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Protein

Poly [ADP-ribose] polymerase 9

Gene

PARP9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In concert with DTX3L plays a role in PARP1-dependent DNA damage repair. PARP1-dependent PARP9/BAL1-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. Involved in inducing the expression of IFN-gamma-responsive genes.2 Publications

Catalytic activityi

NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.PROSITE-ProRule annotation

GO - Molecular functioni

  • NAD+ ADP-ribosyltransferase activity Source: UniProtKB

GO - Biological processi

  • cell migration Source: UniProtKB
  • double-strand break repair Source: UniProtKB
  • regulation of response to interferon-gamma Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciZFISH:HS13730-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Poly [ADP-ribose] polymerase 9 (EC:2.4.2.30)
Short name:
PARP-9
Alternative name(s):
ADP-ribosyltransferase diphtheria toxin-like 9
Short name:
ARTD9
B aggressive lymphoma protein
Gene namesi
Name:PARP9
Synonyms:BAL, BAL1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:24118. PARP9.

Subcellular locationi

  • Cytoplasmcytosol
  • Nucleus

  • Note: Shuttles between the nucleus and the cytosol. Export to the cytosol depends on the interaction with DTX3L. Localizes at sites of DNA damage in a PARP1-dependent manner.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: UniProtKB-SubCell
  • membrane Source: UniProtKB
  • mitochondrion Source: HPA
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

Overexpressed at significantly higher levels in fatal high-risk diffuse large B-cell lymphomas (DLB-CL) compared to cured low-risk tumors. Overexpression in B-cell lymphoma transfectants may promote malignant B-cell migration. May therefore be involved in promoting B-cell migration and dissemination of high-risk DLB-CL tumors (PubMed:11110709).

Organism-specific databases

DisGeNETi83666.
OpenTargetsiENSG00000138496.
PharmGKBiPA134870403.

Polymorphism and mutation databases

BioMutaiPARP9.
DMDMi48474734.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002113391 – 854Poly [ADP-ribose] polymerase 9Add BLAST854

Proteomic databases

EPDiQ8IXQ6.
MaxQBiQ8IXQ6.
PaxDbiQ8IXQ6.
PeptideAtlasiQ8IXQ6.
PRIDEiQ8IXQ6.

PTM databases

iPTMnetiQ8IXQ6.
PhosphoSitePlusiQ8IXQ6.

Expressioni

Tissue specificityi

Expressed in lymphocyte-rich tissues, spleen, lymph nodes, peripheral blood lymphocytes and colonic mucosa. Also expressed in nonhematopoietic tissues such as heart and skeletal muscle. Isoform 2 is the predominant form. Most abundantly expressed in lymphomas with a brisk host inflammatory response. In diffuse large B-cell lymphomas tumors, expressed specifically by malignant B-cells.2 Publications

Inductioni

Up-regulated by IFN-gamma in B-cell lymphoma cell lines.1 Publication

Gene expression databases

BgeeiENSG00000138496.
CleanExiHS_PARP9.
ExpressionAtlasiQ8IXQ6. baseline and differential.
GenevisibleiQ8IXQ6. HS.

Organism-specific databases

HPAiHPA066708.

Interactioni

Subunit structurei

Interacts with DTX3L. Interacts with PARP1 (when poly-ADP-ribosylated).2 Publications

Protein-protein interaction databases

BioGridi123723. 10 interactors.
IntActiQ8IXQ6. 1 interactor.
STRINGi9606.ENSP00000353512.

Structurei

Secondary structure

1854
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi311 – 314Combined sources4
Beta strandi317 – 324Combined sources8
Helixi326 – 328Combined sources3
Beta strandi330 – 339Combined sources10
Helixi347 – 356Combined sources10
Helixi358 – 370Combined sources13
Beta strandi378 – 382Combined sources5
Beta strandi386 – 395Combined sources10
Helixi402 – 419Combined sources18
Beta strandi423 – 427Combined sources5
Beta strandi433 – 437Combined sources5
Helixi439 – 456Combined sources18
Beta strandi462 – 468Combined sources7
Helixi473 – 490Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5AILX-ray1.55A/B310-493[»]
ProteinModelPortaliQ8IXQ6.
SMRiQ8IXQ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini107 – 296Macro 1PROSITE-ProRule annotationAdd BLAST190
Domaini306 – 487Macro 2PROSITE-ProRule annotationAdd BLAST182
Domaini628 – 850PARP catalyticPROSITE-ProRule annotationAdd BLAST223

Domaini

The domain macro 2 is required for recruitment to DNA damage sites.1 Publication

Sequence similaritiesi

Contains 2 Macro domains.PROSITE-ProRule annotation
Contains 1 PARP catalytic domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2633. Eukaryota.
COG2110. LUCA.
GeneTreeiENSGT00760000119084.
HOGENOMiHOG000115452.
HOVERGENiHBG045406.
InParanoidiQ8IXQ6.
KOiK15260.
OMAiSGIFQFP.
OrthoDBiEOG091G02AK.
PhylomeDBiQ8IXQ6.
TreeFamiTF328965.

Family and domain databases

Gene3Di3.90.228.10. 1 hit.
InterProiIPR002589. Macro_dom.
IPR012317. Poly(ADP-ribose)pol_cat_dom.
[Graphical view]
PfamiPF01661. Macro. 2 hits.
[Graphical view]
SMARTiSM00506. A1pp. 2 hits.
[Graphical view]
PROSITEiPS51154. MACRO. 2 hits.
PS51059. PARP_CATALYTIC. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8IXQ6-1) [UniParc]FASTAAdd to basket
Also known as: Long, L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDFSMVAGAA AYNEKSGRIT SLSLLFQKVF AQIFPQWRKG NTEECLPYKC
60 70 80 90 100
SETGALGENY SWQIPINHND FKILKNNERQ LCEVLQNKFG CISTLVSPVQ
110 120 130 140 150
EGNSKSLQVF RKMLTPRIEL SVWKDDLTTH AVDAVVNAAN EDLLHGGGLA
160 170 180 190 200
LALVKAGGFE IQEESKQFVA RYGKVSAGEI AVTGAGRLPC KQIIHAVGPR
210 220 230 240 250
WMEWDKQGCT GKLQRAIVSI LNYVIYKNTH IKTVAIPALS SGIFQFPLNL
260 270 280 290 300
CTKTIVETIR VSLQGKPMMS NLKEIHLVSN EDPTVAAFKA ASEFILGKSE
310 320 330 340 350
LGQETTPSFN AMVVNNLTLQ IVQGHIEWQT ADVIVNSVNP HDITVGPVAK
360 370 380 390 400
SILQQAGVEM KSEFLATKAK QFQRSQLVLV TKGFNLFCKY IYHVLWHSEF
410 420 430 440 450
PKPQILKHAM KECLEKCIEQ NITSISFPAL GTGNMEIKKE TAAEILFDEV
460 470 480 490 500
LTFAKDHVKH QLTVKFVIFP TDLEIYKAFS SEMAKRSKML SLNNYSVPQS
510 520 530 540 550
TREEKRENGL EARSPAINLM GFNVEEMYEA HAWIQRILSL QNHHIIENNH
560 570 580 590 600
ILYLGRKEHD ILSQLQKTSS VSITEIISPG RTELEIEGAR ADLIEVVMNI
610 620 630 640 650
EDMLCKVQEE MARKKERGLW RSLGQWTIQQ QKTQDEMKEN IIFLKCPVPP
660 670 680 690 700
TQELLDQKKQ FEKCGLQVLK VEKIDNEVLM AAFQRKKKMM EEKLHRQPVS
710 720 730 740 750
HRLFQQVPYQ FCNVVCRVGF QRMYSTPCDP KYGAGIYFTK NLKNLAEKAK
760 770 780 790 800
KISAADKLIY VFEAEVLTGF FCQGHPLNIV PPPLSPGAID GHDSVVDNVS
810 820 830 840 850
SPETFVIFSG MQAIPQYLWT CTQEYVQSQD YSSGPMRPFA QHPWRGFASG

SPVD
Length:854
Mass (Da):96,343
Last modified:October 10, 2003 - v2
Checksum:iCBBB3B4B7CA1CDA4
GO
Isoform 2 (identifier: Q8IXQ6-2) [UniParc]FASTAAdd to basket
Also known as: Short, S

The sequence of this isoform differs from the canonical sequence as follows:
     17-51: Missing.

Show »
Length:819
Mass (Da):92,271
Checksum:i110B81666B857940
GO
Isoform 3 (identifier: Q8IXQ6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     17-51: Missing.
     729-745: DPKYGAGIYFTKNLKNL → GRCQCLIIGATLWNLVS
     746-854: Missing.

Note: No experimental confirmation available.
Show »
Length:710
Mass (Da):80,291
Checksum:iE443D3E78BDF0E53
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti487S → P in BAF85648 (PubMed:14702039).Curated1
Sequence conflicti839F → S in BAG36276 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05665421S → L.Corresponds to variant rs34006803dbSNPEnsembl.1
Natural variantiVAR_056655517I → V.1 PublicationCorresponds to variant rs28365795dbSNPEnsembl.1
Natural variantiVAR_056656528Y → C.3 PublicationsCorresponds to variant rs9851180dbSNPEnsembl.1
Natural variantiVAR_056657651T → A.Corresponds to variant rs6780543dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00850517 – 51Missing in isoform 2 and isoform 3. 3 PublicationsAdd BLAST35
Alternative sequenceiVSP_046086729 – 745DPKYG…NLKNL → GRCQCLIIGATLWNLVS in isoform 3. 1 PublicationAdd BLAST17
Alternative sequenceiVSP_046087746 – 854Missing in isoform 3. 1 PublicationAdd BLAST109

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF307338 mRNA. Translation: AAK07558.1.
AF307339 mRNA. Translation: AAK07559.1.
AK292959 mRNA. Translation: BAF85648.1.
AK313494 mRNA. Translation: BAG36276.1.
AC092908 Genomic DNA. No translation available.
AC096861 Genomic DNA. No translation available.
BC017463 mRNA. No translation available.
BC039580 mRNA. Translation: AAH39580.1.
AL713679 mRNA. Translation: CAD28483.1.
CCDSiCCDS3014.1. [Q8IXQ6-1]
CCDS54633.1. [Q8IXQ6-3]
CCDS54634.1. [Q8IXQ6-2]
RefSeqiNP_001139574.1. NM_001146102.1. [Q8IXQ6-1]
NP_001139575.1. NM_001146103.1. [Q8IXQ6-2]
NP_001139576.1. NM_001146104.1. [Q8IXQ6-2]
NP_001139577.1. NM_001146105.1. [Q8IXQ6-2]
NP_113646.2. NM_031458.2. [Q8IXQ6-1]
XP_005247877.1. XM_005247820.2. [Q8IXQ6-1]
XP_016862793.1. XM_017007304.1. [Q8IXQ6-1]
XP_016862794.1. XM_017007305.1. [Q8IXQ6-2]
XP_016862795.1. XM_017007306.1. [Q8IXQ6-2]
UniGeneiHs.518200.

Genome annotation databases

EnsembliENST00000360356; ENSP00000353512; ENSG00000138496. [Q8IXQ6-1]
ENST00000462315; ENSP00000418894; ENSG00000138496. [Q8IXQ6-3]
ENST00000471785; ENSP00000419001; ENSG00000138496. [Q8IXQ6-2]
ENST00000477522; ENSP00000419506; ENSG00000138496. [Q8IXQ6-2]
GeneIDi83666.
KEGGihsa:83666.
UCSCiuc003efh.5. human. [Q8IXQ6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF307338 mRNA. Translation: AAK07558.1.
AF307339 mRNA. Translation: AAK07559.1.
AK292959 mRNA. Translation: BAF85648.1.
AK313494 mRNA. Translation: BAG36276.1.
AC092908 Genomic DNA. No translation available.
AC096861 Genomic DNA. No translation available.
BC017463 mRNA. No translation available.
BC039580 mRNA. Translation: AAH39580.1.
AL713679 mRNA. Translation: CAD28483.1.
CCDSiCCDS3014.1. [Q8IXQ6-1]
CCDS54633.1. [Q8IXQ6-3]
CCDS54634.1. [Q8IXQ6-2]
RefSeqiNP_001139574.1. NM_001146102.1. [Q8IXQ6-1]
NP_001139575.1. NM_001146103.1. [Q8IXQ6-2]
NP_001139576.1. NM_001146104.1. [Q8IXQ6-2]
NP_001139577.1. NM_001146105.1. [Q8IXQ6-2]
NP_113646.2. NM_031458.2. [Q8IXQ6-1]
XP_005247877.1. XM_005247820.2. [Q8IXQ6-1]
XP_016862793.1. XM_017007304.1. [Q8IXQ6-1]
XP_016862794.1. XM_017007305.1. [Q8IXQ6-2]
XP_016862795.1. XM_017007306.1. [Q8IXQ6-2]
UniGeneiHs.518200.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5AILX-ray1.55A/B310-493[»]
ProteinModelPortaliQ8IXQ6.
SMRiQ8IXQ6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123723. 10 interactors.
IntActiQ8IXQ6. 1 interactor.
STRINGi9606.ENSP00000353512.

PTM databases

iPTMnetiQ8IXQ6.
PhosphoSitePlusiQ8IXQ6.

Polymorphism and mutation databases

BioMutaiPARP9.
DMDMi48474734.

Proteomic databases

EPDiQ8IXQ6.
MaxQBiQ8IXQ6.
PaxDbiQ8IXQ6.
PeptideAtlasiQ8IXQ6.
PRIDEiQ8IXQ6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000360356; ENSP00000353512; ENSG00000138496. [Q8IXQ6-1]
ENST00000462315; ENSP00000418894; ENSG00000138496. [Q8IXQ6-3]
ENST00000471785; ENSP00000419001; ENSG00000138496. [Q8IXQ6-2]
ENST00000477522; ENSP00000419506; ENSG00000138496. [Q8IXQ6-2]
GeneIDi83666.
KEGGihsa:83666.
UCSCiuc003efh.5. human. [Q8IXQ6-1]

Organism-specific databases

CTDi83666.
DisGeNETi83666.
GeneCardsiPARP9.
HGNCiHGNC:24118. PARP9.
HPAiHPA066708.
MIMi612065. gene.
neXtProtiNX_Q8IXQ6.
OpenTargetsiENSG00000138496.
PharmGKBiPA134870403.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2633. Eukaryota.
COG2110. LUCA.
GeneTreeiENSGT00760000119084.
HOGENOMiHOG000115452.
HOVERGENiHBG045406.
InParanoidiQ8IXQ6.
KOiK15260.
OMAiSGIFQFP.
OrthoDBiEOG091G02AK.
PhylomeDBiQ8IXQ6.
TreeFamiTF328965.

Enzyme and pathway databases

BioCyciZFISH:HS13730-MONOMER.

Miscellaneous databases

GenomeRNAii83666.
PROiQ8IXQ6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000138496.
CleanExiHS_PARP9.
ExpressionAtlasiQ8IXQ6. baseline and differential.
GenevisibleiQ8IXQ6. HS.

Family and domain databases

Gene3Di3.90.228.10. 1 hit.
InterProiIPR002589. Macro_dom.
IPR012317. Poly(ADP-ribose)pol_cat_dom.
[Graphical view]
PfamiPF01661. Macro. 2 hits.
[Graphical view]
SMARTiSM00506. A1pp. 2 hits.
[Graphical view]
PROSITEiPS51154. MACRO. 2 hits.
PS51059. PARP_CATALYTIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPARP9_HUMAN
AccessioniPrimary (citable) accession number: Q8IXQ6
Secondary accession number(s): A8KA94
, B2R8S9, E9PFM7, Q8TCP3, Q9BZL8, Q9BZL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: October 10, 2003
Last modified: November 2, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.