Q8IXI2 (MIRO1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial Rho GTPase 1
Short name=MIRO-1
Short name=hMiro-1
EC=3.6.5.-

Alternative name(s):
Rac-GTP-binding protein-like protein
Ras homolog gene family member T1

Gene names

Name:	RHOT1
Synonyms:	ARHT1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	618 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution. Ref.2 Ref.9
Subunit structure	Interacts with the kinesin-binding proteins TRAK1/OIP106 and TRAK2/GRIF1, forming a link between mitochondria and the trafficking apparatus of the microtubules. Ref.9
Subcellular location	Mitochondrion outer membrane; Single-pass type IV membrane protein. Note: Colocalizes with MGARP and RHOT2 at the mitochondria. Ref.2 Ref.10
Tissue specificity	Ubiquitously expressed. Expressed at high level in heart and skeletal muscle. Ref.2
Sequence similarities	Belongs to the mitochondrial Rho GTPase family. Contains 2 EF-hand domains. Contains 2 Miro domains.
Sequence caution	The sequence AAH41114.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH68463.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA91969.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB14185.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat Transmembrane Transmembrane helix
Ligand	Calcium GTP-binding Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC apoptotic process Inferred from mutant phenotype Ref.2. Source: UniProtKB cellular homeostasis Inferred from mutant phenotype Ref.2. Source: UniProtKB mitochondrion transport along microtubule Inferred from mutant phenotype Ref.9. Source: UniProtKB regulation of small GTPase mediated signal transduction Traceable author statement. Source: Reactome small GTPase mediated signal transduction Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome integral to mitochondrial outer membrane Inferred from direct assay Ref.2Ref.10. Source: UniProtKB plasma membrane Traceable author statement. Source: Reactome
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
KIF5C	O60282	2	EBI-1396430,EBI-717170
Kif5c	P56536	5	EBI-1396430,EBI-994504	From a different organism.
PARK2	O60260	3	EBI-1396430,EBI-716346
PINK1	Q9BXM7	3	EBI-1396430,EBI-2846068
TRAK1	Q9UPV9	3	EBI-1396430,EBI-1105048

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8IXI2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8IXI2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     580-580: P → PEDHYRDRLSRDMGHTDRIENLRKIWVFLKTAL

Isoform 3 (identifier: Q8IXI2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     580-580: P → PEDHYRDRLSRDMGHTDRIENLRKIWVFLKTAFHARLRCMCTCNRCTFCICQNFLNSDLLQSVKNKIFTAVLNR

Isoform 4 (identifier: Q8IXI2-4)

The sequence of this isoform differs from the canonical sequence as follows:
     581-618: Missing.

Note: No experimental confirmation available.

Isoform 5 (identifier: Q8IXI2-5)

The sequence of this isoform differs from the canonical sequence as follows:
     582-618: VTQADLKSST...AMYKALLKQR → ARLRCMCTCN...TAVLNRIISA

Note: No experimental confirmation available.

Isoform 6 (identifier: Q8IXI2-6)

