Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8IX07 (FOG1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger protein ZFPM1
Alternative name(s):
Friend of GATA protein 1
Short name=FOG-1
Short name=Friend of GATA 1
Zinc finger protein 89A
Zinc finger protein multitype 1
Gene names
Name:ZFPM1
Synonyms:FOG1, ZFN89A
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1006 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription regulator that plays an essential role in erythroid and megakaryocytic cell differentiation. Essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family GATA1, GATA2 and GATA3. Such heterodimer can both activate or repress transcriptional activity, depending on the cell and promoter context. The heterodimer formed with GATA proteins is essential to activate expression of genes such as NFE2, ITGA2B, alpha- and beta-globin, while it represses expression of KLF1. May be involved in regulation of some genes in gonads. May also be involved in cardiac development, in a non-redundant way with ZFPM2/FOG2 By similarity.

Subunit structure

Interacts with corepressor CTBP2; this interaction is however not essential for corepressor activity By similarity. Interacts with the N-terminal zinc-finger of GATA1, GATA2 and probably GATA3. Ref.1

Subcellular location

Nucleus By similarity.

Tissue specificity

Mainly expressed in hematopoietic tissues. Also expressed in adult cerebellum, stomach, lymph node, liver and pancreas. Expressed in fetal heart, liver and spleen. Ref.1

Domain

The CCHC-type zinc fingers 1, 5, 6 and 9 directly bind to GATA-type zinc fingers. The Tyr residue adjacent to the last Cys of the CCHC-type zinc finger is essential for the interaction with GATA-type zinc fingers By similarity.

Sequence similarities

Belongs to the FOG (Friend of GATA) family.

Contains 4 C2H2-type zinc fingers.

Contains 5 C2HC-type zinc fingers.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityPolymorphism
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionActivator
Repressor
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processT-helper cell lineage commitment

Inferred by curator PubMed 21646796. Source: BHF-UCL

atrial septum morphogenesis

Inferred from sequence or structural similarity. Source: BHF-UCL

atrioventricular valve morphogenesis

Inferred from sequence or structural similarity. Source: BHF-UCL

blood coagulation

Traceable author statement. Source: Reactome

cardiac muscle tissue morphogenesis

Inferred from sequence or structural similarity. Source: BHF-UCL

definitive erythrocyte differentiation

Inferred from electronic annotation. Source: Ensembl

embryonic hemopoiesis

Inferred from sequence or structural similarity. Source: BHF-UCL

erythrocyte differentiation

Inferred from sequence or structural similarity. Source: BHF-UCL

granulocyte differentiation

Inferred from electronic annotation. Source: Ensembl

megakaryocyte development

Inferred from electronic annotation. Source: Ensembl

megakaryocyte differentiation

Inferred from sequence or structural similarity. Source: BHF-UCL

mitral valve formation

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of fat cell differentiation

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of interleukin-4 biosynthetic process

Inferred from direct assay PubMed 21646796. Source: BHF-UCL

negative regulation of mast cell differentiation

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of protein binding

Inferred from sequence or structural similarity. Source: BHF-UCL

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 15920471. Source: GOC

outflow tract morphogenesis

Inferred from sequence or structural similarity. Source: BHF-UCL

platelet formation

Inferred from genetic interaction PubMed 11675338. Source: BHF-UCL

positive regulation of interferon-gamma biosynthetic process

Inferred from direct assay PubMed 21646796. Source: BHF-UCL

primitive erythrocyte differentiation

Inferred from electronic annotation. Source: Ensembl

regulation of chemokine production

Inferred from electronic annotation. Source: Ensembl

regulation of definitive erythrocyte differentiation

Inferred from direct assay PubMed 15920471. Source: BHF-UCL

transcriptional activation by promoter-enhancer looping

Inferred from sequence or structural similarity. Source: BHF-UCL

tricuspid valve formation

Inferred from sequence or structural similarity. Source: BHF-UCL

ventricular septum morphogenesis

Inferred from sequence or structural similarity. Source: BHF-UCL

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: Ensembl

nucleoplasm

Traceable author statement. Source: Reactome

transcription factor complex

Inferred from direct assay PubMed 15920471. Source: BHF-UCL

transcriptional repressor complex

Inferred from direct assay PubMed 15920471. Source: BHF-UCL

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription

Inferred from direct assay PubMed 15920471. Source: BHF-UCL

RNA polymerase II transcription factor binding

Inferred from physical interaction PubMed 11675338PubMed 15920471. Source: BHF-UCL

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

transcription factor binding

Inferred from physical interaction Ref.1. Source: BHF-UCL

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10061006Zinc finger protein ZFPM1
PRO_0000221041

Regions

Zinc finger241 – 26424C2HC-type 1
Zinc finger290 – 31425C2H2-type 1
Zinc finger320 – 34223C2H2-type 2
Zinc finger348 – 37124C2H2-type 3
Zinc finger577 – 699123C2HC-type 2
Zinc finger683 – 70523C2HC-type 3
Zinc finger817 – 83923C2HC-type 4
Zinc finger854 – 87724C2H2-type 4
Zinc finger974 – 100027C2HC-type 5
Region330 – 34112Interaction with TACC3 By similarity
Region794 – 8007Interaction with CTBP2 By similarity

Amino acid modifications

Modified residue7861Phosphoserine Ref.6
Modified residue9011Phosphoserine Ref.8
Modified residue9091Phosphoserine Ref.8
Modified residue9351Phosphoserine By similarity

Natural variations

Natural variant701G → A.
Corresponds to variant rs34916016 [ dbSNP | Ensembl ].
VAR_057491

Experimental info

Sequence conflict221R → G in AAN45858. Ref.1
Sequence conflict444 – 4474EPLA → AP in AAN45858. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8IX07 [UniParc].

Last modified May 18, 2010. Version 2.
Checksum: E9C2363503A64898

FASTA1,006104,888
        10         20         30         40         50         60 
MSRRKQSNPR QIKRSLGDME AREEVQLVGA SHMEQKATAP EAPSPPSADV NSPPPLPPPT 

        70         80         90        100        110        120 
SPGGPKELEG QEPEPRPTEE EPGSPWSGPD ELEPVVQDGQ RRIRARLSLA TGLSWGPFHG 

       130        140        150        160        170        180 
SVQTRASSPR QAEPSPALTL LLVDEACWLR TLPQALTEAE ANTEIHRKDD ALWCRVTKPV 

       190        200        210        220        230        240 
PAGGLLSVLL TAEPHSTPGH PVKKEPAEPT CPAPAHDLQL LPQQAGMASI LATAVINKDV 

       250        260        270        280        290        300 
FPCKDCGIWY RSERNLQAHL LYYCASRQGT GSPAAAATDE KPKETYPNER VCPFPQCRKS 

       310        320        330        340        350        360 
CPSASSLEIH MRSHSGERPF VCLICLSAFT TKANCERHLK VHTDTLSGVC HSCGFISTTR 

       370        380        390        400        410        420 
DILYSHLVTN HMVCQPGSKG EIYSPGAGHP ATKLPPDSLG SFQQQHTALQ GPLASADLGL 

       430        440        450        460        470        480 
APTPSPGLDR KALAEATNGE ARAEPLAQNG GSSEPPAAPR SIKVEAVEEP EAAPILGPGE 

       490        500        510        520        530        540 
PGPQAPSRTP SPRSPAPARV KAELSSPTPG SSPVPGELGL AGALFLPQYV FGPDAAPPAS 

       550        560        570        580        590        600 
EILAKMSELV HSRLQQGAGA GAGGAQTGLF PGAPKGATCF ECEITFSNVN NYYVHKRLYC 

       610        620        630        640        650        660 
SGRRAPEDAP AARRPKAPPG PARAPPGQPA EPDAPRSSPG PGAREEGAGG AATPEDGAGG 

