Q8IWZ8 (SUGP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SURP and G-patch domain-containing protein 1

Alternative name(s):
RNA-binding protein RBP
Splicing factor 4

Gene names

Name:	SUGP1
Synonyms:	SF4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	645 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in pre-mRNA splicing.
Subunit structure	Component of the spliceosome.
Subcellular location	Nucleus Probable.
Tissue specificity	Detected in adult testis and heart, and in adult and fetal brain, kidney and skeletal muscle. Ref.1
Sequence similarities	Contains 1 G-patch domain. Contains 2 SURP motif repeats.
Sequence caution	The sequence AAC08052.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAL68960.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAL68961.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nuclear mRNA splicing, via spliceosome Traceable author statement. Source: Reactome
Cellular component	nucleoplasm Traceable author statement. Source: Reactome spliceosomal complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8IWZ8-1)

Also known as: RNA-binding protein splice variant A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8IWZ8-2)

Also known as: RNA-binding protein splice variant B;

The sequence of this isoform differs from the canonical sequence as follows:
     181-222: SPPEGAETRK...DYKDNPAFAF → CLTKTVRPTP...SPHPLCRCGP
     223-645: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 645	645	SURP and G-patch domain-containing protein 1		PRO_0000097701
Regions
	Repeat	191 – 233	43	SURP motif 1
	Repeat	266 – 309	44	SURP motif 2
	Domain	562 – 609	48	G-patch
	Motif	380 – 386	7	Nuclear localization signal Potential
	Compositional bias	330 – 373	44	Pro-rich
	Compositional bias	441 – 480	40	Gln/Met-rich
Amino acid modifications
	Modified residue	409	1	Phosphoserine Ref.7 Ref.9
	Modified residue	411	1	Phosphoserine Ref.9
	Modified residue	414	1	Phosphoserine Ref.9
	Modified residue	485	1	Phosphoserine Ref.7 Ref.8
Natural variations
	Alternative sequence	181 – 222	42	SPPEG…PAFAF → CLTKTVRPTPPSSWCFVPGL GTPDSLLHLTLFSPHPLCRC GP in isoform 2.		VSP_013109
	Alternative sequence	223 – 645	423	Missing in isoform 2.		VSP_013110
	Natural variant	290	1	R → H. Corresponds to variant rs17751061 [ dbSNP | Ensembl ].		VAR_051339
	Natural variant	568	1	Q → H. Corresponds to variant rs1044980 [ dbSNP | Ensembl ].		VAR_051340
Experimental info
	Sequence conflict	497	1	E → K in AAN77123. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (RNA-binding protein splice variant A) [UniParc]. Last modified March 15, 2005. Version 2. Checksum: 27AF5ED41DFDDCB7	FASTA	645	72,471
	10 20 30 40 50 60 MSLKMDNRDV AGKANRWFGV APPKSGKMNM NILHQEELIA QKKREIEAKM EQKAKQNQVA 70 80 90 100 110 120 SPQPPHPGEI TNAHNSSCIS NKFANDGSFL QQFLKLQKAQ TSTDAPTSAP SAPPSTPTPS 130 140 150 160 170 180 AGKRSLLISR RTGLGLASLP GPVKSYSHAK QLPVAHRPSV FQSPDEDEEE DYEQWLEIKV 190 200 210 220 230 240 SPPEGAETRK VIEKLARFVA EGGPELEKVA MEDYKDNPAF AFLHDKNSRE FLYYRKKVAE 250 260 270 280 290 300 IRKEAQKSQA ASQKVSPPED EEVKNLAEKL ARFIADGGPE VETIALQNNR ENQAFSFLYE 310 320 330 340 350 360 PNSQGYKYYR QKLEEFRKAK ASSTGSFTAP DPGLKRKSPP EALSGSLPPA TTCPASSTPA 370 380 390 400 410 420 PTIIPAPAAP GKPASAATVK RKRKSRWGPE EDKVELPPAE LVQRDVDASP SPLSVQDLKG 430 440 450 460 470 480 LGYEKGKPVG LVGVTELSDA QKKQLKEQQE MQQMYDMIMQ HKRAMQDMQL LWEKAVQQHQ 490 500 510 520 530 540 HGYDSDEEVD SELGTWEHQL RRMEMDKTRE WAEQLTKMGR GKHFIGDFLP PDELEKFMET 550 560 570 580 590 600 FKALKEGREP DYSEYKEFKL TVENIGYQML MKMGWKEGEG LGSEGQGIKN PVNKGTTTVD 610 620 630 640 GAGFGIDRPA ELSKEDDEYE AFRKRMMLAY RFRPNPLNNP RRPYY
