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Protein

Lymphocyte transmembrane adapter 1

Gene

LAX1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and BCR (B-cell antigen receptor)-mediated signaling in B-cells.1 Publication

GO - Molecular functioni

  • protein kinase binding Source: HGNC
  • SH2 domain binding Source: HGNC

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • antigen receptor-mediated signaling pathway Source: Ensembl
  • B cell activation Source: HGNC
  • immune response Source: HGNC
  • immunoglobulin secretion Source: Ensembl
  • inactivation of MAPK activity Source: HGNC
  • intracellular signal transduction Source: HGNC
  • negative regulation of T cell activation Source: HGNC
Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Immunity

Enzyme and pathway databases

SignaLinkiQ8IWV1.

Names & Taxonomyi

Protein namesi
Recommended name:
Lymphocyte transmembrane adapter 1
Alternative name(s):
Linker for activation of X cells
Membrane-associated adapter protein LAX
Gene namesi
Name:LAX1
Synonyms:LAX
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:26005. LAX1.

Subcellular locationi

  • Cell membrane 1 Publication; Single-pass type III membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3737ExtracellularSequence analysisAdd
BLAST
Transmembranei38 – 5821Helical; Signal-anchor for type III membrane proteinSequence analysisAdd
BLAST
Topological domaini59 – 398340CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cytoplasm Source: HPA
  • Golgi apparatus Source: HPA
  • integral component of membrane Source: HGNC
  • membrane Source: UniProtKB
  • plasma membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671569.

Polymorphism and mutation databases

BioMutaiLAX1.
DMDMi74728197.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 398398Lymphocyte transmembrane adapter 1PRO_0000083329Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei193 – 1931Phosphotyrosine1 Publication
Modified residuei268 – 2681Phosphotyrosine1 Publication
Modified residuei294 – 2941Phosphotyrosine1 Publication
Modified residuei345 – 3451PhosphotyrosineCombined sources
Modified residuei373 – 3731PhosphotyrosineCombined sources

Post-translational modificationi

Phosphorylated on tyrosines by Syk, Lck or ZAP70 upon TCR or BCR activation; which leads to the recruitment of GRB2, PIK3R1 and GRAP2.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8IWV1.
PeptideAtlasiQ8IWV1.
PRIDEiQ8IWV1.

PTM databases

iPTMnetiQ8IWV1.
PhosphoSiteiQ8IWV1.

Expressioni

Tissue specificityi

Expressed in spleen, thymus, and peripheral blood leukocytes. Expressed in several B-, T-, NK and monocyte cell lines.1 Publication

Inductioni

Up-regulated in T-cells following TCR engagement.1 Publication

Gene expression databases

BgeeiENSG00000122188.
CleanExiHS_LAX1.
GenevisibleiQ8IWV1. HS.

Organism-specific databases

HPAiCAB025358.
HPA002461.
HPA003887.

Interactioni

Subunit structurei

When phosphorylated, interacts with GRB2, PIK3R1 and GRAP2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GRB7Q144513EBI-10232942,EBI-970191
STAMBPL1Q96FJ03EBI-10232942,EBI-745021

GO - Molecular functioni

  • protein kinase binding Source: HGNC
  • SH2 domain binding Source: HGNC

Protein-protein interaction databases

BioGridi120246. 6 interactions.
IntActiQ8IWV1. 5 interactions.
STRINGi9606.ENSP00000406970.

Structurei

3D structure databases

ProteinModelPortaliQ8IWV1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IVGH. Eukaryota.
ENOG41119TZ. LUCA.
GeneTreeiENSGT00390000014063.
HOGENOMiHOG000113286.
HOVERGENiHBG081892.
InParanoidiQ8IWV1.
OMAiTSNDYVN.
OrthoDBiEOG091G0FTE.
PhylomeDBiQ8IWV1.
TreeFamiTF337411.

Family and domain databases

InterProiIPR031393. LAX.
[Graphical view]
PfamiPF15681. LAX. 1 hit.
[Graphical view]
ProDomiPD319679. PD319679. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8IWV1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGVTPTLST IRGRTLESST LHVTPRSLDR NKDQITNIFS GFAGLLAILL
60 70 80 90 100
VVAVFCILWN WNKRKKRQVP YLRVTVMPLL TLPQTRQRAK NIYDILPWRQ
110 120 130 140 150
EDLGRHESRS MRIFSTESLL SRNSESPEHV PSQAGNAFQE HTAHIHATEY
160 170 180 190 200
AVGIYDNAMV PQMCGNLTPS AHCINVRASR DCASISSEDS HDYVNVPTAE
210 220 230 240 250
EIAETLASTK SPSRNLFVLP STQKLEFTEE RDEGCGDAGD CTSLYSPGAE
260 270 280 290 300
DSDSLSNGEG SSQISNDYVN MTGLDLSAIQ ERQLWVAFQC CRDYENVPAA
310 320 330 340 350
DPSGSQQQAE KDVPSSNIGH VEDKTDDPGT HVQCVKRTFL ASGDYADFQP
360 370 380 390
FTQSEDSQMK HREEMSNEDS SDYENVLTAK LGGRDSEQGP GTQLLPDE
Length:398
Mass (Da):44,085
Last modified:March 1, 2003 - v1
Checksum:i16FD4D8A56CEA980
GO
Isoform 2 (identifier: Q8IWV1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: Missing.

Show »
Length:322
Mass (Da):35,476
Checksum:i39AE849D1B75EFC7
GO
Isoform 3 (identifier: Q8IWV1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: MDGVTPTLSTIRGRTLESSTLHVTPRSLD → MRSHFLQWALATS

Show »
Length:382
Mass (Da):42,491
Checksum:iC2DFAE47A5807E66
GO

Sequence cautioni

The sequence AAH69650 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti152 – 1521V → A in BAA91101 (PubMed:14702039).Curated
Sequence conflicti185 – 1851I → V in BAA91101 (PubMed:14702039).Curated
Sequence conflicti320 – 3201H → R in BAH13480 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7676Missing in isoform 2. 1 PublicationVSP_016641Add
BLAST
Alternative sequencei1 – 2929MDGVT…PRSLD → MRSHFLQWALATS in isoform 3. 1 PublicationVSP_046972Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY090784 mRNA. Translation: AAM09818.1.
AK000347 mRNA. Translation: BAA91101.1.
AK301421 mRNA. Translation: BAH13480.1.
AC114402 Genomic DNA. No translation available.
CH471067 Genomic DNA. Translation: EAW91491.1.
BC069650 mRNA. Translation: AAH69650.1. Different initiation.
BC089408 mRNA. Translation: AAH89408.1.
CCDSiCCDS1441.2. [Q8IWV1-1]
CCDS44297.1. [Q8IWV1-3]
RefSeqiNP_001129662.1. NM_001136190.1. [Q8IWV1-3]
NP_001269807.1. NM_001282878.1. [Q8IWV1-2]
NP_060243.2. NM_017773.3. [Q8IWV1-1]
XP_006711460.1. XM_006711397.3. [Q8IWV1-1]
UniGeneiHs.272794.

Genome annotation databases

EnsembliENST00000367217; ENSP00000356186; ENSG00000122188. [Q8IWV1-3]
ENST00000442561; ENSP00000406970; ENSG00000122188. [Q8IWV1-1]
GeneIDi54900.
KEGGihsa:54900.
UCSCiuc001haa.4. human. [Q8IWV1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY090784 mRNA. Translation: AAM09818.1.
AK000347 mRNA. Translation: BAA91101.1.
AK301421 mRNA. Translation: BAH13480.1.
AC114402 Genomic DNA. No translation available.
CH471067 Genomic DNA. Translation: EAW91491.1.
BC069650 mRNA. Translation: AAH69650.1. Different initiation.
BC089408 mRNA. Translation: AAH89408.1.
CCDSiCCDS1441.2. [Q8IWV1-1]
CCDS44297.1. [Q8IWV1-3]
RefSeqiNP_001129662.1. NM_001136190.1. [Q8IWV1-3]
NP_001269807.1. NM_001282878.1. [Q8IWV1-2]
NP_060243.2. NM_017773.3. [Q8IWV1-1]
XP_006711460.1. XM_006711397.3. [Q8IWV1-1]
UniGeneiHs.272794.

3D structure databases

ProteinModelPortaliQ8IWV1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120246. 6 interactions.
IntActiQ8IWV1. 5 interactions.
STRINGi9606.ENSP00000406970.

PTM databases

iPTMnetiQ8IWV1.
PhosphoSiteiQ8IWV1.

Polymorphism and mutation databases

BioMutaiLAX1.
DMDMi74728197.

Proteomic databases

PaxDbiQ8IWV1.
PeptideAtlasiQ8IWV1.
PRIDEiQ8IWV1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000367217; ENSP00000356186; ENSG00000122188. [Q8IWV1-3]
ENST00000442561; ENSP00000406970; ENSG00000122188. [Q8IWV1-1]
GeneIDi54900.
KEGGihsa:54900.
UCSCiuc001haa.4. human. [Q8IWV1-1]

Organism-specific databases

CTDi54900.
GeneCardsiLAX1.
HGNCiHGNC:26005. LAX1.
HPAiCAB025358.
HPA002461.
HPA003887.
neXtProtiNX_Q8IWV1.
PharmGKBiPA142671569.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IVGH. Eukaryota.
ENOG41119TZ. LUCA.
GeneTreeiENSGT00390000014063.
HOGENOMiHOG000113286.
HOVERGENiHBG081892.
InParanoidiQ8IWV1.
OMAiTSNDYVN.
OrthoDBiEOG091G0FTE.
PhylomeDBiQ8IWV1.
TreeFamiTF337411.

Enzyme and pathway databases

SignaLinkiQ8IWV1.

Miscellaneous databases

GenomeRNAii54900.
PROiQ8IWV1.

Gene expression databases

BgeeiENSG00000122188.
CleanExiHS_LAX1.
GenevisibleiQ8IWV1. HS.

Family and domain databases

InterProiIPR031393. LAX.
[Graphical view]
PfamiPF15681. LAX. 1 hit.
[Graphical view]
ProDomiPD319679. PD319679. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Entry informationi

Entry nameiLAX1_HUMAN
AccessioniPrimary (citable) accession number: Q8IWV1
Secondary accession number(s): B7Z744
, J3KP69, Q6NSZ6, Q9NXB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.