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Q8IX04 (UEVLD_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2 variant 3

Short name=UEV-3
Alternative name(s):
EV and lactate/malate dehydrogenase domain-containing protein
Gene names
Name:UEVLD
Synonyms:UEV3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length471 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Possible negative regulator of polyubiquitination. Ref.1

Subunit structure

Homodimer.

Tissue specificity

Colon, colon carcinoma cell lines, normal cervical epithelium, carcinomas of the uterine cervix and peripheral blood leukocytes. Ref.1

Sequence similarities

In the N-terminal section; belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.

In the C-terminal section; belongs to the LDH/MDH superfamily.

Contains 1 UEV (ubiquitin E2 variant) domain.

Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.3 (identifier: Q8IX04-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.1 (identifier: Q8IX04-2)

The sequence of this isoform differs from the canonical sequence as follows:
     376-379: AMEL → DIMI
     380-471: Missing.
Isoform 3 Ref.6 (identifier: Q8IX04-3)

The sequence of this isoform differs from the canonical sequence as follows:
     43-64: Missing.
     376-379: AMEL → DIMI
     380-471: Missing.
Isoform 4 (identifier: Q8IX04-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-38: Missing.
     376-379: AMEL → DIMI
     380-471: Missing.
Isoform 5 (identifier: Q8IX04-5)

The sequence of this isoform differs from the canonical sequence as follows:
     205-215: GIADRLVLLDL → VESRSVTQAGV
     216-471: Missing.
Isoform 6 (identifier: Q8IX04-6)

The sequence of this isoform differs from the canonical sequence as follows:
     43-64: Missing.
Note: No experimental confirmation available.
Isoform 7 (identifier: Q8IX04-7)

The sequence of this isoform differs from the canonical sequence as follows:
     65-164: Missing.
     239-295: Missing.
     354-470: VLTWSGQEEV...SIHSLQQQLK → GI
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 471471Ubiquitin-conjugating enzyme E2 variant 3
PRO_0000278651

Regions

Domain2 – 145144UEV
Nucleotide binding191 – 21929NAD By similarity

Natural variations

Alternative sequence1 – 3838Missing in isoform 4.
VSP_023346
Alternative sequence43 – 6422Missing in isoform 3 and isoform 6. Ref.6
VSP_023347
Alternative sequence65 – 164100Missing in isoform 7.
VSP_045986
Alternative sequence205 – 21511GIADRLVLLDL → VESRSVTQAGV in isoform 5.
VSP_023348
Alternative sequence216 – 471256Missing in isoform 5.
VSP_023349
Alternative sequence239 – 29557Missing in isoform 7.
VSP_045987
Alternative sequence354 – 470117VLTWS…QQQLK → GI in isoform 7.
VSP_045988
Alternative sequence376 – 3794AMEL → DIMI in isoform 2, isoform 3 and isoform 4.
VSP_023350
Alternative sequence380 – 47192Missing in isoform 2, isoform 3 and isoform 4.
VSP_023351

Experimental info

Sequence conflict1481D → G in BAG59405. Ref.3
Sequence conflict2481V → A in BAG59405. Ref.3
Sequence conflict3661H → L in AAN32950. Ref.1
Sequence conflict3661H → L in BAA91985. Ref.3

Secondary structure

................................................... 471
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 20, 2007. Version 2.
Checksum: 2502659A566E7DDD

FASTA47152,264
        10         20         30         40         50         60 
MEFDCEGLRR LLGKYKFRDL TVEELRNVNV FFPHFKYSMD TYVFKDSSQK DLLNFTGTIP 

        70         80         90        100        110        120 
VMYQGNTYNI PIRFWILDSH PFAPPICFLK PTANMGILVG KHVDAQGRIY LPYLQNWSHP 

       130        140        150        160        170        180 
KSVIVGLIKE MIAKFQEELP MYSLSSSDEA RQVDLLAYIA KITEGVSDTN SKSWANHENK 

       190        200        210        220        230        240 
TVNKITVVGG GELGIACTLA ISAKGIADRL VLLDLSEGTK GATMDLEIFN LPNVEISKDL 

       250        260        270        280        290        300 
SASAHSKVVI FTVNSLGSSQ SYLDVVQSNV DMFRALVPAL GHYSQHSVLL VASQPVEIMT 

       310        320        330        340        350        360 
YVTWKLSTFP ANRVIGIGCN LDSQRLQYII TNVLKAQTSG KEVWVIGEQG EDKVLTWSGQ 

       370        380        390        400        410        420 
EEVVSHTSQV QLSNRAMELL RVKGQRSWSV GLSVADMVDS IVNNKKKVHS VSALAKGYYD 

       430        440        450        460        470 
INSEVFLSLP CILGTNGVSE VIKTTLKEDT VTEKLQSSAS SIHSLQQQLK L 

« Hide

Isoform 2 [UniParc].

Checksum: 100BCD1FA295FC60
Show »

FASTA37942,292
Isoform 3 [UniParc].

Checksum: B906E3358B342190
Show »

FASTA35739,778
Isoform 4 [UniParc].

Checksum: 7D5CD535296471FA
Show »

FASTA34137,611
Isoform 5 [UniParc].

Checksum: B82D7775A32AAB67
Show »

FASTA21524,233
Isoform 6 [UniParc].