The sequence of this isoform differs from the canonical sequence as follows:
     224-247: QALEDVKNVVRKHISDGVADSGLT → RFGFEQVLVLLFLQFWALLCTKHY
     248-618: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 618	618	Mitochondrial Rho GTPase 1		PRO_0000239313
Regions
	Topological domain	1 – 592	592	Mitochondrial intermembrane Potential
	Transmembrane	593 – 615	23	Helical; Anchor for type IV membrane protein; Potential
	Topological domain	616 – 618	3	Cytoplasmic Potential
	Domain	1 – 177	177	Miro 1
	Domain	184 – 219	36	EF-hand 1
	Domain	304 – 339	36	EF-hand 2
	Domain	412 – 561	150	Miro 2
	Nucleotide binding	11 – 18	8	GTP 1
	Nucleotide binding	57 – 61	5	GTP 1 Potential
	Nucleotide binding	118 – 121	4	GTP 1 Potential
	Calcium binding	197 – 208	12	1 Probable
	Calcium binding	317 – 328	12	2 Probable
	Nucleotide binding	425 – 432	8	GTP 2
	Nucleotide binding	463 – 467	5	GTP 2 Potential
	Nucleotide binding	527 – 530	4	GTP 2 Potential
Natural variations
	Alternative sequence	224 – 247	24	QALED…DSGLT → RFGFEQVLVLLFLQFWALLC TKHY in isoform 6.		VSP_019153
	Alternative sequence	248 – 618	371	Missing in isoform 6.		VSP_019154
	Alternative sequence	580	1	P → PEDHYRDRLSRDMGHTDRIE NLRKIWVFLKTAL in isoform 2.		VSP_019155
	Alternative sequence	580	1	P → PEDHYRDRLSRDMGHTDRIE NLRKIWVFLKTAFHARLRCM CTCNRCTFCICQNFLNSDLL QSVKNKIFTAVLNR in isoform 3.		VSP_019156
	Alternative sequence	581 – 618	38	Missing in isoform 4.		VSP_019157
	Alternative sequence	582 – 618	37	VTQAD…LLKQR → ARLRCMCTCNRCTFCICQNF LNSDLLQSVKNKIFTAVLNR IISA in isoform 5.		VSP_019158
Experimental info
	Mutagenesis	13	1	P → V: Causes constitutive activation inducing an aggregation of the mitochondrial network. Ref.2 Ref.8 Ref.9
	Mutagenesis	18	1	T → N: Causes constitutive inactivation. Ref.2 Ref.9
	Mutagenesis	208	1	E → K: Abolishes the formation of thread-like mitochondria. Ref.9
	Mutagenesis	328	1	E → K: Abolishes the formation of thread-like mitochondria. Ref.9
	Mutagenesis	427	1	K → V: No effect. Ref.9
	Mutagenesis	432	1	S → N: No effect. Ref.9
	Sequence conflict	75	1	A → V in AAM15734. Ref.3
	Sequence conflict	246	1	L → F in AAH60781. Ref.7
	Sequence conflict	246	1	L → F in BAA91969. Ref.5
	Sequence conflict	331	1	D → G in AAH60781. Ref.7
	Sequence conflict	587	1	L → P in CAD56956. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 13, 2006. Version 2. Checksum: FE47AC3A4AF6F6C2	FASTA	618	70,784
	10 20 30 40 50 60 MKKDVRILLV GEPRVGKTSL IMSLVSEEFP EEVPPRAEEI TIPADVTPER VPTHIVDYSE 70 80 90 100 110 120 AEQSDEQLHQ EISQANVICI VYAVNNKHSI DKVTSRWIPL INERTDKDSR LPLILVGNKS 130 140 150 160 170 180 DLVEYSSMET ILPIMNQYTE IETCVECSAK NLKNISELFY YAQKAVLHPT GPLYCPEEKE 190 200 210 220 230 240 MKPACIKALT RIFKISDQDN DGTLNDAELN FFQRICFNTP LAPQALEDVK NVVRKHISDG 250 260 270 280 290 300 VADSGLTLKG FLFLHTLFIQ RGRHETTWTV LRRFGYDDDL DLTPEYLFPL LKIPPDCTTE 310 320 330 340 350 360 LNHHAYLFLQ STFDKHDLDR DCALSPDELK DLFKVFPYIP WGPDVNNTVC TNERGWITYQ 370 380 390 400 410 420 GFLSQWTLTT YLDVQRCLEY LGYLGYSILT EQESQASAVT VTRDKKIDLQ KKQTQRNVFR 430 440 450 460 470 480 CNVIGVKNCG KSGVLQALLG RNLMRQKKIR EDHKSYYAIN TVYVYGQEKY LLLHDISESE 490 500 510 520 530 540 FLTEAEIICD VVCLVYDVSN PKSFEYCARI FKQHFMDSRI PCLIVAAKSD LHEVKQEYSI 550 560 570 580 590 600 SPTDFCRKHK MPPPQAFTCN TADAPSKDIF VKLTTMAMYP HVTQADLKSS TFWLRASFGA 610 TVFAVLGFAM YKALLKQR
	Isoform 2 [UniParc]. Checksum: 9E2A92E79F9160E1	FASTA	650	74,752
	10 20 30 40 50 60 MKKDVRILLV GEPRVGKTSL IMSLVSEEFP EEVPPRAEEI TIPADVTPER VPTHIVDYSE 70 80 90 100 110 120 AEQSDEQLHQ EISQANVICI VYAVNNKHSI DKVTSRWIPL INERTDKDSR LPLILVGNKS 130 140 150 160 170 180 DLVEYSSMET ILPIMNQYTE IETCVECSAK NLKNISELFY YAQKAVLHPT GPLYCPEEKE 190 200 210 220 230 240 MKPACIKALT RIFKISDQDN DGTLNDAELN FFQRICFNTP LAPQALEDVK NVVRKHISDG 250 260 270 280 290 300 VADSGLTLKG FLFLHTLFIQ RGRHETTWTV LRRFGYDDDL DLTPEYLFPL LKIPPDCTTE 310 320 330 340 350 360 LNHHAYLFLQ STFDKHDLDR DCALSPDELK DLFKVFPYIP WGPDVNNTVC TNERGWITYQ 370 380 390 400 410 420 GFLSQWTLTT YLDVQRCLEY LGYLGYSILT EQESQASAVT VTRDKKIDLQ KKQTQRNVFR 430 440 450 460 470 480 CNVIGVKNCG KSGVLQALLG RNLMRQKKIR EDHKSYYAIN TVYVYGQEKY LLLHDISESE 490 500 510 520 530 540 FLTEAEIICD VVCLVYDVSN PKSFEYCARI FKQHFMDSRI PCLIVAAKSD LHEVKQEYSI 550 560 570 580 590 600 SPTDFCRKHK MPPPQAFTCN TADAPSKDIF VKLTTMAMYP EDHYRDRLSR DMGHTDRIEN 610 620 630 640 650 LRKIWVFLKT ALHVTQADLK SSTFWLRASF GATVFAVLGF AMYKALLKQR
	Isoform 3 [UniParc]. Checksum: 478FF7C2F8DD1986	FASTA	691	79,547
	10 20 30 40 50 60 MKKDVRILLV GEPRVGKTSL IMSLVSEEFP EEVPPRAEEI TIPADVTPER VPTHIVDYSE 70 80 90 100 110 120 AEQSDEQLHQ EISQANVICI VYAVNNKHSI DKVTSRWIPL INERTDKDSR LPLILVGNKS 130 140 150 160 170 180 DLVEYSSMET ILPIMNQYTE IETCVECSAK NLKNISELFY YAQKAVLHPT GPLYCPEEKE 190 200 210 220 230 240 MKPACIKALT RIFKISDQDN DGTLNDAELN FFQRICFNTP LAPQALEDVK NVVRKHISDG 250 260 270 280 290 300 VADSGLTLKG FLFLHTLFIQ RGRHETTWTV LRRFGYDDDL DLTPEYLFPL LKIPPDCTTE 310 320 330 340 350 360 LNHHAYLFLQ STFDKHDLDR DCALSPDELK DLFKVFPYIP WGPDVNNTVC TNERGWITYQ 370 380 390 400 410 420 GFLSQWTLTT YLDVQRCLEY LGYLGYSILT EQESQASAVT VTRDKKIDLQ KKQTQRNVFR 430 440 450 460 470 480 CNVIGVKNCG KSGVLQALLG RNLMRQKKIR EDHKSYYAIN TVYVYGQEKY LLLHDISESE 490 500 510 520 530 540 FLTEAEIICD VVCLVYDVSN PKSFEYCARI FKQHFMDSRI PCLIVAAKSD LHEVKQEYSI 550 560 570 580 590 600 SPTDFCRKHK MPPPQAFTCN TADAPSKDIF VKLTTMAMYP EDHYRDRLSR DMGHTDRIEN 610 620 630 640 650 660 LRKIWVFLKT AFHARLRCMC TCNRCTFCIC QNFLNSDLLQ SVKNKIFTAV LNRHVTQADL 670 680 690 KSSTFWLRAS FGATVFAVLG FAMYKALLKQ R
	Isoform 4 [UniParc]. Checksum: EC42DCCD95811BC3	FASTA	580	66,541
	10 20 30 40 50 60 MKKDVRILLV GEPRVGKTSL IMSLVSEEFP EEVPPRAEEI TIPADVTPER VPTHIVDYSE 70 80 90 100 110 120 AEQSDEQLHQ EISQANVICI VYAVNNKHSI DKVTSRWIPL INERTDKDSR LPLILVGNKS 130 140 150 160 170 180 DLVEYSSMET ILPIMNQYTE IETCVECSAK NLKNISELFY YAQKAVLHPT GPLYCPEEKE 190 200 210 220 230 240 MKPACIKALT RIFKISDQDN DGTLNDAELN FFQRICFNTP LAPQALEDVK NVVRKHISDG 250 260 270 280 290 300 VADSGLTLKG FLFLHTLFIQ RGRHETTWTV LRRFGYDDDL DLTPEYLFPL LKIPPDCTTE 310 320 330 340 350 360 LNHHAYLFLQ STFDKHDLDR DCALSPDELK DLFKVFPYIP WGPDVNNTVC TNERGWITYQ 370 380 390 400 410 420 GFLSQWTLTT YLDVQRCLEY LGYLGYSILT EQESQASAVT VTRDKKIDLQ KKQTQRNVFR 430 440 450 460 470 480 CNVIGVKNCG KSGVLQALLG RNLMRQKKIR EDHKSYYAIN TVYVYGQEKY LLLHDISESE 490 500 510 520 530 540 FLTEAEIICD VVCLVYDVSN PKSFEYCARI FKQHFMDSRI PCLIVAAKSD LHEVKQEYSI 550 560 570 580 SPTDFCRKHK MPPPQAFTCN TADAPSKDIF VKLTTMAMYP
	Isoform 5 [UniParc]. Checksum: 3968C61EE9945024	FASTA	625	71,687
