Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Adhesion G protein-coupled receptor A3

Gene

ADGRA3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Orphan receptor that may have a role in planar cell polarity pathway.By similarity

GO - Molecular functioni

  • G-protein coupled receptor activity Source: GDB

GO - Biological processi

  • cell surface receptor signaling pathway Source: InterPro
  • G-protein coupled receptor signaling pathway Source: GDB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Protein family/group databases

MEROPSiP02.018.

Names & Taxonomyi

Protein namesi
Recommended name:
Adhesion G protein-coupled receptor A31 Publication
Alternative name(s):
G-protein coupled receptor 125
Gene namesi
Name:ADGRA3Imported
Synonyms:GPR125
ORF Names:UNQ556/PRO1113
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:13839. ADGRA3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini34 – 761ExtracellularCuratedAdd BLAST728
Transmembranei762 – 782Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini783 – 796CytoplasmicCuratedAdd BLAST14
Transmembranei797 – 817Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini818 – 826ExtracellularCurated9
Transmembranei827 – 847Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini848 – 876CytoplasmicCuratedAdd BLAST29
Transmembranei877 – 897Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini898 – 919ExtracellularCuratedAdd BLAST22
Transmembranei920 – 940Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini941 – 996CytoplasmicCuratedAdd BLAST56
Transmembranei997 – 1017Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini1018 – 1024ExtracellularCurated7
Transmembranei1025 – 1045Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini1046 – 1321CytoplasmicCuratedAdd BLAST276

GO - Cellular componenti

  • external side of plasma membrane Source: Ensembl
  • integral component of membrane Source: GDB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi166647.
OpenTargetsiENSG00000152990.
PharmGKBiPA134919579.

Polymorphism and mutation databases

DMDMi50400542.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33Sequence analysisAdd BLAST33
ChainiPRO_000001290034 – 1321Adhesion G protein-coupled receptor A3Add BLAST1288

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi81N-linked (GlcNAc...)Sequence analysis1
Glycosylationi98N-linked (GlcNAc...)Sequence analysis1
Glycosylationi159N-linked (GlcNAc...)Sequence analysis1
Glycosylationi206N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi264 ↔ 324PROSITE-ProRule annotation
Glycosylationi301N-linked (GlcNAc...)Sequence analysis1
Glycosylationi332N-linked (GlcNAc...)Sequence analysis1
Glycosylationi433N-linked (GlcNAc...)Sequence analysis1
Glycosylationi453N-linked (GlcNAc...)Sequence analysis1
Glycosylationi592N-linked (GlcNAc...)Sequence analysis1
Glycosylationi652N-linked (GlcNAc...)Sequence analysis1
Glycosylationi687N-linked (GlcNAc...)Sequence analysis1
Glycosylationi728N-linked (GlcNAc...)Sequence analysis1
Glycosylationi821N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ8IWK6.
PaxDbiQ8IWK6.
PeptideAtlasiQ8IWK6.
PRIDEiQ8IWK6.

PTM databases

iPTMnetiQ8IWK6.
PhosphoSitePlusiQ8IWK6.

Expressioni

Gene expression databases

BgeeiENSG00000152990.
CleanExiHS_GPR125.
ExpressionAtlasiQ8IWK6. baseline and differential.
GenevisibleiQ8IWK6. HS.

Organism-specific databases

HPAiHPA008904.

Interactioni

Subunit structurei

Interacts (via PDZ-binding motif) with DLG1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
DLG1Q12959-22EBI-10949249,EBI-357500

Protein-protein interaction databases

BioGridi127930. 1 interactor.
IntActiQ8IWK6. 1 interactor.
STRINGi9606.ENSP00000334952.

Structurei

3D structure databases

ProteinModelPortaliQ8IWK6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati82 – 103LRR 1Add BLAST22
Repeati106 – 127LRR 2Add BLAST22
Repeati130 – 151LRR 3Add BLAST22
Repeati154 – 175LRR 4Add BLAST22
Domaini187 – 237LRRCTAdd BLAST51
Domaini242 – 340Ig-likeAdd BLAST99
Repeati594 – 620LRR 5Add BLAST27
Domaini698 – 749GPSPROSITE-ProRule annotationAdd BLAST52

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1319 – 1321PDZ-bindingSequence analysis3

Sequence similaritiesi

Contains 1 GPS domain.PROSITE-ProRule annotation
Contains 5 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated

Keywords - Domaini

Immunoglobulin domain, Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
KOG4237. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00860000133701.
HOGENOMiHOG000112767.
HOVERGENiHBG051777.
InParanoidiQ8IWK6.
KOiK08462.
OMAiPVIVCGI.
OrthoDBiEOG091G03GB.
PhylomeDBiQ8IWK6.
TreeFamiTF331206.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR000832. GPCR_2_secretin-like.
IPR000203. GPS.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF01825. GPS. 1 hit.
PF07679. I-set. 1 hit.
PF13855. LRR_8. 1 hit.
[Graphical view]
SMARTiSM00303. GPS. 1 hit.
SM00409. IG. 1 hit.
SM00369. LRR_TYP. 4 hits.
SM00082. LRRCT. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF52058. SSF52058. 1 hit.
PROSITEiPS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
PS50835. IG_LIKE. 1 hit.
PS51450. LRR. 4 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8IWK6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPPGRRRGR AQPPLLLPLS LLALLALLGG GGGGGAAALP AGCKHDGRPR
60 70 80 90 100
GAGRAAGAAE GKVVCSSLEL AQVLPPDTLP NRTVTLILSN NKISELKNGS
110 120 130 140 150
FSGLSLLERL DLRNNLISSI DPGAFWGLSS LKRLDLTNNR IGCLNADIFR
160 170 180 190 200
GLTNLVRLNL SGNLFSSLSQ GTFDYLASLR SLEFQTEYLL CDCNILWMHR
210 220 230 240 250
WVKEKNITVR DTRCVYPKSL QAQPVTGVKQ ELLTCDPPLE LPSFYMTPSH
260 270 280 290 300
RQVVFEGDSL PFQCMASYID QDMQVLWYQD GRIVETDESQ GIFVEKNMIH
310 320 330 340 350
NCSLIASALT ISNIQAGSTG NWGCHVQTKR GNNTRTVDIV VLESSAQYCP
360 370 380 390 400
PERVVNNKGD FRWPRTLAGI TAYLQCTRNT HGSGIYPGNP QDERKAWRRC
410 420 430 440 450
DRGGFWADDD YSRCQYANDV TRVLYMFNQM PLNLTNAVAT ARQLLAYTVE
460 470 480 490 500
AANFSDKMDV IFVAEMIEKF GRFTKEEKSK ELGDVMVDIA SNIMLADERV
510 520 530 540 550
LWLAQREAKA CSRIVQCLQR IATYRLAGGA HVYSTYSPNI ALEAYVIKST
560 570 580 590 600
GFTGMTCTVF QKVAASDRTG LSDYGRRDPE GNLDKQLSFK CNVSNTFSSL
610 620 630 640 650
ALKNTIVEAS IQLPPSLFSP KQKRELRPTD DSLYKLQLIA FRNGKLFPAT
660 670 680 690 700
GNSTNLADDG KRRTVVTPVI LTKIDGVNVD THHIPVNVTL RRIAHGADAV
710 720 730 740 750
AARWDFDLLN GQGGWKSDGC HILYSDENIT TIQCYSLSNY AVLMDLTGSE
760 770 780 790 800
LYTQAASLLH PVVYTTAIIL LLCLLAVIVS YIYHHSLIRI SLKSWHMLVN
810 820 830 840 850
LCFHIFLTCV VFVGGITQTR NASICQAVGI ILHYSTLATV LWVGVTARNI
860 870 880 890 900
YKQVTKKAKR CQDPDEPPPP PRPMLRFYLI GGGIPIIVCG ITAAANIKNY
910 920 930 940 950
GSRPNAPYCW MAWEPSLGAF YGPASFITFV NCMYFLSIFI QLKRHPERKY
960 970 980 990 1000
ELKEPTEEQQ RLAANENGEI NHQDSMSLSL ISTSALENEH TFHSQLLGAS
1010 1020 1030 1040 1050
LTLLLYVALW MFGALAVSLY YPLDLVFSFV FGATSLSFSA FFVVHHCVNR
1060 1070 1080 1090 1100
EDVRLAWIMT CCPGRSSYSV QVNVQPPNSN GTNGEAPKCP NSSAESSCTN
1110 1120 1130 1140 1150
KSASSFKNSS QGCKLTNLQA AAAQCHANSL PLNSTPQLDN SLTEHSMDND
1160 1170 1180 1190 1200
IKMHVAPLEV QFRTNVHSSR HHKNRSKGHR ASRLTVLREY AYDVPTSVEG
1210 1220 1230 1240 1250
SVQNGLPKSR LGNNEGHSRS RRAYLAYRER QYNPPQQDSS DACSTLPKSS
1260 1270 1280 1290 1300
RNFEKPVSTT SKKDALRKPA VVELENQQKS YGLNLAIQNG PIKSNGQEGP
1310 1320
LLGTDSTGNV RTGLWKHETT V
Length:1,321
Mass (Da):146,151
Last modified:July 19, 2004 - v2
Checksum:i25EBC1620E1895EA
GO
Isoform 2 (identifier: Q8IWK6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     604-616: NTIVEASIQLPPS → VCYILQSFKTIYS
     617-1321: Missing.

