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Protein

Extracellular sulfatase Sulf-2

Gene

SULF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

pH dependencei

Optimum pH is 7.0-8.0.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi52CalciumBy similarity1
Metal bindingi53CalciumBy similarity1
Metal bindingi88Calcium; via 3-oxoalanineBy similarity1
Metal bindingi317CalciumBy similarity1
Metal bindingi318CalciumBy similarity1

GO - Molecular functioni

  • arylsulfatase activity Source: UniProtKB
  • calcium ion binding Source: InterPro
  • N-acetylglucosamine-6-sulfatase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Extracellular sulfatase Sulf-2 (EC:3.1.6.-)
Short name:
hSulf-2
Gene namesi
Name:SULF2
Synonyms:KIAA1247
ORF Names:UNQ559/PRO1120
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:20392. SULF2.

Subcellular locationi

GO - Cellular componenti

  • cell surface Source: UniProtKB
  • endoplasmic reticulum Source: UniProtKB
  • extracellular space Source: UniProtKB
  • Golgi stack Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi88 – 89CC → AA: Loss of arylsulfatase activity. 1 Publication2

Organism-specific databases

DisGeNETi55959.
OpenTargetsiENSG00000196562.
PharmGKBiPA134902131.

Polymorphism and mutation databases

BioMutaiSULF2.
DMDMi33112446.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000003344025 – 870Extracellular sulfatase Sulf-2Add BLAST846

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi65N-linked (GlcNAc...)Sequence analysis1
Modified residuei883-oxoalanine (Cys)By similarity1
Glycosylationi112N-linked (GlcNAc...)Sequence analysis1
Glycosylationi132N-linked (GlcNAc...)Sequence analysis1
Glycosylationi149N-linked (GlcNAc...)Sequence analysis1
Glycosylationi171N-linked (GlcNAc...)Sequence analysis1
Glycosylationi198N-linked (GlcNAc...)1 Publication1
Glycosylationi241N-linked (GlcNAc...)Sequence analysis1
Glycosylationi561N-linked (GlcNAc...)Sequence analysis1
Glycosylationi608N-linked (GlcNAc...)Sequence analysis1
Glycosylationi717N-linked (GlcNAc...)Sequence analysis1
Glycosylationi754N-linked (GlcNAc...)Sequence analysis1
Glycosylationi764N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8IWU5.
PaxDbiQ8IWU5.
PeptideAtlasiQ8IWU5.
PRIDEiQ8IWU5.
TopDownProteomicsiQ8IWU5-2. [Q8IWU5-2]

PTM databases

iPTMnetiQ8IWU5.
PhosphoSitePlusiQ8IWU5.

Expressioni

Tissue specificityi

Expressed at highest levels in the ovary, skeletal muscle, stomach, brain, uterus, heart, kidney and placenta.

Gene expression databases

BgeeiENSG00000196562.
CleanExiHS_SULF2.
ExpressionAtlasiQ8IWU5. baseline and differential.
GenevisibleiQ8IWU5. HS.

Organism-specific databases

HPAiHPA002325.

Interactioni

Protein-protein interaction databases

BioGridi121010. 17 interactors.
IntActiQ8IWU5. 2 interactors.
STRINGi9606.ENSP00000353007.

Structurei

3D structure databases

ProteinModelPortaliQ8IWU5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3731. Eukaryota.
COG3119. LUCA.
GeneTreeiENSGT00400000022041.
HOVERGENiHBG056431.
InParanoidiQ8IWU5.
KOiK14607.
OMAiGEACACD.
OrthoDBiEOG091G01S2.
PhylomeDBiQ8IWU5.
TreeFamiTF313545.

Family and domain databases

Gene3Di3.40.720.10. 3 hits.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR014615. Extracellular_sulfatase.
IPR024609. Extracellular_sulfatase_C.
IPR024607. Sulfatase_CS.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF12548. DUF3740. 1 hit.
PF00884. Sulfatase. 1 hit.
[Graphical view]
PIRSFiPIRSF036665. Sulf1. 1 hit.
SUPFAMiSSF53649. SSF53649. 3 hits.
PROSITEiPS00523. SULFATASE_1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8IWU5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGPPSLVLCL LSATVFSLLG GSSAFLSHHR LKGRFQRDRR NIRPNIILVL
60 70 80 90 100
TDDQDVELGS MQVMNKTRRI MEQGGAHFIN AFVTTPMCCP SRSSILTGKY
110 120 130 140 150
VHNHNTYTNN ENCSSPSWQA QHESRTFAVY LNSTGYRTAF FGKYLNEYNG
160 170 180 190 200
SYVPPGWKEW VGLLKNSRFY NYTLCRNGVK EKHGSDYSKD YLTDLITNDS
210 220 230 240 250
VSFFRTSKKM YPHRPVLMVI SHAAPHGPED SAPQYSRLFP NASQHITPSY
260 270 280 290 300
NYAPNPDKHW IMRYTGPMKP IHMEFTNMLQ RKRLQTLMSV DDSMETIYNM
310 320 330 340 350
LVETGELDNT YIVYTADHGY HIGQFGLVKG KSMPYEFDIR VPFYVRGPNV
360 370 380 390 400
EAGCLNPHIV LNIDLAPTIL DIAGLDIPAD MDGKSILKLL DTERPVNRFH
410 420 430 440 450
LKKKMRVWRD SFLVERGKLL HKRDNDKVDA QEENFLPKYQ RVKDLCQRAE
460 470 480 490 500
YQTACEQLGQ KWQCVEDATG KLKLHKCKGP MRLGGSRALS NLVPKYYGQG
510 520 530 540 550
SEACTCDSGD YKLSLAGRRK KLFKKKYKAS YVRSRSIRSV AIEVDGRVYH
560 570 580 590 600
VGLGDAAQPR NLTKRHWPGA PEDQDDKDGG DFSGTGGLPD YSAANPIKVT
610 620 630 640 650
HRCYILENDT VQCDLDLYKS LQAWKDHKLH IDHEIETLQN KIKNLREVRG
660 670 680 690 700
HLKKKRPEEC DCHKISYHTQ HKGRLKHRGS SLHPFRKGLQ EKDKVWLLRE
710 720 730 740 750
QKRKKKLRKL LKRLQNNDTC SMPGLTCFTH DNQHWQTAPF WTLGPFCACT
760 770 780 790 800
SANNNTYWCM RTINETHNFL FCEFATGFLE YFDLNTDPYQ LMNAVNTLDR
810 820 830 840 850
DVLNQLHVQL MELRSCKGYK QCNPRTRNMD LGLKDGGSYE QYRQFQRRKW
860 870
PEMKRPSSKS LGQLWEGWEG
Length:870
Mass (Da):100,455
Last modified:March 1, 2003 - v1
Checksum:i74B1069CE2774D73
GO
Isoform 2 (identifier: Q8IWU5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     832-834: Missing.

Note: May be due to a competing acceptor splice site.
Show »
Length:867
Mass (Da):100,156
Checksum:i1030E3CDAEC92A31
GO

Sequence cautioni

The sequence AAH20962 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAA86561 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAB61349 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06188576A → T.1 PublicationCorresponds to variant rs56218501dbSNPEnsembl.1
Natural variantiVAR_036494531Y → H in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_036495573D → N in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_052518674R → H.Corresponds to variant rs10048853dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013362832 – 834Missing in isoform 2. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY101176 mRNA. Translation: AAM76861.1.
AB033073 mRNA. Translation: BAA86561.2. Different initiation.
AY358461 mRNA. Translation: AAQ88826.1.
CR749319 mRNA. Translation: CAH18174.1.
AL354813, AL034418 Genomic DNA. Translation: CAI17943.1.
AL034418 Genomic DNA. Translation: CAC17694.1. Sequence problems.
AL034418, AL354813 Genomic DNA. Translation: CAI42143.1.
CH471077 Genomic DNA. Translation: EAW75690.1.
CH471077 Genomic DNA. Translation: EAW75693.1.
CH471077 Genomic DNA. Translation: EAW75694.1.
CH471077 Genomic DNA. Translation: EAW75695.1.
BC020962 mRNA. Translation: AAH20962.1. Different initiation.
BC110539 mRNA. Translation: AAI10540.1.
AL133001 mRNA. Translation: CAB61349.1. Different initiation.
CCDSiCCDS13408.1. [Q8IWU5-1]
CCDS13409.2. [Q8IWU5-2]
RefSeqiNP_001155313.1. NM_001161841.1. [Q8IWU5-1]
NP_061325.1. NM_018837.3. [Q8IWU5-1]
NP_940998.2. NM_198596.2. [Q8IWU5-2]
XP_005260515.1. XM_005260458.2. [Q8IWU5-1]
XP_006723893.1. XM_006723830.1. [Q8IWU5-1]
XP_016883444.1. XM_017027955.1. [Q8IWU5-1]
UniGeneiHs.162016.

Genome annotation databases

EnsembliENST00000359930; ENSP00000353007; ENSG00000196562. [Q8IWU5-1]
ENST00000467815; ENSP00000418442; ENSG00000196562. [Q8IWU5-2]
ENST00000484875; ENSP00000418290; ENSG00000196562. [Q8IWU5-1]
GeneIDi55959.
KEGGihsa:55959.
UCSCiuc002xto.4. human. [Q8IWU5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY101176 mRNA. Translation: AAM76861.1.
AB033073 mRNA. Translation: BAA86561.2. Different initiation.
AY358461 mRNA. Translation: AAQ88826.1.
CR749319 mRNA. Translation: CAH18174.1.
AL354813, AL034418 Genomic DNA. Translation: CAI17943.1.
AL034418 Genomic DNA. Translation: CAC17694.1. Sequence problems.
AL034418, AL354813 Genomic DNA. Translation: CAI42143.1.
CH471077 Genomic DNA. Translation: EAW75690.1.
CH471077 Genomic DNA. Translation: EAW75693.1.
CH471077 Genomic DNA. Translation: EAW75694.1.
CH471077 Genomic DNA. Translation: EAW75695.1.
BC020962 mRNA. Translation: AAH20962.1. Different initiation.
BC110539 mRNA. Translation: AAI10540.1.
AL133001 mRNA. Translation: CAB61349.1. Different initiation.
CCDSiCCDS13408.1. [Q8IWU5-1]
CCDS13409.2. [Q8IWU5-2]
RefSeqiNP_001155313.1. NM_001161841.1. [Q8IWU5-1]
NP_061325.1. NM_018837.3. [Q8IWU5-1]
NP_940998.2. NM_198596.2. [Q8IWU5-2]
XP_005260515.1. XM_005260458.2. [Q8IWU5-1]
XP_006723893.1. XM_006723830.1. [Q8IWU5-1]
XP_016883444.1. XM_017027955.1. [Q8IWU5-1]
UniGeneiHs.162016.

3D structure databases

ProteinModelPortaliQ8IWU5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121010. 17 interactors.
IntActiQ8IWU5. 2 interactors.
STRINGi9606.ENSP00000353007.

PTM databases

iPTMnetiQ8IWU5.
PhosphoSitePlusiQ8IWU5.

Polymorphism and mutation databases

BioMutaiSULF2.
DMDMi33112446.

Proteomic databases

MaxQBiQ8IWU5.
PaxDbiQ8IWU5.
PeptideAtlasiQ8IWU5.
PRIDEiQ8IWU5.
TopDownProteomicsiQ8IWU5-2. [Q8IWU5-2]

Protocols and materials databases

DNASUi55959.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000359930; ENSP00000353007; ENSG00000196562. [Q8IWU5-1]
ENST00000467815; ENSP00000418442; ENSG00000196562. [Q8IWU5-2]
ENST00000484875; ENSP00000418290; ENSG00000196562. [Q8IWU5-1]
GeneIDi55959.
KEGGihsa:55959.
UCSCiuc002xto.4. human. [Q8IWU5-1]

Organism-specific databases

CTDi55959.
DisGeNETi55959.
GeneCardsiSULF2.
H-InvDBHIX0027735.
HGNCiHGNC:20392. SULF2.
HPAiHPA002325.
MIMi610013. gene.
neXtProtiNX_Q8IWU5.
OpenTargetsiENSG00000196562.
PharmGKBiPA134902131.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3731. Eukaryota.
COG3119. LUCA.
GeneTreeiENSGT00400000022041.
HOVERGENiHBG056431.
InParanoidiQ8IWU5.
KOiK14607.
OMAiGEACACD.
OrthoDBiEOG091G01S2.
PhylomeDBiQ8IWU5.
TreeFamiTF313545.

Miscellaneous databases

ChiTaRSiSULF2. human.
GeneWikiiSULF2.
GenomeRNAii55959.
PROiQ8IWU5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000196562.
CleanExiHS_SULF2.
ExpressionAtlasiQ8IWU5. baseline and differential.
GenevisibleiQ8IWU5. HS.

Family and domain databases

Gene3Di3.40.720.10. 3 hits.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR014615. Extracellular_sulfatase.
IPR024609. Extracellular_sulfatase_C.
IPR024607. Sulfatase_CS.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF12548. DUF3740. 1 hit.
PF00884. Sulfatase. 1 hit.
[Graphical view]
PIRSFiPIRSF036665. Sulf1. 1 hit.
SUPFAMiSSF53649. SSF53649. 3 hits.
PROSITEiPS00523. SULFATASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSULF2_HUMAN
AccessioniPrimary (citable) accession number: Q8IWU5
Secondary accession number(s): E1P5U6
, Q5JYE1, Q6UX86, Q96SG2, Q9H1H0, Q9UJR3, Q9ULH3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.