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Protein

Protein archease

Gene

ZBTB8OS

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB. Together with DDX1, acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi39CalciumBy similarity1
Metal bindingi166CalciumBy similarity1
Metal bindingi167Calcium; via carboxylateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-6784531. tRNA processing in the nucleus.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein archease
Alternative name(s):
Protein ZBTB8OS
Zinc finger and BTB domain-containing opposite strand protein 8
Gene namesi
Name:ZBTB8OS
Synonyms:ARCH
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:24094. ZBTB8OS.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • nucleoplasm Source: Reactome
  • tRNA-splicing ligase complex Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi39D → A: Abolishes ability to activate tRNA ligase activity of RTCB. 1 Publication1
Mutagenesisi144K → A: Abolishes ability to activate tRNA ligase activity of RTCB. 1 Publication1

Organism-specific databases

DisGeNETi339487.
PharmGKBiPA142670540.

Polymorphism and mutation databases

DMDMi146286037.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002859502 – 167Protein archeaseAdd BLAST166

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8IWT0.
MaxQBiQ8IWT0.
PaxDbiQ8IWT0.
PeptideAtlasiQ8IWT0.
PRIDEiQ8IWT0.
TopDownProteomicsiQ8IWT0-1. [Q8IWT0-1]
Q8IWT0-2. [Q8IWT0-2]

PTM databases

iPTMnetiQ8IWT0.
PhosphoSitePlusiQ8IWT0.

Expressioni

Gene expression databases

BgeeiENSG00000176261.
CleanExiHS_ZBTB8OS.
ExpressionAtlasiQ8IWT0. baseline and differential.
GenevisibleiQ8IWT0. HS.

Organism-specific databases

HPAiHPA044835.
HPA045317.
HPA046315.
HPA046331.

Interactioni

Subunit structurei

Component of the tRNA-splicing ligase complex.1 Publication

Protein-protein interaction databases

BioGridi130893. 7 interactors.
DIPiDIP-61031N.
IntActiQ8IWT0. 3 interactors.
STRINGi9606.ENSP00000417677.

Structurei

3D structure databases

ProteinModelPortaliQ8IWT0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the archease family.Curated

Phylogenomic databases

eggNOGiKOG4528. Eukaryota.
COG1371. LUCA.
HOVERGENiHBG104905.
InParanoidiQ8IWT0.
PhylomeDBiQ8IWT0.

Family and domain databases

Gene3Di3.55.10.10. 1 hit.
InterProiIPR002804. Archease.
IPR023572. Archease_dom.
[Graphical view]
PANTHERiPTHR12682. PTHR12682. 1 hit.
PfamiPF01951. Archease. 1 hit.
[Graphical view]
ProDomiPD012969. PD012969. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF69819. SSF69819. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8IWT0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQEEEDVRD YNLTEEQKAI KAKYPPVNRK YEYLDHTADV QLHAWGDTLE
60 70 80 90 100
EAFEQCAMAM FGYMTDTGTV EPLQTVEVET QGDDLQSLLF HFLDEWLYKF
110 120 130 140 150
SADEFFIPRE VKVLSIDQRN FKLRSIGWGE EFSLSKHPQG TEVKAITYSA
160
MQVYNEENPE VFVIIDI
Length:167
Mass (Da):19,491
Last modified:May 1, 2007 - v2
Checksum:i26EC7F03CA7B42F1
GO
Isoform 2 (identifier: Q8IWT0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     110-167: EVKVLSIDQR...NPEVFVIIDI → VGRRIFIVQAPSGNRSQSNNIFSNAGL

Show »
Length:136
Mass (Da):15,709
Checksum:i6B166BC23296478F
GO

Sequence cautioni

The sequence AAM34477 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAN75223 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAN75224 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAI20020 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAI20021 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51E → V in AAM34478 (Ref. 1) Curated1
Sequence conflicti76V → A in AAM34478 (Ref. 1) Curated1
Sequence conflicti93L → S in AAM34478 (Ref. 1) Curated1
Sequence conflicti119R → G in AAM34478 (Ref. 1) Curated1
Sequence conflicti137H → R in AAM34478 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_024926110 – 167EVKVL…VIIDI → VGRRIFIVQAPSGNRSQSNN IFSNAGL in isoform 2. 2 PublicationsAdd BLAST58

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY099493 mRNA. Translation: AAM34477.1. Different initiation.
AY099494 mRNA. Translation: AAM34478.1.
AY151084 mRNA. Translation: AAN75223.1. Different initiation.
AY151085 mRNA. Translation: AAN75224.1. Different initiation.
AL033529, AC114489 Genomic DNA. Translation: CAI20020.1. Different initiation.
AL033529, AC114489 Genomic DNA. Translation: CAI20021.1. Different initiation.
BC058843 mRNA. Translation: AAH58843.1.
RefSeqiNP_001295065.1. NM_001308136.1.
NP_001295068.1. NM_001308139.1.
NP_848642.1. NM_178547.3.
UniGeneiHs.655921.

Genome annotation databases

EnsembliENST00000373501; ENSP00000362600; ENSG00000176261.
GeneIDi339487.
KEGGihsa:339487.
UCSCiuc057elg.1. human. [Q8IWT0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY099493 mRNA. Translation: AAM34477.1. Different initiation.
AY099494 mRNA. Translation: AAM34478.1.
AY151084 mRNA. Translation: AAN75223.1. Different initiation.
AY151085 mRNA. Translation: AAN75224.1. Different initiation.
AL033529, AC114489 Genomic DNA. Translation: CAI20020.1. Different initiation.
AL033529, AC114489 Genomic DNA. Translation: CAI20021.1. Different initiation.
BC058843 mRNA. Translation: AAH58843.1.
RefSeqiNP_001295065.1. NM_001308136.1.
NP_001295068.1. NM_001308139.1.
NP_848642.1. NM_178547.3.
UniGeneiHs.655921.

3D structure databases

ProteinModelPortaliQ8IWT0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi130893. 7 interactors.
DIPiDIP-61031N.
IntActiQ8IWT0. 3 interactors.
STRINGi9606.ENSP00000417677.

PTM databases

iPTMnetiQ8IWT0.
PhosphoSitePlusiQ8IWT0.

Polymorphism and mutation databases

DMDMi146286037.

Proteomic databases

EPDiQ8IWT0.
MaxQBiQ8IWT0.
PaxDbiQ8IWT0.
PeptideAtlasiQ8IWT0.
PRIDEiQ8IWT0.
TopDownProteomicsiQ8IWT0-1. [Q8IWT0-1]
Q8IWT0-2. [Q8IWT0-2]

Protocols and materials databases

DNASUi339487.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000373501; ENSP00000362600; ENSG00000176261.
GeneIDi339487.
KEGGihsa:339487.
UCSCiuc057elg.1. human. [Q8IWT0-1]

Organism-specific databases

CTDi339487.
DisGeNETi339487.
GeneCardsiZBTB8OS.
H-InvDBHIX0028669.
HGNCiHGNC:24094. ZBTB8OS.
HPAiHPA044835.
HPA045317.
HPA046315.
HPA046331.
neXtProtiNX_Q8IWT0.
PharmGKBiPA142670540.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4528. Eukaryota.
COG1371. LUCA.
HOVERGENiHBG104905.
InParanoidiQ8IWT0.
PhylomeDBiQ8IWT0.

Enzyme and pathway databases

ReactomeiR-HSA-6784531. tRNA processing in the nucleus.

Miscellaneous databases

GenomeRNAii339487.
PROiQ8IWT0.

Gene expression databases

BgeeiENSG00000176261.
CleanExiHS_ZBTB8OS.
ExpressionAtlasiQ8IWT0. baseline and differential.
GenevisibleiQ8IWT0. HS.

Family and domain databases

Gene3Di3.55.10.10. 1 hit.
InterProiIPR002804. Archease.
IPR023572. Archease_dom.
[Graphical view]
PANTHERiPTHR12682. PTHR12682. 1 hit.
PfamiPF01951. Archease. 1 hit.
[Graphical view]
ProDomiPD012969. PD012969. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF69819. SSF69819. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiARCH_HUMAN
AccessioniPrimary (citable) accession number: Q8IWT0
Secondary accession number(s): Q5TGK5
, Q6PDA1, Q8IWS9, Q8NEV6, Q8NEV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: November 2, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.