Note: No experimental confirmation available.
Show »
Length:616
Mass (Da):68,243
Checksum:i00CEB483C5714F80
GO
Isoform 3 (identifier: Q8IWK6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-134: MEPPGRRRGR...FWGLSSLKRL → MMRVMPCQV
     603-620: Missing.

Note: No experimental confirmation available.
Show »
Length:1,178
Mass (Da):131,543
Checksum:i8C46F040B84B26E9
GO
Isoform 4 (identifier: Q8IWK6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MEPPGRRRGR → MPLKRNNSKY
     11-236: Missing.
     604-607: NTIV → GKFR
     608-1321: Missing.

Note: No experimental confirmation available.
Show »
Length:381
Mass (Da):42,973
Checksum:i56F63AE58F94AFEC
GO

Sequence cautioni

The sequence AAH35645 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti537S → P in BC026009 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0339711043V → M.Corresponds to variant rs9002dbSNPEnsembl.1
Natural variantiVAR_0339721166V → G.Corresponds to variant rs3814416dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0107381 – 134MEPPG…SLKRL → MMRVMPCQV in isoform 3. 1 PublicationAdd BLAST134
Alternative sequenceiVSP_0107431 – 10MEPPGRRRGR → MPLKRNNSKY in isoform 4. 1 Publication10
Alternative sequenceiVSP_01074411 – 236Missing in isoform 4. 1 PublicationAdd BLAST226
Alternative sequenceiVSP_010740603 – 620Missing in isoform 3. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_010741604 – 616NTIVE…QLPPS → VCYILQSFKTIYS in isoform 2. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_010745604 – 607NTIV → GKFR in isoform 4. 1 Publication4
Alternative sequenceiVSP_010746608 – 1321Missing in isoform 4. 1 PublicationAdd BLAST714
Alternative sequenceiVSP_010742617 – 1321Missing in isoform 2. 1 PublicationAdd BLAST705

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY181243 mRNA. Translation: AAO27355.1.
AY358306 mRNA. Translation: AAQ88673.1.
AC093735 Genomic DNA. No translation available.
AC093814 Genomic DNA. No translation available.
BC026009 mRNA. No translation available.
BC035645 mRNA. Translation: AAH35645.1. Sequence problems.
CCDSiCCDS33964.1. [Q8IWK6-1]
RefSeqiNP_660333.2. NM_145290.3. [Q8IWK6-1]
UniGeneiHs.684429.
Hs.99195.

Genome annotation databases

EnsembliENST00000334304; ENSP00000334952; ENSG00000152990. [Q8IWK6-1]
ENST00000502482; ENSP00000421006; ENSG00000152990. [Q8IWK6-2]
ENST00000508133; ENSP00000422606; ENSG00000152990. [Q8IWK6-4]
GeneIDi166647.
KEGGihsa:166647.
UCSCiuc003gqm.3. human. [Q8IWK6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY181243 mRNA. Translation: AAO27355.1.
AY358306 mRNA. Translation: AAQ88673.1.
AC093735 Genomic DNA. No translation available.
AC093814 Genomic DNA. No translation available.
BC026009 mRNA. No translation available.
BC035645 mRNA. Translation: AAH35645.1. Sequence problems.
CCDSiCCDS33964.1. [Q8IWK6-1]
RefSeqiNP_660333.2. NM_145290.3. [Q8IWK6-1]
UniGeneiHs.684429.
Hs.99195.

3D structure databases

ProteinModelPortaliQ8IWK6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127930. 1 interactor.
IntActiQ8IWK6. 1 interactor.
STRINGi9606.ENSP00000334952.

Protein family/group databases

MEROPSiP02.018.
GPCRDBiSearch...

PTM databases

iPTMnetiQ8IWK6.
PhosphoSitePlusiQ8IWK6.

Polymorphism and mutation databases

DMDMi50400542.

Proteomic databases

EPDiQ8IWK6.
PaxDbiQ8IWK6.
PeptideAtlasiQ8IWK6.
PRIDEiQ8IWK6.

Protocols and materials databases

DNASUi166647.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000334304; ENSP00000334952; ENSG00000152990. [Q8IWK6-1]
ENST00000502482; ENSP00000421006; ENSG00000152990. [Q8IWK6-2]
ENST00000508133; ENSP00000422606; ENSG00000152990. [Q8IWK6-4]
GeneIDi166647.
KEGGihsa:166647.
UCSCiuc003gqm.3. human. [Q8IWK6-1]

Organism-specific databases

CTDi166647.
DisGeNETi166647.
GeneCardsiADGRA3.
HGNCiHGNC:13839. ADGRA3.
HPAiHPA008904.
MIMi612303. gene.
neXtProtiNX_Q8IWK6.
OpenTargetsiENSG00000152990.
PharmGKBiPA134919579.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
KOG4237. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00860000133701.
HOGENOMiHOG000112767.
HOVERGENiHBG051777.
InParanoidiQ8IWK6.
KOiK08462.
OMAiPVIVCGI.
OrthoDBiEOG091G03GB.
PhylomeDBiQ8IWK6.
TreeFamiTF331206.

Miscellaneous databases

ChiTaRSiGPR125. human.
GeneWikiiGPR125.
GenomeRNAii166647.
PROiQ8IWK6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000152990.
CleanExiHS_GPR125.
ExpressionAtlasiQ8IWK6. baseline and differential.
GenevisibleiQ8IWK6. HS.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR000832. GPCR_2_secretin-like.
IPR000203. GPS.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF01825. GPS. 1 hit.
PF07679. I-set. 1 hit.
PF13855. LRR_8. 1 hit.
[Graphical view]
SMARTiSM00303. GPS. 1 hit.
SM00409. IG. 1 hit.
SM00369. LRR_TYP. 4 hits.
SM00082. LRRCT. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF52058. SSF52058. 1 hit.
PROSITEiPS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
PS50835. IG_LIKE. 1 hit.
PS51450. LRR. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGRA3_HUMAN
AccessioniPrimary (citable) accession number: Q8IWK6
Secondary accession number(s): Q6UXK9, Q86SQ5, Q8TC55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: November 30, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Most adhesion GPCRs proteins undergo autoproteolysis at the GPS domain. ADGRA3 is predicted non-cleavable because of the lack of a consensus catalytic triad sequence within GPS domain.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.