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Q8IX05 (CD302_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CD302 antigen
Alternative name(s):
C-type lectin BIMLEC
C-type lectin domain family 13 member A
DEC205-associated C-type lectin 1
Type I transmembrane C-type lectin receptor DCL-1
CD_antigen=CD302
Gene names
Name:CD302
Synonyms:CLEC13A, DCL1, KIAA0022
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length232 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Potential multifunctional C-type lectin receptor that may play roles in endocytosis and phagocytosis as well as in cell adhesion and migration. Ref.7

Subcellular location

Membrane; Single-pass type I membrane protein Potential. Cell projectionfilopodium. Cytoplasmcell cortex. Cell projectionmicrovillus. Note: Colocalizes with F-actin in filopodia, cellular cortex and microvilli of the apical cell surface. Ref.7

Tissue specificity

Expressed at moderate levels in monocytes, myeloid blood dendritic cells and granulocytes and at low levels in plasmacytoid blood dendritic cells, monocyte-derived ma crophages and monocyte-derived dendritic cells, with no expression detected in T-lymphocytes, B-lymphocytes and natural killer cells (at protein level). Expressed widely in different tissues, with highest expression levels in liver, lung, peripheral blood leukocytes and spleen, and lowest levels in neuronal tissues, skeletal muscle and ovary. Isoform 2 and isoform 3 are expressed in malignant Hodgkin lymphoma cells called Hodgkin and Reed-Sternberg (HRS) cells. Ref.1 Ref.7

Developmental stage

Expressed at relatively high levels in fetal lung, liver, spleen and kidney with lower expression levels detected in heart, thymus and brain. Ref.7

Post-translational modification

May be heterogeneously N-glycosylated in some cell types. Ref.7

Miscellaneous

Isoform 2 and isoform 3 are produced in HRS cells by a transcriptional control mechanism which cotranscribe an mRNA containing LY75 and CD302 prior to generating the intergenically spliced mRNA to produce LY75/CD302 fusion proteins.

Sequence similarities

Contains 1 C-type lectin domain.

Ontologies

Keywords
   Cellular componentCell projection
Cytoplasm
Membrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSignal
Transmembrane
Transmembrane helix
   LigandLectin
   Molecular functionReceptor
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processphagocytosis

Inferred from direct assay Ref.7. Source: MGI

   Cellular_componentcell cortex

Inferred from direct assay Ref.7. Source: MGI

filopodium

Inferred from direct assay Ref.7. Source: MGI

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

microvillus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncarbohydrate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8IX05-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by intergenic splicing of LY75 and CD302.
Isoform 4 (identifier: O60449-1)

The sequence of this isoform can be found in the external entry O60449.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoform 5 (identifier: Q8IX05-2)

The sequence of this isoform differs from the canonical sequence as follows:
     99-156: Missing.
     157-157: I → V
Isoform 2 (identifier: O60449-2)

Also known as: Fusion protein variant V34-2;

The sequence of this isoform can be found in the external entry O60449.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by intergenic splicing of LY75 and CD302.
Isoform 3 (identifier: O60449-3)

Also known as: Fusion protein variant V33-2;

The sequence of this isoform can be found in the external entry O60449.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by intergenic splicing of LY75 and CD302.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 232210CD302 antigen
PRO_0000252332

Regions

Topological domain23 – 168146Extracellular Potential
Transmembrane169 – 18921Helical; Potential
Topological domain190 – 23243Cytoplasmic Potential
Domain32 – 152121C-type lectin

Amino acid modifications

Glycosylation1091N-linked (GlcNAc...) Potential
Disulfide bond128 ↔ 143 By similarity

Natural variations

Alternative sequence99 – 15658Missing in isoform 5.
VSP_044258
Alternative sequence1571I → V in isoform 5.
VSP_044259
Natural variant2001R → S.
Corresponds to variant rs34068933 [ dbSNP | Ensembl ].
VAR_050101

Experimental info

Sequence conflict1391K → R in BAF83968. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 5C33C708345C3971

FASTA23226,183
        10         20         30         40         50         60 
MLRAALPALL LPLLGLAAAA VADCPSSTWI QFQDSCYIFL QEAIKVESIE DVRNQCTDHG 

        70         80         90        100        110        120 
ADMISIHNEE ENAFILDTLK KQWKGPDDIL LGMFYDTDDA SFKWFDNSNM TFDKWTDQDD 

       130        140        150        160        170        180 
DEDLVDTCAF LHIKTGEWKK GNCEVSSVEG TLCKTAIPYK RKYLSDNHIL ISALVIASTV 

       190        200        210        220        230 
ILTVLGAIIW FLYKKHSDSR FTTVFSTAPQ SPYNEDCVLV VGEENEYPVQ FD 

« Hide

Isoform 4 [UniParc].

See O60449.

Isoform 5 [UniParc].

Checksum: AF737C517D8B6931
Show »

FASTA17419,562
Isoform 2 (Fusion protein variant V34-2) [UniParc].

See O60449.

Isoform 3 (Fusion protein variant V33-2) [UniParc].

See O60449.

References

« Hide 'large scale' references
[1]"Hodgkin's lymphoma cell lines express a fusion protein encoded by intergenically spliced mRNA for the multilectin receptor DEC-205 (CD205) and a novel C-type lectin receptor DCL-1."
Kato M., Khan S., Gonzalez N., O'Neill B.P., McDonald K.J., Cooper B.J., Angel N.Z., Hart D.N.J.
J. Biol. Chem. 278:34035-34041(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), TISSUE SPECIFICITY.
[2]"Identification of a novel C-type lectin, BIMLEC, from BCG cell wall induced monocyte."
Begum N.A.
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 5).
Tissue: Teratocarcinoma and Trachea.
[4]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1."
Nomura N., Miyajima N., Sazuka T., Tanaka A., Kawarabayasi Y., Sato S., Nagase T., Seki N., Ishikawa K., Tabata S.
DNA Res. 1:27-35(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 63-232 (ISOFORM 1).
Tissue: Bone marrow.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 63-232 (ISOFORM 1).
Tissue: Liver.
[7]"The novel endocytic and phagocytic C-Type lectin receptor DCL-1/CD302 on macrophages is colocalized with F-actin, suggesting a role in cell adhesion and migration."
Kato M., Khan S., d'Aniello E., McDonald K.J., Hart D.N.
J. Immunol. 179:6052-6063(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, GLYCOSYLATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY314007 mRNA. Translation: AAP79900.1.
AF499000 mRNA. Translation: AAN77050.1.
AK291279 mRNA. Translation: BAF83968.1.
AK304422 mRNA. Translation: BAG65251.1.
AC009961 Genomic DNA. Translation: AAY14942.1.
D14664 mRNA. Translation: BAA03498.1.
BC020646 mRNA. Translation: AAH20646.1.
CCDSCCDS33308.1. [Q8IX05-1]
CCDS56139.1. [Q8IX05-2]
RefSeqNP_001185692.1. NM_001198763.1. [Q8IX05-2]
NP_055695.2. NM_014880.4. [Q8IX05-1]
UniGeneHs.130014.
Hs.153563.

3D structure databases

ProteinModelPortalQ8IX05.
SMRQ8IX05. Positions 24-154.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115262. 3 interactions.

PTM databases

PhosphoSiteQ8IX05.

Polymorphism databases

DMDM74750720.

Proteomic databases

MaxQBQ8IX05.
PaxDbQ8IX05.
PRIDEQ8IX05.

Protocols and materials databases

DNASU9936.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000259053; ENSP00000259053; ENSG00000241399. [Q8IX05-1]
ENST00000429078; ENSP00000394301; ENSG00000241399. [Q8IX05-2]
GeneID9936.
KEGGhsa:9936.
UCSCuc002uba.3. human. [Q8IX05-1]
uc010zco.2. human. [Q8IX05-2]

Organism-specific databases

CTD9936.
GeneCardsGC02M160625.
H-InvDBHIX0002534.
HIX0161882.
HGNCHGNC:30843. CD302.
MIM612246. gene.
neXtProtNX_Q8IX05.
PharmGKBPA142672141.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG288401.
HOGENOMHOG000232049.
HOVERGENHBG080529.
KOK06722.
OMAWKNSEES.
OrthoDBEOG7FFMQR.
PhylomeDBQ8IX05.

Gene expression databases

BgeeQ8IX05.
CleanExHS_CD302.
GenevestigatorQ8IX05.

Family and domain databases

Gene3D3.10.100.10. 1 hit.
InterProIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
[Graphical view]
PfamPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMSSF56436. SSF56436. 1 hit.
PROSITEPS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiCD302.
GenomeRNAi9936.
NextBio37484.
PROQ8IX05.
SOURCESearch...

Entry information

Entry nameCD302_HUMAN
AccessionPrimary (citable) accession number: Q8IX05
Secondary accession number(s): A8K5G4, B4E2T9, Q15009
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries