Q8IX07 (FOG1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein ZFPM1

Alternative name(s):
Friend of GATA protein 1
Short name=FOG-1
Short name=Friend of GATA 1
Zinc finger protein 89A
Zinc finger protein multitype 1

Gene names

Name:	ZFPM1
Synonyms:	FOG1, ZFN89A

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1006 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription regulator that plays an essential role in erythroid and megakaryocytic cell differentiation. Essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family GATA1, GATA2 and GATA3. Such heterodimer can both activate or repress transcriptional activity, depending on the cell and promoter context. The heterodimer formed with GATA proteins is essential to activate expression of genes such as NFE2, ITGA2B, alpha- and beta-globin, while it represses expression of KLF1. May be involved in regulation of some genes in gonads. May also be involved in cardiac development, in a non-redundant way with ZFPM2/FOG2 By similarity.
Subunit structure	Interacts with corepressor CTBP2; this interaction is however not essential for corepressor activity By similarity. Interacts with the N-terminal zinc-finger of GATA1, GATA2 and probably GATA3. Ref.1
Subcellular location	Nucleus By similarity.
Tissue specificity	Mainly expressed in hematopoietic tissues. Also expressed in adult cerebellum, stomach, lymph node, liver and pancreas. Expressed in fetal heart, liver and spleen. Ref.1
Domain	The CCHC-type zinc fingers 1, 5, 6 and 9 directly bind to GATA-type zinc fingers. The Tyr residue adjacent to the last Cys of the CCHC-type zinc finger is essential for the interaction with GATA-type zinc fingers By similarity.
Sequence similarities	Belongs to the FOG (Friend of GATA) family. Contains 4 C2H2-type zinc fingers. Contains 5 C2HC-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator Repressor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	T-helper cell lineage commitment Inferred by curator PubMed 21646796. Source: BHF-UCL atrial septum morphogenesis Inferred from sequence or structural similarity. Source: BHF-UCL atrioventricular valve morphogenesis Inferred from sequence or structural similarity. Source: BHF-UCL blood coagulation Traceable author statement. Source: Reactome cardiac muscle tissue morphogenesis Inferred from sequence or structural similarity. Source: BHF-UCL definitive erythrocyte differentiation Inferred from electronic annotation. Source: Compara embryonic hemopoiesis Inferred from sequence or structural similarity. Source: BHF-UCL erythrocyte differentiation Inferred from sequence or structural similarity. Source: BHF-UCL granulocyte differentiation Inferred from electronic annotation. Source: Compara megakaryocyte development Inferred from electronic annotation. Source: Compara megakaryocyte differentiation Inferred from sequence or structural similarity. Source: BHF-UCL mitral valve formation Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of fat cell differentiation Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of interleukin-4 biosynthetic process Inferred from direct assay PubMed 21646796. Source: BHF-UCL negative regulation of mast cell differentiation Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of protein binding Inferred from sequence or structural similarity. Source: BHF-UCL outflow tract morphogenesis Inferred from sequence or structural similarity. Source: BHF-UCL platelet formation Inferred from genetic interaction PubMed 11675338. Source: BHF-UCL positive regulation of interferon-gamma biosynthetic process Inferred from direct assay PubMed 21646796. Source: BHF-UCL primitive erythrocyte differentiation Inferred from electronic annotation. Source: Compara regulation of chemokine production Inferred from electronic annotation. Source: Compara regulation of definitive erythrocyte differentiation Inferred from direct assay PubMed 15920471. Source: BHF-UCL transcriptional activation by promoter-enhancer looping Inferred from sequence or structural similarity. Source: BHF-UCL tricuspid valve formation Inferred from sequence or structural similarity. Source: BHF-UCL ventricular septum morphogenesis Inferred from sequence or structural similarity. Source: BHF-UCL
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara transcription factor complex Inferred from direct assay PubMed 15920471. Source: BHF-UCL transcriptional repressor complex Inferred from direct assay PubMed 15920471. Source: BHF-UCL
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Inferred from direct assay PubMed 15920471. Source: BHF-UCL zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GSK3B	P49841	2	EBI-3942619,EBI-373586
RBBP4	Q09028	4	EBI-3942619,EBI-620823

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1006	1006	Zinc finger protein ZFPM1		PRO_0000221041
Regions
	Zinc finger	241 – 264	24	C2HC-type 1
	Zinc finger	290 – 314	25	C2H2-type 1
	Zinc finger	320 – 342	23	C2H2-type 2
	Zinc finger	348 – 371	24	C2H2-type 3
	Zinc finger	577 – 699	123	C2HC-type 2
	Zinc finger	683 – 705	23	C2HC-type 3
	Zinc finger	817 – 839	23	C2HC-type 4
	Zinc finger	854 – 877	24	C2H2-type 4
	Zinc finger	974 – 1000	27	C2HC-type 5
	Region	330 – 341	12	Interaction with TACC3 By similarity
	Region	794 – 800	7	Interaction with CTBP2 By similarity
Amino acid modifications
	Modified residue	491	1	Phosphoserine By similarity
	Modified residue	494	1	Phosphoserine By similarity
	Modified residue	638	1	Phosphoserine By similarity
	Modified residue	653	1	Phosphothreonine By similarity
	Modified residue	786	1	Phosphoserine Ref.6
	Modified residue	901	1	Phosphoserine Ref.7
	Modified residue	909	1	Phosphoserine Ref.7
	Modified residue	935	1	Phosphoserine By similarity
Natural variations
	Natural variant	70	1	G → A. Corresponds to variant rs34916016 [ dbSNP | Ensembl ].		VAR_057491
Experimental info
	Sequence conflict	22	1	R → G in AAN45858. Ref.1
	Sequence conflict	444 – 447	4	EPLA → AP in AAN45858. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8IX07 [UniParc]. Last modified May 18, 2010. Version 2. Checksum: E9C2363503A64898	FASTA	1,006	104,888
	10 20 30 40 50 60 MSRRKQSNPR QIKRSLGDME AREEVQLVGA SHMEQKATAP EAPSPPSADV NSPPPLPPPT 70 80 90 100 110 120 SPGGPKELEG QEPEPRPTEE EPGSPWSGPD ELEPVVQDGQ RRIRARLSLA TGLSWGPFHG 130 140 150 160 170 180 SVQTRASSPR QAEPSPALTL LLVDEACWLR TLPQALTEAE ANTEIHRKDD ALWCRVTKPV 190 200 210 220 230 240 PAGGLLSVLL TAEPHSTPGH PVKKEPAEPT CPAPAHDLQL LPQQAGMASI LATAVINKDV 250 260 270 280 290 300 FPCKDCGIWY RSERNLQAHL LYYCASRQGT GSPAAAATDE KPKETYPNER VCPFPQCRKS 310 320 330 340 350 360 CPSASSLEIH MRSHSGERPF VCLICLSAFT TKANCERHLK VHTDTLSGVC HSCGFISTTR 370 380 390 400 410 420 DILYSHLVTN HMVCQPGSKG EIYSPGAGHP ATKLPPDSLG SFQQQHTALQ GPLASADLGL 430 440 450 460 470 480 APTPSPGLDR KALAEATNGE ARAEPLAQNG GSSEPPAAPR SIKVEAVEEP EAAPILGPGE 490 500 510 520 530 540 PGPQAPSRTP SPRSPAPARV KAELSSPTPG SSPVPGELGL AGALFLPQYV FGPDAAPPAS 550 560 570 580 590 600 EILAKMSELV HSRLQQGAGA GAGGAQTGLF PGAPKGATCF ECEITFSNVN NYYVHKRLYC 610 620 630 640 650 660 SGRRAPEDAP AARRPKAPPG PARAPPGQPA EPDAPRSSPG PGAREEGAGG AATPEDGAGG 670 680 690 700 710 720 RGSEGSQSPG SSVDDAEDDP SRTLCEACNI RFSRHETYTV HKRYYCASRH DPPPRRPAAP 730 740 750 760 770 780 PGPPGPAAPP APSPAAPVRT RRRRKLYELH AAGAPPPPPP GHAPAPESPR PGSGSGSGPG 790 800 810 820 830 840 LAPARSPGPA ADGPIDLSKK PRRPLPGAPA PALADYHECT ACRVSFHSLE AYLAHKKYSC 850 860 870 880 890 900 PAAPPPGALG LPAAACPYCP PNGPVRGDLL EHFRLAHGLL LGAPLAGPGV EARTPADRGP 910 920 930 940 950 960 SPAPAPAASP QPGSRGPRDG LGPEPQEPPP GPPPSPAAAP EAVPPPPAPP SYSDKGVQTP 970 980 990 1000 SKGTPAPLPN GNHRYCRLCN IKFSSLSTFI AHKKYYCSSH AAEHVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of the GATA1 cofactor human FOG1 and assessment of its binding to GATA1 proteins carrying D218 substitutions." Freson K., Thys C., Wittewrongel C., Vermylen J., Hoylaerts M.F., Van Geet C. Hum. Genet. 112:42-49(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH GATA1 AND GATA2. Tissue: Megakaryocyte.
[2]	"The sequence and analysis of duplication-rich human chromosome 16." Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A. Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Toward a global characterization of the phosphoproteome in prostate cancer cells: identification of phosphoproteins in the LNCaP cell line." Giorgianni F., Zhao Y., Desiderio D.M., Beranova-Giorgianni S. Electrophoresis 28:2027-2034(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Prostate cancer.
[5]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Embryonic kidney.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-786, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-901 AND SER-909, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF488691 mRNA. Translation: AAN45858.1.
AC116552 Genomic DNA. No translation available.
AC135049 Genomic DNA. No translation available.
CH471184 Genomic DNA. Translation: EAW66806.1.

IPI

IPI00217346.

RefSeq

NP_722520.2. NM_153813.2.

UniGene

Hs.632218.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2XU7	X-ray	1.90	C/D	1-15	[»]

ProteinModelPortal

Q8IX07.

SMR

Q8IX07. Positions 234-370, 847-875, 964-1003.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-48415N.

IntAct

Q8IX07. 3 interactions.

STRING

9606.ENSP00000326630.

PTM databases

PhosphoSite

Q8IX07.

Polymorphism databases

DMDM

296434508.

Proteomic databases

PaxDb

Q8IX07.

PRIDE

Q8IX07.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000319555; ENSP00000326630; ENSG00000179588.

GeneID

161882.

KEGG

hsa:161882.

UCSC

uc002fkv.3. human.

Organism-specific databases

CTD

161882.

GeneCards

GC16P088519.

HGNC

HGNC:19762. ZFPM1.

HPA

HPA046603.

MIM

601950. gene.

neXtProt

NX_Q8IX07.

PharmGKB

PA134920282.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG146797.

HOGENOM

HOG000112626.

HOVERGEN

HBG101018.

InParanoid

Q8IX07.

OMA

CTACSIS.

OrthoDB

EOG4N5VZN.

PhylomeDB

Q8IX07.

Enzyme and pathway databases

Pathway_Interaction_DB

hdac_classi_pathway. Signaling events mediated by HDAC Class I.

Reactome

REACT_604. Hemostasis.

Gene expression databases

Bgee

Q8IX07.

CleanEx

HS_ZFPM1.

Genevestigator

Q8IX07.

GermOnline

ENSG00000179588. Homo sapiens.

Family and domain databases

Gene3D

3.30.160.60. 2 hits.

InterPro

IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]

SMART

SM00355. ZnF_C2H2. 9 hits.
[Graphical view]

PROSITE

PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

ZFPM1. human.

GenomeRNAi

161882.

NextBio

88126.

SOURCE

Search...

Entry information

Entry name

FOG1_HUMAN

Accession

Primary (citable) accession number: Q8IX07

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.