Q8IWV8 (UBR2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase UBR2
EC=6.3.2.-

Alternative name(s):
N-recognin-2
Ubiquitin-protein ligase E3-alpha-2
Ubiquitin-protein ligase E3-alpha-II

Gene names

Name:	UBR2
Synonyms:	C6orf133, KIAA0349

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1755 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Plays a critical role in chromatin inactivation and chromosome-wide transcriptional silencing during meiosis via ubiquitination of histone H2A. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth. Ref.1 Ref.10
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with UBE2B; promotes the UBE2B-H2A interaction and the ubiquitination of histone H2A by UBE2B and UBR2 By similarity. Interacts with RECQL4. Ref.6
Subcellular location	Nucleus By similarity. Note: Associated with chromatin during meiosis.
Tissue specificity	Broadly expressed, with highest levels in skeletal muscle, kidney and pancreas. Present in acinar cells of the pancreas (at protein level). Ref.1 Ref.7
Developmental stage	Expressed in fetal pancreas. Ref.7
Domain	The RING-H2 zinc finger is an atypical RING finger with a His ligand in place of the fourth Cys of the classical motif.
Sequence similarities	Belongs to the UBR1 family. Contains 1 RING-type zinc finger. Contains 1 UBR-type zinc finger.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to leucine Inferred from direct assay Ref.10. Source: UniProtKB chromatin silencing Inferred from sequence or structural similarity. Source: UniProtKB histone H2A ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB male meiosis I Inferred from electronic annotation. Source: Compara negative regulation of TOR signaling cascade Inferred from mutant phenotype Ref.10. Source: UniProtKB spermatogenesis Inferred from electronic annotation. Source: Compara ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: Compara
Cellular_component	chromatin Inferred from electronic annotation. Source: Compara nucleus Inferred from direct assay. Source: HPA plasma membrane Inferred from direct assay. Source: HPA ubiquitin ligase complex Inferred from electronic annotation. Source: Compara
Molecular_function	leucine binding Inferred from direct assay Ref.10. Source: UniProtKB ubiquitin-protein ligase activity Inferred from electronic annotation. Source: Compara zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
vif	P12504	3	EBI-1237260,EBI-779991	From a different organism.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8IWV8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8IWV8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     397-439: QQLQRDFMED...MLITEENLMS → ERLQSDYVTD...ISAGRSGSPL
     440-1755: Missing.

Isoform 3 (identifier: Q8IWV8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-496: Missing.
     497-514: RQKFLEGFDAFLELLKCM → MYAGNIPIYKTESRSRNE
     1022-1071: SSRDKDKAER...ELFQQTLELD → HNFRVQGTKT...MKTKNSFSRH
     1072-1755: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q8IWV8-4)

The sequence of this isoform differs from the canonical sequence as follows:
     397-426: QQLQRDFMEDDHERAVSVTALSVQFFTAPT → ERLQSDYVTDDHDREFSVADLSVQIFTVPS

Note: Derived from mouse cDNA data. No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1755

1755

E3 ubiquitin-protein ligase UBR2

PRO_0000056140

Regions

Zinc finger

97 – 168

72

UBR-type

Zinc finger

1108 – 1214

107

RING-type; atypical

Coiled coil

1019 – 1054

36

Potential

Natural variations

Alternative sequence

1 – 496

496

Missing in isoform 3.

VSP_015166

Alternative sequence

397 – 439

43

QQLQR…ENLMS → ERLQSDYVTDDHDREFSVAD LSVQIFTVPSLFSISAGRSG SPL in isoform 2.

VSP_015167

Alternative sequence

397 – 426

30

QQLQR…FTAPT → ERLQSDYVTDDHDREFSVAD LSVQIFTVPS in isoform 4.

VSP_017130

Alternative sequence

440 – 1755

1316

Missing in isoform 2.

VSP_015168

Alternative sequence

497 – 514

18

RQKFL…LLKCM → MYAGNIPIYKTESRSRNE in isoform 3.

VSP_015169

Alternative sequence

1022 – 1071

50

SSRDK…TLELD → HNFRVQGTKTKLRGREKQRL PDCAEKRSWLRCLKCSGILL MKTKNSFSRH in isoform 3.

VSP_015170

Alternative sequence

1072 – 1755

684

Missing in isoform 3.

VSP_015171

Natural variant

172

1

E → D.
Corresponds to variant rs6905054 [ dbSNP | Ensembl ].

VAR_052117

Natural variant

1095

1

A → P.
Corresponds to variant rs6917033 [ dbSNP | Ensembl ].

VAR_059816

Natural variant

1095

1

A → S.
Corresponds to variant rs6917033 [ dbSNP | Ensembl ].

VAR_059817

Natural variant

1095

1

A → T. Ref.5
Corresponds to variant rs6917033 [ dbSNP | Ensembl ].

VAR_023283

Experimental info

Sequence conflict

864

1

K → R in BAC86295. Ref.2

Secondary structure

1

.

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1755

Helix Strand Turn

Details...

Beta strand

108 – 112

5

Turn

113 – 115

3

Beta strand

116 – 118

3

Helix

125 – 129

5

Helix

132 – 135

4

Beta strand

138 – 142

5

Turn

154 – 156

3

Beta strand

157 – 159

3

Turn

164 – 166

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 04B14FCB13E21808	FASTA	1,755	200,538
	10 20 30 40 50 60 MASELEPEVQ AIDRSLLECS AEEIAGKWLQ ATDLTREVYQ HLAHYVPKIY CRGPNPFPQK 70 80 90 100 110 120 EDMLAQHVLL GPMEWYLCGE DPAFGFPKLE QANKPSHLCG RVFKVGEPTY SCRDCAVDPT 130 140 150 160 170 180 CVLCMECFLG SIHRDHRYRM TTSGGGGFCD CGDTEAWKEG PYCQKHELNT SEIEEEEDPL 190 200 210 220 230 240 VHLSEDVIAR TYNIFAITFR YAVEILTWEK ESELPADLEM VEKSDTYYCM LFNDEVHTYE 250 260 270 280 290 300 QVIYTLQKAV NCTQKEAIGF ATTVDRDGRR SVRYGDFQYC EQAKSVIVRN TSRQTKPLKV 310 320 330 340 350 360 QVMHSSIVAH QNFGLKLLSW LGSIIGYSDG LRRILCQVGL QEGPDGENSS LVDRLMLSDS 370 380 390 400 410 420 KLWKGARSVY HQLFMSSLLM DLKYKKLFAV RFAKNYQQLQ RDFMEDDHER AVSVTALSVQ 430 440 450 460 470 480 FFTAPTLARM LITEENLMSI IIKTFMDHLR HRDAQGRFQF ERYTALQAFK FRRVQSLILD 490 500 510 520 530 540 LKYVLISKPT EWSDELRQKF LEGFDAFLEL LKCMQGMDPI TRQVGQHIEM EPEWEAAFTL 550 560 570 580 590 600 QMKLTHVISM MQDWCASDEK VLIEAYKKCL AVLMQCHGGY TDGEQPITLS ICGHSVETIR 610 620 630 640 650 660 YCVSQEKVSI HLPVSRLLAG LHVLLSKSEV AYKFPELLPL SELSPPMLIE HPLRCLVLCA 670 680 690 700 710 720 QVHAGMWRRN GFSLVNQIYY YHNVKCRREM FDKDVVMLQT GVSMMDPNHF LMIMLSRFEL 730 740 750 760 770 780 YQIFSTPDYG KRFSSEITHK DVVQQNNTLI EEMLYLIIML VGERFSPGVG QVNATDEIKR 790 800 810 820 830 840 EIIHQLSIKP MAHSELVKSL PEDENKETGM ESVIEAVAHF KKPGLTGRGM YELKPECAKE 850 860 870 880 890 900 FNLYFYHFSR AEQSKAEEAQ RKLKRQNRED TALPPPVLPP FCPLFASLVN ILQSDVMLCI 910 920 930 940 950 960 MGTILQWAVE HNGYAWSESM LQRVLHLIGM ALQEEKQHLE NVTEEHVVTF TFTQKISKPG 970 980 990 1000 1010 1020 EAPKNSPSIL AMLETLQNAP YLEVHKDMIR WILKTFNAVK KMRESSPTSP VAETEGTIME 1030 1040 1050 1060 1070 1080 ESSRDKDKAE RKRKAEIARL RREKIMAQMS EMQRHFIDEN KELFQQTLEL DASTSAVLDH 1090 1100 1110 1120 1130 1140 SPVASDMTLT ALGPAQTQVP EQRQFVTCIL CQEEQEVKVE SRAMVLAAFV QRSTVLSKNR 1150 1160 1170 1180 1190 1200 SKFIQDPEKY DPLFMHPDLS CGTHTSSCGH IMHAHCWQRY FDSVQAKEQR RQQRLRLHTS 1210 1220 1230 1240 1250 1260 YDVENGEFLC PLCECLSNTV IPLLLPPRNI FNNRLNFSDQ PNLTQWIRTI SQQIKALQFL 1270 1280 1290 1300 1310 1320 RKEESTPNNA STKNSENVDE LQLPEGFRPD FRPKIPYSES IKEMLTTFGT ATYKVGLKVH 1330 1340 1350 1360 1370 1380 PNEEDPRVPI MCWGSCAYTI QSIERILSDE DKPLFGPLPC RLDDCLRSLT RFAAAHWTVA 1390 1400 1410 1420 1430 1440 SVSVVQGHFC KLFASLVPND SHEELPCILD IDMFHLLVGL VLAFPALQCQ DFSGISLGTG 1450 1460 1470 1480 1490 1500 DLHIFHLVTM AHIIQILLTS CTEENGMDQE NPPCEEESAV LALYKTLHQY TGSALKEIPS 1510 1520 1530 1540 1550 1560 GWHLWRSVRA GIMPFLKCSA LFFHYLNGVP SPPDIQVPGT SHFEHLCSYL SLPNNLICLF 1570 1580 1590 1600 1610 1620 QENSEIMNSL IESWCRNSEV KRYLEGERDA IRYPRESNKL INLPEDYSSL INQASNFSCP 1630 1640 1650 1660 1670 1680 KSGGDKSRAP TLCLVCGSLL CSQSYCCQTE LEGEDVGACT AHTYSCGSGV GIFLRVRECQ 1690 1700 1710 1720 1730 1740 VLFLAGKTKG CFYSPPYLDD YGETDQGLRR GNPLHLCKER FKKIQKLWHQ HSVTEEIGHA 1750 QEANQTLVGI DWQHL
	Isoform 2 [UniParc]. Checksum: D1A9B29DF254AE1F	FASTA	439	49,978
	10 20 30 40 50 60 MASELEPEVQ AIDRSLLECS AEEIAGKWLQ ATDLTREVYQ HLAHYVPKIY CRGPNPFPQK 70 80 90 100 110 120 EDMLAQHVLL GPMEWYLCGE DPAFGFPKLE QANKPSHLCG RVFKVGEPTY SCRDCAVDPT 130 140 150 160 170 180 CVLCMECFLG SIHRDHRYRM TTSGGGGFCD CGDTEAWKEG PYCQKHELNT SEIEEEEDPL 190 200 210 220 230 240 VHLSEDVIAR TYNIFAITFR YAVEILTWEK ESELPADLEM VEKSDTYYCM LFNDEVHTYE 250 260 270 280 290 300 QVIYTLQKAV NCTQKEAIGF ATTVDRDGRR SVRYGDFQYC EQAKSVIVRN TSRQTKPLKV 310 320 330 340 350 360 QVMHSSIVAH QNFGLKLLSW LGSIIGYSDG LRRILCQVGL QEGPDGENSS LVDRLMLSDS 370 380 390 400 410 420 KLWKGARSVY HQLFMSSLLM DLKYKKLFAV RFAKNYERLQ SDYVTDDHDR EFSVADLSVQ 430 IFTVPSLFSI SAGRSGSPL
	Isoform 3 [UniParc]. Checksum: EC2722AF55B26BD3	FASTA	575	66,052
	10 20 30 40 50 60 MYAGNIPIYK TESRSRNEQG MDPITRQVGQ HIEMEPEWEA AFTLQMKLTH VISMMQDWCA 70 80 90 100 110 120 SDEKVLIEAY KKCLAVLMQC HGGYTDGEQP ITLSICGHSV ETIRYCVSQE KVSIHLPVSR 130 140 150 160 170 180 LLAGLHVLLS KSEVAYKFPE LLPLSELSPP MLIEHPLRCL VLCAQVHAGM WRRNGFSLVN 190 200 210 220 230 240 QIYYYHNVKC RREMFDKDVV MLQTGVSMMD PNHFLMIMLS RFELYQIFST PDYGKRFSSE 250 260 270 280 290 300 ITHKDVVQQN NTLIEEMLYL IIMLVGERFS PGVGQVNATD EIKREIIHQL SIKPMAHSEL 310 320 330 340 350 360 VKSLPEDENK ETGMESVIEA VAHFKKPGLT GRGMYELKPE CAKEFNLYFY HFSRAEQSKA 370 380 390 400 410 420 EEAQRKLKRQ NREDTALPPP VLPPFCPLFA SLVNILQSDV MLCIMGTILQ WAVEHNGYAW 430 440 450 460 470 480 SESMLQRVLH LIGMALQEEK QHLENVTEEH VVTFTFTQKI SKPGEAPKNS PSILAMLETL 490 500 510 520 530 540 QNAPYLEVHK DMIRWILKTF NAVKKMRESS PTSPVAETEG TIMEEHNFRV QGTKTKLRGR 550 560 570 EKQRLPDCAE KRSWLRCLKC SGILLMKTKN SFSRH
	Isoform 4 [UniParc]. Checksum: 6FE8C7C600C162EE	FASTA	1,755	200,540
