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Protein

Protein archease

Gene

ZBTB8OS

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB. Together with DDX1, acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi39CalciumBy similarity1
Metal bindingi166CalciumBy similarity1
Metal bindingi167Calcium; via carboxylateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB

Keywordsi

Biological processtRNA processing
LigandCalcium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000176261-MONOMER
ReactomeiR-HSA-6784531 tRNA processing in the nucleus

Names & Taxonomyi

Protein namesi
Recommended name:
Protein archease
Alternative name(s):
Protein ZBTB8OS
Zinc finger and BTB domain-containing opposite strand protein 8
Gene namesi
Name:ZBTB8OS
Synonyms:ARCH
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000176261.15
HGNCiHGNC:24094 ZBTB8OS
neXtProtiNX_Q8IWT0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi39D → A: Abolishes ability to activate tRNA ligase activity of RTCB. 1 Publication1
Mutagenesisi144K → A: Abolishes ability to activate tRNA ligase activity of RTCB. 1 Publication1

Organism-specific databases

DisGeNETi339487
PharmGKBiPA142670540

Polymorphism and mutation databases

DMDMi146286037

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002859502 – 167Protein archeaseAdd BLAST166

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8IWT0
MaxQBiQ8IWT0
PaxDbiQ8IWT0
PeptideAtlasiQ8IWT0
PRIDEiQ8IWT0
TopDownProteomicsiQ8IWT0-1 [Q8IWT0-1]
Q8IWT0-2 [Q8IWT0-2]

PTM databases

iPTMnetiQ8IWT0
PhosphoSitePlusiQ8IWT0

Expressioni

Gene expression databases

BgeeiENSG00000176261
CleanExiHS_ZBTB8OS
ExpressionAtlasiQ8IWT0 baseline and differential
GenevisibleiQ8IWT0 HS

Organism-specific databases

HPAiHPA046315

Interactioni

Subunit structurei

Component of the tRNA-splicing ligase complex.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RTCBQ9Y3I02EBI-2808825,EBI-2107208

Protein-protein interaction databases

BioGridi130893, 7 interactors
DIPiDIP-61031N
IntActiQ8IWT0, 6 interactors
STRINGi9606.ENSP00000417677

Structurei

3D structure databases

ProteinModelPortaliQ8IWT0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the archease family.Curated

Phylogenomic databases

eggNOGiKOG4528 Eukaryota
COG1371 LUCA
HOVERGENiHBG104905
InParanoidiQ8IWT0
PhylomeDBiQ8IWT0

Family and domain databases

Gene3Di3.55.10.10, 1 hit
InterProiView protein in InterPro
IPR002804 Archease
IPR023572 Archease_dom
IPR036820 Archease_dom_sf
PANTHERiPTHR12682 PTHR12682, 1 hit
PfamiView protein in Pfam
PF01951 Archease, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD012969 PD012969, 1 hit
SUPFAMiSSF69819 SSF69819, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8IWT0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQEEEDVRD YNLTEEQKAI KAKYPPVNRK YEYLDHTADV QLHAWGDTLE
60 70 80 90 100
EAFEQCAMAM FGYMTDTGTV EPLQTVEVET QGDDLQSLLF HFLDEWLYKF
110 120 130 140 150
SADEFFIPRE VKVLSIDQRN FKLRSIGWGE EFSLSKHPQG TEVKAITYSA
160
MQVYNEENPE VFVIIDI
Length:167
Mass (Da):19,491
Last modified:May 1, 2007 - v2
Checksum:i26EC7F03CA7B42F1
GO
Isoform 2 (identifier: Q8IWT0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     110-167: EVKVLSIDQR...NPEVFVIIDI → VGRRIFIVQAPSGNRSQSNNIFSNAGL

Show »
Length:136
Mass (Da):15,709
Checksum:i6B166BC23296478F
GO

Sequence cautioni

The sequence AAM34477 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAN75223 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAN75224 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAI20020 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAI20021 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51E → V in AAM34478 (Ref. 1) Curated1
Sequence conflicti76V → A in AAM34478 (Ref. 1) Curated1
Sequence conflicti93L → S in AAM34478 (Ref. 1) Curated1
Sequence conflicti119R → G in AAM34478 (Ref. 1) Curated1
Sequence conflicti137H → R in AAM34478 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_024926110 – 167EVKVL…VIIDI → VGRRIFIVQAPSGNRSQSNN IFSNAGL in isoform 2. 2 PublicationsAdd BLAST58

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY099493 mRNA Translation: AAM34477.1 Different initiation.
AY099494 mRNA Translation: AAM34478.1
AY151084 mRNA Translation: AAN75223.1 Different initiation.
AY151085 mRNA Translation: AAN75224.1 Different initiation.
AL033529, AC114489 Genomic DNA Translation: CAI20020.1 Different initiation.
AL033529, AC114489 Genomic DNA Translation: CAI20021.1 Different initiation.
BC058843 mRNA Translation: AAH58843.1
RefSeqiNP_001295065.1, NM_001308136.1
NP_001295068.1, NM_001308139.1
NP_848642.1, NM_178547.3
UniGeneiHs.655921

Genome annotation databases

EnsembliENST00000373501; ENSP00000362600; ENSG00000176261
GeneIDi339487
KEGGihsa:339487
UCSCiuc057elg.1 human [Q8IWT0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiARCH_HUMAN
AccessioniPrimary (citable) accession number: Q8IWT0
Secondary accession number(s): Q5TGK5
, Q6PDA1, Q8IWS9, Q8NEV6, Q8NEV7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: May 23, 2018
This is version 114 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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