Checksum: 2B5D772AEA5B1F51
Show »

FASTA44949,750
Isoform 7 [UniParc].

Checksum: A8FC6744B9B89325
Show »

FASTA19922,240

References

« Hide 'large scale' references
[1]"Identification and characterization of UEV3, a human cDNA with similarities to inactive E2 ubiquitin-conjugating enzymes."
Kloor M., Bork P., Duwe A., Klaes R., von Knebel Doeberitz M., Ridder R.
Biochim. Biophys. Acta 1579:219-224(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY.
Tissue: Placenta.
[2]"Full-length cDNA libraries and normalization."
Li W.B., Gruber C., Jessee J., Polayes D.
Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Neuroblastoma.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 4 AND 6).
Tissue: Placenta, Testis and Tongue.
[4]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3; 5 AND 7).
Tissue: Brain, Placenta and Testis.
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Structural investigation into the L-lactate dehydrogenase domain of human ubiquitin-conjugating enzyme E2-like isoform A."
Avvakumov G.V., Walker J.R., Xue S., Newman E.M., Finerty P.J. Jr., Butler-Cole C., Tempel W., Weigelt J., Sundstrom M., Arrowsmith C.H., Edwards A.M., Bochkarev A., Dhe-Paganon S.
Submitted (OCT-2006) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF503350 mRNA. Translation: AAN32950.1.
CR616778 mRNA. No translation available.
AK001930 mRNA. Translation: BAA91985.1.
AK296835 mRNA. Translation: BAG59405.1.
AK314521 mRNA. Translation: BAG37116.1.
AC027544 Genomic DNA. No translation available.
AC112694 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68380.1.
BC011011 mRNA. Translation: AAH11011.2.
BC064566 mRNA. Translation: AAH64566.1.
CD109744 mRNA. No translation available.
RefSeqNP_001035787.1. NM_001040697.2.
NP_001248311.1. NM_001261382.1.
NP_001248312.1. NM_001261383.1.
NP_001248313.1. NM_001261384.1.
NP_001248314.1. NM_001261385.1.
NP_001248315.1. NM_001261386.1.
NP_060784.3. NM_018314.4.
XP_005253058.1. XM_005253001.1.
UniGeneHs.407991.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2I6TX-ray2.10A/B171-471[»]
3DL2X-ray2.10A/B171-471[»]
ProteinModelPortalQ8IX04.
SMRQ8IX04. Positions 8-143, 181-471.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120580. 6 interactions.
IntActQ8IX04. 7 interactions.
STRING9606.ENSP00000379500.

PTM databases

PhosphoSiteQ8IX04.

Polymorphism databases

DMDM126253820.

Proteomic databases

PaxDbQ8IX04.
PRIDEQ8IX04.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000300038; ENSP00000300038; ENSG00000151116. [Q8IX04-5]
ENST00000320750; ENSP00000323353; ENSG00000151116. [Q8IX04-3]
ENST00000379387; ENSP00000368697; ENSG00000151116. [Q8IX04-6]
ENST00000396197; ENSP00000379500; ENSG00000151116. [Q8IX04-1]
ENST00000535484; ENSP00000441092; ENSG00000151116. [Q8IX04-4]
ENST00000541984; ENSP00000437538; ENSG00000151116. [Q8IX04-7]
ENST00000543987; ENSP00000442974; ENSG00000151116. [Q8IX04-2]
GeneID55293.
KEGGhsa:55293.
UCSCuc001mot.4. human. [Q8IX04-1]
uc001mou.4. human. [Q8IX04-2]
uc001mov.4. human. [Q8IX04-3]
uc010rdh.3. human. [Q8IX04-5]
uc031pzg.1. human. [Q8IX04-4]

Organism-specific databases

CTD55293.
GeneCardsGC11M018554.
HGNCHGNC:30866. UEVLD.
HPAHPA047134.
MIM610985. gene.
neXtProtNX_Q8IX04.
PharmGKBPA147357188.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0039.
HOGENOMHOG000213793.
HOVERGENHBG000462.
InParanoidQ8IX04.
OMALRVKGQR.
OrthoDBEOG7JT6WH.
PhylomeDBQ8IX04.
TreeFamTF314963.

Gene expression databases

ArrayExpressQ8IX04.
BgeeQ8IX04.
CleanExHS_UEVLD.
GenevestigatorQ8IX04.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
IPR016135. UBQ-conjugating_enzyme/RWD.
IPR008883. UEV_N.
[Graphical view]
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
PF05743. UEV. 1 hit.
[Graphical view]
PRINTSPR00086. LLDHDRGNASE.
SUPFAMSSF54495. SSF54495. 1 hit.
SSF56327. SSF56327. 1 hit.
PROSITEPS51322. UEV. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ8IX04.
GenomeRNAi55293.
NextBio59482.
PROQ8IX04.
SOURCESearch...

Entry information

Entry nameUEVLD_HUMAN
AccessionPrimary (citable) accession number: Q8IX04
Secondary accession number(s): B2RB69 expand/collapse secondary AC list , B4DL43, F5H6L6, H7BYD6, Q6P2F0, Q96FF5, Q9NUX7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: February 20, 2007
Last modified: April 16, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM