Q8IWV1 (LAX1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lymphocyte transmembrane adapter 1

Alternative name(s):
Linker for activation of X cells
Membrane-associated adapter protein LAX

Gene names

Name:	LAX1
Synonyms:	LAX

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and BCR (B-cell antigen receptor)-mediated signaling in B-cells. Ref.1
Subunit structure	When phosphorylated, interacts with GRB2, PIK3R1 and GRAP2. Ref.1
Subcellular location	Cell membrane; Single-pass type III membrane protein Ref.1.
Tissue specificity	Expressed in spleen, thymus, and peripheral blood leukocytes. Expressed in several B-, T-, NK and monocyte cell lines. Ref.1
Induction	Up-regulated in T-cells following TCR engagement. Ref.4
Post-translational modification	Phosphorylated on tyrosines by Syk, Lck or ZAP70 upon TCR or BCR activation; which leads to the recruitment of GRB2, PIK3R1 and GRAP2. Ref.1 Ref.5
Sequence caution	The sequence AAH69650.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Adaptive immunity Immunity
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	B cell activation Inferred from direct assay Ref.1. Source: HGNC immune response Inferred from direct assay Ref.1. Source: HGNC inactivation of MAPK activity Inferred from mutant phenotype Ref.1. Source: HGNC intracellular signal transduction Inferred from direct assay Ref.1. Source: HGNC negative regulation of T cell activation Inferred from direct assay Ref.1. Source: HGNC
Cellular component	Golgi apparatus Inferred from direct assay. Source: HPA integral to membrane Inferred from direct assay Ref.1. Source: HGNC plasma membrane Inferred from direct assay. Source: HPA
Molecular function	SH2 domain binding Inferred from direct assay Ref.1. Source: HGNC protein kinase binding Inferred from direct assay Ref.1. Source: HGNC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8IWV1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8IWV1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Lymphocyte transmembrane adapter 1		PRO_0000083329
Regions
	Topological domain	1 – 37	37	Extracellular Potential
	Transmembrane	38 – 58	21	Helical; Signal-anchor for type III membrane protein; Potential
	Topological domain	59 – 398	340	Cytoplasmic Potential
Amino acid modifications
	Modified residue	193	1	Phosphotyrosine Probable
	Modified residue	268	1	Phosphotyrosine Probable
	Modified residue	294	1	Phosphotyrosine Probable
	Modified residue	345	1	Phosphotyrosine Ref.5
	Modified residue	373	1	Phosphotyrosine Ref.5
Natural variations
	Alternative sequence	1 – 76	76	Missing in isoform 2.		VSP_016641
Experimental info
	Sequence conflict	152	1	V → A in BAA91101. Ref.2
	Sequence conflict	185	1	I → V in BAA91101. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 16FD4D8A56CEA980	FASTA	398	44,085
	10 20 30 40 50 60 MDGVTPTLST IRGRTLESST LHVTPRSLDR NKDQITNIFS GFAGLLAILL VVAVFCILWN 70 80 90 100 110 120 WNKRKKRQVP YLRVTVMPLL TLPQTRQRAK NIYDILPWRQ EDLGRHESRS MRIFSTESLL 130 140 150 160 170 180 SRNSESPEHV PSQAGNAFQE HTAHIHATEY AVGIYDNAMV PQMCGNLTPS AHCINVRASR 190 200 210 220 230 240 DCASISSEDS HDYVNVPTAE EIAETLASTK SPSRNLFVLP STQKLEFTEE RDEGCGDAGD 250 260 270 280 290 300 CTSLYSPGAE DSDSLSNGEG SSQISNDYVN MTGLDLSAIQ ERQLWVAFQC CRDYENVPAA 310 320 330 340 350 360 DPSGSQQQAE KDVPSSNIGH VEDKTDDPGT HVQCVKRTFL ASGDYADFQP FTQSEDSQMK 370 380 390 HREEMSNEDS SDYENVLTAK LGGRDSEQGP GTQLLPDE
	Isoform 2 [UniParc]. Checksum: 39AE849D1B75EFC7	FASTA	322	35,476
	10 20 30 40 50 60 MPLLTLPQTR QRAKNIYDIL PWRQEDLGRH ESRSMRIFST ESLLSRNSES PEHVPSQAGN 70 80 90 100 110 120 AFQEHTAHIH ATEYAVGIYD NAMVPQMCGN LTPSAHCINV RASRDCASIS SEDSHDYVNV 130 140 150 160 170 180 PTAEEIAETL ASTKSPSRNL FVLPSTQKLE FTEERDEGCG DAGDCTSLYS PGAEDSDSLS 190 200 210 220 230 240 NGEGSSQISN DYVNMTGLDL SAIQERQLWV AFQCCRDYEN VPAADPSGSQ QQAEKDVPSS 250 260 270 280 290 300 NIGHVEDKTD DPGTHVQCVK RTFLASGDYA DFQPFTQSED SQMKHREEMS NEDSSDYENV 310 320 LTAKLGGRDS EQGPGTQLLP DE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a novel gene encoding a membrane-associated adaptor protein (LAX) in lymphocyte signaling." Zhu M., Janssen E., Leung K., Zhang W. J. Biol. Chem. 277:46151-46158(2002) [PubMed: 12359715] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH GRB2; PIK3R1 AND GRAP2, PHOSPHORYLATION, FUNCTION.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lymph.
[4]	"Negative regulation of lymphocyte activation by the adaptor protein LAX." Zhu M., Granillo O., Wen R., Yang K., Dai X., Wang D., Zhang W. J. Immunol. 174:5612-5619(2005) [PubMed: 15843560] [Abstract] Cited for: INDUCTION.
[5]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-345 AND TYR-373, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY090784 mRNA. Translation: AAM09818.1. AK000347 mRNA. Translation: BAA91101.1. BC069650 mRNA. Translation: AAH69650.1. Different initiation. BC089408 mRNA. Translation: AAH89408.1.
IPI	IPI00301971. IPI00642381.
RefSeq	NP_001129662.1. NM_001136190.1. NP_060243.2. NM_017773.3.
UniGene	Hs.272794.
3D structure databases
ProteinModelPortal	Q8IWV1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8IWV1.
PTM databases
PhosphoSite	Q8IWV1.
Polymorphism databases
DMDM	74728197.
Proteomic databases
PRIDE	Q8IWV1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000367217; ENSP00000356186; ENSG00000122188.
GeneID	54900.
KEGG	hsa:54900.
UCSC	uc001haa.1. human. uc001hab.1. human.
Organism-specific databases
CTD	54900.
GeneCards	GC01P203734.
H-InvDB	HIX0199807.
HGNC	HGNC:26005. LAX1.
HPA	CAB025358. HPA002461. HPA003887.
neXtProt	NX_Q8IWV1.
PharmGKB	PA142671569.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG07150.
GeneTree	ENSGT00390000014063.
HOGENOM	HBG127414.
HOVERGEN	HBG081892.
InParanoid	Q8IWV1.
OMA	TSNDYVN.
Gene expression databases
ArrayExpress	Q8IWV1.
Bgee	Q8IWV1.
CleanEx	HS_LAX1.
Genevestigator	Q8IWV1.
GermOnline	ENSG00000122188. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other
NextBio	57924.

Entry information

Entry name

LAX1_HUMAN

Accession

Primary (citable) accession number: Q8IWV1
Secondary accession number(s): Q6NSZ6, Q9NXB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.