	10 20 30 40 50 60 MASELEPEVQ AIDRSLLECS AEEIAGKWLQ ATDLTREVYQ HLAHYVPKIY CRGPNPFPQK 70 80 90 100 110 120 EDMLAQHVLL GPMEWYLCGE DPAFGFPKLE QANKPSHLCG RVFKVGEPTY SCRDCAVDPT 130 140 150 160 170 180 CVLCMECFLG SIHRDHRYRM TTSGGGGFCD CGDTEAWKEG PYCQKHELNT SEIEEEEDPL 190 200 210 220 230 240 VHLSEDVIAR TYNIFAITFR YAVEILTWEK ESELPADLEM VEKSDTYYCM LFNDEVHTYE 250 260 270 280 290 300 QVIYTLQKAV NCTQKEAIGF ATTVDRDGRR SVRYGDFQYC EQAKSVIVRN TSRQTKPLKV 310 320 330 340 350 360 QVMHSSIVAH QNFGLKLLSW LGSIIGYSDG LRRILCQVGL QEGPDGENSS LVDRLMLSDS 370 380 390 400 410 420 KLWKGARSVY HQLFMSSLLM DLKYKKLFAV RFAKNYERLQ SDYVTDDHDR EFSVADLSVQ 430 440 450 460 470 480 IFTVPSLARM LITEENLMSI IIKTFMDHLR HRDAQGRFQF ERYTALQAFK FRRVQSLILD 490 500 510 520 530 540 LKYVLISKPT EWSDELRQKF LEGFDAFLEL LKCMQGMDPI TRQVGQHIEM EPEWEAAFTL 550 560 570 580 590 600 QMKLTHVISM MQDWCASDEK VLIEAYKKCL AVLMQCHGGY TDGEQPITLS ICGHSVETIR 610 620 630 640 650 660 YCVSQEKVSI HLPVSRLLAG LHVLLSKSEV AYKFPELLPL SELSPPMLIE HPLRCLVLCA 670 680 690 700 710 720 QVHAGMWRRN GFSLVNQIYY YHNVKCRREM FDKDVVMLQT GVSMMDPNHF LMIMLSRFEL 730 740 750 760 770 780 YQIFSTPDYG KRFSSEITHK DVVQQNNTLI EEMLYLIIML VGERFSPGVG QVNATDEIKR 790 800 810 820 830 840 EIIHQLSIKP MAHSELVKSL PEDENKETGM ESVIEAVAHF KKPGLTGRGM YELKPECAKE 850 860 870 880 890 900 FNLYFYHFSR AEQSKAEEAQ RKLKRQNRED TALPPPVLPP FCPLFASLVN ILQSDVMLCI 910 920 930 940 950 960 MGTILQWAVE HNGYAWSESM LQRVLHLIGM ALQEEKQHLE NVTEEHVVTF TFTQKISKPG 970 980 990 1000 1010 1020 EAPKNSPSIL AMLETLQNAP YLEVHKDMIR WILKTFNAVK KMRESSPTSP VAETEGTIME 1030 1040 1050 1060 1070 1080 ESSRDKDKAE RKRKAEIARL RREKIMAQMS EMQRHFIDEN KELFQQTLEL DASTSAVLDH 1090 1100 1110 1120 1130 1140 SPVASDMTLT ALGPAQTQVP EQRQFVTCIL CQEEQEVKVE SRAMVLAAFV QRSTVLSKNR 1150 1160 1170 1180 1190 1200 SKFIQDPEKY DPLFMHPDLS CGTHTSSCGH IMHAHCWQRY FDSVQAKEQR RQQRLRLHTS 1210 1220 1230 1240 1250 1260 YDVENGEFLC PLCECLSNTV IPLLLPPRNI FNNRLNFSDQ PNLTQWIRTI SQQIKALQFL 1270 1280 1290 1300 1310 1320 RKEESTPNNA STKNSENVDE LQLPEGFRPD FRPKIPYSES IKEMLTTFGT ATYKVGLKVH 1330 1340 1350 1360 1370 1380 PNEEDPRVPI MCWGSCAYTI QSIERILSDE DKPLFGPLPC RLDDCLRSLT RFAAAHWTVA 1390 1400 1410 1420 1430 1440 SVSVVQGHFC KLFASLVPND SHEELPCILD IDMFHLLVGL VLAFPALQCQ DFSGISLGTG 1450 1460 1470 1480 1490 1500 DLHIFHLVTM AHIIQILLTS CTEENGMDQE NPPCEEESAV LALYKTLHQY TGSALKEIPS 1510 1520 1530 1540 1550 1560 GWHLWRSVRA GIMPFLKCSA LFFHYLNGVP SPPDIQVPGT SHFEHLCSYL SLPNNLICLF 1570 1580 1590 1600 1610 1620 QENSEIMNSL IESWCRNSEV KRYLEGERDA IRYPRESNKL INLPEDYSSL INQASNFSCP 1630 1640 1650 1660 1670 1680 KSGGDKSRAP TLCLVCGSLL CSQSYCCQTE LEGEDVGACT AHTYSCGSGV GIFLRVRECQ 1690 1700 1710 1720 1730 1740 VLFLAGKTKG CFYSPPYLDD YGETDQGLRR GNPLHLCKER FKKIQKLWHQ HSVTEEIGHA 1750 QEANQTLVGI DWQHL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Regulation of protein catabolism by muscle-specific and cytokine-inducible ubiquitin ligase E3alpha-II during cancer cachexia." Kwak K.S., Zhou X., Solomon V., Baracos V.E., Davis J., Bannon A.W., Boyle W.J., Lacey D.L., Han H.Q. Cancer Res. 64:8193-8198(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, FUNCTION.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Testis.
[3]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Ovary.
[5]	"Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Nagase T., Ishikawa K., Nakajima D., Ohira M., Seki N., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 4:141-150(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 481-1755 (ISOFORMS 1/4), VARIANT THR-1095. Tissue: Brain.
[6]	"RECQL4, mutated in the Rothmund-Thomson and RAPADILINO syndromes, interacts with ubiquitin ligases UBR1 and UBR2 of the N-end rule pathway." Yin J., Kwon Y.T., Varshavsky A., Wang W. Hum. Mol. Genet. 13:2421-2430(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RECQL4, IDENTIFICATION BY MASS SPECTROMETRY.
[7]	"Deficiency of UBR1, a ubiquitin ligase of the N-end rule pathway, causes pancreatic dysfunction, malformations and mental retardation (Johanson-Blizzard syndrome)." Zenker M., Mayerle J., Lerch M.M., Tagariello A., Zerres K., Durie P.R., Beier M., Hulskamp G., Guzman C., Rehder H., Beemer F.A., Hamel B.C.J., Vanlieferinghen P., Gershoni-Baruch R., Vieira M.W., Dumic M., Auslender R., Gil-da-Silva-Lopes V.L. , Steinlicht S., Rauh M., Shalev S.A., Thiel C., Winterpacht A., Kwon Y.T., Varshavsky A., Reis A. Nat. Genet. 37:1345-1350(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
[10]	"Role of N-end rule ubiquitin ligases UBR1 and UBR2 in regulating the leucine-mTOR signaling pathway." Kume K., Iizumi Y., Shimada M., Ito Y., Kishi T., Yamaguchi Y., Handa H. Genes Cells 15:339-349(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY061884 mRNA. Translation: AAL32101.1.
AK125795 mRNA. Translation: BAC86295.1.
AL049843, AL136223, AL391814 Genomic DNA. Translation: CAI20399.1.
AL049843, AL136223, AL391814 Genomic DNA. Translation: CAI95685.1.
AL136223, AL391814 Genomic DNA. Translation: CAI19919.1.
AL136223, AL049843, AL391814 Genomic DNA. Translation: CAI19920.1.
AL136223, AL049843, AL391814 Genomic DNA. Translation: CAI95666.1.
AL391814, AL136223 Genomic DNA. Translation: CAI14737.1.
AL391814, AL136223, AL049843 Genomic DNA. Translation: CAI14738.1.
AL391814, AL049843, AL136223 Genomic DNA. Translation: CAI95298.1.
BC024217 mRNA. Translation: AAH24217.1.
BC064512 mRNA. Translation: AAH64512.1.
AB002347 mRNA. Translation: BAA20806.1.