	Isoform 2 (RNA-binding protein splice variant B) [UniParc]. Checksum: 6CA11F2F5AC40E8E	FASTA	222	24,297
	10 20 30 40 50 60 MSLKMDNRDV AGKANRWFGV APPKSGKMNM NILHQEELIA QKKREIEAKM EQKAKQNQVA 70 80 90 100 110 120 SPQPPHPGEI TNAHNSSCIS NKFANDGSFL QQFLKLQKAQ TSTDAPTSAP SAPPSTPTPS 130 140 150 160 170 180 AGKRSLLISR RTGLGLASLP GPVKSYSHAK QLPVAHRPSV FQSPDEDEEE DYEQWLEIKV 190 200 210 220 CLTKTVRPTP PSSWCFVPGL GTPDSLLHLT LFSPHPLCRC GP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SF4 and SFRS14, two related putative splicing factors on human chromosome 19p13.11." Sampson N.D., Hewitt J.E. Gene 305:91-100(2003) [PubMed: 12594045] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]	"Novel isoforms of a human RNA-binding protein." Gu Y., Nguyen C.-T. Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-645 (ISOFORMS 1 AND 2).
[3]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-645 (ISOFORM 1). Tissue: PNS.
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 254-645. Tissue: Testis.
[6]	"Large-scale proteomic analysis of the human spliceosome." Rappsilber J., Ryder U., Lamond A.I., Mann M. Genome Res. 12:1231-1245(2002) [PubMed: 12176931] [Abstract] Cited for: MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH THE SPLICEOSOME.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-409 AND SER-485, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-485, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-409; SER-411 AND SER-414, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF521128 mRNA. Translation: AAN77123.1. AY072916 mRNA. Translation: AAL68960.1. Different initiation. AY072917 mRNA. Translation: AAL68961.1. Different initiation. AC004475 Genomic DNA. Translation: AAC08052.1. Sequence problems. BC063784 mRNA. Translation: AAH63784.1. AL137286 mRNA. Translation: CAB70678.1. AL713757 mRNA. Translation: CAD28528.1.
IPI	IPI00103419. IPI00337787.
PIR	T02299.
RefSeq	NP_757386.2. NM_172231.3.
UniGene	Hs.515274.
3D structure databases
ProteinModelPortal	Q8IWZ8.
SMR	Q8IWZ8. Positions 169-248, 254-323.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8IWZ8. 2 interactions.
MINT	MINT-3047702.
STRING	Q8IWZ8.
PTM databases
PhosphoSite	Q8IWZ8.
Polymorphism databases
DMDM	61216666.
Proteomic databases
PRIDE	Q8IWZ8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000247001; ENSP00000247001; ENSG00000105705.
GeneID	57794.
KEGG	hsa:57794.
UCSC	uc002nmh.1. human.
Organism-specific databases
CTD	57794.
GeneCards	GC19M019389.
H-InvDB	HIX0014937.
HGNC	HGNC:18643. SUGP1.
HPA	HPA004890.
MIM	607992. gene.
neXtProt	NX_Q8IWZ8.
PharmGKB	PA134988577. PA165394338.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG17044.
GeneTree	ENSGT00410000025695.
HOVERGEN	HBG079172.
InParanoid	Q8IWZ8.
OMA	EKVAMEN.
OrthoDB	EOG4VX24Z.
PhylomeDB	Q8IWZ8.
Enzyme and pathway databases
Reactome	REACT_1675. mRNA Processing. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	Q8IWZ8.
Bgee	Q8IWZ8.
CleanEx	HS_SF4.
Genevestigator	Q8IWZ8.
GermOnline	ENSG00000105705. Homo sapiens.
Family and domain databases
InterPro	IPR000467. G_patch. IPR000061. Surp. [Graphical view]
KO	K13096.
Pfam	PF01585. G-patch. 1 hit. PF01805. Surp. 2 hits. [Graphical view]
SMART	SM00443. G_patch. 1 hit. SM00648. SWAP. 2 hits. [Graphical view]
PROSITE	PS50174. G_PATCH. 1 hit. PS50128. SURP. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	64730.
SOURCE	Search...

Entry information

Entry name

SUGP1_HUMAN

Accession

Primary (citable) accession number: Q8IWZ8
Secondary accession number(s): O60378 , Q6P3X9, Q8TCQ4, Q8WWT4, Q8WWT5, Q9NTG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.