	10 20 30 40 50 60 MKKDVRILLV GEPRVGKTSL IMSLVSEEFP EEVPPRAEEI TIPADVTPER VPTHIVDYSE 70 80 90 100 110 120 AEQSDEQLHQ EISQANVICI VYAVNNKHSI DKVTSRWIPL INERTDKDSR LPLILVGNKS 130 140 150 160 170 180 DLVEYSSMET ILPIMNQYTE IETCVECSAK NLKNISELFY YAQKAVLHPT GPLYCPEEKE 190 200 210 220 230 240 MKPACIKALT RIFKISDQDN DGTLNDAELN FFQRICFNTP LAPQALEDVK NVVRKHISDG 250 260 270 280 290 300 VADSGLTLKG FLFLHTLFIQ RGRHETTWTV LRRFGYDDDL DLTPEYLFPL LKIPPDCTTE 310 320 330 340 350 360 LNHHAYLFLQ STFDKHDLDR DCALSPDELK DLFKVFPYIP WGPDVNNTVC TNERGWITYQ 370 380 390 400 410 420 GFLSQWTLTT YLDVQRCLEY LGYLGYSILT EQESQASAVT VTRDKKIDLQ KKQTQRNVFR 430 440 450 460 470 480 CNVIGVKNCG KSGVLQALLG RNLMRQKKIR EDHKSYYAIN TVYVYGQEKY LLLHDISESE 490 500 510 520 530 540 FLTEAEIICD VVCLVYDVSN PKSFEYCARI FKQHFMDSRI PCLIVAAKSD LHEVKQEYSI 550 560 570 580 590 600 SPTDFCRKHK MPPPQAFTCN TADAPSKDIF VKLTTMAMYP HARLRCMCTC NRCTFCICQN 610 620 FLNSDLLQSV KNKIFTAVLN RIISA
	Isoform 6 [UniParc]. Checksum: 3802160B22935376	FASTA	247	28,265
	10 20 30 40 50 60 MKKDVRILLV GEPRVGKTSL IMSLVSEEFP EEVPPRAEEI TIPADVTPER VPTHIVDYSE 70 80 90 100 110 120 AEQSDEQLHQ EISQANVICI VYAVNNKHSI DKVTSRWIPL INERTDKDSR LPLILVGNKS 130 140 150 160 170 180 DLVEYSSMET ILPIMNQYTE IETCVECSAK NLKNISELFY YAQKAVLHPT GPLYCPEEKE 190 200 210 220 230 240 MKPACIKALT RIFKISDQDN DGTLNDAELN FFQRICFNTP LAPRFGFEQV LVLLFLQFWA LLCTKHY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete physical map and gene content of the human NF1 tumor suppressor region in human and mouse." Jenne D.E., Tinschert S., Dorschner M.O., Hameister H., Stephens K., Kehrer-Sawatzki H. Genes Chromosomes Cancer 37:111-120(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[2]	"Atypical Rho GTPases have roles in mitochondrial homeostasis and apoptosis." Fransson A., Ruusala A., Aspenstroem P. J. Biol. Chem. 278:6495-6502(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF PRO-13 AND THR-18.
[3]	"Cloning a novel Rac-GTP binding protein-like gene from human fetal brain." Zeng L., Xie Y., Mao Y. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Fetal brain.
[4]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Uterus.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 134-618 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 157-618 (ISOFORM 3). Tissue: Amygdala, Placenta and Teratocarcinoma.
[6]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 5), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 18-618 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 90-550 (ISOFORM 6). Tissue: Brain, Lung, Testis and Uterus.
[8]	"Rho GTPases have diverse effects on the organization of the actin filament system." Aspenstroem P., Fransson A., Saras J. Biochem. J. 377:327-337(2004) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF PRO-13.
[9]	"The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in mitochondrial trafficking." Fransson S., Ruusala A., Aspenstroem P. Biochem. Biophys. Res. Commun. 344:500-510(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH TRAK1 AND TRAK2, MUTAGENESIS OF PRO-13; THR-18; GLU-208; GLU-328; LYS-427 AND SER-432.
[10]	"HUMMR, a hypoxia- and HIF-1alpha-inducible protein, alters mitochondrial distribution and transport." Li Y., Lim S., Hoffman D., Aspenstrom P., Federoff H.J., Rempe D.A. J. Cell Biol. 185:1065-1081(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ496730 mRNA. Translation: CAD43139.1. AJ517412 mRNA. Translation: CAD56956.1. AY094972 mRNA. Translation: AAM15734.1. AL136929 mRNA. Translation: CAB66863.1. AK001902 mRNA. Translation: BAA91969.1. Different initiation. AK022695 mRNA. Translation: BAB14185.1. Different initiation. AK294407 mRNA. Translation: BAG57659.1. AC116407 Genomic DNA. No translation available. BC015698 mRNA. Translation: AAH15698.1. BC041114 mRNA. Translation: AAH41114.1. Different initiation. BC051818 mRNA. Translation: AAH51818.1. BC060781 mRNA. Translation: AAH60781.2. BC068463 mRNA. Translation: AAH68463.1. Different initiation. BC125104 mRNA. Translation: AAI25105.1. BC125105 mRNA. Translation: AAI25106.1.
IPI	IPI00217536. IPI00432452. IPI00651681. IPI00759627. IPI00759714. IPI00759775.
RefSeq	NP_001028738.1. NM_001033566.1. NP_001028740.1. NM_001033568.1. NP_060777.3. NM_018307.3.
UniGene	Hs.655325.
3D structure databases
ProteinModelPortal	Q8IXI2.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8IXI2. 8 interactions.
PTM databases
PhosphoSite	Q8IXI2.
Polymorphism databases
DMDM	108860796.
Proteomic databases
PaxDb	Q8IXI2.
PRIDE	Q8IXI2.
Protocols and materials databases
DNASU	55288.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000333942; ENSP00000334724; ENSG00000126858. ENST00000358365; ENSP00000351132; ENSG00000126858. ENST00000394692; ENSP00000378184; ENSG00000126858. ENST00000581031; ENSP00000464094; ENSG00000126858. ENST00000581094; ENSP00000462669; ENSG00000126858.
GeneID	55288.
KEGG	hsa:55288.
UCSC	uc002hgv.3. human. uc002hgw.3. human. uc002hgy.3. human. uc002hha.3. human.
Organism-specific databases
CTD	55288.
GeneCards	GC17P030470.
HGNC	HGNC:21168. RHOT1.
HPA	HPA010687.
MIM	613888. gene.
neXtProt	NX_Q8IXI2.
PharmGKB	PA134906318.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1100.
HOVERGEN	HBG079778.
KO	K07870.
OMA	YDVSNPK.
OrthoDB	EOG4SXNC2.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q8IXI2.
Bgee	Q8IXI2.
CleanEx	HS_RHOT1.
Genevestigator	Q8IXI2.
GermOnline	ENSG00000126858. Homo sapiens.
Family and domain databases
Gene3D	1.10.238.10. 2 hits.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR013566. EF_hand_assoc_1. IPR013567. EF_hand_assoc_2. IPR002048. EF_hand_dom. IPR020860. MIRO. IPR013684. MIRO-like. IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR021181. Small_GTPase_Miro. [Graphical view]
PANTHER	PTHR24072:SF69. PTHR24072:SF69. 1 hit.
Pfam	PF08355. EF_assoc_1. 1 hit. PF08356. EF_assoc_2. 1 hit. PF13405. EF_hand_4. 1 hit. PF08477. Miro. 1 hit. PF00071. Ras. 1 hit. [Graphical view]
PIRSF	PIRSF037488. Mt_Rho_GTPase. 1 hit.
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00054. EFh. 2 hits. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 2 hits. PS51423. MIRO. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RHOT1. human.
GenomeRNAi	55288.
NextBio	59462.
SOURCE	Search...

Entry information

Entry name

MIRO1_HUMAN

Accession

Primary (citable) accession number: Q8IXI2
Secondary accession number(s): A4FVB6 , A6NFV0, B4DG48, Q6NUR3, Q6P9F8, Q6PJG1, Q6YMW8, Q86UB0, Q8IW28, Q8IXJ7, Q9H067, Q9H9N8, Q9NUZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	June 13, 2006
Last modified:	May 1, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.