IPI

IPI00217407.
IPI00445539.
IPI00640085.
IPI00640329.

RefSeq

NP_001171730.1. NM_001184801.1.
NP_056070.1. NM_015255.2.

UniGene

Hs.529925.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3NY2	X-ray	2.61	A/B/C/D/E/F/G/H	98-167	[»]
3NY3	X-ray	1.60	A	98-167	[»]

ProteinModelPortal

Q8IWV8.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-38165N.

IntAct

Q8IWV8. 7 interactions.

STRING

9606.ENSP00000361990.

PTM databases

PhosphoSite

Q8IWV8.

Polymorphism databases

DMDM

73622073.

Proteomic databases

PaxDb

Q8IWV8.

PRIDE

Q8IWV8.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000372883; ENSP00000361974; ENSG00000024048.
ENST00000372899; ENSP00000361990; ENSG00000024048.
ENST00000372901; ENSP00000361992; ENSG00000024048.
ENST00000372903; ENSP00000361994; ENSG00000024048.

GeneID

23304.

KEGG

hsa:23304.

UCSC

uc003osf.3. human.
uc011dur.2. human.
uc011dus.2. human.

Organism-specific databases

CTD

23304.

GeneCards

GC06P042531.

HGNC

HGNC:21289. UBR2.

HPA

HPA027869.
HPA027880.

MIM

609134. gene.

neXtProt

NX_Q8IWV8.

PharmGKB

PA128394621.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG310244.

HOGENOM

HOG000231769.

HOVERGEN

HBG080426.

KO

K10626.

OMA

PVLPPFC.

OrthoDB

EOG42NHZG.

PhylomeDB

Q8IWV8.

Enzyme and pathway databases

Reactome

REACT_6900. Immune System.

UniPathway

UPA00143.

Gene expression databases

ArrayExpress

Q8IWV8.

Bgee

Q8IWV8.

CleanEx

HS_UBR2.

Genevestigator

Q8IWV8.

GermOnline

ENSG00000024048. Homo sapiens.

Family and domain databases

Gene3D

3.30.1390.10. 1 hit.

InterPro

IPR003769. ClpS_core.
IPR014719. Ribosomal_L7/12_C/ClpS-like.
IPR003126. Znf_N-recognin.
IPR013993. Znf_N-recognin_met.
IPR001841. Znf_RING.
[Graphical view]

Pfam

PF02617. ClpS. 1 hit.
PF02207. zf-UBR. 1 hit.
[Graphical view]

SMART

SM00184. RING. 1 hit.
SM00396. ZnF_UBR1. 1 hit.
[Graphical view]

SUPFAM

SSF54736. Ribosomal_L7/12_C/ClpS-like. 1 hit.

PROSITE

PS00518. ZF_RING_1. False negative.
PS50089. ZF_RING_2. False negative.
PS51157. ZF_UBR. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q8IWV8.

GenomeRNAi

23304.

NextBio

45149.

SOURCE

Search...

Entry information

Entry name

UBR2_HUMAN

Accession

Primary (citable) accession number: Q8IWV8
Secondary accession number(s): O15057 , Q4VXK2, Q5TFH6, Q6P2I2, Q6ZUD0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.