPASLDHEA PSLQEAALAA 910 920 930 940 ACSNRLCKLP SFISLQSSPS PGAQPRVRAP RAPLTKDSGK PLHIKPRL
	Isoform 2 [UniParc]. Checksum: B5FE8363BA91A4C3	FASTA	335	35,929
	10 20 30 40 50 60 MEPGGEPTGA KESSTLMESL AGRLDPAGSC SRSLASRAVQ RSLAIIRQAR QRREKRKEYC 70 80 90 100 110 120 MYYNRFGRCN RGERCPYIHD PEKVAVCTRF VRGTCKKTDG TCPFSHHVSK EKMPVCSYFL 130 140 150 160 170 180 KGICSNSNCP YSHVYVSRKA EVCSDFLKGY CPLGAKCKKK HTLLCPDFAR RGACPRGAQC 190 200 210 220 230 240 QLLHRTQKRH SRRAATSPAP GPSDATARSR VSASHGPRKP SASQRPTRQT PSSAALTAAA 250 260 270 280 290 300 VAAPPHCPGG SASPSSSKAS SSSSSSSSPP ASLDHEAPSL QEAALAAACS NRLCKLPSFI 310 320 330 SLQSSPSPGA QPRVRAPRAP LTKDSGKPLH IKPRL

References

[1]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed: 16421571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANTS MET-6; ARG-102; LYS-165; ALA-231; GLY-452; HIS-578; TRP-636 AND ARG-727. Tissue: Brain.
[3]	"Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1." Nagase T., Seki N., Tanaka A., Ishikawa K., Nomura N. DNA Res. 2:167-174(1995) [PubMed: 8590280] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-948 (ISOFORM 1), VARIANTS ASP-151; GLY-399 AND GLY-452. Tissue: Bone marrow.
[4]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-207 AND SER-211, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[5]	"Evaluation of the low-specificity protease elastase for large-scale phosphoproteome analysis." Wang B., Malik R., Nigg E.A., Korner R. Anal. Chem. 80:9526-9533(2008) [PubMed: 19007248] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-383; SER-569 AND SER-571, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-617, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-571, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-917; SER-918 AND SER-920, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC067930 Genomic DNA. No translation available. AC105118 Genomic DNA. No translation available. BC034435 mRNA. Translation: AAH34435.1. BC038670 mRNA. Translation: AAH38670.1. D63484 mRNA. Translation: BAA09771.1.
IPI	IPI00384232. IPI00410013.
RefSeq	NP_055932.2. NM_015117.2.
UniGene	Hs.521915.
3D structure databases
ProteinModelPortal	Q8IXZ2.
SMR	Q8IXZ2. Positions 667-800.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8IXZ2. 1 interaction.
STRING	Q8IXZ2.
PTM databases
PhosphoSite	Q8IXZ2.
Polymorphism databases
DMDM	308153538.
Proteomic databases
PRIDE	Q8IXZ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000262577; ENSP00000262577; ENSG00000014164.
GeneID	23144.
KEGG	hsa:23144.
UCSC	uc003yyd.2. human.
Organism-specific databases
CTD	23144.
GeneCards	GC08M144519.
H-InvDB	HIX0022677.
HGNC	HGNC:28972. ZC3H3.
HPA	HPA023658.
neXtProt	NX_Q8IXZ2.
PharmGKB	PA134933089.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG18148.
GeneTree	ENSGT00390000009627.
HOGENOM	HBG283372.
HOVERGEN	HBG055611.
InParanoid	Q8IXZ2.
OMA	EDPLLVC.
OrthoDB	EOG40ZQX1.
PhylomeDB	Q8IXZ2.
Gene expression databases
ArrayExpress	Q8IXZ2.
Bgee	Q8IXZ2.
CleanEx	HS_ZC3H3.
Genevestigator	Q8IXZ2.
GermOnline	ENSG00000014164. Homo sapiens.
Family and domain databases
InterPro	IPR000571. Znf_CCCH. [Graphical view]
Pfam	PF00642. zf-CCCH. 2 hits. [Graphical view]
SMART	SM00356. ZnF_C3H1. 5 hits. [Graphical view]
PROSITE	PS50103. ZF_C3H1. 5 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	44434.

Entry information

Entry name

ZC3H3_HUMAN

Accession

Primary (citable) accession number: Q8IXZ2
Secondary accession number(s): Q14163, Q8N4E2, Q9BUS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	October 5, 2010
Last modified:	January 25, 2012
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.