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Protein

Polyhomeotic-like protein 2

Gene

PHC2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri633 – 667FCS-typePROSITE-ProRule annotationAdd BLAST35

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-2559580. Oxidative Stress Induced Senescence.
R-HSA-3108214. SUMOylation of DNA damage response and repair proteins.
R-HSA-4570464. SUMOylation of RNA binding proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyhomeotic-like protein 2
Short name:
hPH2
Alternative name(s):
Early development regulatory protein 2
Gene namesi
Name:PHC2
Synonyms:EDR2, PH2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:3183. PHC2.

Subcellular locationi

GO - Cellular componenti

  • heterochromatin Source: Ensembl
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • PcG protein complex Source: UniProtKB
  • PRC1 complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi1912.
OpenTargetsiENSG00000134686.
PharmGKBiPA27620.

Polymorphism and mutation databases

BioMutaiPHC2.
DMDMi74750731.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000762861 – 858Polyhomeotic-like protein 2Add BLAST858

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei619PhosphothreonineCombined sources1
Modified residuei621PhosphoserineCombined sources1
Modified residuei751PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8IXK0.
MaxQBiQ8IXK0.
PaxDbiQ8IXK0.
PeptideAtlasiQ8IXK0.
PRIDEiQ8IXK0.

PTM databases

iPTMnetiQ8IXK0.
PhosphoSitePlusiQ8IXK0.

Expressioni

Gene expression databases

BgeeiENSG00000134686.
ExpressionAtlasiQ8IXK0. baseline and differential.
GenevisibleiQ8IXK0. HS.

Organism-specific databases

HPAiHPA047403.

Interactioni

Subunit structurei

Component of a PRC1-like complex. Interacts with CBX4. Interacts with BMI1, PCGF2, PHC1 and RNF2. Interacts with CHTOP (By similarity). Interacts with the N-terminal region of the SP1 transcription factor and with MAPKAPK2.By similarity8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-713786,EBI-713786
AENQ8WTP83EBI-713786,EBI-8637627
AP1M1Q9BXS53EBI-713786,EBI-541426
BMI1P352267EBI-713786,EBI-2341576
Bmi1P259162EBI-713786,EBI-927401From a different organism.
BYSLQ138956EBI-713786,EBI-358049
C1orf109Q9NX043EBI-713786,EBI-8643161
CARD9Q9H2573EBI-713786,EBI-751319
CBX8Q9HC527EBI-713786,EBI-712912
DRG1Q9Y2954EBI-713786,EBI-719554
ENKD1Q9H0I24EBI-713786,EBI-744099
FAM124AQ86V423EBI-713786,EBI-744506
FAM13CQ8NE313EBI-713786,EBI-751248
FAM161AQ3B8203EBI-713786,EBI-719941
FAM74A2Q5RGS33EBI-713786,EBI-10244822
FAM74A6Q5TZK33EBI-713786,EBI-10247271
KAT5Q929933EBI-713786,EBI-399080
KBTBD7Q8WVZ93EBI-713786,EBI-473695
KIFC3Q9BVG83EBI-713786,EBI-2125614
L3MBTL3Q96JM76EBI-713786,EBI-2686809
LMO1P258003EBI-713786,EBI-8639312
LMO2P257914EBI-713786,EBI-739696
MAB21L3Q8N8X93EBI-713786,EBI-10268010
MAGEB6Q8N7X43EBI-713786,EBI-6447163
MCRS1Q96EZ82EBI-713786,EBI-348259
MFAP1P550813EBI-713786,EBI-1048159
MORF4L2Q150143EBI-713786,EBI-399257
PCGF2P352278EBI-713786,EBI-2129767
PCGF3Q3KNV83EBI-713786,EBI-2339807
PCGF5Q86SE93EBI-713786,EBI-2827999
PLK1P533502EBI-713786,EBI-476768
POLR2LP628753EBI-713786,EBI-359527
PPP1R16BQ96T493EBI-713786,EBI-10293968
PRKAA1Q131313EBI-713786,EBI-1181405
PRPF31F1T0A53EBI-713786,EBI-10177194
RBM39Q144983EBI-713786,EBI-395290
RBM39Q14498-33EBI-713786,EBI-6654703
RWDD2BP570603EBI-713786,EBI-724442
SDCBPO005603EBI-713786,EBI-727004
SFMBT1Q9UHJ34EBI-713786,EBI-747398
SIAH1Q8IUQ45EBI-713786,EBI-747107
SMAD3P840223EBI-713786,EBI-347161
SP1P080472EBI-713786,EBI-298336
SPATC1LQ9H0A93EBI-10304199,EBI-372911
SSX2IPQ9Y2D83EBI-713786,EBI-2212028
SYT16Q17RD73EBI-713786,EBI-10238936
THAP7Q9BT493EBI-713786,EBI-741350
TRIM41Q8WV443EBI-713786,EBI-725997
ZBTB24O431673EBI-713786,EBI-744471
ZGPATQ8N5A53EBI-713786,EBI-3439227
ZGPATQ8N5A5-23EBI-713786,EBI-10183064
ZMAT2Q96NC03EBI-713786,EBI-2682299
ZNF417Q8TAU33EBI-713786,EBI-740727

Protein-protein interaction databases

BioGridi108234. 95 interactors.
DIPiDIP-34464N.
IntActiQ8IXK0. 90 interactors.
MINTiMINT-1369031.
STRINGi9606.ENSP00000257118.

Structurei

3D structure databases

ProteinModelPortaliQ8IXK0.
SMRiQ8IXK0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini794 – 858SAMPROSITE-ProRule annotationAdd BLAST65

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi558 – 587HD1Add BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi11 – 37Ser-richAdd BLAST27
Compositional biasi55 – 111Gln-richAdd BLAST57
Compositional biasi329 – 377Gln-richAdd BLAST49

Domaini

HD1 motif interacts with SAM domain of PHC1.

Sequence similaritiesi

Contains 1 FCS-type zinc finger.PROSITE-ProRule annotation
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri633 – 667FCS-typePROSITE-ProRule annotationAdd BLAST35

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IDZN. Eukaryota.
ENOG4111F0Y. LUCA.
GeneTreeiENSGT00550000074459.
HOGENOMiHOG000236275.
HOVERGENiHBG039325.
InParanoidiQ8IXK0.
KOiK11457.
OMAiQSRNTAQ.
OrthoDBiEOG091G02VS.
PhylomeDBiQ8IXK0.
TreeFamiTF331299.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
InterProiIPR001660. SAM.
IPR013761. SAM/pointed.
IPR012313. Znf_FCS.
[Graphical view]
PfamiPF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS50105. SAM_DOMAIN. 1 hit.
PS51024. ZF_FCS. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8IXK0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MENELPVPHT SSSACATSST SGASSSSGCN NSSSGGSGRP TGPQISVYSG
60 70 80 90 100
IPDRQTVQVI QQALHRQPST AAQYLQQMYA AQQQHLMLQT AALQQQHLSS
110 120 130 140 150
AQLQSLAAVQ QASLVSNRQG STSGSNVSAQ APAQSSSINL AASPAAAQLL
160 170 180 190 200
NRAQSVNSAA ASGIAQQAVL LGNTSSPALT ASQAQMYLRA QMLIFTPTAT
210 220 230 240 250
VATVQPELGT GSPARPPTPA QVQNLTLRTQ QTPAAAASGP TPTQPVLPSL
260 270 280 290 300
ALKPTPGGSQ PLPTPAQSRN TAQASPAGAK PGIADSVMEP HKKGDGNSSV
310 320 330 340 350
PGSMEGRAGL SRTVPAVAAH PLIAPAYAQL QPHQLLPQPS SKHLQPQFVI
360 370 380 390 400
QQQPQPQQQQ PPPQQSRPVL QAEPHPQLAS VSPSVALQPS SEAHAMPLGP
410 420 430 440 450
VTPALPLQCP TANLHKPGGS QQCHPPTPDT GPQNGHPEGV PHTPQRRFQH
460 470 480 490 500
TSAVILQLQP ASPPQQCVPD DWKEVAPGEK SVPETRSGPS PHQQAIVTAM
510 520 530 540 550
PGGLPVPTSP NIQPSPAHET GQGIVHALTD LSSPGMTSGN GNSASSIAGT
560 570 580 590 600
APQNGENKPP QAIVKPQILT HVIEGFVIQE GAEPFPVGRS SLLVGNLKKK
610 620 630 640 650
YAQGFLPEKL PQQDHTTTTD SEMEEPYLQE SKEEGAPLKL KCELCGRVDF
660 670 680 690 700
AYKFKRSKRF CSMACAKRYN VGCTKRVGLF HSDRSKLQKA GAATHNRRRA
710 720 730 740 750
SKASLPPLTK DTKKQPTGTV PLSVTAALQL THSQEDSSRC SDNSSYEEPL
760 770 780 790 800
SPISASSSTS RRRQGQRDLE LPDMHMRDLV GMGHHFLPSE PTKWNVEDVY
810 820 830 840 850
EFIRSLPGCQ EIAEEFRAQE IDGQALLLLK EDHLMSAMNI KLGPALKIYA

RISMLKDS
Length:858
Mass (Da):90,713
Last modified:March 1, 2003 - v1
Checksum:i2E3FD3898CFD702B
GO
Isoform 2 (identifier: Q8IXK0-2) [UniParc]FASTAAdd to basket
Also known as: isoform b

The sequence of this isoform differs from the canonical sequence as follows:
     1-535: Missing.

Show »
Length:323
Mass (Da):35,760
Checksum:i3D469465CA2C39AD
GO
Isoform 3 (identifier: Q8IXK0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-585: Missing.
     586-586: P → M
     809-852: CQEIAEEFRA...GPALKIYARI → LPSSFPKGHE...WPVLRPRPHL
     853-858: Missing.

Show »
Length:267
Mass (Da):30,053
Checksum:i2E784C87AA459AD0
GO
Isoform 4 (identifier: Q8IXK0-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     193-221: Missing.

Note: No experimental confirmation available.
Show »
Length:829
Mass (Da):87,812
Checksum:iFFAF6D7224ADBD72
GO
Isoform 5 (identifier: Q8IXK0-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     463-463: P → PV

Note: No experimental confirmation available.
Show »
Length:859
Mass (Da):90,812
Checksum:i7F6C839B758194DC
GO

Sequence cautioni

The sequence AAH68573 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAH92492 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAI13956 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti745S → N in BAC87622 (PubMed:14702039).Curated1
Sequence conflicti778D → E in BAC87622 (PubMed:14702039).Curated1
Sequence conflicti813A → E in AAH28396 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051276254P → S.Corresponds to variant rs10914692dbSNPEnsembl.1
Natural variantiVAR_051277475V → M.1 PublicationCorresponds to variant rs12026290dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0169141 – 585Missing in isoform 3. 1 PublicationAdd BLAST585
Alternative sequenceiVSP_0169151 – 535Missing in isoform 2. 1 PublicationAdd BLAST535
Alternative sequenceiVSP_027217193 – 221Missing in isoform 4. 1 PublicationAdd BLAST29
Alternative sequenceiVSP_039755463P → PV in isoform 5. 2 Publications1
Alternative sequenceiVSP_016916586P → M in isoform 3. 1 Publication1
Alternative sequenceiVSP_016917809 – 852CQEIA…IYARI → LPSSFPKGHETSIYSLSKHL RHSRFPQSPSGSCLWPVLRP RPHL in isoform 3. 1 PublicationAdd BLAST44
Alternative sequenceiVSP_016918853 – 858Missing in isoform 3. 1 Publication6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ419231 mRNA. Translation: CAD11673.1.
AK128821 mRNA. Translation: BAC87622.1.
AK292905 mRNA. Translation: BAF85594.1.
AL513327 Genomic DNA. Translation: CAI13955.1.
AL513327 Genomic DNA. Translation: CAI13956.1. Sequence problems.
AL513327 Genomic DNA. Translation: CAI13957.1.
CH471059 Genomic DNA. Translation: EAX07457.1.
CH471059 Genomic DNA. Translation: EAX07458.1.
CH471059 Genomic DNA. Translation: EAX07459.1.
BC015450 mRNA. Translation: AAH15450.1.
BC028396 mRNA. Translation: AAH28396.3.
BC029269 mRNA. Translation: AAH29269.1.
BC068573 mRNA. Translation: AAH68573.1. Different initiation.
BC092492 mRNA. Translation: AAH92492.1. Different initiation.
BC110863 mRNA. Translation: AAI10864.2.
BC130630 mRNA. Translation: AAI30631.1.
AJ242730 mRNA. Translation: CAB44779.1.
CCDSiCCDS378.1. [Q8IXK0-1]
CCDS379.1. [Q8IXK0-2]
RefSeqiNP_004418.2. NM_004427.3. [Q8IXK0-2]
NP_932157.1. NM_198040.2. [Q8IXK0-1]
XP_005270627.1. XM_005270570.1. [Q8IXK0-2]
XP_016856005.1. XM_017000516.1. [Q8IXK0-5]
XP_016856006.1. XM_017000517.1. [Q8IXK0-5]
XP_016856007.1. XM_017000518.1. [Q8IXK0-5]
UniGeneiHs.524271.
Hs.674410.

Genome annotation databases

EnsembliENST00000257118; ENSP00000257118; ENSG00000134686. [Q8IXK0-1]
ENST00000373418; ENSP00000362517; ENSG00000134686. [Q8IXK0-2]
GeneIDi1912.
KEGGihsa:1912.
UCSCiuc001bxe.2. human. [Q8IXK0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ419231 mRNA. Translation: CAD11673.1.
AK128821 mRNA. Translation: BAC87622.1.
AK292905 mRNA. Translation: BAF85594.1.
AL513327 Genomic DNA. Translation: CAI13955.1.
AL513327 Genomic DNA. Translation: CAI13956.1. Sequence problems.
AL513327 Genomic DNA. Translation: CAI13957.1.
CH471059 Genomic DNA. Translation: EAX07457.1.
CH471059 Genomic DNA. Translation: EAX07458.1.
CH471059 Genomic DNA. Translation: EAX07459.1.
BC015450 mRNA. Translation: AAH15450.1.
BC028396 mRNA. Translation: AAH28396.3.
BC029269 mRNA. Translation: AAH29269.1.
BC068573 mRNA. Translation: AAH68573.1. Different initiation.
BC092492 mRNA. Translation: AAH92492.1. Different initiation.
BC110863 mRNA. Translation: AAI10864.2.
BC130630 mRNA. Translation: AAI30631.1.
AJ242730 mRNA. Translation: CAB44779.1.
CCDSiCCDS378.1. [Q8IXK0-1]
CCDS379.1. [Q8IXK0-2]
RefSeqiNP_004418.2. NM_004427.3. [Q8IXK0-2]
NP_932157.1. NM_198040.2. [Q8IXK0-1]
XP_005270627.1. XM_005270570.1. [Q8IXK0-2]
XP_016856005.1. XM_017000516.1. [Q8IXK0-5]
XP_016856006.1. XM_017000517.1. [Q8IXK0-5]
XP_016856007.1. XM_017000518.1. [Q8IXK0-5]
UniGeneiHs.524271.
Hs.674410.

3D structure databases

ProteinModelPortaliQ8IXK0.
SMRiQ8IXK0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108234. 95 interactors.
DIPiDIP-34464N.
IntActiQ8IXK0. 90 interactors.
MINTiMINT-1369031.
STRINGi9606.ENSP00000257118.

PTM databases

iPTMnetiQ8IXK0.
PhosphoSitePlusiQ8IXK0.

Polymorphism and mutation databases

BioMutaiPHC2.
DMDMi74750731.

Proteomic databases

EPDiQ8IXK0.
MaxQBiQ8IXK0.
PaxDbiQ8IXK0.
PeptideAtlasiQ8IXK0.
PRIDEiQ8IXK0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000257118; ENSP00000257118; ENSG00000134686. [Q8IXK0-1]
ENST00000373418; ENSP00000362517; ENSG00000134686. [Q8IXK0-2]
GeneIDi1912.
KEGGihsa:1912.
UCSCiuc001bxe.2. human. [Q8IXK0-1]

Organism-specific databases

CTDi1912.
DisGeNETi1912.
GeneCardsiPHC2.
HGNCiHGNC:3183. PHC2.
HPAiHPA047403.
MIMi602979. gene.
neXtProtiNX_Q8IXK0.
OpenTargetsiENSG00000134686.
PharmGKBiPA27620.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IDZN. Eukaryota.
ENOG4111F0Y. LUCA.
GeneTreeiENSGT00550000074459.
HOGENOMiHOG000236275.
HOVERGENiHBG039325.
InParanoidiQ8IXK0.
KOiK11457.
OMAiQSRNTAQ.
OrthoDBiEOG091G02VS.
PhylomeDBiQ8IXK0.
TreeFamiTF331299.

Enzyme and pathway databases

ReactomeiR-HSA-2559580. Oxidative Stress Induced Senescence.
R-HSA-3108214. SUMOylation of DNA damage response and repair proteins.
R-HSA-4570464. SUMOylation of RNA binding proteins.

Miscellaneous databases

ChiTaRSiPHC2. human.
GeneWikiiPHC2.
GenomeRNAii1912.
PROiQ8IXK0.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000134686.
ExpressionAtlasiQ8IXK0. baseline and differential.
GenevisibleiQ8IXK0. HS.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
InterProiIPR001660. SAM.
IPR013761. SAM/pointed.
IPR012313. Znf_FCS.
[Graphical view]
PfamiPF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS50105. SAM_DOMAIN. 1 hit.
PS51024. ZF_FCS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHC2_HUMAN
AccessioniPrimary (citable) accession number: Q8IXK0
Secondary accession number(s): A1L4Q1
, A8KA40, D3DPR2, Q2TAL3, Q5T0C1, Q6NUJ6, Q6ZQR1, Q8N306, Q8TAG8, Q96BL4, Q9Y4Y7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

The hPRC-H complex purification reported by PubMed:12167701 probably presents a mixture of different PRC1-like complexes.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.