       670        680        690        700        710        720 
RGSEGSQSPG SSVDDAEDDP SRTLCEACNI RFSRHETYTV HKRYYCASRH DPPPRRPAAP 

       730        740        750        760        770        780 
PGPPGPAAPP APSPAAPVRT RRRRKLYELH AAGAPPPPPP GHAPAPESPR PGSGSGSGPG 

       790        800        810        820        830        840 
LAPARSPGPA ADGPIDLSKK PRRPLPGAPA PALADYHECT ACRVSFHSLE AYLAHKKYSC 

       850        860        870        880        890        900 
PAAPPPGALG LPAAACPYCP PNGPVRGDLL EHFRLAHGLL LGAPLAGPGV EARTPADRGP 

       910        920        930        940        950        960 
SPAPAPAASP QPGSRGPRDG LGPEPQEPPP GPPPSPAAAP EAVPPPPAPP SYSDKGVQTP 

       970        980        990       1000 
SKGTPAPLPN GNHRYCRLCN IKFSSLSTFI AHKKYYCSSH AAEHVK 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of the GATA1 cofactor human FOG1 and assessment of its binding to GATA1 proteins carrying D218 substitutions."
Freson K., Thys C., Wittewrongel C., Vermylen J., Hoylaerts M.F., Van Geet C.
Hum. Genet. 112:42-49(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH GATA1 AND GATA2.
Tissue: Megakaryocyte.
[2]"The sequence and analysis of duplication-rich human chromosome 16."
Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. expand/collapse author list , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Toward a global characterization of the phosphoproteome in prostate cancer cells: identification of phosphoproteins in the LNCaP cell line."
Giorgianni F., Zhao Y., Desiderio D.M., Beranova-Giorgianni S.
Electrophoresis 28:2027-2034(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Prostate cancer.
[5]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic kidney.
[6]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-786, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-901 AND SER-909, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF488691 mRNA. Translation: AAN45858.1.
AC116552 Genomic DNA. No translation available.
AC135049 Genomic DNA. No translation available.
CH471184 Genomic DNA. Translation: EAW66806.1.
RefSeqNP_722520.2. NM_153813.2.
UniGeneHs.632218.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2XU7X-ray1.90C/D1-15[»]
ProteinModelPortalQ8IX07.
SMRQ8IX07. Positions 241-439, 971-1000.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid127806. 8 interactions.
DIPDIP-48415N.
IntActQ8IX07. 3 interactions.
STRING9606.ENSP00000326630.

PTM databases

PhosphoSiteQ8IX07.

Polymorphism databases

DMDM296434508.

Proteomic databases

PaxDbQ8IX07.
PRIDEQ8IX07.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000319555; ENSP00000326630; ENSG00000179588.
GeneID161882.
KEGGhsa:161882.
UCSCuc002fkv.3. human.

Organism-specific databases

CTD161882.
GeneCardsGC16P088519.
HGNCHGNC:19762. ZFPM1.
HPAHPA046603.
MIM601950. gene.
neXtProtNX_Q8IX07.
PharmGKBPA134920282.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG146797.
HOGENOMHOG000112626.
HOVERGENHBG101018.
InParanoidQ8IX07.
KOK17441.
OMAKLYELHA.
OrthoDBEOG74TWXR.
PhylomeDBQ8IX07.
TreeFamTF331342.

Enzyme and pathway databases

ReactomeREACT_604. Hemostasis.

Gene expression databases

BgeeQ8IX07.
CleanExHS_ZFPM1.
GenevestigatorQ8IX07.

Family and domain databases

Gene3D3.30.160.60. 2 hits.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 9 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSZFPM1. human.
GeneWikiZFPM1.
GenomeRNAi161882.
NextBio88126.
PROQ8IX07.
SOURCESearch...

Entry information

Entry nameFOG1_HUMAN
AccessionPrimary (citable) accession number: Q8IX07
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: May 18, 2010
Last modified: April 16, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM