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Protein

Sperm acrosome membrane-associated protein 3

Gene

SPACA3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane. The processed form has no detectable bacteriolytic activity in vitro.1 Publication

GO - Molecular functioni

  • lysozyme activity Source: UniProtKB

GO - Biological processi

  • cell wall macromolecule catabolic process Source: UniProtKB
  • defense response to Gram-negative bacterium Source: GO_Central
  • defense response to Gram-positive bacterium Source: UniProtKB
  • monocyte activation Source: UniProtKB
  • peptidoglycan catabolic process Source: UniProtKB
  • positive regulation of macrophage activation Source: UniProtKB
  • positive regulation of phagocytosis Source: UniProtKB
  • response to virus Source: UniProtKB
  • sperm-egg recognition Source: Ensembl
Complete GO annotation...

Protein family/group databases

CAZyiGH22. Glycoside Hydrolase Family 22.

Names & Taxonomyi

Protein namesi
Recommended name:
Sperm acrosome membrane-associated protein 3
Alternative name(s):
Cancer/testis antigen 54
Short name:
CT54
Lysozyme-like acrosomal sperm-specific secretory protein ALLP-17
Lysozyme-like protein 3
Sperm lysozyme-like protein 1
Sperm protein reactive with antisperm antibodies
Short name:
Sperm protein reactive with ASA
Cleaved into the following 2 chains:
Gene namesi
Name:SPACA3
Synonyms:LYC3, LYZL3, SLLP1, SPRASA
ORF Names:UNQ424/PRO862
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:16260. SPACA3.

Subcellular locationi

Isoform 1 :

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 63CytoplasmicSequence analysisAdd BLAST63
Transmembranei64 – 84Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini85 – 215ExtracellularSequence analysisAdd BLAST131

GO - Cellular componenti

  • acrosomal membrane Source: UniProtKB-SubCell
  • extracellular region Source: UniProtKB
  • extracellular space Source: GO_Central
  • integral component of membrane Source: UniProtKB-KW
  • lysosome Source: UniProtKB
  • secretory granule Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi124912.
OpenTargetsiENSG00000141316.
PharmGKBiPA38101.

Polymorphism and mutation databases

BioMutaiSPACA3.
DMDMi74723659.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002562191 – 215Sperm acrosome membrane-associated protein 3, membrane formAdd BLAST215
ChainiPRO_000025622088 – 215Sperm acrosome membrane-associated protein 3, processed formAdd BLAST128

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi93 ↔ 213PROSITE-ProRule annotation
Disulfide bondi117 ↔ 201PROSITE-ProRule annotation
Disulfide bondi151 ↔ 166PROSITE-ProRule annotation
Disulfide bondi162 ↔ 180PROSITE-ProRule annotation

Post-translational modificationi

The processed form derives from the membrane form by proteolytic processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei87 – 88Cleavage; to produce processed formCurated2

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ8IXA5.
PeptideAtlasiQ8IXA5.
PRIDEiQ8IXA5.

PTM databases

iPTMnetiQ8IXA5.
PhosphoSitePlusiQ8IXA5.

Expressioni

Tissue specificityi

The processed form is expressed in sperm (at protein level). Expressed in testis, epididymis and placenta.2 Publications

Gene expression databases

BgeeiENSG00000141316.
CleanExiHS_SPACA3.
ExpressionAtlasiQ8IXA5. baseline and differential.
GenevisibleiQ8IXA5. HS.

Organism-specific databases

HPAiHPA023633.

Interactioni

Subunit structurei

Interacts with ASTL.By similarity

Protein-protein interaction databases

BioGridi125897. 6 interactors.
IntActiQ8IXA5. 1 interactor.
STRINGi9606.ENSP00000269053.

Structurei

3D structure databases

ProteinModelPortaliQ8IXA5.
SMRiQ8IXA5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 22 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IG3E. Eukaryota.
ENOG41118PT. LUCA.
GeneTreeiENSGT00550000074398.
HOGENOMiHOG000037357.
HOVERGENiHBG052297.
InParanoidiQ8IXA5.
OMAiQGLGYWE.
OrthoDBiEOG091G0R9V.
PhylomeDBiQ8IXA5.
TreeFamiTF324882.

Family and domain databases

CDDicd00119. LYZ1. 1 hit.
InterProiIPR001916. Glyco_hydro_22.
IPR019799. Glyco_hydro_22_CS.
IPR000974. Glyco_hydro_22_lys.
IPR023346. Lysozyme-like_dom.
IPR030058. LYZL3.
[Graphical view]
PANTHERiPTHR11407:SF25. PTHR11407:SF25. 1 hit.
PfamiPF00062. Lys. 1 hit.
[Graphical view]
PRINTSiPR00137. LYSOZYME.
PR00135. LYZLACT.
SMARTiSM00263. LYZ1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiPS00128. LACTALBUMIN_LYSOZYME_1. 1 hit.
PS51348. LACTALBUMIN_LYSOZYME_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8IXA5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVSALRGAPL IRVHSSPVSS PSVSGPRRLV SCLSSQSSAL SQSGGGSTSA
60 70 80 90 100
AGIEARSRAL RRRWCPAGIM LLALVCLLSC LLPSSEAKLY GRCELARVLH
110 120 130 140 150
DFGLDGYRGY SLADWVCLAY FTSGFNAAAL DYEADGSTNN GIFQINSRRW
160 170 180 190 200
CSNLTPNVPN VCRMYCSDLL NPNLKDTVIC AMKITQEPQG LGYWEAWRHH
210
CQGKDLTEWV DGCDF
Length:215
Mass (Da):23,431
Last modified:March 1, 2003 - v1
Checksum:i39988565D592BCC8
GO
Isoform 2 (identifier: Q8IXA5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-69: Missing.

Show »
Length:146
Mass (Da):16,430
Checksum:i58CE171F2581158C
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02888580C → Y.Corresponds to variant rs16967845dbSNPEnsembl.1
Natural variantiVAR_028886100H → R.Corresponds to variant rs28963dbSNPEnsembl.1
Natural variantiVAR_050008128A → T.Corresponds to variant rs35420663dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0213291 – 69Missing in isoform 2. 1 PublicationAdd BLAST69

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216311 mRNA. Translation: AAK01478.1.
AF099029 Genomic DNA. Translation: AAP97222.1.
AY358653 mRNA. Translation: AAQ89016.1.
BC100886 mRNA. Translation: AAI00887.1.
BC100887 mRNA. Translation: AAI00888.1.
CCDSiCCDS11275.1. [Q8IXA5-1]
RefSeqiNP_001304155.1. NM_001317226.1.
NP_776246.1. NM_173847.4. [Q8IXA5-1]
UniGeneiHs.434112.

Genome annotation databases

EnsembliENST00000269053; ENSP00000269053; ENSG00000141316. [Q8IXA5-1]
ENST00000580599; ENSP00000463386; ENSG00000141316. [Q8IXA5-2]
GeneIDi124912.
KEGGihsa:124912.
UCSCiuc002hhs.2. human. [Q8IXA5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216311 mRNA. Translation: AAK01478.1.
AF099029 Genomic DNA. Translation: AAP97222.1.
AY358653 mRNA. Translation: AAQ89016.1.
BC100886 mRNA. Translation: AAI00887.1.
BC100887 mRNA. Translation: AAI00888.1.
CCDSiCCDS11275.1. [Q8IXA5-1]
RefSeqiNP_001304155.1. NM_001317226.1.
NP_776246.1. NM_173847.4. [Q8IXA5-1]
UniGeneiHs.434112.

3D structure databases

ProteinModelPortaliQ8IXA5.
SMRiQ8IXA5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125897. 6 interactors.
IntActiQ8IXA5. 1 interactor.
STRINGi9606.ENSP00000269053.

Protein family/group databases

CAZyiGH22. Glycoside Hydrolase Family 22.

PTM databases

iPTMnetiQ8IXA5.
PhosphoSitePlusiQ8IXA5.

Polymorphism and mutation databases

BioMutaiSPACA3.
DMDMi74723659.

Proteomic databases

PaxDbiQ8IXA5.
PeptideAtlasiQ8IXA5.
PRIDEiQ8IXA5.

Protocols and materials databases

DNASUi124912.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000269053; ENSP00000269053; ENSG00000141316. [Q8IXA5-1]
ENST00000580599; ENSP00000463386; ENSG00000141316. [Q8IXA5-2]
GeneIDi124912.
KEGGihsa:124912.
UCSCiuc002hhs.2. human. [Q8IXA5-1]

Organism-specific databases

CTDi124912.
DisGeNETi124912.
GeneCardsiSPACA3.
H-InvDBHIX0039076.
HGNCiHGNC:16260. SPACA3.
HPAiHPA023633.
MIMi612749. gene.
neXtProtiNX_Q8IXA5.
OpenTargetsiENSG00000141316.
PharmGKBiPA38101.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IG3E. Eukaryota.
ENOG41118PT. LUCA.
GeneTreeiENSGT00550000074398.
HOGENOMiHOG000037357.
HOVERGENiHBG052297.
InParanoidiQ8IXA5.
OMAiQGLGYWE.
OrthoDBiEOG091G0R9V.
PhylomeDBiQ8IXA5.
TreeFamiTF324882.

Miscellaneous databases

ChiTaRSiSPACA3. human.
GeneWikiiSPACA3.
GenomeRNAii124912.
PROiQ8IXA5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000141316.
CleanExiHS_SPACA3.
ExpressionAtlasiQ8IXA5. baseline and differential.
GenevisibleiQ8IXA5. HS.

Family and domain databases

CDDicd00119. LYZ1. 1 hit.
InterProiIPR001916. Glyco_hydro_22.
IPR019799. Glyco_hydro_22_CS.
IPR000974. Glyco_hydro_22_lys.
IPR023346. Lysozyme-like_dom.
IPR030058. LYZL3.
[Graphical view]
PANTHERiPTHR11407:SF25. PTHR11407:SF25. 1 hit.
PfamiPF00062. Lys. 1 hit.
[Graphical view]
PRINTSiPR00137. LYSOZYME.
PR00135. LYZLACT.
SMARTiSM00263. LYZ1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiPS00128. LACTALBUMIN_LYSOZYME_1. 1 hit.
PS51348. LACTALBUMIN_LYSOZYME_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSACA3_HUMAN
AccessioniPrimary (citable) accession number: Q8IXA5
Secondary accession number(s): Q7Z4Y5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Although it belongs to the glycosyl hydrolase 22 family, Thr-122 and Asn-139 are present instead of the conserved Glu and Asp which are active site residues. It is therefore expected that this protein lacks hydrolase activity.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.