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Protein

Extracellular sulfatase Sulf-1

Gene

SULF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Diminishes HSPG (heparan sulfate proteoglycans) sulfation, inhibits signaling by heparin-dependent growth factors, diminishes proliferation, and facilitates apoptosis in response to exogenous stimulation.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

pH dependencei

Optimum pH is 7.0-8.0.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi51CalciumBy similarity1
Metal bindingi52CalciumBy similarity1
Metal bindingi87Calcium; via 3-oxoalanineBy similarity1
Metal bindingi316CalciumBy similarity1
Metal bindingi317CalciumBy similarity1

GO - Molecular functioni

  • arylsulfatase activity Source: UniProtKB
  • calcium ion binding Source: InterPro
  • N-acetylglucosamine-6-sulfatase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Extracellular sulfatase Sulf-1 (EC:3.1.6.-)
Short name:
hSulf-1
Gene namesi
Name:SULF1
Synonyms:KIAA1077
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:20391. SULF1.

Subcellular locationi

GO - Cellular componenti

  • cell surface Source: UniProtKB
  • endoplasmic reticulum Source: UniProtKB
  • extracellular space Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • Golgi stack Source: UniProtKB-SubCell
  • membrane raft Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi87 – 88CC → AA: Loss of arylsulfatase activity and loss of ability to modulate apoptosis. 2 Publications2

Organism-specific databases

DisGeNETi23213.
OpenTargetsiENSG00000137573.
PharmGKBiPA134861022.

Polymorphism and mutation databases

BioMutaiSULF1.
DMDMi33112447.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000003343423 – 871Extracellular sulfatase Sulf-1Add BLAST849

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi64N-linked (GlcNAc...)Sequence analysis1
Modified residuei873-oxoalanine (Cys)By similarity1
Glycosylationi111N-linked (GlcNAc...)Sequence analysis1
Glycosylationi131N-linked (GlcNAc...)Sequence analysis1
Glycosylationi148N-linked (GlcNAc...)Sequence analysis1
Glycosylationi170N-linked (GlcNAc...)Sequence analysis1
Glycosylationi197N-linked (GlcNAc...)1 Publication1
Glycosylationi240N-linked (GlcNAc...)Sequence analysis1
Glycosylationi623N-linked (GlcNAc...)Sequence analysis1
Glycosylationi773N-linked (GlcNAc...)Sequence analysis1
Glycosylationi783N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8IWU6.
PeptideAtlasiQ8IWU6.
PRIDEiQ8IWU6.

PTM databases

iPTMnetiQ8IWU6.
PhosphoSitePlusiQ8IWU6.

Expressioni

Tissue specificityi

Expressed at highest levels in testis, stomach, skeletal muscle, lung, kidney, pancreas, small intestine and colon. It is also detected in normal ovarian surface epithelial cells. Down-regulation seen in ovarian carcinoma cell lines, ovarian cancers, breast, pancreatic, renal and hepatocellular carcinoma cell lines.

Gene expression databases

BgeeiENSG00000137573.
CleanExiHS_SULF1.
ExpressionAtlasiQ8IWU6. baseline and differential.
GenevisibleiQ8IWU6. HS.

Organism-specific databases

HPAiHPA054728.

Interactioni

Protein-protein interaction databases

BioGridi116820. 6 interactors.
STRINGi9606.ENSP00000260128.

Structurei

3D structure databases

ProteinModelPortaliQ8IWU6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3731. Eukaryota.
COG3119. LUCA.
GeneTreeiENSGT00400000022041.
HOGENOMiHOG000290161.
HOVERGENiHBG056431.
InParanoidiQ8IWU6.
KOiK14607.
OMAiRHDEGHR.
OrthoDBiEOG091G01S2.
PhylomeDBiQ8IWU6.
TreeFamiTF313545.

Family and domain databases

Gene3Di3.40.720.10. 3 hits.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR014615. Extracellular_sulfatase.
IPR024609. Extracellular_sulfatase_C.
IPR024607. Sulfatase_CS.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF12548. DUF3740. 1 hit.
PF00884. Sulfatase. 1 hit.
[Graphical view]
PIRSFiPIRSF036665. Sulf1. 1 hit.
SUPFAMiSSF53649. SSF53649. 3 hits.
PROSITEiPS00523. SULFATASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8IWU6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYSCCALVL AVLGTELLGS LCSTVRSPRF RGRIQQERKN IRPNIILVLT
60 70 80 90 100
DDQDVELGSL QVMNKTRKIM EHGGATFINA FVTTPMCCPS RSSMLTGKYV
110 120 130 140 150
HNHNVYTNNE NCSSPSWQAM HEPRTFAVYL NNTGYRTAFF GKYLNEYNGS
160 170 180 190 200
YIPPGWREWL GLIKNSRFYN YTVCRNGIKE KHGFDYAKDY FTDLITNESI
210 220 230 240 250
NYFKMSKRMY PHRPVMMVIS HAAPHGPEDS APQFSKLYPN ASQHITPSYN
260 270 280 290 300
YAPNMDKHWI MQYTGPMLPI HMEFTNILQR KRLQTLMSVD DSVERLYNML
310 320 330 340 350
VETGELENTY IIYTADHGYH IGQFGLVKGK SMPYDFDIRV PFFIRGPSVE
360 370 380 390 400
PGSIVPQIVL NIDLAPTILD IAGLDTPPDV DGKSVLKLLD PEKPGNRFRT
410 420 430 440 450
NKKAKIWRDT FLVERGKFLR KKEESSKNIQ QSNHLPKYER VKELCQQARY
460 470 480 490 500
QTACEQPGQK WQCIEDTSGK LRIHKCKGPS DLLTVRQSTR NLYARGFHDK
510 520 530 540 550
DKECSCRESG YRASRSQRKS QRQFLRNQGT PKYKPRFVHT RQTRSLSVEF
560 570 580 590 600
EGEIYDINLE EEEELQVLQP RNIAKRHDEG HKGPRDLQAS SGGNRGRMLA
610 620 630 640 650
DSSNAVGPPT TVRVTHKCFI LPNDSIHCER ELYQSARAWK DHKAYIDKEI
660 670 680 690 700
EALQDKIKNL REVRGHLKRR KPEECSCSKQ SYYNKEKGVK KQEKLKSHLH
710 720 730 740 750
PFKEAAQEVD SKLQLFKENN RRRKKERKEK RRQRKGEECS LPGLTCFTHD
760 770 780 790 800
NNHWQTAPFW NLGSFCACTS SNNNTYWCLR TVNETHNFLF CEFATGFLEY
810 820 830 840 850
FDMNTDPYQL TNTVHTVERG ILNQLHVQLM ELRSCQGYKQ CNPRPKNLDV
860 870
GNKDGGSYDL HRGQLWDGWE G
Length:871
Mass (Da):101,027
Last modified:March 1, 2003 - v1
Checksum:i9A90ADB280304364
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti49L → P in AAO33315 (PubMed:12686563).Curated1
Sequence conflicti728K → R in BAC11258 (PubMed:14702039).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY101175 mRNA. Translation: AAM76860.1.
AF545571 mRNA. Translation: AAO33315.1.
AB029000 mRNA. Translation: BAA83029.1.
AK074873 mRNA. Translation: BAC11258.1.
CCDSiCCDS6204.1.
RefSeqiNP_001121676.1. NM_001128204.1.
NP_001121677.1. NM_001128205.1.
NP_001121678.1. NM_001128206.1.
NP_055985.2. NM_015170.2.
UniGeneiHs.409602.

Genome annotation databases

EnsembliENST00000260128; ENSP00000260128; ENSG00000137573.
ENST00000402687; ENSP00000385704; ENSG00000137573.
ENST00000419716; ENSP00000390315; ENSG00000137573.
ENST00000458141; ENSP00000403040; ENSG00000137573.
GeneIDi23213.
KEGGihsa:23213.
UCSCiuc003xyd.3. human.

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY101175 mRNA. Translation: AAM76860.1.
AF545571 mRNA. Translation: AAO33315.1.
AB029000 mRNA. Translation: BAA83029.1.
AK074873 mRNA. Translation: BAC11258.1.
CCDSiCCDS6204.1.
RefSeqiNP_001121676.1. NM_001128204.1.
NP_001121677.1. NM_001128205.1.
NP_001121678.1. NM_001128206.1.
NP_055985.2. NM_015170.2.
UniGeneiHs.409602.

3D structure databases

ProteinModelPortaliQ8IWU6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116820. 6 interactors.
STRINGi9606.ENSP00000260128.

PTM databases

iPTMnetiQ8IWU6.
PhosphoSitePlusiQ8IWU6.

Polymorphism and mutation databases

BioMutaiSULF1.
DMDMi33112447.

Proteomic databases

PaxDbiQ8IWU6.
PeptideAtlasiQ8IWU6.
PRIDEiQ8IWU6.

Protocols and materials databases

DNASUi23213.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000260128; ENSP00000260128; ENSG00000137573.
ENST00000402687; ENSP00000385704; ENSG00000137573.
ENST00000419716; ENSP00000390315; ENSG00000137573.
ENST00000458141; ENSP00000403040; ENSG00000137573.
GeneIDi23213.
KEGGihsa:23213.
UCSCiuc003xyd.3. human.

Organism-specific databases

CTDi23213.
DisGeNETi23213.
GeneCardsiSULF1.
HGNCiHGNC:20391. SULF1.
HPAiHPA054728.
MIMi610012. gene.
neXtProtiNX_Q8IWU6.
OpenTargetsiENSG00000137573.
PharmGKBiPA134861022.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3731. Eukaryota.
COG3119. LUCA.
GeneTreeiENSGT00400000022041.
HOGENOMiHOG000290161.
HOVERGENiHBG056431.
InParanoidiQ8IWU6.
KOiK14607.
OMAiRHDEGHR.
OrthoDBiEOG091G01S2.
PhylomeDBiQ8IWU6.
TreeFamiTF313545.

Miscellaneous databases

ChiTaRSiSULF1. human.
GeneWikiiSULF1.
GenomeRNAii23213.
PROiQ8IWU6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000137573.
CleanExiHS_SULF1.
ExpressionAtlasiQ8IWU6. baseline and differential.
GenevisibleiQ8IWU6. HS.

Family and domain databases

Gene3Di3.40.720.10. 3 hits.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR014615. Extracellular_sulfatase.
IPR024609. Extracellular_sulfatase_C.
IPR024607. Sulfatase_CS.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF12548. DUF3740. 1 hit.
PF00884. Sulfatase. 1 hit.
[Graphical view]
PIRSFiPIRSF036665. Sulf1. 1 hit.
SUPFAMiSSF53649. SSF53649. 3 hits.
PROSITEiPS00523. SULFATASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSULF1_HUMAN
AccessioniPrimary (citable) accession number: Q8IWU6
Secondary accession number(s): Q86YV8, Q8NCA2, Q9UPS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.