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Q8IWK6 (GP125_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable G-protein coupled receptor 125
Gene names
Name:GPR125
ORF Names:UNQ556/PRO1113
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1321 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Orphan receptor.

Subunit structure

Interacts with DLG1. Ref.5

Subcellular location

Cell membrane; Multi-pass membrane protein.

Domain

Binds to DLG1 through its PDZ-binding motif By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.

Contains 1 GPS domain.

Contains 1 Ig-like (immunoglobulin-like) domain.

Contains 5 LRR (leucine-rich) repeats.

Contains 1 LRRCT domain.

Sequence caution

The sequence AAH35645.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8IWK6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8IWK6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     604-616: NTIVEASIQLPPS → VCYILQSFKTIYS
     617-1321: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q8IWK6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-134: MEPPGRRRGR...FWGLSSLKRL → MMRVMPCQV
     603-620: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q8IWK6-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MEPPGRRRGR → MPLKRNNSKY
     11-236: Missing.
     604-607: NTIV → GKFR
     608-1321: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3333 Potential
Chain34 – 13211288Probable G-protein coupled receptor 125
PRO_0000012900

Regions

Topological domain34 – 761728Extracellular Potential
Transmembrane762 – 78221Helical; Name=1; Potential
Topological domain783 – 79614Cytoplasmic Potential
Transmembrane797 – 81721Helical; Name=2; Potential
Topological domain818 – 8269Extracellular Potential
Transmembrane827 – 84721Helical; Name=3; Potential
Topological domain848 – 87629Cytoplasmic Potential
Transmembrane877 – 89721Helical; Name=4; Potential
Topological domain898 – 91922Extracellular Potential
Transmembrane920 – 94021Helical; Name=5; Potential
Topological domain941 – 99656Cytoplasmic Potential
Transmembrane997 – 101721Helical; Name=6; Potential
Topological domain1018 – 10247Extracellular Potential
Transmembrane1025 – 104521Helical; Name=7; Potential
Topological domain1046 – 1321276Cytoplasmic Potential
Repeat82 – 10322LRR 1
Repeat106 – 12722LRR 2
Repeat130 – 15122LRR 3
Repeat154 – 17522LRR 4
Domain187 – 23751LRRCT
Domain242 – 34099Ig-like
Repeat594 – 62027LRR 5
Domain698 – 74952GPS
Motif1319 – 13213PDZ-binding Potential

Amino acid modifications

Glycosylation811N-linked (GlcNAc...) Potential
Glycosylation981N-linked (GlcNAc...) Potential
Glycosylation1591N-linked (GlcNAc...) Potential
Glycosylation2061N-linked (GlcNAc...) Potential
Glycosylation3011N-linked (GlcNAc...) Potential
Glycosylation3321N-linked (GlcNAc...) Potential
Glycosylation4331N-linked (GlcNAc...) Potential
Glycosylation4531N-linked (GlcNAc...) Potential
Glycosylation5921N-linked (GlcNAc...) Potential
Glycosylation6521N-linked (GlcNAc...) Potential
Glycosylation6871N-linked (GlcNAc...) Potential
Glycosylation7281N-linked (GlcNAc...) Potential
Glycosylation8211N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 134134MEPPG…SLKRL → MMRVMPCQV in isoform 3.
VSP_010738
Alternative sequence1 – 1010MEPPGRRRGR → MPLKRNNSKY in isoform 4.
VSP_010743
Alternative sequence11 – 236226Missing in isoform 4.
VSP_010744
Alternative sequence603 – 62018Missing in isoform 3.
VSP_010740
Alternative sequence604 – 61613NTIVE…QLPPS → VCYILQSFKTIYS in isoform 2.
VSP_010741
Alternative sequence604 – 6074NTIV → GKFR in isoform 4.
VSP_010745
Alternative sequence608 – 1321714Missing in isoform 4.
VSP_010746
Alternative sequence617 – 1321705Missing in isoform 2.
VSP_010742
Natural variant10431V → M.
Corresponds to variant rs9002 [ dbSNP | Ensembl ].
VAR_033971
Natural variant11661V → G.
Corresponds to variant rs3814416 [ dbSNP | Ensembl ].
VAR_033972

Experimental info

Sequence conflict5371S → P in BC026009. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 19, 2004. Version 2.
Checksum: 25EBC1620E1895EA

FASTA1,321146,151
        10         20         30         40         50         60 
MEPPGRRRGR AQPPLLLPLS LLALLALLGG GGGGGAAALP AGCKHDGRPR GAGRAAGAAE 

        70         80         90        100        110        120 
GKVVCSSLEL AQVLPPDTLP NRTVTLILSN NKISELKNGS FSGLSLLERL DLRNNLISSI 

       130        140        150        160        170        180 
DPGAFWGLSS LKRLDLTNNR IGCLNADIFR GLTNLVRLNL SGNLFSSLSQ GTFDYLASLR 

       190        200        210        220        230        240 
SLEFQTEYLL CDCNILWMHR WVKEKNITVR DTRCVYPKSL QAQPVTGVKQ ELLTCDPPLE 

       250        260        270        280        290        300 
LPSFYMTPSH RQVVFEGDSL PFQCMASYID QDMQVLWYQD GRIVETDESQ GIFVEKNMIH 

       310        320        330        340        350        360 
NCSLIASALT ISNIQAGSTG NWGCHVQTKR GNNTRTVDIV VLESSAQYCP PERVVNNKGD 

       370        380        390        400        410        420 
FRWPRTLAGI TAYLQCTRNT HGSGIYPGNP QDERKAWRRC DRGGFWADDD YSRCQYANDV 

       430        440        450        460        470        480 
TRVLYMFNQM PLNLTNAVAT ARQLLAYTVE AANFSDKMDV IFVAEMIEKF GRFTKEEKSK 

       490        500        510        520        530        540 
ELGDVMVDIA SNIMLADERV LWLAQREAKA CSRIVQCLQR IATYRLAGGA HVYSTYSPNI 

       550        560        570        580        590        600 
ALEAYVIKST GFTGMTCTVF QKVAASDRTG LSDYGRRDPE GNLDKQLSFK CNVSNTFSSL 

       610        620        630        640        650        660 
ALKNTIVEAS IQLPPSLFSP KQKRELRPTD DSLYKLQLIA FRNGKLFPAT GNSTNLADDG 

       670        680        690        700        710        720 
KRRTVVTPVI LTKIDGVNVD THHIPVNVTL RRIAHGADAV AARWDFDLLN GQGGWKSDGC 

       730        740        750        760        770        780 
HILYSDENIT TIQCYSLSNY AVLMDLTGSE LYTQAASLLH PVVYTTAIIL LLCLLAVIVS 

       790        800        810        820        830        840 
YIYHHSLIRI SLKSWHMLVN LCFHIFLTCV VFVGGITQTR NASICQAVGI ILHYSTLATV 

       850        860        870        880        890        900 
LWVGVTARNI YKQVTKKAKR CQDPDEPPPP PRPMLRFYLI GGGIPIIVCG ITAAANIKNY 

       910        920        930        940        950        960 
GSRPNAPYCW MAWEPSLGAF YGPASFITFV NCMYFLSIFI QLKRHPERKY ELKEPTEEQQ 

       970        980        990       1000       1010       1020 
RLAANENGEI NHQDSMSLSL ISTSALENEH TFHSQLLGAS LTLLLYVALW MFGALAVSLY 

      1030       1040       1050       1060       1070       1080 
YPLDLVFSFV FGATSLSFSA FFVVHHCVNR EDVRLAWIMT CCPGRSSYSV QVNVQPPNSN 

      1090       1100       1110       1120       1130       1140 
GTNGEAPKCP NSSAESSCTN KSASSFKNSS QGCKLTNLQA AAAQCHANSL PLNSTPQLDN 

      1150       1160       1170       1180       1190       1200 
SLTEHSMDND IKMHVAPLEV QFRTNVHSSR HHKNRSKGHR ASRLTVLREY AYDVPTSVEG 

      1210       1220       1230       1240       1250       1260 
SVQNGLPKSR LGNNEGHSRS RRAYLAYRER QYNPPQQDSS DACSTLPKSS RNFEKPVSTT 

      1270       1280       1290       1300       1310       1320 
SKKDALRKPA VVELENQQKS YGLNLAIQNG PIKSNGQEGP LLGTDSTGNV RTGLWKHETT 


V 

« Hide

Isoform 2 [UniParc].

Checksum: 00CEB483C5714F80
Show »

FASTA61668,243
Isoform 3 [UniParc].

Checksum: 8C46F040B84B26E9
Show »

FASTA1,178131,543
Isoform 4 [UniParc].

Checksum: 56F63AE58F94AFEC
Show »

FASTA38142,973

References

« Hide 'large scale' references
[1]"There exist at least 30 human G-protein-coupled receptors with long Ser/Thr-rich N-termini."
Fredriksson R., Gloriam D.E.I., Hoeglund P.J., Lagerstroem M.C., Schioeth H.B.
Biochem. Biophys. Res. Commun. 301:725-734(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[2]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
Tissue: Eye and Liver.
[5]"Direct binding of the human homologue of the Drosophila disc large tumor suppressor gene to seven-pass transmembrane proteins, tumor endothelial marker 5 (TEM5), and a novel TEM5-like protein."
Yamamoto Y., Irie K., Asada M., Mino A., Mandai K., Takai Y.
Oncogene 23:3889-3897(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DLG1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY181243 mRNA. Translation: AAO27355.1.
AY358306 mRNA. Translation: AAQ88673.1.
AC093735 Genomic DNA. No translation available.
AC093814 Genomic DNA. No translation available.
BC026009 mRNA. No translation available.
BC035645 mRNA. Translation: AAH35645.1. Sequence problems.
CCDSCCDS33964.1. [Q8IWK6-1]
RefSeqNP_660333.2. NM_145290.3. [Q8IWK6-1]
UniGeneHs.99195.

3D structure databases

ProteinModelPortalQ8IWK6.
SMRQ8IWK6. Positions 36-339.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid127930. 1 interaction.
STRING9606.ENSP00000334952.

Protein family/group databases

MEROPSS63.021.
GPCRDBSearch...

PTM databases

PhosphoSiteQ8IWK6.

Polymorphism databases

DMDM50400542.

Proteomic databases

PaxDbQ8IWK6.
PRIDEQ8IWK6.

Protocols and materials databases

DNASU166647.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000334304; ENSP00000334952; ENSG00000152990. [Q8IWK6-1]
ENST00000502482; ENSP00000421006; ENSG00000152990. [Q8IWK6-2]
ENST00000508133; ENSP00000422606; ENSG00000152990. [Q8IWK6-4]
GeneID166647.
KEGGhsa:166647.
UCSCuc003gqm.2. human. [Q8IWK6-1]
uc003gqn.1. human. [Q8IWK6-4]
uc003gqo.4. human. [Q8IWK6-2]
uc010ieo.2. human. [Q8IWK6-3]

Organism-specific databases

CTD166647.
GeneCardsGC04M022346.
HGNCHGNC:13839. GPR125.
HPAHPA008904.
MIM612303. gene.
neXtProtNX_Q8IWK6.
PharmGKBPA134919579.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG307919.
HOGENOMHOG000112767.
HOVERGENHBG051777.
InParanoidQ8IWK6.
KOK08462.
OMAHHIPVNV.
OrthoDBEOG7RV9F8.
PhylomeDBQ8IWK6.
TreeFamTF331206.

Gene expression databases

ArrayExpressQ8IWK6.
BgeeQ8IWK6.
CleanExHS_GPR125.
GenevestigatorQ8IWK6.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR000483. Cys-rich_flank_reg_C.
IPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR000832. GPCR_2_secretin-like.
IPR000203. GPS.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PfamPF00002. 7tm_2. 1 hit.
PF01825. GPS. 1 hit.
PF02793. HRM. 1 hit.
PF07679. I-set. 1 hit.
PF13855. LRR_8. 1 hit.
[Graphical view]
SMARTSM00303. GPS. 1 hit.
SM00409. IG. 1 hit.
SM00369. LRR_TYP. 3 hits.
SM00082. LRRCT. 1 hit.
[Graphical view]
PROSITEPS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
PS50835. IG_LIKE. 1 hit.
PS51450. LRR. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGPR125. human.
GeneWikiGPR125.
GenomeRNAi166647.
NextBio88603.
PROQ8IWK6.
SOURCESearch...

Entry information

Entry nameGP125_HUMAN
AccessionPrimary (citable) accession number: Q8IWK6
Secondary accession number(s): Q6UXK9, Q86SQ5, Q8TC55
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: July 9, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries