Q8IWT3 (CUL9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cullin-9
Short name=CUL-9

Alternative name(s):
UbcH7-associated protein 1
p53-associated parkin-like cytoplasmic protein

Gene names

Name:	CUL9
Synonyms:	H7AP1, KIAA0708, PARC

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	2517 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytoplasmic anchor protein in p53-associated protein complex. Regulates the subcellular localization of p53 and subsequent function. Seems to be part of an atypical cullin-RING-based E3 ubiquitin-protein ligase complex. In vitro, complexes of CUL9/PARC with either CUL7 or TP53 contain E3 ubiquitin-protein ligase activity. Ref.10
Subunit structure	Forms a complex with p53/TP53 in the cytoplasm of unstressed cells. Interacts with UBCH7 and UBCH8. Interacts with CUL7 and RBX1. The CUL7-CUL9 heterodimer seems to interact specifically with TP53. Ref.1 Ref.9 Ref.10
Subcellular location	Cytoplasm.
Tissue specificity	Ubiquitously expressed in all tissues with highest expression in testis brain and kidney.
Domain	The IBR domain is required for interaction with UBCH7 and UBCH8.
Post-translational modification	In vitro, self-ubiquitination in the presence of E1, E2 and ubiquitin. Neddylated.
Sequence similarities	Belongs to the cullin family. Contains 1 DOC domain. Contains 1 IBR-type zinc finger. Contains 2 RING-type zinc fingers.
Sequence caution	The sequence BAC85207.1 differs from that shown. Reason: Intron retention. The sequence BAC86090.1 differs from that shown. Reason: Intron retention.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
PTM	Ubl conjugation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cullin-RING ubiquitin ligase complex Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TP53	P04637	2	EBI-311123,EBI-366083

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8IWT3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8IWT3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     418-527: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 2517

2517

Cullin-9

PRO_0000119815

Regions

Domain

1143 – 1322

180

DOC

Nucleotide binding

1363 – 1370

8

ATP Potential

Zinc finger

2070 – 2120

51

RING-type 1

Zinc finger

2140 – 2203

64

IBR-type

Zinc finger

2236 – 2265

30

RING-type 2

Coiled coil

1649 – 1691

43

Potential

Coiled coil

2365 – 2385

21

Potential

Compositional bias

1661 – 1689

29

Glu-rich

Natural variations

Alternative sequence

418 – 527

110

Missing in isoform 2.

VSP_009573

Natural variant

2058

1

H → P.
Corresponds to variant rs2273709 [ dbSNP | Ensembl ].

VAR_048844

Natural variant

2180

1

T → I.
Corresponds to variant rs11962520 [ dbSNP | Ensembl ].

VAR_048845

Experimental info

Sequence conflict

527

1

E → G in CAH18696. Ref.3

Sequence conflict

663

1

E → K in AAN61516. Ref.1

Sequence conflict

1171

1

E → K in CAH18328. Ref.3

Sequence conflict

1230

1

M → T in CAH18328. Ref.3

Sequence conflict

1268

1

N → S in CAH18328. Ref.3

Sequence conflict

1277

1

R → H in CAH18328. Ref.3

Sequence conflict

1830 – 1857

28

Missing in CAH18696. Ref.3

Sequence conflict

2049

1

L → P in CAH18696. Ref.3

Sequence conflict

2058 – 2059

2

HQ → PL in CAH18696. Ref.3

Sequence conflict

2345

1

V → VV in CAI20204. Ref.4

Sequence conflict

2345

1

V → VV in EAX04163. Ref.5

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

2517

Helix Strand Turn

Details...

Helix

376 – 382

7

Beta strand

388 – 393

6

Beta strand

403 – 408

6

Beta strand

411 – 413

3

Beta strand

416 – 420

5

Turn

421 – 424

4

Beta strand

425 – 429

5

Helix

431 – 433

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 4, 2005. Version 2. Checksum: 42387A55DCC52EAF	FASTA	2,517	281,229
	10 20 30 40 50 60 MVGERHAGDL MVPLGPRLQA YPEELIRQRP GHDGHPEYLI RWSVLKCGEV GKVGVEEGKA 70 80 90 100 110 120 EHILMWLSAP EVYANCPGLL GERALSKGLQ HEPAGVSGSF PRDPGGLDEV AMGEMEADVQ 130 140 150 160 170 180 ALVRRAARQL AESGTPSLTA AVLHTIHVLS AYASIGPLTG VFRETGALDL LMHMLCNPEP 190 200 210 220 230 240 QIRRSAGKML QALAAHDAGS RAHVLLSLSQ QDGIEQHMDF DSRYTLLELF AETTSSEEHC 250 260 270 280 290 300 MAFEGIHLPQ IPGKLLFSLV KRYLCVTSLL DQLNSSPELG AGDQSSPCAT REKSRGQREL 310 320 330 340 350 360 EFSMAVGNLI SELVRSMGWA RNLSEQGMSP PRPTRSIFQP YISGPSLLLP TIVTTPRRQG 370 380 390 400 410 420 WVFRQRSEFS SRSGYGEYVQ QTLQPGMRVR MLDDYEEISA GDEGEFRQSN NGIPPVQVFW 430 440 450 460 470 480 QSTGRTYWVH WHMLEILGPE EATEDKASAA VEKGAGATVL GTAFPSWDWN PMDGLYPLPY 490 500 510 520 530 540 LQPEPQKNER VGYLTQAEWW ELLFFIKKLD LCEQQPIFQN LWKNLDETLG EKALGEISVS 550 560 570 580 590 600 VEMAESLLQV LSSRFEGSTL NDLLNSQIYT KYGLLSNEPS SSSTSRNHSC TPDPEEESKS 610 620 630 640 650 660 EASFSEEETE SLKAKAEAPK TEAEPTKTRT ETPMAQSDSQ LFNQLLVTEG MTLPTEMKEA 670 680 690 700 710 720 ASEMARALRG PGPRSSLDQH VAAVVATVQI SSLDTNLQLS GLSALSQAVE EVTERDHPLV 730 740 750 760 770 780 RPDRSLREKL VKMLVELLTN QVGEKMVVVQ ALRLLYLLMT KHEWRPLFAR EGGIYAVLVC 790 800 810 820 830 840 MQEYKTSVLV QQAGLAALKM LAVASSSEIP TFVTGRDSIH SLFDAQMTRE IFASIDSATR 850 860 870 880 890 900 PGSESLLLTV PAAVILMLNT EGCSSAARNG LLLLNLLLCN HHTLGDQIIT QELRDTLFRH 910 920 930 940 950 960 SGIAPRTEPM PTTRTILMML LNRYSEPPGS PERAALETPI IQGQDGSPEL LIRSLVGGPS 970 980 990 1000 1010 1020 AELLLDLERV LCREGSPGGA VRPLLKRLQQ ETQPFLLLLR TLDAPGPNKT LLLSVLRVIT 1030 1040 1050 1060 1070 1080 RLLDFPEAMV LPWHEVLEPC LNCLSGPSSD SEIVQELTCF LHRLASMHKD YAVVLCCLGA 1090 1100 1110 1120 1130 1140 KEILSKVLDK HSAQLLLGCE LRDLVTECEK YAQLYSNLTS SILAGCIQMV LGQIEDHRRT 1150 1160 1170 1180 1190 1200 HQPINIPFFD VFLRHLCQGS SVEVKEDKCW EKVEVSSNPH RASKLTDHNP KTYWESNGST 1210 1220 1230 1240 1250 1260 GSHYITLHMH RGVLVRQLTL LVASEDSSYM PARVVVFGGD STSCIGTELN TVNVMPSASR 1270 1280 1290 1300 1310 1320 VILLENLNRF WPIIQIRIKR CQQGGIDTRV RGVEVLGPKP TFWPLFREQL CRRTCLFYTI 1330 1340 1350 1360 1370 1380 RAQAWSRDIA EDHRRLLQLC PRLNRVLRHE QNFADRFLPD DEAAQALGKT CWEALVSPLV 1390 1400 1410 1420 1430 1440 QNITSPDAEG VSALGWLLDQ YLEQRETSRN PLSRAASFAS RVRRLCHLLV HVEPPPGPSP 1450 1460 1470 1480 1490 1500 EPSTRPFSKN SKGRDRSPAP SPVLPSSSLR NITQCWLSVV QEQVSRFLAA AWRAPDFVPR 1510 1520 1530 1540 1550 1560 YCKLYEHLQR AGSELFGPRA AFMLALRSGF SGALLQQSFL TAAHMSEQFA RYIDQQIQGG 1570 1580 1590 1600 1610 1620 LIGGAPGVEM LGQLQRHLEP IMVLSGLELA TTFEHFYQHY MADRLLSFGS SWLEGAVLEQ 1630 1640 1650 1660 1670 1680 IGLCFPNRLP QLMLQSLSTS EELQRQFHLF QLQRLDKLFL EQEDEEEKRL EEEEEEEEEE 1690 1700 1710 1720 1730 1740 EAEKELFIED PSPAISILVL SPRCWPVSPL CYLYHPRKCL PTEFCDALDR FSSFYSQSQN 1750 1760 1770 1780 1790 1800 HPVLDMGPHR RLQWTWLGRA ELQFGKQILH VSTVQMWLLL KFNQTEEVSV ETLLKDSDLS 1810 1820 1830 1840 1850 1860 PELLLQALVP LTSGNGPLTL HEGQDFPHGG VLRLHEPGPQ RSGEALWLIP PQAYLNVEKD 1870 1880 1890 1900 1910 1920 EGRTLEQKRN LLSCLLVRIL KAHGEKGLHI DQLVCLVLEA WQKGPNPPGT LGHTVAGGVA 1930 1940 1950 1960 1970 1980 CTSTDVLSCI LHLLGQGYVK RRDDRPQILM YAAPEPMGPC RGQADVPFCG SQSETSKPSP 1990 2000 2010 2020 2030 2040 EAVATLASLQ LPAGRTMSPQ EVEGLMKQTV RQVQETLNLE PDVAQHLLAH SHWGAEQLLQ 2050 2060 2070 2080 2090 2100 SYSEDPEPLL LAAGLCVHQA QAVPVRPDHC PVCVSPLGCD DDLPSLCCMH YCCKSCWNEY 2110 2120 2130 2140 2150 2160 LTTRIEQNLV LNCTCPIADC PAQPTGAFIR AIVSSPEVIS KYEKALLRGY VESCSNLTWC 2170 2180 2190 2200 2210 2220 TNPQGCDRIL CRQGLGCGTT CSKCGWASCF NCSFPEAHYP ASCGHMSQWV DDGGYYDGMS 2230 2240 2250 2260 2270 2280 VEAQSKHLAK LISKRCPSCQ APIEKNEGCL HMTCAKCNHG FCWRCLKSWK PNHKDYYNCS 2290 2300 2310 2320 2330 2340 AMVSKAARQE KRFQDYNERC TFHHQAREFA VNLRNRVSAI HEVPPPRSFT FLNDACQGLE 2350 2360 2370 2380 2390 2400 QARKVLAYAC VYSFYSQDAE YMDVVEQQTE NLELHTNALQ ILLEETLLRC RDLASSLRLL 2410 2420 2430 2440 2450 2460 RADCLSTGME LLRRIQERLL AILQHSAQDF RVGLQSPSVE AWEAKGPNMP GSQPQASSGP 2470 2480 2490 2500 2510 EAEEEEEDDE DDVPEWQQDE FDEELDNDSF SYDESENLDQ ETFFFGDEEE DEDEAYD
	Isoform 2 [UniParc]. Checksum: F41FAF09CC71656C	FASTA	2,407	268,362
	10 20 30 40 50 60 MVGERHAGDL MVPLGPRLQA YPEELIRQRP GHDGHPEYLI RWSVLKCGEV GKVGVEEGKA 70 80 90 100 110 120 EHILMWLSAP EVYANCPGLL GERALSKGLQ HEPAGVSGSF PRDPGGLDEV AMGEMEADVQ 130 140 150 160 170 180 ALVRRAARQL AESGTPSLTA AVLHTIHVLS AYASIGPLTG VFRETGALDL LMHMLCNPEP 190 200 210 220 230 240 QIRRSAGKML QALAAHDAGS RAHVLLSLSQ QDGIEQHMDF DSRYTLLELF AETTSSEEHC 250 260 270 280 290 300 MAFEGIHLPQ IPGKLLFSLV KRYLCVTSLL DQLNSSPELG AGDQSSPCAT REKSRGQREL 310 320 330 340 350 360 EFSMAVGNLI SELVRSMGWA RNLSEQGMSP PRPTRSIFQP YISGPSLLLP TIVTTPRRQG 370 380 390 400 410 420 WVFRQRSEFS SRSGYGEYVQ QTLQPGMRVR MLDDYEEISA GDEGEFRQSN NGIPPVQTLG 430 440 450 460 470 480 EKALGEISVS VEMAESLLQV LSSRFEGSTL NDLLNSQIYT KYGLLSNEPS SSSTSRNHSC 490 500 510 520 530 540 TPDPEEESKS EASFSEEETE SLKAKAEAPK TEAEPTKTRT ETPMAQSDSQ LFNQLLVTEG 550 560 570 580 590 600 MTLPTEMKEA ASEMARALRG PGPRSSLDQH VAAVVATVQI SSLDTNLQLS GLSALSQAVE 610 620 630 640 650 660 EVTERDHPLV RPDRSLREKL VKMLVELLTN QVGEKMVVVQ ALRLLYLLMT KHEWRPLFAR 670 680 690 700 710 720 EGGIYAVLVC MQEYKTSVLV QQAGLAALKM LAVASSSEIP TFVTGRDSIH SLFDAQMTRE 730 740 750 760 770 780 IFASIDSATR PGSESLLLTV PAAVILMLNT EGCSSAARNG LLLLNLLLCN HHTLGDQIIT 790 800 810 820 830 840 QELRDTLFRH SGIAPRTEPM PTTRTILMML LNRYSEPPGS PERAALETPI IQGQDGSPEL 850 860 870 880 890 900 LIRSLVGGPS AELLLDLERV LCREGSPGGA VRPLLKRLQQ ETQPFLLLLR TLDAPGPNKT 910 920 930 940 950 960 LLLSVLRVIT RLLDFPEAMV LPWHEVLEPC LNCLSGPSSD SEIVQELTCF LHRLASMHKD 970 980 990 1000 1010 1020 YAVVLCCLGA KEILSKVLDK HSAQLLLGCE LRDLVTECEK YAQLYSNLTS SILAGCIQMV 1030 1040 1050 1060 1070 1080 LGQIEDHRRT HQPINIPFFD VFLRHLCQGS SVEVKEDKCW EKVEVSSNPH RASKLTDHNP 1090 1100 1110 1120 1130 1140 KTYWESNGST GSHYITLHMH RGVLVRQLTL LVASEDSSYM PARVVVFGGD STSCIGTELN 1150 1160 1170 1180 1190 1200 TVNVMPSASR VILLENLNRF WPIIQIRIKR CQQGGIDTRV RGVEVLGPKP TFWPLFREQL 1210 1220 1230 1240 1250 1260 CRRTCLFYTI RAQAWSRDIA EDHRRLLQLC PRLNRVLRHE QNFADRFLPD DEAAQALGKT 1270 1280 1290 1300 1310 1320 CWEALVSPLV QNITSPDAEG VSALGWLLDQ YLEQRETSRN PLSRAASFAS RVRRLCHLLV 1330 1340 1350 1360 1370 1380 HVEPPPGPSP EPSTRPFSKN SKGRDRSPAP SPVLPSSSLR NITQCWLSVV QEQVSRFLAA 1390 1400 1410 1420 1430 1440 AWRAPDFVPR YCKLYEHLQR AGSELFGPRA AFMLALRSGF SGALLQQSFL TAAHMSEQFA 1450 1460 1470 1480 1490 1500 RYIDQQIQGG LIGGAPGVEM LGQLQRHLEP IMVLSGLELA TTFEHFYQHY MADRLLSFGS 1510 1520 1530 1540 1550 1560 SWLEGAVLEQ IGLCFPNRLP QLMLQSLSTS EELQRQFHLF QLQRLDKLFL EQEDEEEKRL 1570 1580 1590 1600 1610 1620 EEEEEEEEEE EAEKELFIED PSPAISILVL SPRCWPVSPL CYLYHPRKCL PTEFCDALDR 1630 1640 1650 1660 1670 1680 FSSFYSQSQN HPVLDMGPHR RLQWTWLGRA ELQFGKQILH VSTVQMWLLL KFNQTEEVSV 1690 1700 1710 1720 1730 1740 ETLLKDSDLS PELLLQALVP LTSGNGPLTL HEGQDFPHGG VLRLHEPGPQ RSGEALWLIP 1750 1760 1770 1780 1790 1800 PQAYLNVEKD EGRTLEQKRN LLSCLLVRIL KAHGEKGLHI DQLVCLVLEA WQKGPNPPGT 1810 1820 1830 1840 1850 1860 LGHTVAGGVA CTSTDVLSCI LHLLGQGYVK RRDDRPQILM YAAPEPMGPC RGQADVPFCG 1870 1880 1890 1900 1910 1920 SQSETSKPSP EAVATLASLQ LPAGRTMSPQ EVEGLMKQTV RQVQETLNLE PDVAQHLLAH 1930 1940 1950 1960 1970 1980 SHWGAEQLLQ SYSEDPEPLL LAAGLCVHQA QAVPVRPDHC PVCVSPLGCD DDLPSLCCMH 1990 2000 2010 2020 2030 2040 YCCKSCWNEY LTTRIEQNLV LNCTCPIADC PAQPTGAFIR AIVSSPEVIS KYEKALLRGY 2050 2060 2070 2080 2090 2100 VESCSNLTWC TNPQGCDRIL CRQGLGCGTT CSKCGWASCF NCSFPEAHYP ASCGHMSQWV 2110 2120 2130 2140 2150 2160 DDGGYYDGMS VEAQSKHLAK LISKRCPSCQ APIEKNEGCL HMTCAKCNHG FCWRCLKSWK 2170 2180 2190 2200 2210 2220 PNHKDYYNCS AMVSKAARQE KRFQDYNERC TFHHQAREFA VNLRNRVSAI HEVPPPRSFT 2230 2240 2250 2260 2270 2280 FLNDACQGLE QARKVLAYAC VYSFYSQDAE YMDVVEQQTE NLELHTNALQ ILLEETLLRC 2290 2300 2310 2320 2330 2340 RDLASSLRLL RADCLSTGME LLRRIQERLL AILQHSAQDF RVGLQSPSVE AWEAKGPNMP 2350 2360 2370 2380 2390 2400 GSQPQASSGP EAEEEEEDDE DDVPEWQQDE FDEELDNDSF SYDESENLDQ ETFFFGDEEE DEDEAYD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Parc: a cytoplasmic anchor for p53." Nikolaev A.Y., Li M., Puskas N., Qin J., Gu W. Cell 112:29-40(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 786-791; 2419-2431 AND 2432-2445, INTERACTION WITH TP53. Tissue: Cervix carcinoma.
[2]	"UbcH7 associated protein, H7AP1. A new member of the Parkin/Ariadne E3 ubiquitin Ligase (PAUL) family." Rose S.A., Ardley H.C., Tan N.G., Scott G.B., Markham A.F., Robinson P.A. Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Testis.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Amygdala and Testis.
[4]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-662 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 528-662 (ISOFORMS 1/2). Tissue: Chondrocyte and Thymus.
[7]	"Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Ishikawa K., Nagase T., Suyama M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:169-176(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 765-2517 (ISOFORM 1). Tissue: Brain.
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1554-2517 (ISOFORM 1). Tissue: Lung.
[9]	"The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with RING finger/IBR motif-containing domains of HHARI and H7-AP1." Moynihan T.P., Ardley H.C., Nuber U., Rose S.A., Jones P.F., Markham A.F., Scheffner M., Robinson P.A. J. Biol. Chem. 274:30963-30968(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UBCH7 AND UBCH8.
[10]	"PARC and CUL7 form atypical cullin RING ligase complexes." Skaar J.R., Florens L., Tsutsumi T., Arai T., Tron A., Swanson S.K., Washburn M.P., DeCaprio J.A. Cancer Res. 67:2006-2014(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CUL7; RBX1 AND TP53, NEDDYLATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY145132 mRNA. Translation: AAN61516.1.
AJ318215 mRNA. Translation: CAC85756.1.
CR749511 mRNA. Translation: CAH18328.1.
CR749841 mRNA. Translation: CAH18696.1.
AL133375 Genomic DNA. Translation: CAI20204.1.
CH471081 Genomic DNA. Translation: EAX04163.1.
AK125228 mRNA. Translation: BAC86090.1. Sequence problems.
AK129649 mRNA. Translation: BAC85207.1. Sequence problems.
AB014608 mRNA. Translation: BAA31683.3.
BC002879 mRNA. Translation: AAH02879.2.

IPI

IPI00402732.
IPI00744001.

PIR

T00350.

RefSeq

NP_055904.1. NM_015089.2.

UniGene

Hs.485434.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2JUF	NMR	-	A	366-466	[»]

ProteinModelPortal

Q8IWT3.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q8IWT3. 7 interactions.

MINT

MINT-6615845.

STRING

9606.ENSP00000252050.

PTM databases

PhosphoSite

Q8IWT3.

Polymorphism databases

DMDM

57015409.

Proteomic databases

PaxDb

Q8IWT3.

PRIDE

Q8IWT3.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000252050; ENSP00000252050; ENSG00000112659.
ENST00000354495; ENSP00000346490; ENSG00000112659.

GeneID

23113.

KEGG

hsa:23113.

UCSC

uc003ouk.3. human.

Organism-specific databases

CTD

23113.

GeneCards

GC06P043149.

HGNC

HGNC:15982. CUL9.

HPA

HPA016434.

MIM

607489. gene.

neXtProt

NX_Q8IWT3.

PharmGKB

PA164718328.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG48148.

KO

K11970.

OMA

TKHEWRP.

OrthoDB

EOG4P2Q1C.

PhylomeDB

Q8IWT3.

Gene expression databases

ArrayExpress

Q8IWT3.

Bgee

Q8IWT3.

Genevestigator

Q8IWT3.

GermOnline

ENSG00000112659. Homo sapiens.

Family and domain databases

Gene3D

1.10.10.10. 1 hit.
1.25.10.10. 4 hits.
2.30.30.30. 1 hit.

InterPro

IPR004939. APC_su10/DOC_dom.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021097. CPH_domain.
IPR016157. Cullin_CS.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR019559. Cullin_neddylation_domain.
IPR008979. Galactose-bd-like.
IPR014722. Rib_L2_dom2.
IPR011991. WHTH_DNA-bd_dom.
IPR002867. Znf_C6HC.
IPR001841. Znf_RING.
IPR017907. Znf_RING_CS.
[Graphical view]

Pfam

PF03256. APC10. 1 hit.
PF11515. Cul7. 1 hit.
PF00888. Cullin. 1 hit.
PF01485. IBR. 2 hits.
[Graphical view]

SMART

SM00884. Cullin_Nedd8. 1 hit.
SM00647. IBR. 2 hits.
SM00184. RING. 2 hits.
[Graphical view]

SUPFAM

SSF48371. ARM-type_fold. 1 hit.
SSF75632. Cullin_homology. 1 hit.
SSF49785. Gal_bind_like. 1 hit.

PROSITE

PS01256. CULLIN_1. 1 hit.
PS50069. CULLIN_2. 1 hit.
PS51284. DOC. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

CUL9. human.

EvolutionaryTrace

Q8IWT3.

GenomeRNAi

23113.

NextBio

44317.

SOURCE

Search...

Entry information

Entry name

CUL9_HUMAN

Accession

Primary (citable) accession number: Q8IWT3
Secondary accession number(s): O75188 , Q5TCY3, Q68CP2, Q68D92, Q8N3W9, Q9BU56

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	January 4, 2005
Last modified:	May 1, 2013
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.