Q8IX05 (CD302_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CD302 antigen

Alternative name(s):
C-type lectin BIMLEC
C-type lectin domain family 13 member A
DEC205-associated C-type lectin 1
Type I transmembrane C-type lectin receptor DCL-1
CD_antigen=CD302

Gene names

Name:	CD302
Synonyms:	CLEC13A, DCL1, KIAA0022

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Potential multifunctional C-type lectin receptor that may play roles in endocytosis and phagocytosis as well as in cell adhesion and migration. Ref.7
Subcellular location	Membrane; Single-pass type I membrane protein Potential. Cell projection › filopodium. Cytoplasm › cell cortex. Cell projection › microvillus. Note: Colocalizes with F-actin in filopodia, cellular cortex and microvilli of the apical cell surface. Ref.7
Tissue specificity	Expressed at moderate levels in monocytes, myeloid blood dendritic cells and granulocytes and at low levels in plasmacytoid blood dendritic cells, monocyte-derived ma crophages and monocyte-derived dendritic cells, with no expression detected in T-lymphocytes, B-lymphocytes and natural killer cells (at protein level). Expressed widely in different tissues, with highest expression levels in liver, lung, peripheral blood leukocytes and spleen, and lowest levels in neuronal tissues, skeletal muscle and ovary. Isoform 2 and isoform 3 are expressed in malignant Hodgkin lymphoma cells called Hodgkin and Reed-Sternberg (HRS) cells. Ref.1 Ref.7
Developmental stage	Expressed at relatively high levels in fetal lung, liver, spleen and kidney with lower expression levels detected in heart, thymus and brain. Ref.7
Post-translational modification	May be heterogeneously N-glycosylated in some cell types. Ref.7
Miscellaneous	Isoform 2 and isoform 3 are produced in HRS cells by a transcriptional control mechanism which cotranscribe an mRNA containing LY75 and CD302 prior to generating the intergenically spliced mRNA to produce LY75/CD302 fusion proteins.
Sequence similarities	Contains 1 C-type lectin domain.

Ontologies

Keywords
Cellular component	Cell projection Cytoplasm Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal Transmembrane Transmembrane helix
Ligand	Lectin
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phagocytosis Inferred from direct assay Ref.7. Source: MGI
Cellular_component	cell cortex Inferred from direct assay Ref.7. Source: MGI filopodium Inferred from direct assay Ref.7. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microvillus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8IX05-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Produced by intergenic splicing of LY75 and CD302.

Isoform 4 (identifier: O60449-1)

The sequence of this isoform can be found in the external entry O60449.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform 5 (identifier: Q8IX05-2)

The sequence of this isoform differs from the canonical sequence as follows:
     99-156: Missing.
     157-157: I → V

Isoform 2 (identifier: O60449-2)

Also known as: Fusion protein variant V34-2;

The sequence of this isoform can be found in the external entry O60449.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by intergenic splicing of LY75 and CD302.

Isoform 3 (identifier: O60449-3)

Also known as: Fusion protein variant V33-2;

The sequence of this isoform can be found in the external entry O60449.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by intergenic splicing of LY75 and CD302.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 232	210	CD302 antigen		PRO_0000252332
Regions
	Topological domain	23 – 168	146	Extracellular Potential
	Transmembrane	169 – 189	21	Helical; Potential
	Topological domain	190 – 232	43	Cytoplasmic Potential
	Domain	32 – 152	121	C-type lectin
Amino acid modifications
	Glycosylation	109	1	N-linked (GlcNAc...) Potential
	Disulfide bond	128 ↔ 143		By similarity
Natural variations
	Alternative sequence	99 – 156	58	Missing in isoform 5.		VSP_044258
	Alternative sequence	157	1	I → V in isoform 5.		VSP_044259
	Natural variant	200	1	R → S. Corresponds to variant rs34068933 [ dbSNP | Ensembl ].		VAR_050101
Experimental info
	Sequence conflict	139	1	K → R in BAF83968. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 5C33C708345C3971	FASTA	232	26,183
	10 20 30 40 50 60 MLRAALPALL LPLLGLAAAA VADCPSSTWI QFQDSCYIFL QEAIKVESIE DVRNQCTDHG 70 80 90 100 110 120 ADMISIHNEE ENAFILDTLK KQWKGPDDIL LGMFYDTDDA SFKWFDNSNM TFDKWTDQDD 130 140 150 160 170 180 DEDLVDTCAF LHIKTGEWKK GNCEVSSVEG TLCKTAIPYK RKYLSDNHIL ISALVIASTV 190 200 210 220 230 ILTVLGAIIW FLYKKHSDSR FTTVFSTAPQ SPYNEDCVLV VGEENEYPVQ FD
	Isoform 4 [UniParc]. See O60449.		–	–
	Isoform 5 [UniParc]. Checksum: AF737C517D8B6931	FASTA	174	19,562
	10 20 30 40 50 60 MLRAALPALL LPLLGLAAAA VADCPSSTWI QFQDSCYIFL QEAIKVESIE DVRNQCTDHG 70 80 90 100 110 120 ADMISIHNEE ENAFILDTLK KQWKGPDDIL LGMFYDTDVP YKRKYLSDNH ILISALVIAS 130 140 150 160 170 TVILTVLGAI IWFLYKKHSD SRFTTVFSTA PQSPYNEDCV LVVGEENEYP VQFD
	Isoform 2 (Fusion protein variant V34-2) [UniParc]. See O60449.		–	–
	Isoform 3 (Fusion protein variant V33-2) [UniParc]. See O60449.		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Hodgkin's lymphoma cell lines express a fusion protein encoded by intergenically spliced mRNA for the multilectin receptor DEC-205 (CD205) and a novel C-type lectin receptor DCL-1." Kato M., Khan S., Gonzalez N., O'Neill B.P., McDonald K.J., Cooper B.J., Angel N.Z., Hart D.N.J. J. Biol. Chem. 278:34035-34041(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), TISSUE SPECIFICITY.
[2]	"Identification of a novel C-type lectin, BIMLEC, from BCG cell wall induced monocyte." Begum N.A. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 5). Tissue: Teratocarcinoma and Trachea.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1." Nomura N., Miyajima N., Sazuka T., Tanaka A., Kawarabayasi Y., Sato S., Nagase T., Seki N., Ishikawa K., Tabata S. DNA Res. 1:27-35(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 63-232 (ISOFORM 1). Tissue: Bone marrow.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 63-232 (ISOFORM 1). Tissue: Liver.
[7]	"The novel endocytic and phagocytic C-Type lectin receptor DCL-1/CD302 on macrophages is colocalized with F-actin, suggesting a role in cell adhesion and migration." Kato M., Khan S., d'Aniello E., McDonald K.J., Hart D.N. J. Immunol. 179:6052-6063(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, GLYCOSYLATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY314007 mRNA. Translation: AAP79900.1. AF499000 mRNA. Translation: AAN77050.1. AK291279 mRNA. Translation: BAF83968.1. AK304422 mRNA. Translation: BAG65251.1. AC009961 Genomic DNA. Translation: AAY14942.1. D14664 mRNA. Translation: BAA03498.1. BC020646 mRNA. Translation: AAH20646.1.
IPI	IPI00217343.
RefSeq	NP_001185692.1. NM_001198763.1. NP_055695.2. NM_014880.4.
UniGene	Hs.130014.
3D structure databases
ProteinModelPortal	Q8IX05.
ModBase	Search...
PTM databases
PhosphoSite	Q8IX05.
Polymorphism databases
DMDM	74750720.
Proteomic databases
PaxDb	Q8IX05.
PRIDE	Q8IX05.
Protocols and materials databases
DNASU	9936.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000259053; ENSP00000259053; ENSG00000241399. ENST00000429078; ENSP00000394301; ENSG00000241399.
GeneID	9936.
KEGG	hsa:9936.
UCSC	uc002uba.3. human.
Organism-specific databases
CTD	9936.
GeneCards	GC02M160625.
H-InvDB	HIX0002534. HIX0161882.
HGNC	HGNC:30843. CD302.
MIM	612246. gene.
neXtProt	NX_Q8IX05.
PharmGKB	PA142672141.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG288401.
HOGENOM	HOG000232049.
HOVERGEN	HBG080529.
KO	K06722.
OMA	EENEYAV.
OrthoDB	EOG4GXFNR.
PhylomeDB	Q8IX05.
Gene expression databases
ArrayExpress	Q8IX05.
Bgee	Q8IX05.
CleanEx	HS_CD302.
Genevestigator	Q8IX05.
GermOnline	ENSG00000136539. Homo sapiens.
Family and domain databases
Gene3D	3.10.100.10. 1 hit.
InterPro	IPR001304. C-type_lectin. IPR016186. C-type_lectin-like. IPR016187. C-type_lectin_fold. [Graphical view]
Pfam	PF00059. Lectin_C. 1 hit. [Graphical view]
SMART	SM00034. CLECT. 1 hit. [Graphical view]
SUPFAM	SSF56436. C-type_lectin_fold. 1 hit.
PROSITE	PS00615. C_TYPE_LECTIN_1. False negative. PS50041. C_TYPE_LECTIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	9936.
NextBio	37484.
SOURCE	Search...

Entry information

Entry name

CD302_HUMAN

Accession

Primary (citable) accession number: Q8IX05
Secondary accession number(s): A8K5G4, B4E2T9, Q15009

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.